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Preliminary evaluation of the electrolyte analyzer XI-921 using the EP10-A protocol
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.016
高月亭, 陈涛, 刘汉欣, 刘忠民, 李宁, 肖洪广
{"title":"Preliminary evaluation of the electrolyte analyzer XI-921 using the EP10-A protocol","authors":"高月亭, 陈涛, 刘汉欣, 刘忠民, 李宁, 肖洪广","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.016","url":null,"abstract":"目的 应用EP10-A初步评价XI-921型电解质分析仪的多方面性能.方法 依照《定量临床检验方法的初步评价:批准指南(EP10-A)》,使用欣越华XI-921型电解质分析仪测定血清钾.按要求每天对高、中、低定值样本进行测定,计算偏差、总不精密度,及其截距、斜率、非线性、互染率、漂移性,并进行t检验.结果 偏差:高值0.036 mmol/L、中值0.058 mmol/L、低值0.047 mmol/L.总不精密度:高值0.65%、中值0.37%、低值0.50%.截距:0.083 mmol/L(P＜0.01).线性:0.992(P＜0.01).互染率:-0.036 mmol/L(P＞0.01).非线性:-0.019(P＞0.01).漂移性:0.001(P＞0.01).结论 使用该仪器测定血清钾,偏差、总不精密度均在允许范围内.在一定的范围内有良好妹的线性关系,互染率较低,稳定性很好,各项指标均能满足临床要求。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126840848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Apoptosis of peripheral blood lymphocytes and its significance in patients with rheumatoid arthritis 类风湿关节炎患者外周血淋巴细胞凋亡及其意义
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.004
陶怡, 陈瑞林, 黄成辉
{"title":"Apoptosis of peripheral blood lymphocytes and its significance in patients with rheumatoid arthritis","authors":"陶怡, 陈瑞林, 黄成辉","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.004","url":null,"abstract":"目的 研究类风湿关节炎(RA)患者外周血淋巴细胞的凋亡及其意义.方法 采用流式细胞术和激光共聚焦显微镜,检测35例RA患者和30例健康志愿者的外周血淋巴细胞抵抗凋亡的能力,包括分析新鲜分离和培养的淋巴细胞凋亡率及其与临床病情严重度的相关性.结果 两组间新鲜分离的淋巴细胞凋亡率差异无统计学意义[(2.63±1.47)%比(3.53±2.04)%,P＞0.05].活化淋巴细胞检测的结果显示,RA组的凋亡率明显低于对照组[(12.08±6.06)%比(18.66±7.27)%,P＜0.05],并与DAS28评分呈负相关(r=-0.617,P=0.008).结论 RA患者活化的外周血淋巴细胞出现凋亡缺陷,并与RA病情活动度相关.提示这些细胞募集到组织损害和自身免疫炎性反应进程异常的部位并对炎性反应进程的发展和维持起重要作用。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121891536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of biological features on leuckemia stem cell 白血病干细胞生物学特性的研究
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.014
叶静梅, 王春燕, 于宝丹, 谭获
{"title":"Study of biological features on leuckemia stem cell","authors":"叶静梅, 王春燕, 于宝丹, 谭获","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.014","url":null,"abstract":"目的 通过对白血病原代细胞的体外培养,研究白血病干细胞(LSC)的生物学特性.方法 分离18例白血病患者骨髓或外周血中单个核细胞(MNC),在体外进行培养观察,并用流式细胞术方法检测分离的MNC中LSC的数量及和细胞表面标记.结果 从白血病患者中分离的MNC在体外培养1周后基本死亡.流式检测分离的MNC中免疫表型是CD34+CD38-CD123+的细胞群约有0.676%(0.03%～2%).结论 白血病干细胞难以在体外培养生长及扩增.外周血及骨髓中分离白血病患者的单个核细胞中,极少部分细胞表达干细胞标志。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127044160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of different concertrations of ozone injection on pathological changes of rabbit skeletal muscle 不同浓度臭氧注射对家兔骨骼肌病理改变的影响
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.009
李韶辉, 余革, 陈国勤
