Nature Microbiology最新文献

HIV-1 capsid found guilty of breaking and entering HIV-1衣壳因擅闯民宅被判有罪

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-31 DOI: 10.1038/s41564-025-02071-y

Adarsh Dharan, Edward M. Campbell

引用次数: 0

Polypeptides synthesized by common bacteria in the human gut improve rodent metabolism 人类肠道中常见细菌合成的多肽可改善啮齿动物的新陈代谢

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-31 DOI: 10.1038/s41564-025-02064-x

Yong Fan, Liwei Lyu, Ruben Vazquez-Uribe, Wanliang Zhang, Mareike Bongers, Andreas Koulouktsis, Mengliu Yang, Vita Sereika-Bejder, Tulika Arora, Evelina Stankevic, Jeremy Armetta, Franziska Zosel, Charlotta D. de la Cour, Lotte Simonsen, Alina Kulakova, Michael Wierer, Pernille Harris, Joachim Gæde, Peter Rossing, Filip K. Knop, Tune H. Pers, Tue Haldor Hansen, Trine Nielsen, Ling Li, Kristian Strømgaard, Gangyi Yang, Morten Otto Alexander Sommer, Oluf Pedersen

{"title":"Polypeptides synthesized by common bacteria in the human gut improve rodent metabolism","authors":"Yong Fan, Liwei Lyu, Ruben Vazquez-Uribe, Wanliang Zhang, Mareike Bongers, Andreas Koulouktsis, Mengliu Yang, Vita Sereika-Bejder, Tulika Arora, Evelina Stankevic, Jeremy Armetta, Franziska Zosel, Charlotta D. de la Cour, Lotte Simonsen, Alina Kulakova, Michael Wierer, Pernille Harris, Joachim Gæde, Peter Rossing, Filip K. Knop, Tune H. Pers, Tue Haldor Hansen, Trine Nielsen, Ling Li, Kristian Strømgaard, Gangyi Yang, Morten Otto Alexander Sommer, Oluf Pedersen","doi":"10.1038/s41564-025-02064-x","DOIUrl":"https://doi.org/10.1038/s41564-025-02064-x","url":null,"abstract":"The human gut microbiota has the potential to synthesize proteins that may influence host metabolism. Here we report two polypeptides, RUMTOR-derived peptide (RORDEP) 1 and RORDEP2, circulating in human blood and synthesized by specific strains of gut commensal Ruminococcus torques that correlate inversely with adiposity in humans. Oral gavage with RORDEP-expressing strains improved glucose tolerance, increased bone density and reduced fat mass with an enhanced expression of genes and proteins involved in thermogenesis and lipolysis in lean mice on a high-fat diet and diet-induced obese mice. Recombinant RORDEP1 given to rats intraperitoneally decreased plasma gastric inhibitory polypeptide but increased glucagon-like peptide 1, peptide YY and insulin. Intestinal delivery of recombinant RORDEP1 to rats potentiated insulin-mediated inhibition of hepatic glucose production by downregulating genes and proteins controlling liver gluconeogenesis, glycogenolysis and lipogenesis but upregulating those involved in insulin signalling, glycogenesis and glycolysis. These preclinical findings warrant the exploration of RORDEPs for the prevention and treatment of human metabolic disorders.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"13 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From farm to factory to fork 从农场到工厂再到餐桌

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-31 DOI: 10.1038/s41564-025-02069-6

Kate S. Baker, Alison E. Mather

引用次数: 0

Deep, long-read metagenome sequencing expands knowledge of prokaryotic phylogenetic diversity 深度、长读元基因组测序扩展了对原核生物系统发育多样性的认识

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-31 DOI: 10.1038/s41564-025-02073-w

引用次数: 0

The food-associated resistome is shaped by processing and production environments 与食物相关的抵抗组受加工和生产环境的影响

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-30 DOI: 10.1038/s41564-025-02059-8

Narciso M. Quijada, José F. Cobo-Díaz, Vincenzo Valentino, Coral Barcenilla, Francesca De Filippis, Raul Cabrera-Rubio, Niccolò Carlino, Federica Pinto, Monika Dzieciol, Inés Calvete-Torre, Carlos Sabater, Francesco Rubino, Stephen Knobloch, Sigurlaug Skirnisdottir, Lorena Ruiz, Mercedes López, Miguel Prieto, Viggó Thór Marteinsson, Abelardo Margolles, Nicola Segata, Paul D. Cotter, Martin Wagner, Danilo Ercolini, Avelino Alvarez-Ordóñez

{"title":"The food-associated resistome is shaped by processing and production environments","authors":"Narciso M. Quijada, José F. Cobo-Díaz, Vincenzo Valentino, Coral Barcenilla, Francesca De Filippis, Raul Cabrera-Rubio, Niccolò Carlino, Federica Pinto, Monika Dzieciol, Inés Calvete-Torre, Carlos Sabater, Francesco Rubino, Stephen Knobloch, Sigurlaug Skirnisdottir, Lorena Ruiz, Mercedes López, Miguel Prieto, Viggó Thór Marteinsson, Abelardo Margolles, Nicola Segata, Paul D. Cotter, Martin Wagner, Danilo Ercolini, Avelino Alvarez-Ordóñez","doi":"10.1038/s41564-025-02059-8","DOIUrl":"https://doi.org/10.1038/s41564-025-02059-8","url":null,"abstract":"Food production systems may act as transmission routes for antimicrobial-resistant (AMR) bacteria and AMR genes (AMRGs) to humans. However, the food resistome remains poorly characterized. Here 1,780 raw-material (milk, brine, fresh meat and so on), end-product (cheese, fish, meat products and vegetables) and surface (processing, cooling, smoking, ripening and packing rooms) samples from 113 food processing facilities were subjected to whole-metagenome sequencing. Assembly-free analyses demonstrated that >70% of all known AMRGs, including many predicted to confer resistance to critically important antibiotics, circulate throughout food production chains, with those conferring resistance to tetracyclines, β-lactams, aminoglycosides and macrolides being the most abundant overall. An assembly-based analysis highlighted that bacteria from the ESKAPEE group, together with Staphylococcus equorum and Acinetobacter johnsonii, were the main AMRG carriers. Further evaluation demonstrated that ~40% of the AMRGs were associated with mobile genetic elements, mainly plasmids. These findings will help guide the appropriate use of biocides and other antimicrobials in food production settings when designing efficient antimicrobial stewardship policies.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"12 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-proteomic profiling of the varicella-zoster virus–host interface reveals host susceptibilities to severe infection 水痘-带状疱疹病毒-宿主界面的多蛋白质组学分析揭示了宿主对严重感染的易感性

