Nature metabolism最新文献

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Quantitation of metabolic activity from isotope tracing data using automated methodology 利用自动方法从同位素追踪数据中量化代谢活动
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-25 DOI: 10.1038/s42255-024-01144-2
Shiyu Liu, Xiaojing Liu, Jason W. Locasale
{"title":"Quantitation of metabolic activity from isotope tracing data using automated methodology","authors":"Shiyu Liu, Xiaojing Liu, Jason W. Locasale","doi":"10.1038/s42255-024-01144-2","DOIUrl":"https://doi.org/10.1038/s42255-024-01144-2","url":null,"abstract":"<p>Metabolic flux analysis (MFA) is a computational approach to deciphering labelling patterns based on machine learning principles. Differing from typical machine learning algorithms that train a model from known datasets to make predictions, the commonly used MFA algorithm trains a metabolic network with data from isotope tracing experiments and directly outputs the learned information — that is, all fluxes in the network that best fit data<sup>3,5</sup> (Fig. 1b). However, as a machine learning algorithm, current MFA methods often lack systematic evaluation and benchmarking, a standard practice in broader machine learning and artificial intelligence applications<sup>6</sup>. Issues such as algorithmic convergence, flux estimation accuracy and result robustness in MFA studies have been raised but remain largely unaddressed<sup>3</sup>, limiting the effectiveness and broader adoption of these automated tools in metabolic research.</p><p>To advance the capabilities of MFA for complex metabolic networks and extensive isotope tracing datasets, we developed an automated analysis methodology alongside a large-scale metabolic network model. This model comprises over 100 fluxes across key pathways, including glycolysis, the tricarboxylic acid (TCA) cycle, the pentose phosphate pathway (PPP), one-carbon metabolism, and several amino acid (AA) biosynthetic pathways (Fig. 1c, Supplementary Methods). Compared to contemporary MFA tools<sup>7,8,9</sup>, a notable feature of our methodology is the incorporation of organelle compartmentalization, facilitating accurate quantification of exchange fluxes between mitochondria and cytosol in eukaryotic cells (Fig. 1c). While other tools typically require tens of minutes to obtain a solution<sup>7,9</sup>, our methodology can generate an optimized solution, with fluxes that accurately explain the labelling pattern from a <sup>13</sup>C tracing experiment on cultured cell lines, within 2 s on a desktop computer<sup>10</sup> (Supplementary Fig. 1a–d). Nonetheless, a challenge arose from the observation that these optimized solutions could diverge significantly, showing considerable variability in certain net fluxes even with similar loss values (Fig. 1d, Supplementary Fig. 1e,f).To address this problem, we developed an optimization-averaging algorithm that refines the computation process by selecting a subset of solutions with minimal loss (selected solutions) from the pool of optimized solutions and averaging them to produce a new, more stable solution set (averaged solutions) (Fig. 1e, Supplementary Methods). These solutions, along with those generated using the typical strategy used in contemporary software (Supplementary Methods, Supplementary Fig. 1c), were benchmarked using simulated <sup>13</sup>C tracing datasets generated from a known flux vector (Supplementary Fig. 2a). The results demonstrated that, relative to the benchmark, the optimization-averaging algorithm effectively reduced flux variability and impr","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142489588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Publisher Correction: Exploring pancreatic beta-cell subgroups and their connectivity. 出版商更正:探索胰岛β细胞亚群及其连通性。
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-23 DOI: 10.1038/s42255-024-01141-5
Guy A Rutter,Anne Gresch,Luis Delgadillo Silva,Richard K P Benninger
{"title":"Publisher Correction: Exploring pancreatic beta-cell subgroups and their connectivity.","authors":"Guy A Rutter,Anne Gresch,Luis Delgadillo Silva,Richard K P Benninger","doi":"10.1038/s42255-024-01141-5","DOIUrl":"https://doi.org/10.1038/s42255-024-01141-5","url":null,"abstract":"","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142489502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Farnesyl pyrophosphate potentiates dendritic cell migration in autoimmunity through mitochondrial remodelling 焦磷酸法呢醇酯通过线粒体重塑促进自身免疫中树突状细胞的迁移
