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Journal of Modern Clinical Medical Bioengineering最新文献

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Establishment and evaluation of the method for removing the high-abundance proteins in cerebrospinal fluid proteomics 脑脊液蛋白质组学中高丰度蛋白去除方法的建立与评价
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.009
李焕敏, 邵明, 谭红愉, 吴卓华, 孔天翰
{"title":"Establishment and evaluation of the method for removing the high-abundance proteins in cerebrospinal fluid proteomics","authors":"李焕敏, 邵明, 谭红愉, 吴卓华, 孔天翰","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.009","url":null,"abstract":"目的 建立和评价脑脊液蛋白质组学中高丰度蛋白的去除方法.方法 使用Oasis HLB(hydrophilic-lipophilic balance)过滤柱,运用反相固相法,根据蛋白质不同的疏水性分馏脑脊液,去除高丰度蛋白和盐.将含有低丰度蛋白的过滤液冻干,利用双向凝胶电泳(2-DE)分析评价脑脊液蛋白层析去除高丰度蛋白的效果.结果 通过Oasis HLB柱处理可将大部分高丰度蛋白去除.2-DE图谱中低丰度蛋白质分辨率明显增加,点数增多59.86%.结论 本研究建立的脑脊液高丰度蛋白去除方法可有效地应用于疾病的脑脊液蛋白质组学研究。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115390877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of backup and archiving methods of DSA image DSA图像备份与归档方法的探讨
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.012
林海波, 宋玉全, 李新春
{"title":"Investigation of backup and archiving methods of DSA image","authors":"林海波, 宋玉全, 李新春","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.012","url":null,"abstract":"目的 探讨适合数字减影血管造影(DSA)图像备份存储的最简便和最实惠的存储方式.方法 将429例具有完整临床资料患者的DSA图像资料进行光盘刻录、图像存储和传输系统(PACS)存储和USB移动存储.对3种方法的备份耗费的时间、调阅所需时间、所耗费用、操作的简便性及图像质量进行比较.结果 USB移动储蓄的备份所需时间、调阅所需时间、费用、每例患者备份成本明显低于前两种方法,而且操作简单、图像质量好.结论 USB移动存储是最简便和最实惠的DSA图像数据存储方式,可作为常规方法加以推广。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116040900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Change of P(a-ET)CO2 in pafients undergoing cardiopulmonary bypass 体外循环患者P(a-ET)CO2的变化
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.006
肖辉, 佘守章, 许立新
{"title":"Change of P(a-ET)CO2 in pafients undergoing cardiopulmonary bypass","authors":"肖辉, 佘守章, 许立新","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.006","url":null,"abstract":"目的 探讨不同类型心脏患者心内直视手术期间呼气末二氧化碳分压(PETCO2)与动脉血二氧化碳分压(PaCO2)的相关性及二者差值[P(a-ET)CO2]的临床意义.方法 45例患者分成3组,每组15例.A组:左向右分流组;B组:无分流组;C组:右向左分流组.3组分别于术前(T0)、插管后15 min(T1)、手术开始时(T2)、转流前(T3)、转流停止后15 min(T4)、术毕时(T5)共6个时点,用旁气流法监测PETCO2,同时抽动脉血测定PaCO2,应用直线回归分析3组各时点PET,CO2与PaCO2的相关性,同时计算并分析各组不同时点的P(a-ET)CO2.结果 (1)A组、B组各时点PETCO2与PaCO2呈正相关,A组r为0.70～0.85(P＜0.01),B组r为0.71～0.79(P＜0.01);C组麻醉开始至转停后PETCO2与PaCO2均无相关(0.46～0.54,P＞0.05),而到术毕时则呈正相关(r=0.66,P＜0.05).(2)3组患者转流前的P(a-ET) CO2变化不大,停转流后A组、B组P(a-ET)CO2增加,其中以A组增加较明显(P＜0.01),而C组P(a-ET)CO2显著减少(P＜0.05).结论 无分流或左向右分流心脏患者在体外循环转流前后PETCO2与PaCO2的相关性较好;右向左分流心脏患者转流前的相关性较差,心内畸形纠正后其相关性可发生变化.P(a-ET)CO2在不同的心脏患者之间差异较大,必须与PaCO2相结合,才能正确指导临床。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127759600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
