Nature Microbiology最新文献

Engineered Metarhizium fungi produce longifolene to attract and kill mosquitoes. 经过改造的绿僵菌真菌产生长叶烯来吸引和杀死蚊子。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-24 DOI: 10.1038/s41564-025-02155-9

Dan Tang,Jiani Chen,Yubo Zhang,Xingyuan Tang,Xinmiao Wang,Chaonan Yu,Xianxian Cheng,Junwei Zhang,Wenqi Shi,Qing Zhen,Shuxing Liu,Yizhou Huang,Jiali Ning,Guoding Zhu,Meichun Zhang,Juping Hu,Etienne Bilgo,Abdoulaye Diabate,Sheng-Hua Ying,Jun Cao,Raymond J St Leger,Jianhua Huang,Weiguo Fang

{"title":"Engineered Metarhizium fungi produce longifolene to attract and kill mosquitoes.","authors":"Dan Tang,Jiani Chen,Yubo Zhang,Xingyuan Tang,Xinmiao Wang,Chaonan Yu,Xianxian Cheng,Junwei Zhang,Wenqi Shi,Qing Zhen,Shuxing Liu,Yizhou Huang,Jiali Ning,Guoding Zhu,Meichun Zhang,Juping Hu,Etienne Bilgo,Abdoulaye Diabate,Sheng-Hua Ying,Jun Cao,Raymond J St Leger,Jianhua Huang,Weiguo Fang","doi":"10.1038/s41564-025-02155-9","DOIUrl":"https://doi.org/10.1038/s41564-025-02155-9","url":null,"abstract":"Chemical insecticides have been the primary method of mosquito control, but in recent years, mosquitoes have become resistant to these compounds. Metarhizium fungi are emerging as promising alternatives and can kill mosquitoes with a small number of spores. It was previously shown that caterpillars affected by fungal infections can attract mosquitoes. However, the mechanisms and potential applications of this attraction are lacking. Here we show that Metarhizium-colonized insect cadavers release the volatile longifolene to attract and infect healthy insects, facilitating spore dispersal. We identified the responsible odorant receptors in Drosophila melanogaster and Aedes albopictus. The virulent mosquito pathogen Metarhizium pingshaense was engineered to express pine longifolene synthase to produce a large amount of longifolene on media. The transgenic spores effectively attracted and killed male and female A. albopictus, Anopheles sinensis and Culex pipiens. Attraction of wild-caught mosquitoes was not impacted by human presence, but mosquito-attracting flowering plants competed with transgenic M. pingshaense for attractiveness, although mortality remained over 90%. This study uncovered an active spore dispersal mechanism in broad-host-range entomopathogenic Metarhizium, enhancing mosquito control efficacy.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"106 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145357954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial TIR-based immune systems sense phage capsids to initiate defense. 细菌基于tir的免疫系统感知噬菌体衣壳启动防御。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-24 DOI: 10.1038/s41564-025-02150-0

Cameron G Roberts,Chloe B Fishman,Zhiying Zhang,Dalton V Banh,Dinshaw J Patel,Luciano A Marraffini

{"title":"Bacterial TIR-based immune systems sense phage capsids to initiate defense.","authors":"Cameron G Roberts,Chloe B Fishman,Zhiying Zhang,Dalton V Banh,Dinshaw J Patel,Luciano A Marraffini","doi":"10.1038/s41564-025-02150-0","DOIUrl":"https://doi.org/10.1038/s41564-025-02150-0","url":null,"abstract":"Thoeris systems use proteins with Toll/interleukin-1 receptor domains to protect prokaryotes from phage infection through the synthesis of a cyclic adenosine diphosphate ribose signalling molecule, which activates an effector that depletes the host of the essential metabolite NAD+ to limit viral propagation. How infection is recognized during Thoeris immunity is not known. Here we investigate the staphylococcal Thoeris defense system, ThsA-B1-B2, and found that, upon infection, the major capsid proteins of Siphoviridae phages from serogroup B, but not A, form a complex with ThsB1 and ThsB2 to activate Thoeris defense. Thoeris cyclases from Streptococcus also recognize major capsid proteins. Our results suggest that the accumulation of capsid mutations that enable avoidance of Thoeris recognition may be an important evolutionary force behind the structural diversity of prokaryotic viruses. More broadly, given that some mammalian immune pathways contain Toll/interleukin-1 receptor domains that recognize viral structures, our findings highlight a conserved mechanism of innate antiviral immunity.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"42 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145357621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A widespread hydrogenase supports fermentative growth of gut bacteria in healthy people. 广泛存在的氢化酶支持健康人肠道细菌的发酵生长。