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-18 DOI: 10.1038/s42255-024-01149-x
Xiaomin Zhang, Yali Chen, Geng Sun, Yankang Fei, Ha Zhu, Yanfang Liu, Junyan Dan, Chunzhen Li, Xuetao Cao, Juan Liu
{"title":"Farnesyl pyrophosphate potentiates dendritic cell migration in autoimmunity through mitochondrial remodelling","authors":"Xiaomin Zhang, Yali Chen, Geng Sun, Yankang Fei, Ha Zhu, Yanfang Liu, Junyan Dan, Chunzhen Li, Xuetao Cao, Juan Liu","doi":"10.1038/s42255-024-01149-x","DOIUrl":"https://doi.org/10.1038/s42255-024-01149-x","url":null,"abstract":"<p>Cellular metabolism modulates dendritic cell (DC) maturation and activation. Migratory dendritic cells (mig-DCs) travelling from the tissues to draining lymph nodes (dLNs) are critical for instructing adaptive immune responses. However, how lipid metabolites influence mig-DCs in autoimmunity remains elusive. Here, we demonstrate that farnesyl pyrophosphate (FPP), an intermediate of the mevalonate pathway, accumulates in mig-DCs derived from mice with systemic lupus erythematosus (SLE). FPP promotes mig-DC survival and germinal centre responses in the dLNs by coordinating protein geranylgeranylation and mitochondrial remodelling. Mechanistically, FPP-dependent RhoA geranylgeranylation promotes mitochondrial fusion and oxidative respiration through mitochondrial RhoA–MFN interaction, which subsequently facilitates the resolution of endoplasmic reticulum stress in mig-DCs. Simvastatin, a chemical inhibitor of the mevalonate pathway, restores mitochondrial function in mig-DCs and ameliorates systemic pathogenesis in SLE mice. Our study reveals a critical role for FPP in dictating mig-DC survival by reprogramming mitochondrial structure and metabolism, providing new insights into the pathogenesis of DC-dependent autoimmune diseases.</p>","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Farnesyl pyrophosphate modulates dendritic cell migration in lupus autoimmunity 焦磷酸法呢醇酯调节狼疮自身免疫中树突状细胞的迁移
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-18 DOI: 10.1038/s42255-024-01148-y
{"title":"Farnesyl pyrophosphate modulates dendritic cell migration in lupus autoimmunity","authors":"","doi":"10.1038/s42255-024-01148-y","DOIUrl":"https://doi.org/10.1038/s42255-024-01148-y","url":null,"abstract":"Farnesyl pyrophosphate (FPP), an intermediate of cholesterol biosynthesis in the mevalonate pathway, prolongs the survival of migratory dendritic cells (mig-DCs) by remodelling mitochondrial structure and metabolism. Treating a mouse model of systemic lupus erythematosus with simvastatin (an inhibitor of this pathway) led to recovery from dysregulation in mig-DCs and ameliorated systemic autoimmune pathogenesis.","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic architecture of oral glucose-stimulated insulin release provides biological insights into type 2 diabetes aetiology 口服葡萄糖刺激胰岛素释放的基因结构为 2 型糖尿病病因提供了生物学启示
IF 18.9 1区 医学
Nature metabolism Pub Date : 2024-10-17 DOI: 10.1038/s42255-024-01140-6
A. L. Madsen, S. Bonàs-Guarch, S. Gheibi, R. Prasad, J. Vangipurapu, V. Ahuja, L. R. Cataldo, O. Dwivedi, G. Hatem, G. Atla, M. Guindo-Martínez, A. M. Jørgensen, A. E. Jonsson, I. Miguel-Escalada, S. Hassan, A. Linneberg, Tarunveer S. Ahluwalia, T. Drivsholm, O. Pedersen, T. I. A. Sørensen, A. Astrup, D. Witte, P. Damm, T. D. Clausen, E. Mathiesen, T. H. Pers, R. J. F. Loos, L. Hakaste, M. Fex, N. Grarup, T. Tuomi, M. Laakso, H. Mulder, J. Ferrer, T. Hansen
{"title":"Genetic architecture of oral glucose-stimulated insulin release provides biological insights into type 2 diabetes aetiology","authors":"A. L. Madsen,&nbsp;S. Bonàs-Guarch,&nbsp;S. Gheibi,&nbsp;R. Prasad,&nbsp;J. Vangipurapu,&nbsp;V. Ahuja,&nbsp;L. R. Cataldo,&nbsp;O. Dwivedi,&nbsp;G. Hatem,&nbsp;G. Atla,&nbsp;M. Guindo-Martínez,&nbsp;A. M. Jørgensen,&nbsp;A. E. Jonsson,&nbsp;I. Miguel-Escalada,&nbsp;S. Hassan,&nbsp;A. Linneberg,&nbsp;Tarunveer S. Ahluwalia,&nbsp;T. Drivsholm,&nbsp;O. Pedersen,&nbsp;T. I. A. Sørensen,&nbsp;A. Astrup,&nbsp;D. Witte,&nbsp;P. Damm,&nbsp;T. D. Clausen,&nbsp;E. Mathiesen,&nbsp;T. H. Pers,&nbsp;R. J. F. Loos,&nbsp;L. Hakaste,&nbsp;M. Fex,&nbsp;N. Grarup,&nbsp;T. Tuomi,&nbsp;M. Laakso,&nbsp;H. Mulder,&nbsp;J. Ferrer,&nbsp;T. Hansen","doi":"10.1038/s42255-024-01140-6","DOIUrl":"10.1038/s42255-024-01140-6","url":null,"abstract":"The genetics of β-cell function (BCF) offer valuable insights into the aetiology of type 2 diabetes (T2D)1,2. Previous studies