Applied Cell Biology最新文献

{"title":"Protective Effect of the QiBracelet® Against Oxidative Stress","authors":"Peter C. Dartsch","doi":"10.53043/2320-1991.acb12001","DOIUrl":"https://doi.org/10.53043/2320-1991.acb12001","url":null,"abstract":"Environmental oxidative stress is caused by different artificial and natural sources (environmental stressors). These influences might increase the generation of reactive oxygen species which are known to cause unwanted adverse health effects. According to the manufacturer, the QiBracelet® contains a grid chip which forms a static field that stimulates water molecules to undergo a transition into the coherent state. Since our body consists of about 70-85 % of water, the coherent state of the water molecules should be able to influence the cells of our whole body in a positive manner. Thus, the cells might obtain the ability to compensate any environmental stressors causing oxidative stress much more efficient when compared with endogenous enzymatic systems. The goal of this study was to investigate whether different cultured cell types were able to resist exogenous oxidative stress by addition of 2 mM hydrogen peroxide to the culture medium much more efficient in the presence of the QiBracelet® in comparison to unprotected control cells. Without protection, connective tissue fibroblasts (L-929) were the most sensitive cell type, while lung cells (A-549) were the least sensitive; connective tissue fibroblasts showed a loss in viability of 24% and lung cells of 84%. The other cell types such as kidney cells (MDCK), liver cells (Hep G2) and intestinal epithelial cells (IPEC-J2) had a viability between the two values. Independent from cell type, the QiBracelet® demonstrated a protective effect against exogenous oxidative stress. The extent of this protection varied among cell types, with liver cells showing the highest level of protection at 47.3 ± 7.1 %, followed by connective tissue fibroblasts at 29.6 ± 5.6%, kidney cells at 27.1 ± 5.9%, intestinal epithelial cells at 18.0 ± 7.1%, and lung cells at only 3.9 ± 2.8%. The results demonstrate that the use of the QiBracelet® was able to protect all tested cell types from the hydrogen peroxide-induced environmental oxidative stress. Therefore, the QiBracelet® might also promote and maintain a better well-being and attitude of life in vivo.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141826988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial Health Effects of Malic Acid Water Treatment on Cultured Intestinal Epithelial Cells and Functional Neutrophils","authors":"Peter C. Dartsch, Fabio Hüther","doi":"10.53043/2320-1991.acb12004","DOIUrl":"https://doi.org/10.53043/2320-1991.acb12004","url":null,"abstract":"The Swiss company Evodrop has developed a malic acid granulate cartridge technology to reduce calcium carbonate deposition in water pipes and improves the quality of drinking water. Cell biological methods were used to investigate whether tap water, after passing through the filter system with the malic acid granulate cartridge, has additional beneficial effects in comparison to the original tap water. By using cultured intestinal epithelial cells, we examined the regeneration and the intestinal epithelial barrier integrity as well as the anti-inflammatory potential of this water treatment. The regeneration of the intestinal epithelial cells was significantly stimulated by the malic acid filter cartridge treatment when compared to the initial tap water. The residual cell-free area was 17.3 ± 1.7% (mean ± standard deviation) for the untreated tap water and only 10.5 ± 3.0% (mean ± standard deviation) for the tap water after malic acid filter cartridge treatment. This difference in colonized area was statistically significant (p ≤ 0.05). In addition, the cell layers incubated with the untreated tap water had a transepithelial electrical resistance (TEER) of 1,522 ± 111 Ω/cm2 (mean ± standard deviation) and the cells incubated with the tap water after malic acid filter cartridge treatment had a TEER of 4,183 ± 495 Ω/cm2 (mean ± standard deviation). This represents an increase in intestinal epithelial barrier integrity by more than 2.5-fold and was statistically highly significant (p ≤ 0.01). Both water samples caused a concentration-dependent reduction in endogenous radical formation during an oxidative burst of functional neutrophils as inflammatory-mediating cells. However, the reduction of radical formation, and thus also the anti-inflammatory effect, was 22 ± 4.7% (mean ± standard deviation; p ≤ 0.01) better with the tap water after malic acid filter cartridge treatment than with the untreated tap water at a concentration of 40 vol%. Even at 10 vol%, the tap water after malic acid filter cartridge treatment reduced radical formation by 15.2 ± 6.6% (mean ± standard deviation; p ≤ 0.05) when compared with the untreated tap water. The cell culture tests performed in this study proved that tap water, after passing through the filter system with the malic acid granulate cartridge from Evodrop AG, achieved a considerable improvement in quality. This was demonstrated in intestinal epithelial cells by the significant increase in cellular regeneration and integrity of the intestinal epithelial barrier and by the reduction in radical formation of inflammation-mediating cells. Therefore, the malic acid water treatment can contribute to maintain and improve intestinal health and human well-being.