IF 19.4 1区生物学

Nature Microbiology Pub Date : 2025-10-23 DOI: 10.1038/s41564-025-02154-w

Caitlin Welsh, Princess R Cabotaje, Vanessa R Marcelino, Thomas D Watts, Duncan J Kountz, Marion Jespersen, Jodee A Gould, Nhu Quynh Doan, James P Lingford, Thilini Koralegedara, Jessica Solari, Gemma L D'Adamo, Ping Huang, Natasha Bong, Emily L Gulliver, Remy B Young, Henrik Land, Kaija Walter, Isaac Cann, Gabriel V Pereira, Eric C Martens, Patricia G Wolf, Jason M Ridlon, H Rex Gaskins, Edward M Giles, Dena Lyras, Rachael Lappan, Gustav Berggren, Samuel C Forster, Chris Greening

{"title":"A widespread hydrogenase supports fermentative growth of gut bacteria in healthy people.","authors":"Caitlin Welsh, Princess R Cabotaje, Vanessa R Marcelino, Thomas D Watts, Duncan J Kountz, Marion Jespersen, Jodee A Gould, Nhu Quynh Doan, James P Lingford, Thilini Koralegedara, Jessica Solari, Gemma L D'Adamo, Ping Huang, Natasha Bong, Emily L Gulliver, Remy B Young, Henrik Land, Kaija Walter, Isaac Cann, Gabriel V Pereira, Eric C Martens, Patricia G Wolf, Jason M Ridlon, H Rex Gaskins, Edward M Giles, Dena Lyras, Rachael Lappan, Gustav Berggren, Samuel C Forster, Chris Greening","doi":"10.1038/s41564-025-02154-w","DOIUrl":"https://doi.org/10.1038/s41564-025-02154-w","url":null,"abstract":"Disruption of hydrogen (H2) cycling in the gut is linked to gastrointestinal disorders, infections and cancers. However, the mechanisms and microorganisms controlling H2 production in the gut remain unresolved. Here we show that gut H2 production is primarily driven by the microbial group B [FeFe]-hydrogenase. Metagenomics and metatranscriptomics of stool and tissue biopsy samples show that hydrogenase-encoding genes are widely present and transcribed in gut bacteria. Assessment of 19 taxonomically diverse gut isolates revealed that the group B [FeFe]-hydrogenases produce large amounts of H2 gas and support fermentative growth of Bacteroidetes and Firmicutes. Further biochemical and spectroscopic characterization of purified enzymes show that they are catalytically active, bind a di-iron active site and reoxidize ferredoxin derived from the pyruvate:ferredoxin oxidoreductase reaction. Group B hydrogenase-encoding genes are significantly depleted in favour of other fermentative hydrogenases in patients with Crohn's disease. Finally, metabolically flexible respiratory bacteria may be the dominant hydrogenotrophs in the gut, rather than acetogens, methanogens and sulfate reducers. These results uncover the enzymes and microorganisms controlling H2 cycling in the healthy human gut.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":" ","pages":""},"PeriodicalIF":19.4,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145355526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying rare and active species that drive carbon turnover in complex microbiotas. 识别稀有和活跃的物种，驱动复杂微生物群的碳周转。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-22 DOI: 10.1038/s41564-025-02170-w