have expanded the catalogue of BCF genetic associations through candidate gene studies3–7, large-scale genome-wide association studies (GWAS) of fasting BCF8,9 or functional islet studies on T2D risk variants10–14. Nonetheless, GWAS focused on BCF traits derived from oral glucose tolerance test (OGTT) data have been limited in sample size15,16 and have often overlooked the potential for related traits to capture distinct genetic features of insulin-producing β-cells17,18. We reasoned that investigating the genetic basis of multiple BCF estimates could provide a broader understanding of β-cell physiology. Here, we aggregate GWAS data of eight OGTT-based BCF traits from ~26,000 individuals of European descent, identifying 55 independent genetic associations at 44 loci. By examining the effects of BCF genetic signals on related phenotypes, we uncover diverse disease mechanisms whereby genetic regulation of BCF may influence T2D risk. Integrating BCF-GWAS data with pancreatic islet transcriptomic and epigenomic datasets reveals 92 candidate effector genes. Gene silencing in β-cell models highlights ACSL1 and FAM46C as key regulators of insulin secretion. Overall, our findings yield insights into the biology of insulin release and the molecular processes linking BCF to T2D risk, shedding light on the heterogeneity of T2D pathophysiology. In a genome-wide association study for traits related to pancreatic beta-cell function in 26,000 individuals, 55 independent associations mapping to 44 genetic loci are identified.","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":18.9,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s42255-024-01140-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142443866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetics brings new insight to β-cell function 遗传学为了解β细胞功能提供了新视角
IF 18.9 1区 医学
Nature metabolism Pub Date : 2024-10-17 DOI: 10.1038/s42255-024-01131-7
Amélie Bonnefond, Philippe Froguel
{"title":"Genetics brings new insight to β-cell function","authors":"Amélie Bonnefond,&nbsp;Philippe Froguel","doi":"10.1038/s42255-024-01131-7","DOIUrl":"10.1038/s42255-024-01131-7","url":null,"abstract":"A meta-analysis of genome-wide association study for eight traits related to pancreatic β-cell function, based on 26,000 individuals, identified 55 independent association signals mapping to 44 loci. This study highlighted new effectors of β-cell function.","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":18.9,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142443864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Itaconate modulates mitochondria for antiviral IFN-β 伊塔康酸调节线粒体,促进 IFN-β 的抗病毒作用
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-15 DOI: 10.1038/s42255-024-01146-0
Thekla Cordes, Karsten Hiller
{"title":"Itaconate modulates mitochondria for antiviral IFN-β","authors":"Thekla Cordes, Karsten Hiller","doi":"10.1038/s42255-024-01146-0","DOIUrl":"https://doi.org/10.1038/s42255-024-01146-0","url":null,"abstract":"Itaconate is an immunomodulatory metabolite that influences the outcome of infections and inflammatory diseases. New evidence indicates that itaconate-induced inhibition of succinate dehydrogenase regulates type 1 interferon production via the release of mitochondrial RNA, linking TCA cycle modulation to antiviral interferon responses.","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytosolic acetyl-CoA synthesis shields mitochondria from stress in brown adipocytes 细胞膜乙酰-CoA 合成保护线粒体免受棕色脂肪细胞压力的影响
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-15 DOI: 10.1038/s42255-024-01152-2
{"title":"Cytosolic acetyl-CoA synthesis shields mitochondria from stress in brown adipocytes","authors":"","doi":"10.1038/s42255-024-01152-2","DOIUrl":"https://doi.org/10.1038/s42255-024-01152-2","url":null,"abstract":"Brown adipose tissue (BAT) facilitates thermogenesis through fatty acid oxidation (FAO). Paradoxically, BAT simultaneously increases anabolic fatty acid synthesis (FAS), the reason for which is unclear. We provide evidence that thermogenic mitochondria within brown adipocytes export TCA cycle intermediates that fuel de novo lipid synthesis, in part to protect against metabolic stress.","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Itaconate drives mtRNA-mediated type I interferon production through inhibition of succinate dehydrogenase 伊塔康酸通过抑制琥珀酸脱氢酶驱动 mtRNA 介导的 I 型干扰素产生
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-15 DOI: 10.1038/s42255-024-01145-1
