William N. Feist, Sofia E. Luna, Kaya Ben-Efraim, Maria V. Filsinger Interrante, Alvaro Amorin, Nicole M. Johnston, Theodora U. J. Bruun, Ashley Utz, Hana Y. Ghanim, Benjamin J. Lesch, Theresa M. McLaughlin, Amanda M. Dudek, Matthew H. Porteus
{"title":"Multilayered HIV-1 resistance in HSPCs through CCR5 Knockout and B cell secretion of HIV-inhibiting antibodies","authors":"William N. Feist, Sofia E. Luna, Kaya Ben-Efraim, Maria V. Filsinger Interrante, Alvaro Amorin, Nicole M. Johnston, Theodora U. J. Bruun, Ashley Utz, Hana Y. Ghanim, Benjamin J. Lesch, Theresa M. McLaughlin, Amanda M. Dudek, Matthew H. Porteus","doi":"10.1038/s41467-025-58371-8","DOIUrl":"https://doi.org/10.1038/s41467-025-58371-8","url":null,"abstract":"<p>Allogeneic transplantation of <i>CCR5</i> null hematopoietic stem and progenitor cells (HSPCs) is the only known cure for HIV-1 infection. However, this treatment is limited because of the rarity of <i>CCR5</i>-null matched donors, the morbidities associated with allogeneic transplantation, and the prevalence of HIV-1 strains resistant to <i>CCR5</i> knockout (KO) alone. Here, we propose a one-time therapy through autologous transplantation of HSPCs genetically engineered ex vivo to produce both <i>CCR5</i> KO cells and long-term secretion of potent HIV-1 inhibiting antibodies from B cell progeny. CRISPR-Cas9-engineered HSPCs engraft and reconstitute multiple hematopoietic lineages in vivo and can be engineered to express multiple antibodies simultaneously (in pre-clinical models). Human B cells engineered to express each antibody secrete neutralizing concentrations capable of inhibiting HIV-1 pseudovirus infection in vitro. This work lays the foundation for a potential one-time functional cure for HIV-1 through combining the long-term delivery of therapeutic antibodies against HIV-1 and the known efficacy of <i>CCR5</i> KO HSPC transplantation.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"183 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Lin, Hui Li, Xiaodong Miao, Yunayuan Sun, Hao Ren, Xifeng Yu, Wangyang Cui, Mingbo Wu, Zhongtao Li
{"title":"V activated electro-epoxidation catalyst in membrane electrode assembly system for the production of propylene oxide","authors":"Yan Lin, Hui Li, Xiaodong Miao, Yunayuan Sun, Hao Ren, Xifeng Yu, Wangyang Cui, Mingbo Wu, Zhongtao Li","doi":"10.1038/s41467-025-58486-y","DOIUrl":"https://doi.org/10.1038/s41467-025-58486-y","url":null,"abstract":"<p>Direct electro-epoxidation of propylene (D-EOPO) with a membrane electrode assembly (MEA) system represents a sustainable approach for producing propylene oxide, which can reduce ohmic losses and simplify product separation. To address the challenges of selectivity and activity, we develop an Ag/V catalyst and integrate it into the “liquid-free” MEA reactor for continues D-EOPO. The V in the catalyst facilitates the formation of Ag-O active centers, thereby reducing the generation energy of *O radicals. Meanwhile, V doping also results in a downshift of the <i>d</i>-band center of the Ag sites. Consequently, the formation of the crucial intermediate (*OC<sub>3</sub>H<sub>6</sub>) is significantly accelerated through the coupling *O with adsorbed propylene, thereby markedly improving propylene oxide (PO) production. The MEA reactor, integrated with the developed Ag/V catalyst, can maintain a stable production rate of PO at 227 μmol/h over a period of 78 hours. Thus, the “liquid-free” electro-epoxidation protocol developed here exhibits greater industrial applicability.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"24 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lysine-arginine imbalance overcomes therapeutic tolerance governed by the transcription factor E3-lysosome axis in glioblastoma","authors":"Yongwei Jing, Masahiko Kobayashi, Mahmoud I. Shoulkamy, Meiqi Zhou, Ha Thi Vu, Hiroshi Arakawa, Hemragul Sabit, Sadahiro Iwabuchi, Cong Quang Vu, Atsuko Kasahara, Masaya Ueno, Yuko Tadokoro, Kenta Kurayoshi, Xi Chen, Yuhang Yan, Satoshi Arai, Shinichi Hashimoto, Tomoyoshi Soga, Tomoki Todo, Mitsutoshi Nakada, Atsushi Hirao","doi":"10.1038/s41467-025-56946-z","DOIUrl":"https://doi.org/10.1038/s41467-025-56946-z","url":null,"abstract":"<p>Recent advances in cancer therapy have underscored the importance of targeting specific metabolic pathways. In this study, we propose a precision nutrition approach aimed at lysosomal function in glioblastoma multiforme (GBM). Using patient-derived GBM cells, we identify lysosomal activity as a unique