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-30 DOI: 10.1038/s41564-025-02068-7

Virginie Girault, Alexey Stukalov, Madalina Elena Carter-Timofte, Jonny Hertzog, Melissa Verin, Katharina Austen, Darya A. Haas, Lila Oubraham, Antonio Piras, Susanne Maidl, Rupert Öllinger, Roland Rad, Ulrike Protzer, Benedikt B. Kaufer, Robert J. Lebbink, Jan Rehwinkel, Trine H. Mogensen, Andreas Pichlmair

{"title":"Multi-proteomic profiling of the varicella-zoster virus–host interface reveals host susceptibilities to severe infection","authors":"Virginie Girault, Alexey Stukalov, Madalina Elena Carter-Timofte, Jonny Hertzog, Melissa Verin, Katharina Austen, Darya A. Haas, Lila Oubraham, Antonio Piras, Susanne Maidl, Rupert Öllinger, Roland Rad, Ulrike Protzer, Benedikt B. Kaufer, Robert J. Lebbink, Jan Rehwinkel, Trine H. Mogensen, Andreas Pichlmair","doi":"10.1038/s41564-025-02068-7","DOIUrl":"https://doi.org/10.1038/s41564-025-02068-7","url":null,"abstract":"Varicella-zoster virus (VZV) infects most humans and causes chickenpox, shingles and central nervous system pathologies. The molecular basis for these phenotypes remains elusive. Here we conducted a multi-proteomic survey on 64 individual VZV proteins and infection-induced perturbations in a neuronal cell line, identifying 900 interactors and 3,618 regulated host proteins. Data integration suggested molecular functions of viral proteins, such as a mechanism for the ORF61-mediated IFI16 degradation via the recruitment of E3 ligase co-factors. Moreover, we identified proviral host factors (MPP8 and ZNF280D) as potential targets to limit infection. Integration of exome sequencing analysis from patients with VZV-associated central nervous system pathologies identified nephrocystin 4 as a viral restriction factor, and its S862N variant, which showed reduced activity and decreased binding to the regulatory proteins 14-3-3. Collectively, our study provides a comprehensive herpesvirus–host interface resource, which aids our understanding of disease-associated molecular perturbations and data-driven identification of antiviral treatment options.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"26 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria 在革兰氏阴性菌中，肽聚糖-外膜附着产生质周压力以防止裂解

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-29 DOI: 10.1038/s41564-025-02058-9

Michaël Deghelt, Seung-Hyun Cho, Jiawei Sun, Sander K. Govers, Arne Janssens, Alix Vincent Dachsbeck, Han K. Remaut, Kerwyn Casey Huang, Jean-François Collet

{"title":"Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria","authors":"Michaël Deghelt, Seung-Hyun Cho, Jiawei Sun, Sander K. Govers, Arne Janssens, Alix Vincent Dachsbeck, Han K. Remaut, Kerwyn Casey Huang, Jean-François Collet","doi":"10.1038/s41564-025-02058-9","DOIUrl":"https://doi.org/10.1038/s41564-025-02058-9","url":null,"abstract":"Bacteria are subject to a substantial concentration differential of osmolytes between the interior and exterior of the cell, resulting in turgor pressure. Failure to mechanically balance this turgor pressure causes cells to burst. Here, using microfluidics, imaging, biochemistry and mathematical modelling, we analysed how Escherichia coli cells with structural mutations in the envelope respond to hypoosmotic shocks. We show that the peptidoglycan cell wall forms a mechanical unit with the outer membrane that limits periplasmic volume increase under hypoosmotic shock, allowing osmotic pressure build-up in the periplasm. In turn, this periplasmic pressure balances cytoplasmic turgor across the inner membrane, preventing cell lysis and death. Thus, while the peptidoglycan layer is necessary, it is not sufficient to maintain turgor and protect cells from lysis. We propose a model in which the entire cell envelope, including the periplasm, collectively enables Gram-negative bacteria to overcome osmotic challenges.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"23 1","pages":""},"PeriodicalIF":28.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and opportunities in mRNA vaccine development against bacteria 细菌mRNA疫苗开发的挑战和机遇

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-29 DOI: 10.1038/s41564-025-02070-z

Ilke Aernout, Rein Verbeke, Fabien Thery, Patrick Willems, Uri Elia, Stefaan C. De Smedt, Rino Rappuoli, Dan Peer, Francis Impens, Ine Lentacker

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Evolution of antifungal resistance in the environment 环境中抗真菌耐药性的进化

IF 28.3 1区生物学

Nature Microbiology Pub Date : 2025-07-29 DOI: 10.1038/s41564-025-02055-y

Norman van Rhijn, Johanna Rhodes

引用次数: 0

Remote control designer probiotics 遥控设计益生菌