Shane M. O’Carroll, Christian G. Peace, Juliana E. Toller-Kawahisa, Yukun Min, Alexander Hooftman, Sara Charki, Louise Kehoe, Maureen J. O’Sullivan, Aline Zoller, Anne F. Mcgettrick, Emily A. Day, Maria Simarro, Neali Armstrong, Justin P. Annes, Luke A. J. O’Neill
{"title":"Itaconate drives mtRNA-mediated type I interferon production through inhibition of succinate dehydrogenase","authors":"Shane M. O’Carroll, Christian G. Peace, Juliana E. Toller-Kawahisa, Yukun Min, Alexander Hooftman, Sara Charki, Louise Kehoe, Maureen J. O’Sullivan, Aline Zoller, Anne F. Mcgettrick, Emily A. Day, Maria Simarro, Neali Armstrong, Justin P. Annes, Luke A. J. O’Neill","doi":"10.1038/s42255-024-01145-1","DOIUrl":"https://doi.org/10.1038/s42255-024-01145-1","url":null,"abstract":"<p>Itaconate is one of the most highly upregulated metabolites in inflammatory macrophages and has been shown to have immunomodulatory properties. Here, we show that itaconate promotes type I interferon production through inhibition of succinate dehydrogenase (SDH). Using pharmacological and genetic approaches, we show that SDH inhibition by endogenous or exogenous itaconate leads to double-stranded mitochondrial RNA (mtRNA) release, which is dependent on the mitochondrial pore formed by VDAC1. In addition, the double-stranded RNA sensors MDA5 and RIG-I are required for IFNβ production in response to SDH inhibition by itaconate. Collectively, our data indicate that inhibition of SDH by itaconate links TCA cycle modulation to type I interferon production through mtRNA release.</p>","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Brown fat ATP-citrate lyase links carbohydrate availability to thermogenesis and guards against metabolic stress 棕色脂肪 ATP-柠檬酸裂解酶将碳水化合物的供应与产热联系起来,并抵御代谢压力
IF 20.8 1区 医学
Nature metabolism Pub Date : 2024-10-14 DOI: 10.1038/s42255-024-01143-3
Ekaterina D. Korobkina, Camila Martinez Calejman, John A. Haley, Miranda E. Kelly, Huawei Li, Maria Gaughan, Qingbo Chen, Hannah L. Pepper, Hafsah Ahmad, Alexander Boucher, Shelagh M. Fluharty, Te-Yueh Lin, Anoushka Lotun, Jessica Peura, Sophie Trefely, Courtney R. Green, Paula Vo, Clay F. Semenkovich, Jason R. Pitarresi, Jessica B. Spinelli, Ozkan Aydemir, Christian M. Metallo, Matthew D. Lynes, Cholsoon Jang, Nathaniel W. Snyder, Kathryn E. Wellen, David A. Guertin
{"title":"Brown fat ATP-citrate lyase links carbohydrate availability to thermogenesis and guards against metabolic stress","authors":"Ekaterina D. Korobkina, Camila Martinez Calejman, John A. Haley, Miranda E. Kelly, Huawei Li, Maria Gaughan, Qingbo Chen, Hannah L. Pepper, Hafsah Ahmad, Alexander Boucher, Shelagh M. Fluharty, Te-Yueh Lin, Anoushka Lotun, Jessica Peura, Sophie Trefely, Courtney R. Green, Paula Vo, Clay F. Semenkovich, Jason R. Pitarresi, Jessica B. Spinelli, Ozkan Aydemir, Christian M. Metallo, Matthew D. Lynes, Cholsoon Jang, Nathaniel W. Snyder, Kathryn E. Wellen, David A. Guertin","doi":"10.1038/s42255-024-01143-3","DOIUrl":"https://doi.org/10.1038/s42255-024-01143-3","url":null,"abstract":"<p>Brown adipose tissue (BAT) engages futile fatty acid synthesis–oxidation cycling, the purpose of which has remained elusive. Here, we show that ATP-citrate lyase (ACLY), which generates acetyl-CoA for fatty acid synthesis, promotes thermogenesis by mitigating metabolic stress. Without ACLY, BAT overloads the tricarboxylic acid cycle, activates the integrated stress response (ISR) and suppresses thermogenesis. ACLY’s role in preventing BAT stress becomes critical when mice are weaned onto a carbohydrate-plentiful diet, while removing dietary carbohydrates prevents stress induction in ACLY-deficient BAT. ACLY loss also upregulates fatty acid synthase (<i>Fasn</i>); yet while ISR activation is not caused by impaired fatty acid synthesis per se, deleting <i>Fasn</i> and <i>Acly</i> unlocks an alternative metabolic programme that overcomes tricarboxylic acid cycle overload, prevents ISR activation and rescues thermogenesis. Overall, we uncover a previously unappreciated role for ACLY in mitigating mitochondrial stress that links dietary carbohydrates to uncoupling protein 1-dependent thermogenesis and provides fundamental insight into the fatty acid synthesis–oxidation paradox in BAT.</p>","PeriodicalId":19038,"journal":{"name":"Nature metabolism","volume":null,"pages":null},"PeriodicalIF":20.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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