metabolic biomarker of tumorigenesis, controlling the efficacy of temozolomide (TMZ), a standard GBM therapy. Employing combined analyses of clinical patient samples and xenograft models, we further elucidate the pivotal role of Transcription Factor Binding To IGHM Enhancer 3 (TFE3), a master regulator of lysosomal biogenesis, in modulating malignant properties, particularly TMZ tolerance, by regulating peroxisome proliferator-activated receptor-gamma coactivator 1−alpha (PGC1α)-mediated mitochondrial activity. Notably, we find that lysine protects GBM cells from lysosomal stress by counteracting arginine’s effects on nitric oxide production. The lysine restriction mimetic, homoarginine administration, significantly enhances the efficacy of anticancer therapies through lysosomal dysfunction. This study underscores the critical role of lysosomal function modulated by amino acid metabolism in GBM pathogenesis and treatment.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"103 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying Liu, Shaofeng Deng, Shuang Ren, Rachel Chun-Yee Tam, Siwen Liu, Anna Jinxia Zhang, Kelvin Kai-Wang To, Kwok-Yung Yuen, Honglin Chen, Pui Wang
{"title":"Intranasal influenza virus-vectored vaccine offers protection against clade 2.3.4.4b H5N1 infection in small animal models","authors":"Ying Liu, Shaofeng Deng, Shuang Ren, Rachel Chun-Yee Tam, Siwen Liu, Anna Jinxia Zhang, Kelvin Kai-Wang To, Kwok-Yung Yuen, Honglin Chen, Pui Wang","doi":"10.1038/s41467-025-58504-z","DOIUrl":"https://doi.org/10.1038/s41467-025-58504-z","url":null,"abstract":"<p>The highly pathogenic avian influenza (HPAI) H5N1 virus has been endemic in aquatic birds since 1997, causing outbreaks in domestic poultry and occasional human infections worldwide. Recently, the cross-species transmission of a new reassortant variant from clade 2.3.4.4b of H5N1 to cattle in the US has heightened concerns regarding the expansion of host range and potential human infection. As eradicating the H5N1 virus from its reservoir is impossible, it is essential to prepare for a potential pandemic caused by an H5N1 derivative. Utilizing a deleted-NS1 live attenuated influenza viral vector vaccine system (DelNS1 LAIV), a system we have previously used in the development of a COVID-19 vaccine, we have rapidly developed an intranasal vaccine for cattle H5N1 and related clade 2.3.4.4b strains, based on publicly available sequences. Our research demonstrates that a single intranasal immunization can provide effective protection against lethal challenges from HPAI cattle or mink H5N1 variants, offering strong, sustained immunity after two months in female mouse and male hamster models. Immunogenicity analysis reveals that intranasal vaccination with DelNS1 LAIV induces robust neutralizing antibody, mucosal IgA and T cell responses in mice. It is crucial to further evaluate the DelNS1-H5N1 LAIV system to prepare for potential future H5N1 outbreaks in humans.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"34 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alejandro Acosta, Wondmagegn Tirkaso, Francesco Nicolli, Thomas P. Van Boeckel, Giuseppina Cinardi, Junxia Song
{"title":"The future of antibiotic use in livestock","authors":"Alejandro Acosta, Wondmagegn Tirkaso, Francesco Nicolli, Thomas P. Van Boeckel, Giuseppina Cinardi, Junxia Song","doi":"10.1038/s41467-025-56825-7","DOIUrl":"https://doi.org/10.1038/s41467-025-56825-7","url":null,"abstract":"<p>Governments worldwide have pledged to reduce antimicrobial use in the agri-food system. This study projects global livestock antibiotic use quantities through 2040 under various scenarios. This work indicates that under a business-as-usual scenario, global antibiotic use could reach ~143,481 tons by 2040, representing a 29.5% increase from the 2019 baseline of ~110,777 tons. However, alternative scenarios suggest that these projections could vary by +14.2% to -56.8%, depending on changes in livestock biomass and antibiotic use intensity. A key contribution of this research is the development of the Livestock Biomass Conversion method, a novel indicator offering improved accuracy in estimating livestock biomass. The findings have important policy implications, highlighting that meaningful reductions in antibiotic use quantity can only be achieved through coordinated efforts targeting both antibiotic use intensity and livestock biomass.