Applied Cell Biology最新文献

{"title":"Protective Effect of Qi-Shield on the Regeneration of Cultured Connective Tissue Fibroblasts after Mobile Phone Radiation – A Study Using Continuous Monitoring by Video Micrography","authors":"P. Dartsch","doi":"10.53043/2320-1991.acb90026","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90026","url":null,"abstract":"Background: Mobile phones are high performance devices that consume a lot of energy and also generate heat in form of microwaves. This heat is mainly created by the high frequency electromagnetic fields that transmit voice or data in mobile communications. Moreover, mobile phones also possess non-thermal radiation which has been shown to induce oxidative stress and molecular and cellular damage. Material and methods: In this study not only the dynamic process of cellular damage following oxidative stress from the radiation of an actively transmitting mobile phone was investigated, but also a device named Qi-Shield, which claims to protect from the adverse effects of a mobile phone & WLAN radiation at realistic conditions. The regeneration process of cultured connective tissue cells (cell line L-929) which results in the closure of an artificial cell-free area by cell proliferation and migration, was used for continuous video micrography evaluation for more than 60 hours. Three different experimental approaches were conducted: (1) Regeneration of untreated control cells; (2) Regeneration of unprotected cells after 4 hours of mobile phone radiation; (3) Regeneration of Qi-Shield protected cells after 4 hours of mobile phone radiation. For definite time points during the regeneration process the residual cell-free area was examined by a specialized software with artificial intelligence. Four independent experiments over a period of four weeks were conducted. Results and conclusions: A quite consistent cell reaction for the three different experimental approaches was observed in all experiments. The unexposed control cells showed a nearly linear reduction of the cell-free area with a time point of closure at 50 hours. The unprotected cells after mobile phone exposure also showed a roughly linear reduction of the cell-free area, but its magnitude was significantly lower and did not result in any closure of the cell-free area. The cells protected by Qi-Shield behaved very close to the unexposed cells, although a closure of the cell-free area within 60 hours of regeneration was only observed in two of four experiments. When calculating a relation between the values at 50 hours of regeneration by setting the value for the reduction of unexposed control cells as 100 %, a reduction value of 22 % was obtained for the unprotected cells, but 82.7 % for the Qi-Shield protected cells. Therefore, Qi-Shield was able to protect cells from the adverse effects of mobile phone & WLAN radiation by more than 82 %. The results clearly demonstrate the different dynamic reactions of connective tissue cells after mobile phone radiation and the successful use of Qi-Shield to reduce adverse cellular effects. Keywords: Mobile phone radiation; Oxidative stress; Fibroblast; L-929; Cell regeneration; Cell culture","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"231 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124480658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial Effects of “Frequency Specific Microcurrent“ on Regeneration of Cultured Connective Tissue Fibroblasts","authors":"P. Dartsch","doi":"10.53043/2320-1991.acb90025","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90025","url":null,"abstract":"Regeneration of complex structures after injury requires dramatic changes in cellular behaviour. One of the main functions of early signaling events after injury is the production of additional cells that are able to rebuild lost or damaged structures. This is mostly done by cell proliferation. Another fundamental cellular event is the migration of cells, especially in exogenous electrical fields. In this present in vitro study, we examined the effect of Frequency Specific Microcurrent (FSM) of the TimeWaver Frequency McMakin system on regeneration/wound healing process. By using a specific bioassay, the granulation phase, which is characterized by the occurrence of cell migration and proliferation, was reconstructed. The study was conducted with cultured connective tissue fibroblasts (L-929) which were seeded into cell cultures plates containing silicon frames which represent a cell-free space (= artificial wound) after the cell layer has become confluent and the frame was removed. The cells were allowed to migrate and proliferate into the cellfree space for closure. After fixation and staining, the residual cell-free space was calculated by a specialized software. Untreated controls were compared with cells which have been exposed once for 5 to 6 min to two different programs of the Time Waver Frequency McMakin system. Three independent experiments with a total of 12 single measurements were conducted. Morphological evaluation of cell regeneration/wound healing in comparison to the untreated control showed a significant improvement in the colonization of the cell-free space by the application of both programs with only a single short-term treatment of the TimeWaver Frequency McMakin system. Calculation of the residual spaces after 24 hours of fibroblast migration and proliferation into the cell-free space by a specialized software showed a residual cell-free space of 22.1 ± 3.8 % for the untreated control, whereas the residual cell-free