Nature biotechnology最新文献_第5页

Toward a safer and more secure US bioeconomy 朝着更安全、更有保障的美国生物经济迈进

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-12-16 DOI: 10.1038/s41587-024-02519-2

Matthew C. Watson, Kunal J. Rambhia, Meghan J. Seltzer, Sarah R. Carter, Rebecca L. Moritz, Aurelia Attal-Juncqua, James Diggans, John Dileo

引用次数: 0

The devolution of biosimilars regulations 生物仿制药监管的权力下放

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-12-16 DOI: 10.1038/s41587-024-02497-5

Erik Doevendans, Peter van Meer, Huub Schellekens

引用次数: 0

A general system for targeting MHC class II–antigen complex via a single adaptable loop 一种通过单一适应性环靶向MHC ii类抗原复合物的通用系统

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-12-13 DOI: 10.1038/s41587-024-02466-y

Haotian Du, Jingjia Liu, Kevin M. Jude, Xinbo Yang, Ying Li, Braxton Bell, Hongli Yang, Audrey Kassardjian, Wyatt Blackson, Ali Mobedi, Udit Parekh, R. Andres Parra Sperberg, Jean-Philippe Julien, Elizabeth D. Mellins, K. Christopher Garcia, Po-Ssu Huang

{"title":"A general system for targeting MHC class II–antigen complex via a single adaptable loop","authors":"Haotian Du, Jingjia Liu, Kevin M. Jude, Xinbo Yang, Ying Li, Braxton Bell, Hongli Yang, Audrey Kassardjian, Wyatt Blackson, Ali Mobedi, Udit Parekh, R. Andres Parra Sperberg, Jean-Philippe Julien, Elizabeth D. Mellins, K. Christopher Garcia, Po-Ssu Huang","doi":"10.1038/s41587-024-02466-y","DOIUrl":"https://doi.org/10.1038/s41587-024-02466-y","url":null,"abstract":"Major histocompatibility complex class II (MHCII) bound to a peptide antigen mediates interactions between CD4+ T cells and antigen-presenting cells. Targeting peptide–MHCII with T cell antigen receptors (TCRs) and TCR-like antibodies has shown promise for autoimmune diseases and microbiome tolerance. To develop a general targeting approach, we introduce targeted recognition of antigen–MHC complex reporter for MHCII (TRACeR-II) for the rapid development of peptide-specific MHCII binders. TRACeR-II binders have a small helical bundle scaffold and use a single loop to recognize peptide–MHCII, which offers versatility and enables structural modeling of the interactions to target MHCII antigens. We demonstrate rapid generation of TRACeR-II binders to multiple molecules with affinities in the low-nanomolar to low-micromolar range, comparable to best-in-class TCRs and antibodies. Through computational protein design, we created specific binding sequences in silico from only the sequence of a severe acute respiratory syndrome coronavirus 2 peptide. TRACeR-II provides a straightforward approach to target antigen–MHCII without relying on combinatorial selection on complementarity-determining region loops.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"352 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biopharma deals get smaller and earlier 生物制药的交易越来越小，越来越早

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-12-13 DOI: 10.1038/s41587-024-02506-7

Melanie Senior

引用次数: 0

A synthetic scaffold to target peptide–MHC complexes 靶向肽- mhc复合物的合成支架

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-12-13 DOI: 10.1038/s41587-024-02513-8

Pallavi A. Balivada, Stephanie A. Gaglione, Michael E. Birnbaum

引用次数: 0

Targeting peptide antigens using a multiallelic MHC I-binding system 利用多等位基因MHC i结合系统靶向肽抗原

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-12-13 DOI: 10.1038/s41587-024-02505-8

Haotian Du, Leena Mallik, Daniel Hwang, Yi Sun, Chengzi Kaku, Daniel Hoces, Shirley M. Sun, Reem Ghinnagow, Stephen D. Carro, Hoang Anh T. Phan, Sagar Gupta, Wyatt Blackson, Hyejin Lee, Christian A. Choe, Devin Dersh, Jingjia Liu, Braxton Bell, Hongli Yang, Georgia F. Papadaki, Michael C. Young, Emily Zhou, Gina El Nesr, Kimia Dasteh Goli, Laurence C. Eisenlohr, Andy J. Minn, Rogelio A. Hernandez-Lopez, Joseph G. Jardine, Nikolaos G. Sgourakis, Po-Ssu Huang