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"53 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Somatic NAP1L1 p.D349E promotes cardiac hypertrophy through cGAS-STING-IFN signaling","authors":"Cheng Lv, Xiayidan Alimu, Xiao Xiao, Fei Wang, Jizheng Wang, Shuiyun Wang, Guixin Wu, Yu Zhang, Yue Wu, Houzao Chen, Rutai Hui, Lei Song, Yibo Wang","doi":"10.1038/s41467-025-58453-7","DOIUrl":"https://doi.org/10.1038/s41467-025-58453-7","url":null,"abstract":"<p>Hypertrophic cardiomyopathy (HCM) is the most common inherited heart disease, often caused by sarcomere gene mutations, though many sporadic cases remain genetically unexplained. Here we show that the somatic variant NAP1L1 p.D349E was involved in cardiac hypertrophy in sporadic HCM patients. Through next generation sequencing, we found that somatic variant NAP1L1 p.D349E was recurrent in the cardiomyocytes of gene-elusive sporadic HCM patients. Subsequent in vivo and in vitro functional analysis confirmed that NAP1L1 p.D349E contributes to HCM by triggering an innate immunity response. This mutation destabilizes nucleosome formation, causing DNA to leak into the cytoplasm. This leakage activates a key immune pathway, cGAS-STING, which leads to the release of inflammatory molecules and promotes heart muscle thickening. Our findings reveal a new mechanism driving HCM and suggest that somatic variants could be important in understanding and management of HCM.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"183 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haineng Xu, Erin George, David Gallo, Sergey Medvedev, Xiaolei Wang, Arindam Datta, Rosie Kryczka, Marc L. Hyer, Jimmy Fourtounis, Rino Stocco, Elia Aguado-Fraile, Adam Petrone, Shou Yun Yin, Ariya Shiwram, Fang Liu, Matthew Anderson, Hyoung Kim, Roger A. Greenberg, C. Gary Marshall, Fiona Simpkins
{"title":"Targeting CCNE1 amplified ovarian and endometrial cancers by combined inhibition of PKMYT1 and ATR","authors":"Haineng Xu, Erin George, David Gallo, Sergey Medvedev, Xiaolei Wang, Arindam Datta, Rosie Kryczka, Marc L. Hyer, Jimmy Fourtounis, Rino Stocco, Elia Aguado-Fraile, Adam Petrone, Shou Yun Yin, Ariya Shiwram, Fang Liu, Matthew Anderson, Hyoung Kim, Roger A. Greenberg, C. Gary Marshall, Fiona Simpkins","doi":"10.1038/s41467-025-58183-w","DOIUrl":"https://doi.org/10.1038/s41467-025-58183-w","url":null,"abstract":"<p>Ovarian cancers (OVCAs) and endometrial cancers (EMCAs) with <i>CCNE1-</i>amplification are often resistant to standard treatment and represent an unmet clinical need. Synthetic-lethal screening identified loss of the CDK1 regulator, PKMYT1, as synthetically lethal with <i>CCNE1</i>-amplification. We hypothesize that <i>CCNE1</i>-amplification associated replication stress will be more effectively targeted by combining PKMYT1 inhibitor lunresertib (RP-6306), with ATR inhibitor camonsertib (RP-3500/RG6526). Low dose combination RP-6306 with RP-3500 synergistically increases cytotoxicity more so in <i>CCNE1</i>-amplified compared to non-amplified cells. Combination treatment produces durable antitumor activity, reduces metastasis and increases survival in <i>CCNE1</i>-amplified patient-derived OVCA and EMCA xenografts. Mechanistically, low doses of RP-6306 with RP-3500 increase CDK1 activation more so than monotherapy, triggering rapid and robust induction of premature mitosis, DNA damage, and apoptosis in a <i>CCNE1</i>-dependent manner. These findings suggest that targeting CDK1 activity by combining RP-6306 with RP-3500 is an effective therapeutic approach to treat <i>CCNE1</i>-amplifed OVCAs and EMCAs.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"60 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiazhang Wang, Tianfu Wang, Bingjie Xu, Oliver Cossairt, Florian Willomitzer
{"title":"Accurate eye tracking from dense 3D surface reconstructions using single-shot deflectometry","authors":"Jiazhang Wang, Tianfu Wang, Bingjie Xu, Oliver Cossairt, Florian Willomitzer","doi":"10.1038/s41467-025-56801-1","DOIUrl":"https://doi.org/10.1038/s41467-025-56801-1","url":null,"abstract":"<p>Eye-tracking plays a crucial role in the development of virtual reality devices, neuroscience research, and psychology. Despite its significance in numerous applications, achieving an accurate, robust, and fast eye-tracking solution remains a considerable challenge for current state-of-the-art methods. While existing reflection-based techniques (e.g., “glint tracking\") are considered to be very accurate, their performance is limited by their reliance on sparse 3D surface data acquired solely from the cornea surface. In this paper, we rethink