space was only 9.6 ± 3.6 % for cells after exposure to program # 1 (= trauma/re-activation) and 15.7 ± 1.5 % for cells after exposure to programm # 2 (= cell regeneration/wound healing). All data represent mean value ± standard deviation. This means that the cells have been stimulated significantly for an improved regeneration process by migration and proliferation after only a single short-term exposure to the programs used in this study. The TimeWaver Frequency McMakin system has clearly demonstrated its beneficial effect in experimental test procedures at the cellular level. The application of both programs resulted in a significant improvement in regeneration/wound healing of connective tissue fibroblasts even after only a single short-term application. The present cellular results confirm previous findings from practical use of high-performance athletes during regenerative processes due to physical exercise or overload. Keywords: Microcurrent therapy; Cell regeneration; Wound healing; Connective tissue fibr","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115181701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cells May Differentiate to Microorganisms","authors":"A. Salerian","doi":"10.53043/2320-1991.acb90022","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90022","url":null,"abstract":"This study advances a previous hypotheses, “Human body may produce bacteria “and proposes that some infections are generated by human stem cells which differentiate to bacteria and fungus. Evidence consistent with the hypotheses that” Stem cells may differentiate to microorganisms” include studies suggesting that Christensenellaceae are heritable, human genetics shape gut microbiome, host genetics control the composition of gut microbiota and Tinea Versicolor skin infections caused by Malassezia species. Furthermore it has been demonstrated that, amniotic fluid, breast tissue and milk, placenta, umbilical cord blood, meconium harbor bacteria that are not contaminants. Because human stem cells have the properties to differentiate to microorganisms, they seem to be the most likely candidates to differentiate to microorganisms. Evidence suggests humans and other complex multi cellular organisms are giant sources of new microorganisms. Further experimental validation of this observation is necessary yet its potential benefits to study novel avenues to combat opportunistic infections may make it worthy of further scientific scrutiny in the near future.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"124 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113940108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in house qPCR System to Evaluate Transcripts Stimulated by an Immune Innate Agonist (CIGB2020) during the Covid19 Pandemic Scenario","authors":"J. Aguiar, José Angel Silva, Omar Gel, R. Estrada, F. Freyre, Chabeli Rodríguez, E. Pentón, G. Guillén, Julio Cesar Aguilar","doi":"10.53043/2320-1991.acb90024","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90024","url":null,"abstract":"Background: A low cost and simple real-time PCR method to quantify different transcripts in human samples is a need in most developing countries. A quantitative real-time PCR assay to evaluate the relative RNA expression stimulation of specific transcripts during the Pandemic COVID19 scenario was developed at the Center for Genetic Engineering and Biotechnology in Havana, using a commercial amplification system with standards and specific primers that are produced in house. Methods: Oro-pharyngeal scrape samples from patients that were treated previously with an innate immune agonist, the CIGB2020, were employed to RNA purification, cDNA synthesis, and evaluation of different gene transcripts by the in house qPCR assay using different standard curves constructed with specific primers that amplify a pool of the own evaluated transcripts. Results: Preliminary results showed a typical standard curve from the in house qPCR system developed. A typical standard curve during the evaluation of the TLR3 gene transcript was described as example, and was calculated automatically by plotting the Ct values against each standard of known concentration, and the linear regression of this curve was also calculated as: M=-3.31641, B=42.73084, R=0.9516. It was also observed the influence of the CIGB2020 on the stimulation of the three toll like receptors from the innate immune system. Conclusions: This study demonstrated that the in house qPCR system is useful to quantify the relative induction of the expression of several genes transcripts considered as immunological markers of the immune innate system","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121428129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoantibodies in Females Exposed to Indoor Air Dampness Microbiota and Complaining of Electromagnetic Hypersensitivity- The Case Control Report","authors":"T. Tuuminen, Itai Katz, K. Vaali, H. Heidecke, G. Halpert, H. Amital, Y. Shoenfeld","doi":"10.53043/2320-1991.acb90023","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90023","url":null,"abstract":"We hypothesized that prolonged or cumulative exposure to indoor air dampness microbiota in moisture-damaged buildings and daily exposure to wireless telecommunication devices would potentiate the risk of electromagnetic hypersensitivity (EHS), which is poorly defined. We performed a nested comparative analysis within an age- and sex-matched study of females who were exposed to dampness microbiota with self-reported complaints compatible with