{"title":"脑脊液蛋白质组学中高丰度蛋白去除方法的建立与评价","authors":"李焕敏, 邵明, 谭红愉, 吴卓华, 孔天翰","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.009","DOIUrl":null,"url":null,"abstract":"目的 建立和评价脑脊液蛋白质组学中高丰度蛋白的去除方法.方法 使用Oasis HLB(hydrophilic-lipophilic balance)过滤柱,运用反相固相法,根据蛋白质不同的疏水性分馏脑脊液,去除高丰度蛋白和盐.将含有低丰度蛋白的过滤液冻干,利用双向凝胶电泳(2-DE)分析评价脑脊液蛋白层析去除高丰度蛋白的效果.结果 通过Oasis HLB柱处理可将大部分高丰度蛋白去除.2-DE图谱中低丰度蛋白质分辨率明显增加,点数增多59.86%.结论 本研究建立的脑脊液高丰度蛋白去除方法可有效地应用于疾病的脑脊液蛋白质组学研究。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"17 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Establishment and evaluation of the method for removing the high-abundance proteins in cerebrospinal fluid proteomics\",\"authors\":\"李焕敏, 邵明, 谭红愉, 吴卓华, 孔天翰\",\"doi\":\"10.3760/CMA.J.ISSN.1008-634X.2007.02.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"目的 建立和评价脑脊液蛋白质组学中高丰度蛋白的去除方法.方法 使用Oasis HLB(hydrophilic-lipophilic balance)过滤柱,运用反相固相法,根据蛋白质不同的疏水性分馏脑脊液,去除高丰度蛋白和盐.将含有低丰度蛋白的过滤液冻干,利用双向凝胶电泳(2-DE)分析评价脑脊液蛋白层析去除高丰度蛋白的效果.结果 通过Oasis HLB柱处理可将大部分高丰度蛋白去除.2-DE图谱中低丰度蛋白质分辨率明显增加,点数增多59.86%.结论 本研究建立的脑脊液高丰度蛋白去除方法可有效地应用于疾病的脑脊液蛋白质组学研究。\",\"PeriodicalId\":189947,\"journal\":{\"name\":\"Journal of Modern Clinical Medical Bioengineering\",\"volume\":\"17 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Modern Clinical Medical Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Modern Clinical Medical Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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