{"title":"Impact of different concertrations of ozone injection on pathological changes of rabbit skeletal muscle","authors":"李韶辉, 余革, 陈国勤","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.009","url":null,"abstract":"目的 观察两种浓度的臭氧(03)对实验兔骨骼肌组织的病理变化的影响,以评估臭氧对肌纤维的氧化程度.方法 10只实验兔随机分为A、B两组.随机选择一侧大腿肌肉注射臭氧-氧气混合气体,A组为浓度50μg/ml,B组为浓度30μg/ml两组分别注射5 ml气体.注射臭氧后0.5、2 h和2周后分别对臭氧注射点、旁开1 cm和2 cm取活检组织行病理学光镜检查,对侧大腿相对应部位取活检作空白对照.结果 与空白对照组比较,病理光镜下显示A、B两组注射臭氧后0.5 h注射点、旁开1 cm见肌细胞肿胀,间隙变窄或不清,肌细胞边界不清,横纹紊乱,部分肌细胞坏死溶解,空泡变性;2 h后注射点、旁开1 cm处在肌细胞坏死溶解的基础上出现了肌细胞间隙和血管内炎症细胞浸润;2周后注射点、旁开1 cm切片显示肌细胞明显萎缩,大量纤维组织增生,部分伴有玻璃样变.上述3个时点的注射点旁开2 cm病理切片均未见异常.结论 两种浓度的臭氧肌肉注射后会产生程度相当的肌细胞损害,范围半径约2 cm,可能对炎性肌细胞或肌纤维化组织起治疗作用,2周后纤维组织损伤修复。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113953701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical pharmacodynamics of rocuronium: orbicularis oculi versus adductor pollicis 罗库溴铵的临床药效学:眼轮匝肌与拇内收肌
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.008
冉建, 欧阳葆怡
{"title":"Clinical pharmacodynamics of rocuronium: orbicularis oculi versus adductor pollicis","authors":"冉建, 欧阳葆怡","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.008","url":null,"abstract":"目的 比较罗库溴铵对眼轮匝肌和拇收肌临床药效学的差异.方法 选择心、肺、肝、肾和神经肌肉功能基本正常的30～50岁全麻普外及妇科手术患者男女各10例,丙泊酚和芬太尼维持麻醉.静注罗库溴铵0.3 mg/kg后,记录同侧眼轮匝肌和拇收肌诱发颤搐反应的抑制过程和恢复过程.结果 拇收肌的显效时间和达到最大抑制程度的时间[(41.0±8.3)s和(241.3±60.8)s]与眼轮匝肌[(42.0±7.9)s和(276.0±62.6)s]基本相同(P＞0.05),而最大抑制程度(95.3%±5.2%)比服轮匝肌(67.7%±15.3%)显著增高(P＜0.01).T1(4个成串刺激中的第1个肌颤搐反应)恢复到基础值50%和75%的时间,拇收肌[(19.8±5.1)min和(24.1±6.2)min]与眼轮匝肌的[(17.5±8.7)min和(27.3±8.6)min]差异无统计学意义(P＞0.05),但T1恢复到基础值95%和100%的时间,拇收肌[(29.2±7.6)min和(31.1±8.3)min]明显比眼轮匝肌[(37.4±9.0)min和(40.0±9.1)min]快,差异有统计学意义(P＜0.01).TOFr恢复到基础值80%的时间,拇收肌[(30.2±8.1)min]和眼轮匝肌[(28.8±8.8)min]基本相同(P＞0.05).结论 中青年患者给予1倍ED95罗库溴铵对眼轮匝肌和拇收肌阻滞过程的差异主要表现在眼轮匝肌最大阻滞程度显著比拇收肌低,以及肌肉松弛效应完全消除时间显著比拇收肌长,而起效时间则基本相同。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115291504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of microsurgery treatment for hypertensive cerebral hemorrhage with intraventricular hemorrhage 显微外科治疗高血压脑出血合并脑室内出血的疗效评价
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.015
向进, 郭伟, 王莉莉, 杨地, 吕文
{"title":"Evaluation of microsurgery treatment for hypertensive cerebral hemorrhage with intraventricular hemorrhage","authors":"向进, 郭伟, 王莉莉, 杨地, 吕文","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.015","url":null,"abstract":"目的 探讨高血压脑出血破入脑室的手术治疗方法.方法 采取大骨瓣开颅显微镜下血肿清除术和小骨窗开颅显微镜下血肿清除术对38例高血压脑出血破入脑室的患者行手术治疗.结果 术后存活33例,死亡5例(13%).随访存活患者,按ADL评分:Ⅱ级10例(26%),Ⅲ级15例(39%),Ⅳ级5例(13%),V级3例(8%).结论 对早期高血压脑出血量＞30 ml破人脑室并发脑疝者应尽早行大骨瓣开颅显微镜下血肿清除术,对于出血量＜30 ml或形状不规则破入脑室应在严密观察下行保守治疗＞24 h后取小骨窗微创手术清除血肿,可降低死残率,提高生存质量。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127156500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ankylosing spondylitis gene mapping defined by genome search meta analysis 基因组搜索meta分析定义强直性脊柱炎基因定位