EHS (n=11). Their levels of autoantibodies towards 13 different autoantigens were measured. EHS presented as multiple chemical sensitivity, profound fatigue, memory disturbances in all subjects (11/11), and cognitive impairment in the majority (9/11). When comparing the patients to controls, no difference was detected between the levels of the following autoantibodies: angiotensin II type 1 receptor (AGTR1), endothelin receptor type A (ETAR), adrenergic receptors α1AR, α2AR, β1AR, β2AR and cholinergic muscarinic receptors m1AChR, m2AChR, m3AChR and m5AChR. In contrast, IgG levels towards m4AChR and fibroblast growth factor receptor 3 (FGFR3), and IgM autoantibodies against glycosylated moieties of heparan and heparan sulphate (TS-HDS) were significantly decreased in the study cohort, p=0.008; p=0.032, p<0.001, respectively. This is the first report demonstrating an imbalance in the nervous system autoantibodies in patients with EHS. The clinical significance of these altered responses remains to be clarified.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128587033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method to Improve Administered Cells Via Intramuscular Route to Augment Efficacy of Regenerative Cell Therapy in The Field of Peripheral Artery Disease","authors":"Keisuke Miyake, S. Miyagawa, Y. Sawa","doi":"10.53043/2320-1991.acb90020","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90020","url":null,"abstract":"In the field of peripheral artery disease (PAD), regenerative cell therapy has been expected to be a new alternative therapy especially for severely diseased patients. Although various preclinical studies showed promising results, those therapies failed to show sufficient efficacy for actual PAD patients and current guideline do not recommend regenerative therapy for PAD. The disappointing result may be caused by low retention rate of administered substance. Recently we published an article regarding novel method to augment the effect of cell therapy. In this review article, we would like to focus on the importance of improvement in cell survival and prerequisite factors to develop effective regenerative therapy for PAD patients.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127179890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Learning Nutrition from Nutrient Sciences of History","authors":"Y. Gao-Balch","doi":"10.53043/2320-1991.acb90018","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90018","url":null,"abstract":"McCollum and Davis started to observe that the lab rats eat some food to lead to poor growth, so they want to prove the reason for failure in development. Experimenting with two different rats’ diets, added to only butterfat and an additional extra of the diet supplement. Result added to the addition of the supplement of diet is to heal and expect growth (Mcllum, 1917). In another story, Chemist Casimir Funk noticed the symptoms of beriberi in chickens. Then he used chickens in the laboratory to feed leftover rice with processed until a new cook changed brown rice. The chickens soon recovered from beriberi. The birds got well again a few days after switching to brown rice to birds. Eijkman thought that processing rice lacked a dietary component found in brown rice. The beriberi caused by an absence in the dietary ingredient, which he called “the anti-beriberi factor.” Korsakoff studied the effects of alcoholism on the nervous system and drew attention to several cases of alcoholic polyneuropathy with distinctive mental symptoms (Korsakoff’s syndrome) due to the defense of Thiamin. Finally, Eijkman proved that the disease was not caused by blood contamination, respiratory metabolism, or temperature variation. Eventually, it was determined that the missing compound causing beriberi was vitamin B1, whose name is Thiamine. Eijkman with Sir Frederick Hopkins won the 1929 Nobel Prize for Medicine; interest in extracted food nutrients began in the latter half of the 19th century with Thiamin’s first vitamin. Thiamin was water-soluble. vitamiN B2 Following Thiamine of the discovery, researchers realized that one or more additional water-soluble factors called the vitamin B2 complex. Observing a pigment in milk with yellow-green, whole wheat, yeast, and liver began by chemist Alexander Wynter Blyth in 1872. Until late 1991, Riboflavin was the second vitamin to be isolated. They thought no nutritional disease causes riboflavin deficiency until they experimented with young Rats to feed with the food extract and observed that the young growth is outstanding to prove Riboflavin’s growth-stimulating properties. Riboflavin is the precursor of the coenzymes Flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). Several oxidation-reduction (redox) reactions are involved in energy production, and numerous metabolic pathways act as electron carriers. Riboflavin deficiency can affect multiple paths in the metabolism of vitamin B6, folate, niacin, and iron. Riboflavin deficiency has been linked to preeclampsia in pregnant women. This condition may progress to eclampsia and cause severe bleeding and death. The risk of preeclampsia has recently been associated with the presence of a genetic variant (C677T) in the methylenetetrahydrofolate reductase (MTHFR) gene. This gene codes for the MTHFR enzyme, which is FAD-dependent.