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":" 48","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141825497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmed Cell Death Vs Death by Suicide; A Functional Comparison","authors":"Prasanna N. de Silva","doi":"10.53043/2320-1991.acb12003","DOIUrl":"https://doi.org/10.53043/2320-1991.acb12003","url":null,"abstract":"This article reviews the physiology of programmed cell death (PCD); commonly known as cell suicide. The 3 main processes of PCD; apoptosis, necroptosis and pyroptosis are described, including external and internal signalling precipitating cell death. These are then compared with behavioural phenotypes of planned suicide, associated with toxic relationships, ‘anomie’ (disconnection from the contemporary social network), ‘learned helplessness’ (the main model for depression), social media reporting of suicides and political indoctrination leading to suicide bombings. Ethical arguments for and against active medical assistance in completing suicide by people with progressive neuropsychiatric disorders (such as dementias) and those with long standing severe mental illness (such as anorexia and personality disorder) are discussed. Emerging therapeutic prospects of ‘stalling’ PCD and using inflamatory interleukin patterns to predict overwhelming PCD and dramatic suicides are highlighted.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":" 48","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141825384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration Plate 3.0 – Improvement and Maintenance of Intestinal Health by Reduction of Oxidative Stress and Inflammation","authors":"Peter C. Dartsch","doi":"10.53043/2320-1991.acb11008","DOIUrl":"https://doi.org/10.53043/2320-1991.acb11008","url":null,"abstract":"Background: Sleep is an integral biological necessity and is understood to possess recuperative and regenerative properties. Sleep deprivation has been associated with diseases and an increase in morbidity and mortality. Several recent studies have suggested a strong relation between insufficient sleep and gastrointestinal diseases, especially when triggered by inflammatory processes. Accordingly to these findings it has been reported that sleep deprivation in both humans and experimental animals causes a progressive increase in circulating white blood cells, mainly neutrophils as well as an increase in various circulating proinflammatory molecules. Experimental: Against this background we used cultured intestinal epithelial cells to investigate the positive impact of a specially designed device, Regeneration Plate 3.0, which is positioned under the bed during sleep and is stated to improve systemic health on the cellular level by reducing oxidative stress which acts on the body. The plate produces a vital field with a frequency pool containing all important regeneration frequencies within a radius of 90 cm. The body's own energy field only resonates with those frequencies that are required for an optimal supply of energy to the cells. The field strength of the vital field is adjusted in such a way that the energy system cannot be over-energized. In addition, we also used an in vitro model with inflammation-mediating cells (= functional neutrophils) to examine whether the Regeneration Plate 3.0 might be able to reduce the generation of reactive oxygen radicals during an inflammatory process. Results: The results demonstrate that the Regeneration Plate 3.0 was able to reduce oxidative stress acting on intestinal epithelial cells. After 24 hours, the percentage of surviving cells after exposure to 2 mM hydrogen peroxide and the Regeneration Plate 3.0 for 8 hours was 27.7 ± 5.7%, while the viability of the untreated control cells was 14.7 ± 2.9% (mean values ± standard deviations). The difference between both experimental groups was statistically highly significant at the p ≤ 0.01 level. Moreover, the generation of superoxide anion radicals by functional neutrophils was reduced by nearly 50% in comparison to untreated control cells. Again, the difference between both groups was statistically highly significant at the p ≤ 0.01 level. Conclusions: Both beneficial effects of the Regeneration Plate 3.0 shown in the present in vitro study can act on the body during sleep and might enhance not only intestinal health, but also systemic health and well-being.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136295018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commentary: Loss of FOCAD, Operating via the SKI Messenger RNA Surveillance Pathway, Causes a Pediatric Syndrome with Liver Cirrhosis [1]","authors":"R. Traspas","doi":"10.53043/2320-1991.acb11006","DOIUrl":"https://doi.org/10.53043/2320-1991.acb11006","url":null,"abstract":"Liver disease is becoming a major health concern and is estimated to be the fifth most common cause of death worldwide [2]. A systematic review from the Global Burden of Disease Study identified 1.32 million deaths due to liver cirrhosis in 2017, accounting for more than two percent of the total global deaths [3]. Liver cirrhosis is usually diagnosed late in life; it is irreversible, staying chronic for the rest of the patient ́s life; and it can be life-threatening, as it may evolve into hepatocellular carcinoma or lead to liver failure. Despite extensive research, to date none of the tested compounds for treating this disorder have proven to be safe and effective in human patients. Current available therapies are generally palliative and focused on slowing down the disease, with liver transplant remaining as the gold standard strategy to treat patients with decompensated cirrhosis. Therefore, there is a patent need for the identification and validation of new targets to treat, reverse and, ultimately, cure this hepatic disorder.