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.002
黄进贤, 古洁若
{"title":"Ankylosing spondylitis gene mapping defined by genome search meta analysis","authors":"黄进贤, 古洁若","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.002","url":null,"abstract":"目的 综合分析强直性脊柱炎(ankylosing spondylitis,AS)家系的全基因组扫描基因定位的结果,探讨进一步研究和确定AS的遗传易感位点的思路和方法.方法 采用基因组寻证荟萃分析(genome search meta analysis,GSMA)法,对符合该方法分析的4个AS家系的全基因组扫描结果包括参数连锁分析和非参数连锁分析的结果进行分析.根据连锁分析的最大值被赋值并按降序排列,从而计算出总的等级分值(Rsumrnk).根据每个研究的受累个体数进行加权GSMA对结果进行修正.本研究共有479个家系,1151例AS患者.总的等级分析概率(Psumrnk)和有序的等级分析概率(Pord)＜0.05被认为可能含有易感区域;GSMA Psumrnk＜0.000417时,被认为在全基因组连锁中有显著意义.结果 GSMA显示10组结果的Psumrnk和Pord均小于0.05,表明这些组内最可能含有AS的易感基因位点,这些组分别是6.2、16.3、6.1、3.3、6.3、16.4、10.5、17.1、2.5、2.9.GSMA生成的具有显著意义的全基因组连锁分析的证据位于6p22.3-p21.1(BIN6.2,Psumrnk＜0.000417),包含HLA位点.结论 本GSMA研究进一步证实了HLA位点是AS的主要易感区域,并初步提供非HLA区域包括16 q,3 p,10 q,2 p,17 p和2 q证据。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129651979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Comparative study of the transient hepatic abnormal enhancement during dynamic contrast-enhanced spiral CT between tumor and inflammatory disease 肿瘤与炎性疾病螺旋CT动态增强时肝脏一过性异常强化的对比研究
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.013
管祥林, 刘林, 梁英萍, 冉慕光, 周世柱
{"title":"Comparative study of the transient hepatic abnormal enhancement during dynamic contrast-enhanced spiral CT between tumor and inflammatory disease","authors":"管祥林, 刘林, 梁英萍, 冉慕光, 周世柱","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.013","url":null,"abstract":"目的 探讨不同肝脏病变间动态增强CT扫描肝实质一过性异常强化(THAE)的表现特征及临床意义.方法 收集肝脏或腹部病变为肿瘤类和炎性病变并伴有动态CT扫描肝实质一过性异常强化征象病例43例,所有病例病变均由病理或临床资料证实.通过分组并对肝实质一过性异常强化征的形态、分布部位、强化特点等多方面进行对比分析.结果 肿瘤组THAE征均呈楔形、不规则形或节段形,分布于病灶一侧或远离病灶,边界多清楚.炎症组引起的THPE多呈大叶、大片或斑片状,与炎性病灶相临或环绕炎性病灶分布,边界欠清.肿瘤组与炎性病变组对比,动态增强扫描时动脉期肝实质一过性异常强化征在形态和发生部位方面差异性具有统计学意义.结论 不同病变伴有特征的动脉期肝实质一过性异常强化征的表现,对于肝内病变的定性和鉴别诊断有参考价值。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"161 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133799285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
经皮经肝胆道镜治疗良性肝内胆管狭窄的现状及进展 经皮经肝胆道镜治疗良性肝内胆管狭窄的现状及进展
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.019
侯湘德, 刘衍民
{"title":"经皮经肝胆道镜治疗良性肝内胆管狭窄的现状及进展","authors":"侯湘德, 刘衍民","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.06.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.06.019","url":null,"abstract":"良性肝内胆管狭窄是目前胆道外科处理的难点之一,以往主要采用肝叶切除及肝内胆管狭窄切开整形、胆肠吻合术治疗,但手术创伤较大,尤其对那些有手术史的患者,反复的开腹手术困难越来越大,患者也难以接受。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122835632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HLA-B27与脊柱关节炎发病机制研究进展 HLA-B27与脊柱关节炎发病机制研究进展
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-12-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.06.018
吴震, 古洁若
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