{"title":"雌激素受体α亚基基因特异性siRNA表达载体的构建","authors":"张昊, 武兆忠, 章乐虹","doi":"10.3760/CMA.J.ISSN.1008-634X.2007.02.004","DOIUrl":null,"url":null,"abstract":"目的 探讨构建雌激素受体α(ERα)亚基基因小干扰RNA(siRNA)表达载体的方法,为建立基因敲除ERα亚基突变株提供素材和依据.方法 利用计算机辅助设计ERα特异性siRNA,体外合成siRNA基因,并将其定向克隆入pSilencer 4.1-CMV质粒中,构建真核表达载体.采用PCR扩增、酶切分析鉴定.结果 双酶切法鉴定重组质粒后,RT-PCR法鉴定ERαsiRNA载体可特异地抑制人成骨样细胞株MG63细胞中ERα的表达.结论 ERα亚基特异性siRNA表达载体被成功构建,为进一步研究雌激素受体在骨代谢中的作用机制提供了实验素材和依据。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"30 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2007-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Construction of specific siRNA expression vector of estrogen receptor α subunit gene\",\"authors\":\"张昊, 武兆忠, 章乐虹\",\"doi\":\"10.3760/CMA.J.ISSN.1008-634X.2007.02.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"目的 探讨构建雌激素受体α(ERα)亚基基因小干扰RNA(siRNA)表达载体的方法,为建立基因敲除ERα亚基突变株提供素材和依据.方法 利用计算机辅助设计ERα特异性siRNA,体外合成siRNA基因,并将其定向克隆入pSilencer 4.1-CMV质粒中,构建真核表达载体.采用PCR扩增、酶切分析鉴定.结果 双酶切法鉴定重组质粒后,RT-PCR法鉴定ERαsiRNA载体可特异地抑制人成骨样细胞株MG63细胞中ERα的表达.结论 ERα亚基特异性siRNA表达载体被成功构建,为进一步研究雌激素受体在骨代谢中的作用机制提供了实验素材和依据。\",\"PeriodicalId\":189947,\"journal\":{\"name\":\"Journal of Modern Clinical Medical Bioengineering\",\"volume\":\"30 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Modern Clinical Medical Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.004\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Modern Clinical Medical Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2007.02.004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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