引用次数: 0

Long-read metagenomics for strain tracking after faecal microbiota transplant. 用于粪便微生物群移植后菌株追踪的长读宏基因组学。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-22 DOI: 10.1038/s41564-025-02164-8

Yu Fan,Mi Ni,Varun Aggarwala,Edward A Mead,Magdalena Ksiezarek,Lei Cao,Michael A Kamm,Thomas J Borody,Sudarshan Paramsothy,Nadeem O Kaakoush,Ari Grinspan,Jeremiah J Faith,Gang Fang

{"title":"Long-read metagenomics for strain tracking after faecal microbiota transplant.","authors":"Yu Fan,Mi Ni,Varun Aggarwala,Edward A Mead,Magdalena Ksiezarek,Lei Cao,Michael A Kamm,Thomas J Borody,Sudarshan Paramsothy,Nadeem O Kaakoush,Ari Grinspan,Jeremiah J Faith,Gang Fang","doi":"10.1038/s41564-025-02164-8","DOIUrl":"https://doi.org/10.1038/s41564-025-02164-8","url":null,"abstract":"Accurate tracking of bacterial strains that stably engraft in faecal microbiota transplant (FMT) recipients is critical for understanding the determinants of strain engraftment, evaluating correlations with clinical outcomes and guiding the development of therapeutic consortia. While short-read sequencing has advanced FMT research, it faces challenges in strain-level de novo metagenomic assembly. Here we describe LongTrack, a method that uses long-read metagenomic assemblies for FMT strain tracking. LongTrack shows higher precision and specificity than short-read approaches, especially when multiple strains co-exist in the same sample. We uncovered 648 engrafted strains across six FMT cases involving patients with recurrent Clostridioides difficile infection and inflammatory bowel disease. Furthermore, long reads enabled assessment of the genomic and epigenomic stability of engrafted strains at the 5-year follow-up timepoint, revealing structural variations that may be associated with strain adaptation in a new host environment. Our findings support the use of long-read metagenomics to track microbial strains and their adaptations.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"101 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145339433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity-targeted metaproteomics uncovers rare syntrophic bacteria central to anaerobic community metabolism. 以活动为目标的宏蛋白质组学揭示了罕见的厌氧群落代谢中心的合成细菌。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-21 DOI: 10.1038/s41564-025-02146-w

Skyler Friedline,Elizabeth A McDaniel,Matthew Scarborough,Maxwell Madill,Kate Waring,Vivian S Lin,Rex R Malmstrom,Danielle Goudeau,William Chrisler,Morten K D Dueholm,Leo J Gorham,Chathuri J Kombala,Lydia H Griggs,Heather M Olson,Sophie B Lehmann,Nathalie Munoz,Jesse Trejo,Nikola Tolic,Ljiljana Pasa-Tolic,Sarah M Williams,Mary Lipton,Steven J Hallam,Ryan M Ziels

