以单细胞分辨率进行不可知病毒检测，揭示新型病毒

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2025-04-24 DOI:10.1038/s41587-025-02638-4

{"title":"以单细胞分辨率进行不可知病毒检测，揭示新型病毒","authors":"","doi":"10.1038/s41587-025-02638-4","DOIUrl":null,"url":null,"abstract":"We introduce a method that detects viral sequences in RNA sequencing data on the basis of highly conserved proteins, enabling the detection of more than 100,000 RNA virus species. We analyzed the presence of novel viruses and host gene expression in parallel to characterize viral tropism and host immune responses in individual cells.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"35 1","pages":""},"PeriodicalIF":33.1000,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Agnostic viral detection at single-cell resolution reveals novel viruses\",\"authors\":\"\",\"doi\":\"10.1038/s41587-025-02638-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We introduce a method that detects viral sequences in RNA sequencing data on the basis of highly conserved proteins, enabling the detection of more than 100,000 RNA virus species. We analyzed the presence of novel viruses and host gene expression in parallel to characterize viral tropism and host immune responses in individual cells.\",\"PeriodicalId\":19084,\"journal\":{\"name\":\"Nature biotechnology\",\"volume\":\"35 1\",\"pages\":\"\"},\"PeriodicalIF\":33.1000,\"publicationDate\":\"2025-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nature biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1038/s41587-025-02638-4\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1038/s41587-025-02638-4","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

我们介绍了一种基于高度保守蛋白在RNA测序数据中检测病毒序列的方法，能够检测超过10万种RNA病毒。我们分析了新病毒和宿主基因表达的平行存在，以表征单个细胞中的病毒趋向性和宿主免疫反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Agnostic viral detection at single-cell resolution reveals novel viruses

查看原文本刊更多论文

Agnostic viral detection at single-cell resolution reveals novel viruses

We introduce a method that detects viral sequences in RNA sequencing data on the basis of highly conserved proteins, enabling the detection of more than 100,000 RNA virus species. We analyzed the presence of novel viruses and host gene expression in parallel to characterize viral tropism and host immune responses in individual cells.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

期刊介绍： Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research. The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field. Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology. In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.