{"title":"Targeting peptide antigens using a multiallelic MHC I-binding system","authors":"Haotian Du, Leena Mallik, Daniel Hwang, Yi Sun, Chengzi Kaku, Daniel Hoces, Shirley M. Sun, Reem Ghinnagow, Stephen D. Carro, Hoang Anh T. Phan, Sagar Gupta, Wyatt Blackson, Hyejin Lee, Christian A. Choe, Devin Dersh, Jingjia Liu, Braxton Bell, Hongli Yang, Georgia F. Papadaki, Michael C. Young, Emily Zhou, Gina El Nesr, Kimia Dasteh Goli, Laurence C. Eisenlohr, Andy J. Minn, Rogelio A. Hernandez-Lopez, Joseph G. Jardine, Nikolaos G. Sgourakis, Po-Ssu Huang","doi":"10.1038/s41587-024-02505-8","DOIUrl":"https://doi.org/10.1038/s41587-024-02505-8","url":null,"abstract":"Identifying highly specific T cell receptors (TCRs) or antibodies against epitopic peptides presented by class I major histocompatibility complex (MHC I) proteins remains a bottleneck in the development of targeted therapeutics. Here, we introduce targeted recognition of antigen–MHC complex reporter for MHC I (TRACeR-I), a generalizable platform for targeting peptides on polymorphic HLA-A*, HLA-B* and HLA-C* allotypes while overcoming the cross-reactivity challenges of TCRs. Our TRACeR–MHC I co-crystal structure reveals a unique antigen recognition mechanism, with TRACeR forming extensive contacts across the entire peptide length to confer single-residue specificity at the accessible positions. We demonstrate rapid screening of TRACeR-I against a panel of disease-relevant HLAs with peptides derived from human viruses (human immunodeficiency virus, Epstein–Barr virus and severe acute respiratory syndrome coronavirus 2), and oncoproteins (Kirsten rat sarcoma virus, paired-like homeobox 2b and New York esophageal squamous cell carcinoma 1). TRACeR-based bispecific T cell engagers and chimeric antigen receptor T cells exhibit on-target killing of tumor cells with high efficacy in the low nanomolar range. Our platform empowers the development of broadly applicable MHC I-targeting molecules for research, diagnostic and therapeutic applications.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"15 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BioNTech boosts oncology pipeline with China buy BioNTech在中国收购肿瘤学产品线

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-12-11 DOI: 10.1038/s41587-024-02518-3

引用次数: 0

Efficient non-viral immune cell engineering using circular single-stranded DNA-mediated genomic integration 利用环状单链dna介导的基因组整合进行高效的非病毒免疫细胞工程

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2024-12-11 DOI: 10.1038/s41587-024-02504-9

Keqiang Xie, Jakob Starzyk, Ishita Majumdar, Jiao Wang, Katerina Rincones, Thao Tran, Danna Lee, Sarah Niemi, John Famiglietti, Bernhard Suter, Richard Shan, Hao Wu

{"title":"Efficient non-viral immune cell engineering using circular single-stranded DNA-mediated genomic integration","authors":"Keqiang Xie, Jakob Starzyk, Ishita Majumdar, Jiao Wang, Katerina Rincones, Thao Tran, Danna Lee, Sarah Niemi, John Famiglietti, Bernhard Suter, Richard Shan, Hao Wu","doi":"10.1038/s41587-024-02504-9","DOIUrl":"https://doi.org/10.1038/s41587-024-02504-9","url":null,"abstract":"The use of adeno-associated viruses (AAVs) as donors for homology-directed repair (HDR)-mediated genome engineering is limited by safety issues, manufacturing constraints and restricted packaging limits. Non-viral targeted genetic knock-ins rely primarily on double-stranded DNA (dsDNA) and linear single-stranded DNA (lssDNA) donors. dsDNA is known to have low efficiency and high cytotoxicity, while lssDNA is challenging for scaled manufacture. In this study, we developed a non-viral genome writing catalyst (GATALYST) system that allows production of circular single-stranded DNAs (cssDNAs) up to approximately 20 kilobases as donor templates for highly efficient precision transgene integration. cssDNA donors enable knock-in efficiency of up to 70% in induced pluripotent stem cells (iPSCs) and improved efficiency in multiple clinically relevant primary immune cell types and at multiple genomic loci implicated for clinical applications with various nuclease editor systems. The high precision and efficiency in chimeric antigen receptor (CAR)-T and natural killer (NK) cells, improved safety, payload flexibility and scalable manufacturability of cssDNA shows potential for future applications of genome engineering.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"25 6 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A technical approach to global plant genome editing regulation 全球植物基因组编辑调控的技术途径

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-12-11 DOI: 10.1038/s41587-024-02489-5

Evan Groover, Elizabeth Njuguna, Kailash Chander Bansal, Anne Muia, Musa Kwehangana, Christopher Simuntala, Richard Lloyd Mills, Emmanuel Kwakye, Pedro Rocha, Josephine Amedu, Eduardo Morillo, Mohana Anita Anthonysamy, A. B. M. Khaldun, Lilian Chimpepo, Massouroudini Akoudjin, D. M. J. B. Senanayake, Dechen Wangmo, Dessalegn Atnafu, Geronima P. Eusebio, Chalinee Kongsawat, Melinda Kliegman

引用次数: 0

Intellectual property training should be embedded in the biomedical education process 知识产权培训应纳入生物医学教育过程

IF 33.1 1区生物学

Nature biotechnology Pub Date : 2024-12-11 DOI: 10.1038/s41587-024-02499-3

Randal A. Serafini, Micaila D. E. Curtis, Stella Alimperti

引用次数: 0