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"27 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113985707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional Therapy by Leveraging our Knowledge of Nutrigenomics and Nutrigenetics","authors":"Y. Gao-Balch","doi":"10.53043/2320-1991.acb90019","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90019","url":null,"abstract":"Nutrigenomics is a tool to measure gene expressions in response to dietary intake and better understand the human body's nutrient-gene interactions. Nutrigenetics is a tool to assess how genetic variations influence nutrients' interaction result of developing various chronic diseases. Dietary factors alter genes' expression, including nutritional factors such as antioxidants, vitamins, phytochemicals, caffeine, sterols, fatty acids, and alcohol. Furthermore, genetic polymorphisms in nutrient action targets such as enzymes could alter molecular pathways that influence the physiological response to dietary intake. Identifying relevant diet-gene interactions will benefit individuals seeking personalized nutritional advice.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121283553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of Gut Microbiota to Improve Sleep Dysfunction for Children with Autism-Spectrum Disorders","authors":"V. Senthilraja, E. Yang, A. Reddy, E. Liu, Himanshu Wagh","doi":"10.53043/2320-1991.acb90015","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90015","url":null,"abstract":"Many children with Autism-Spectrum Disorders (ASD) struggle with sleep dysfunction. This can be caused by a lack of important gut microbiota (GM) that have the ability to influence functions of the nervous system through the gut-brain axis. The metabolites of GM function are responsible for influencing the production of pertinent sleep hormones, such as melatonin and serotonin. The research has identified lower abundance levels of the gut microbiota species Faecalibacterium and Agathobacter in children with ASD that struggled with sleep disorders. When exploring their impact on sleep hormone production, a positive correlation was identified between these species of GM and melatonin levels, which regulates circadian sleep cycles. In addition, a negative correlation was observed between these species of GM and serotonin levels, which high levels of can lead to wakefulness and sleep dysfunction. To improve GM levels in children with ASD, a Bimuno® galactooligosaccharide (B-GOS®) prebiotic intervention was tested in children with ASD. While this intervention led to improved GM levels and increased levels of Faecalibacterium growth, a significant difference was not noticed between groups. Furthermore, an analysis of probiotic intervention studies in various individuals revealed the ability to improve sleep metric scores through consumption of probiotics. These findings can be further explored in children with ASD for potential treatments for sleep disorders.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127256116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"90.10. MedBed and its Effect on Cultivated Intestinal Epithelial Cells and Functional Neutrophils","authors":"P. Dartsch","doi":"10.53043/2320-1991.acb90016","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90016","url":null,"abstract":"Background: Quantum entanglement is a phenomenon in theoretical physics that happens when pairs or groups of particles are generated in such a way that the quantum state of each particle cannot be described independently of the others even when the particles are separated by a large distance. Material and methods: The 90.10. MedBed is a virtual bed that is quantum entangled with a 90.10.-CUBE by a quantum processor, the 9010MedBedOS. The 90.10.-CUBE was located in Akumal Quintana Roo, Mexico, 8,603 kilometers air-line distance from the cell culture laboratory conducting the tests. The 90.10. MedBed is a virtual bed that is quantum entangled with a 90.10.-CUBE by a quantum processor, the 9010MedBedOS. By the process of 90.10. quantum entanglement, the 90.10. CUBE’s basic potential is transferred to the 90.10. MedBed. For the present in vitro-study with intestinal epithelial cells and functional neutrophils – at laboratory conditions instead of a bed – seeded cells in the culture dishes were quantum entangled with the 90.10. MedBed, i.e. the system was installed on the dishes and activated in the laboratory to conduct the study. Control dishes were not treated and were incubated in the same incubator with a distance of at least 30 to 40 cm to the treated dishes. Results: For intestinal epithelial cells, a better regeneration process was determined demonstrating that 90.10. MedBed was able to promote healing processes of the intestinal cell layer and, thus, to promote intestinal and systemic health and wellbeing. The second experimental series with functional neutrophils as inflammationmediating cells demonstrated a marked decrease in the generation of reactive superoxide anion radicals by exposure to 90.10. MedBed quantum entanglement. In vivo, this influence might have a positive impact on the healing process of complicated secondary wounds or on (chronic) inflammatory processes directly. Conclusions: The results clearly demonstrate the effectiveness of the 90.10. MedBed which was quantum entangled with a 90.10.-CUBE by a quantum processor, the 9010MedBedOS, on the cellular level. The results confirm and complement previous findings on the effect of 90.10. quantum entanglement on cultured organ-specific cells in which we demonstrated an increased regeneration of cultured connective tissue fibroblasts.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129196922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}