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132389950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial Effect of Sustainable Magnetic Treatment of Tap Water Investigations with Cultivated Intestinal Epithelial Cells","authors":"P. Dartsch","doi":"10.53043/2320-1991.acb11007","DOIUrl":"https://doi.org/10.53043/2320-1991.acb11007","url":null,"abstract":"(Electro)magnetic fields have been used as a tap water treatment since more than 60 years to prevent water pipe walls from the unwanted deposition of calcium carbonate. In the meantime a lot of research has been done on the effects of magnetic or electromagnetic treatment of water. Most of them deal with physiochemical aspects and only a few report on biological effects on prokaryotes or cultured mammalian cells. Intestinal health plays a key role in respect to systemic health and the intestinal epithelial barrier comes directly into contact with drinking water, the effect of magnetic tap water treatment on the integrity of the intestinal epithelial barrier was investigated by measurement of the transepithelial electrical resistance (TEER). Moreover, the sensitivity of the intestinal epithelial barrier against oxidative stress was investigated. Since the regeneration process of intestinal epithelial cells is essential for the reconstitution and maintenance of the barrier integrity after injury or traumatization in vivo, this aspect was also examined with water ± magnetic treatment. The animal cell line IPEC-J2 was used for the investigations. Local tap water (= initial source water) was obtained before and after magnetic treatment with water core magnets which show a very weak field strength with high magnetic gradients as a special characteristic. TEER increased by 28.5 ± 6.8% after incubation with the tap water with magnetic treatment when compared to the initial tap water (mean value ± standard deviation; p ≤ 0.01 by the two-tailed Wilcoxon-Mann-Whitney rank sum test). Measurement of TEER after oxidative stress showed no marked difference between both tap water samples ± magnetic treatment. In both experimental series there was a significant reduction in TEER by more than 90 % representing a leaky intestinal epithelial cell layer due to oxidative stress. Despite this fact the number of cells in the initial tap water sample was largely decreased whereas the cell density in the treated water samples was much higher. The magnetic treatment of the tap water improved the regeneration of the intestinal epithelial cells by 17.4 ± 7.2% (mean value ± standard deviation; p ≤ 0.05 by the two-tailed Wilcoxon-Mann-Whitney rank sum test) when compared with the initial tap water. The results of the study demonstrate that the water core magnets as used here are able to influence tap water and, subsequently, induce beneficial cellular effects on cultured intestinal epithelial cells. Thus, the tap water with magnetic treatment might be able to promote and maintain intestinal and systemic health in vivo.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127156110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Vibration-Based Holistic Approach to Predict Drug-Target Interactions","authors":"Ziwei Zhao, Xuweisheng Ji, Yun Wang","doi":"10.53043/2320-1991.acb11005","DOIUrl":"https://doi.org/10.53043/2320-1991.acb11005","url":null,"abstract":"","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133007027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Usage Anesthesia in the Surgical Treatment of Diabetic Macular Edema","authors":"A. Reddy, Allen Dang, Sarah Yee, Muhammad S. Ghauri, Yui Seo, Neel Nawathey, Alex Bachir, Telak Brahmbhatt, Rakesh Patel","doi":"10.53043/2320-1991.acb11003","DOIUrl":"https://doi.org/10.53043/2320-1991.acb11003","url":null,"abstract":"Diabetes-related macular edema is a fast-spreading condition that has damaged the lives of countless people throughout the globe. The current incorporation of subthreshold micropulse laser has indicated the precedence of laser therapy in the treatment of diabetic macular edema; nonetheless, vitreoretinal surgery in conjunction with anesthetics correlates with early mitigation of damage to the fovea. Surgery is a delicate procedure that necessitates careful consideration of the types and locations of anesthetics used. Depending on the individual being treated, various drugs and methods may be necessary. For example, some individuals may be allergic to conventional anesthetics, resulting in more severe consequences. In the surgical treatment of diabetic macular edema, the topical anesthetic was utilized more frequently than subconjunctival anesthesia, according to the review’s statistical analysis. The examined literature indicated that topical lidocaine was the most employed anesthetic for vitreoretinal surgery; however, alternatives to lidocaine exist, and their effects should be investigated. Consequently, we would want to highlight the impacts of each of the several surgical medications and anesthetics used to treat diabetic macular edema.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115310170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medical Implications of Marijuana and Cannabis-Derived Products","authors":"Arjavon T Talebzadeh","doi":"10.53043/2320-1991.acb11004","DOIUrl":"https://doi.org/10.53043/2320-1991.acb11004","url":null,"abstract":"This study was conducted to better understand the medical implications of marijuana and the biochemical basis of its effects on psychiatric health. Δ9-tetradydrocannabinol (THC) is a cannabinoid in marijuana with structure similar to neurotransmitter anandamide, allowing THC to bind to cannabinoid receptors on regions of the nervous system associated with memory formation, coordination, and attention focus. THC and CBD in marijuana are observed to have implications for treatment in cancer patients for moderate pain relief, narcoleptic seizures, and possibly inhibition of cancer cell activity. Regulation of marijuana should be considered due to its great potential for misuse. This study will review will reflect upon the biochemical properties of marijuana and other products derived from Cannabis and methods of marijuana ingestion for both recreational and medical purposes. It will further elaborate upon the implications of Cannabis in medical use, side effects, social risks and the holistic overview of the basic properties of Cannabis and other implications.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114233253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LDL Apheresis And/or Human Monoclonal Antibodies as Treatment in Se-vere Dyslipoproteinemia","authors":"olf Bambauer, R. Schiel","doi":"10.53043/2320-1991.acb11002","DOIUrl":"https://doi.org/10.53043/2320-1991.acb11002","url":null,"abstract":"","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132324081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}