the way how specular reflections can be used for eye tracking: We propose a method for accurate and fast evaluation of the gaze direction that exploits teachings from single-shot phase-measuring-deflectometry. In contrast to state-of-the-art reflection-based methods, our method acquires dense 3D surface information of both cornea and sclera within only one single camera frame (single-shot). For a typical measurement, we acquire >3000× more surface reflection points (\"glints”) than conventional methods. We show the feasibility of our approach with experimentally evaluated gaze errors on a realistic model eye below only 0.13°. Moreover, we demonstrate quantitative measurements on real human eyes in vivo, reaching accuracy values between only 0.46° and 0.97°.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"34 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exercise-induced anti-obesity effects in male mice generated by a FOXO1-KLF10 reinforcing loop promoting adipose lipolysis","authors":"Jie-Ying Zhu, Min Chen, Wang-Jing Mu, Hong-Yang Luo, Yang Li, Shan Li, Lin-Jing Yan, Ruo-Ying Li, Meng-Ting Yin, Xin Li, Hu-Min Chen, Liang Guo","doi":"10.1038/s41467-025-58467-1","DOIUrl":"https://doi.org/10.1038/s41467-025-58467-1","url":null,"abstract":"<p>Exercise combats obesity and metabolic disorders, but the underlying mechanism is incompletely understood. KLF10, a transcription factor involved in various biological processes, has an undefined role in adipose tissue and obesity. Here, we show that exercise facilitates adipocyte-derived KLF10 expression via SIRT1/FOXO1 pathway. Adipocyte-specific knockout of KLF10 blunts exercise-promoted white adipose browning, energy expenditure, fat loss, glucose tolerance in diet-induced obese male mice. Conversely, adipocyte-specific transgenic expression of KLF10 in male mice enhanced the above metabolic profits induced by exercise. Mechanistically, KLF10 interacts with FOXO1 and facilitates the recruitment of KDM4A to form a ternary complex on the promoter regions of <i>Pnpla2</i> and <i>Lipe</i> genes to promote these key lipolytic genes expression by demethylating H3K9me3 on their promoters, which facilitates lipolysis to defend against obesity in male mice. As a downstream effector responding to exercise, adipose KLF10 could act as a potential target in the fight against obesity.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"50 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vincent Rigalleau, Frank Lamy, Nicoletta Ruggieri, Henrik Sadatzki, Helge W. Arz, Stephen Barker, Lester Lembke-Jene, Antje Wegwerth, Gregor Knorr, Igor M. Venancio, Tainã M. L. Pinho, Ralf Tiedemann, Gisela Winckler
{"title":"790,000 years of millennial-scale Cape Horn Current variability and interhemispheric linkages","authors":"Vincent Rigalleau, Frank Lamy, Nicoletta Ruggieri, Henrik Sadatzki, Helge W. Arz, Stephen Barker, Lester Lembke-Jene, Antje Wegwerth, Gregor Knorr, Igor M. Venancio, Tainã M. L. Pinho, Ralf Tiedemann, Gisela Winckler","doi":"10.1038/s41467-025-58458-2","DOIUrl":"https://doi.org/10.1038/s41467-025-58458-2","url":null,"abstract":"<p>Millennial-scale variations in the strength and position of the Antarctic Circumpolar Current exert considerable influence on the global meridional overturning circulation and the ocean carbon cycle. The mechanistic understanding of these variations is still incomplete, partly due to the scarcity of sediment records covering multiple glacial-interglacial cycles with millennial-scale resolution. Here, we present high-resolution current strength and sea surface temperature records covering the past 790,000 years from the Cape Horn Current as part of the subantarctic Antarctic Circumpolar Current system, flowing along the Chilean margin. Both temperature and current velocity data document persistent millennial-scale climate variability throughout the last eight glacial periods with stronger current flow and warmer sea surface temperatures coinciding with Antarctic warm intervals. These Southern Hemisphere changes are linked to North Atlantic millennial-scale climate fluctuations, plausibly involving changes in the Atlantic thermohaline circulation. The variations in the Antarctic Circumpolar Current system are associated with atmospheric CO<sub>2</sub> changes, suggesting a mechanistic link through the Southern Ocean carbon cycle.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"7 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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