模拟失重或失重对肺循环和呼吸功能的影响 模拟失重或失重对肺循环和呼吸功能的影响
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.015
汪德生, 王惠娟
{"title":"模拟失重或失重对肺循环和呼吸功能的影响","authors":"汪德生, 王惠娟","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.015","url":null,"abstract":"","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124672108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
尾加压素II与肾脏疾病的研究进展 尾加压素II与肾脏疾病的研究进展
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.018
刘长波, 汪华林
{"title":"尾加压素II与肾脏疾病的研究进展","authors":"刘长波, 汪华林","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.018","url":null,"abstract":"","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116244653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
胃肠道问质瘤的诊断及治疗 胃肠道问质瘤的诊断及治疗
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.017
巴明臣, 崔书中
{"title":"胃肠道问质瘤的诊断及治疗","authors":"巴明臣, 崔书中","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.017","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.017","url":null,"abstract":"胃肠道间质瘤(GIST)是胃肠道最常见的间叶组织性肿瘤,具有非定向分化的特性,由于组织分化程度的不同,有着不同的组织学、形态学和预后特征.20世纪90年代以前所报道的所谓平滑肌瘤、神经内分泌肿瘤经再次免疫组化证实85%以上为间质瘤[1].为了使临床医师对GIST有一个总体认识,本文仅对GIST的发生机制诊断及治疗现状作一简要概述。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131233255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical application of perfusion imaging in peritumoral edema of cerebral neoplasms with multislice spiral CT 多层螺旋CT灌注显像在脑肿瘤瘤周水肿中的临床应用
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.007
蒋山姗, 李扬彬, 谭理连, 陈德基
{"title":"Clinical application of perfusion imaging in peritumoral edema of cerebral neoplasms with multislice spiral CT","authors":"蒋山姗, 李扬彬, 谭理连, 陈德基","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.007","url":null,"abstract":"目的 探讨瘤周水肿的多层螺旋CT灌注成像(CTPI)在脑肿瘤诊断方面的应用价值.方法 采用Sensation 16层螺旋CT机对本院29例脑肿瘤患者(胶质瘤14例,转移瘤7例,脑膜瘤8例)行CTPI,分别测量不同瘤周水肿区以及对侧正常脑组织的脑血流量(CBF)、脑血容量(CBV)、肿瘤表面渗透性(PS)、对比剂峰值时间(time to peak,TTP),并进行比较.结果 转移瘤和脑膜瘤瘤周水肿的CBV、CBF显著低于对侧正常脑组织(P＜0.05),PS差异则无统计学意义(P＞0.05).与对侧正常脑组织比较,胶质瘤瘤周水肿的CBV差异无统计学意义(P＞0.05),而PS明显升高(P＜0.05),CBF降低(P＜0.05).胶质瘤、转移瘤及脑膜瘤3者瘤周水肿的TTP甲值均较对侧正常脑组织显著延长(P＜0.05).结论 脑肿瘤的CTPI检查能定量反映瘤周水肿区的血流动力学状况,对脑肿瘤的鉴别和预测预后有重要临床意义。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124350004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction of specific siRNA expression vector of estrogen receptor α subunit gene 雌激素受体α亚基基因特异性siRNA表达载体的构建
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.004
张昊, 武兆忠, 章乐虹