{"title":"Activity-targeted metaproteomics uncovers rare syntrophic bacteria central to anaerobic community metabolism.","authors":"Skyler Friedline,Elizabeth A McDaniel,Matthew Scarborough,Maxwell Madill,Kate Waring,Vivian S Lin,Rex R Malmstrom,Danielle Goudeau,William Chrisler,Morten K D Dueholm,Leo J Gorham,Chathuri J Kombala,Lydia H Griggs,Heather M Olson,Sophie B Lehmann,Nathalie Munoz,Jesse Trejo,Nikola Tolic,Ljiljana Pasa-Tolic,Sarah M Williams,Mary Lipton,Steven J Hallam,Ryan M Ziels","doi":"10.1038/s41564-025-02146-w","DOIUrl":"https://doi.org/10.1038/s41564-025-02146-w","url":null,"abstract":"Syntrophic microbial consortia can contribute substantially to the activity of anoxic ecosystems but are often too rare to allow the study of their in situ physiologies using traditional molecular methods. Here we combined bioorthogonal non-canonical amino acid tagging (BONCAT), stable isotope probing and metaproteomics to improve the recovery of proteins from active members and track isotope incorporation in an anaerobic digestion community. Click-chemistry-enabled cell sorting and direct protein pull-down coupled to metaproteomics improved recovery of isotopically labelled proteins during anaerobic acetate oxidation. BONCAT-enabled protein profiles revealed elevated activity and labelling of a rare and so-far uncharacterized syntrophic bacterium belonging to the family Natronincolaceae that expressed a previously hypothesized oxidative glycine pathway for syntrophic acetate oxidation. Stable-isotope-probing-informed metabolic modelling predicted that this organism accounted for a majority of acetate flux, suggesting that the oxidative glycine pathway is an important route for anaerobic carbon transformation and is probably central to community metabolism in natural and engineered ecosystems.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"208-209 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145338588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Metabolic remodelling produces fumarate via the aspartate-argininosuccinate shunt in macrophages as an antiviral defence. 作者更正：代谢重塑通过巨噬细胞中的天冬氨酸-精氨酸琥珀酸分流产生富马酸，作为抗病毒防御。

IF 19.4 1区生物学

Nature Microbiology Pub Date : 2025-10-20 DOI: 10.1038/s41564-025-02188-0

Wenjun Xia, Youxiang Mao, Ziyan Xia, Jie Cheng, Peng Jiang

引用次数: 0

Archaeal symbioses all the way down. 古细菌的共生关系。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-20 DOI: 10.1038/s41564-025-02158-6

Mike Dyall-Smith

引用次数: 0

Viruses and virus satellites of haloarchaea and their nanosized DPANN symbionts reveal intricate nested interactions. 盐古菌的病毒和病毒卫星及其纳米级的DPANN共生体揭示了复杂的嵌套相互作用。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-17 DOI: 10.1038/s41564-025-02149-7

Yifan Zhou,Ana Gutiérrez-Preciado,Ying Liu,David Moreira,Michail M Yakimov,Purificación López-García,Mart Krupovic

{"title":"Viruses and virus satellites of haloarchaea and their nanosized DPANN symbionts reveal intricate nested interactions.","authors":"Yifan Zhou,Ana Gutiérrez-Preciado,Ying Liu,David Moreira,Michail M Yakimov,Purificación López-García,Mart Krupovic","doi":"10.1038/s41564-025-02149-7","DOIUrl":"https://doi.org/10.1038/s41564-025-02149-7","url":null,"abstract":"Nested symbioses, including hyperparasitism in which parasites exploit other parasites within a host, are common in nature. However, such nested interactions remain poorly studied in archaea. Here we characterize this phenomenon in ultra-small archaea of the candidate phylum Nanohaloarchaeota, members of the DPANN superphylum (named after its first representative phyla: Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota and Nanohaloarchaeota) that form obligate interactions with halophilic archaea of the class Halobacteria. We reconstructed the viromes from geothermally influenced salt lakes in the Danakil Depression, Ethiopia, and find that nanohaloarchaea and haloarchaea are both associated with head-tailed, tailless icosahedral, pleomorphic and spindle-shaped viruses, representing 16 different families. These viruses exhibit convergent adaptation to hypersaline environments, encode diverse auxiliary metabolic genes and exchange genes horizontally with each other. We further characterize plasmid-derived satellites that independently evolved to parasitize spindle-shaped viruses of haloarchaea and nanohaloarchaea, revealing another layer of nested symbiosis. Collectively, our findings highlight the complexity of virus-host and virus-virus interactions in hypersaline environments.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"20 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145311248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivating intracellular bacterial persisters to boost antibiotic effectiveness. 重新激活细胞内的细菌，以提高抗生素的有效性。

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-10-17 DOI: 10.1038/s41564-025-02136-y

引用次数: 0