{"title":"Rapid Label-free Nanotechnological Immunoassay for Analysis of Candidate Malaria Vaccines","authors":"G. Brunetti, Annalisa De Pastina, M. Hegner","doi":"10.53043/2320-1991.acb90006","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90006","url":null,"abstract":"We recently demonstrated a direct single-step label-free quantitative immunoassay in serum, investigating malaria vaccines [1]. Our method exhibits 1 pg mass sensitivity at concentrations of approx. 100pg/ml, on par with the goldstandard multi-step enzyme-linked immunosorbent assay (ELISA). The nanomechanical assays perform faster and enable simultaneous mass uptake studies of multiple targets, due to epitope-specific recognition [1]. In nanomechanical assays parallel measurement points are represented over time (5s time resolution), thus providing additional temporal information compared with the ELISA output of averaged single end-point measurements. This commentary elucidates the additional advantages of label-free differential assays over the classical immunosorbent assay.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115391372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathaniel Tak, Pranshu Wagh, Sumer Sandhu, A. Reddy, Himanshu Wagh
{"title":"A Quantitative Analysis on the Effect of Varying Nitrate Concentrations on pH levels on the Growth of Algae","authors":"Nathaniel Tak, Pranshu Wagh, Sumer Sandhu, A. Reddy, Himanshu Wagh","doi":"10.53043/2320-1991.acb90005","DOIUrl":"https://doi.org/10.53043/2320-1991.acb90005","url":null,"abstract":"The objective of this experiment was to determine what nitrate concentration and pH would provide the most optimal growth for algae. In order to determine this, algae from the American River was taken and grown in solutions with different concentrations of nitrate, and H+ ions. It was hypothesized that the algae would grow best in solutions with neutral pH levels or solutions with high nitrate concentrations. Different amounts of sodium nitrate were added to beakers with similar amounts of water and algae in order to create environments with varying concentrations of nitrate. Different amounts of NaOH and acetic acid were added to beakers with similar amounts of water and algae in order to simulate environments with varying pH levels. This experiment was conducted in order to determine how fertilizer runoffs affect algal growth. Fertilizer run offs carry nitrate ions into rivers and lakes, which cause algal blooms to form. It was hypothesized that as the concentration of nitrate increases, the growth rate of the algae would also increase. This is due to the fact that the literature supports the idea that as nitrate is added to rivers and lakes the number of algae present increases. The results from the experiment demonstrated that the most optimal concentration of nitrate in the water for algal growth was in between 1-2 Molar and that the most optimal pH for algal growth was in between 7-8.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125496637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Comparison of DASH Scores Resulting from Different Treatment Options for the Intra-articular Distal Radius Fracture in the Geriatric Population","authors":"Himanshu Wagh, A. Reddy","doi":"10.53043/2320-1991.ACB90004","DOIUrl":"https://doi.org/10.53043/2320-1991.ACB90004","url":null,"abstract":"There exist three main methods of treating distal radius fractures: closed reduction, open reduction internal fixation, and percutaneous fixation. 10 studies in the geriatric population comparing closed reduction and ORIF, and ORIF and methods of percutaneous treatment were found. DASH and PRWE scores from these studies at 1 year followup were compiled and means were compared via independent T tests. DASH scores in the ORIF and percutaneous treatment groups were better than the open reduction, although ORIF and percutaneous treatment groups were not significantly different. PRWE score analysis revealed a significant advantage of ORIF compared to percutaneous treatment, and percutaneous treatment over closed reduction and casting.","PeriodicalId":191002,"journal":{"name":"Applied Cell Biology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115255875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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