{"title":"Construction of specific siRNA expression vector of estrogen receptor α subunit gene","authors":"张昊, 武兆忠, 章乐虹","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.004","url":null,"abstract":"目的 探讨构建雌激素受体α(ERα)亚基基因小干扰RNA(siRNA)表达载体的方法,为建立基因敲除ERα亚基突变株提供素材和依据.方法 利用计算机辅助设计ERα特异性siRNA,体外合成siRNA基因,并将其定向克隆入pSilencer 4.1-CMV质粒中,构建真核表达载体.采用PCR扩增、酶切分析鉴定.结果 双酶切法鉴定重组质粒后,RT-PCR法鉴定ERαsiRNA载体可特异地抑制人成骨样细胞株MG63细胞中ERα的表达.结论 ERα亚基特异性siRNA表达载体被成功构建,为进一步研究雌激素受体在骨代谢中的作用机制提供了实验素材和依据。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134261043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Laparoscopy-assisted left lateral liver resection for hepatolithiasis 腹腔镜辅助左肝切除治疗肝内胆管结石
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.010
孙北望, 刘衍民, 王平, 曾可伟
{"title":"Laparoscopy-assisted left lateral liver resection for hepatolithiasis","authors":"孙北望, 刘衍民, 王平, 曾可伟","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.010","url":null,"abstract":"目的 总结腹腔镜辅助下左肝外叶切除治疗胆管结石的临床经验.方法 自2002年6月至2005年10月期间,本院左肝内胆管结石患者21例.患者在腹腔镜辅助下行左肝外叶部分切除及胆道镜探查取石术,部分加做胆囊切除术.结果 全部21例行腹腔镜辅助下左肝外叶切除术,17例联合行腹腔镜胆囊切除术.手术时间为90～190 min,平均(140±36)min.手术中出血量为100～250 ml,平均(160±52)m1.术后恢复顺利,术后平均住院(6.2±1.2)d.结论 腹腔镜辅助下行左肝外叶部分切除治疗左肝胆管结石是安全可行的.该术式容易掌握,风险小,为治疗左肝胆管结石提供了一个新方法。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115999171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-tumor effect of adeno-associated viral vector-mediated systemic delivery of MDA-7 on human hepatocellular carcinoma cells 腺相关病毒载体介导的MDA-7全身递送对人肝癌细胞的抗肿瘤作用
Journal of Modern Clinical Medical Bioengineering Pub Date : 2007-04-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2007.02.003
朱光辉, 徐波, 夏金堂, 翁杰锋, 李书华
{"title":"Anti-tumor effect of adeno-associated viral vector-mediated systemic delivery of MDA-7 on human hepatocellular carcinoma cells","authors":"朱光辉, 徐波, 夏金堂, 翁杰锋, 李书华","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.003","url":null,"abstract":"目的 观察PEG启动子调控腺相关病毒介导的黑色素瘤分化相关基因7(MDA-7)在体内外抑制肝癌细胞的生物学活性,探讨重组腺相关病毒介导MDA-7基因用于肝癌基因治疗的应用前景.方法 构建重组腺相关病毒rAAV-PEG-MDA-7表达系统,体外感染人肝癌细胞株HepG2细胞.Western印迹检测转染细胞内MDA-7蛋白;MTT法检测细胞增殖抑制率;流式细胞术分析细胞周期和细胞凋亡变化.构建肝癌细胞HepG2裸鼠皮下移植瘤模型,尾静脉注射rAAV-PEG-MDA-7,观察其对肝癌生长的抑制作用;ELISA方法检测血浆MDA-7蛋白浓度;TUNEL法分析MDA-7对肿瘤细胞的凋亡诱导情况;免疫组织化学分析MDA-7在肿瘤组织中的表达.结果 重组腺相关病毒rAAV-PEG-MDA-7可特异性转染HepG2细胞,MDA-7蛋白在HepG2细胞中高效表达,并呈时间依赖性.重组腺相关病毒rAAV-PEG-MDA-7可抑制HepG2细胞增殖并诱导其凋亡,GO/G1期细胞百分比明显增多,G2/M期的细胞显著减少(P＜0.05).全身系统性给予rAAV-PEG-MDA-7后,血清中可持续检测到MDA-7蛋白,且注射后2周浓度达高峰(200 ng/m1);肿瘤生长受抑制,抑瘤率为62%(P＜0.05);免疫组化结果显示MDA-7在肿瘤组织中表达;TUNEL结果显示rAAV-PEG-MDA-7可诱导肿瘤细胞凋亡.结论 构建出的重组腺相关病毒rAAV-PEG-MDA-7表达系统具有良好的肿瘤靶向性,通过抑制肝癌细胞增殖和诱导其凋亡发挥抗肝癌作用。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121903328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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