Mycorrhiza最新文献

{"title":"Fungi in hair roots of Vaccinium spp. (Ericaceae) growing on decomposing wood: colonization patterns, identity, and in vitro symbiotic potential.","authors":"Martin Vohník, Martina Réblová","doi":"10.1007/s00572-023-01101-z","DOIUrl":"10.1007/s00572-023-01101-z","url":null,"abstract":"<p><p>Most of our knowledge on the ericoid mycorrhizal (ErM) symbiosis comes from temperate heathlands characterized by acidic peaty soils and many experiments with a few ascomycetous fungi. However, ericaceous plants thrive in many other ecosystems and in temperate coniferous forests, their seedlings often prosper on decomposing wood. While wood is typically exploited by basidiomycetous ectomycorrhizal (EcM) and saprobic fungi, the role of ErM fungi (ErMF) is much less clear. We explored the cultivable mycobiota of surface sterilized hair roots of Vaccinium spp. growing on decomposing wood in two coniferous forests in Mid-Norway (Scandinavia) and Northern Bohemia (Central Europe). Obtained isolates were identified using molecular tools and their symbiotic potential was tested in vitro. While the detected community lacked the archetypal ErMF Hyaloscypha hepaticicola and the incidence of dark septate endophytes and EcM fungi was negligible, it comprised other frequent asexual ascomycetous ErMF, namely H. variabilis and Oidiodendron maius, together with several isolates displaying affinities to sexual saprobic H. daedaleae and H. fuckelii. Ascomycete-suppressing media revealed representatives of the saprobic basidiomycetous genera Coprinellus, Gymnopilus, Mycena (Agaricales), and Hypochnicium (Polyporales). In the resyntheses, the tested basidiomycetes occasionally penetrated the rhizodermal cells of their hosts but never formed ericoid mycorrhizae and in many cases overgrew and killed the inoculated seedlings. In contrast, a representative of the H. daedaleae/H. fuckelii-related isolates repeatedly formed what morphologically appears as the ErM symbiosis and supported host's growth. In conclusion, while basidiomycetous saprobic fungi have a potential to colonize healthy-looking ericaceous hair roots, the mode(-s) of their functioning remain obscure. For the first time, a lineage in Hyaloscypha s. str. (corresponding to the former Hymenoscyphus ericae aggregate) where sexual saprobes are intermingled with root symbionts has been revealed, shedding new light on the ecology and evolution of these prominent ascomycetous ErMF.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"33 1-2","pages":"69-86"},"PeriodicalIF":3.3,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9299181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Islands in the shade: scattered ectomycorrhizal trees influence soil inoculum and heterospecific seedling response in a northeastern secondary forest.","authors":"Andrew M Cortese,&nbsp;Thomas R Horton","doi":"10.1007/s00572-023-01104-w","DOIUrl":"https://doi.org/10.1007/s00572-023-01104-w","url":null,"abstract":"<p><p>The eastern deciduous forest is a mix of arbuscular (AM) and ectomycorrhizal (EM) trees, but land use legacies have increased the abundance of AM trees like Acer spp. (maple). Although these legacies have not changed the abundance of some EM trees like Betula spp. (birch), EM conifers like Tsuga canadensis (hemlock), and Pinus strobus (pine) have declined. We used a soil bioassay to investigate if the microbial community near EM birch (birch soil) contains a greater abundance and diversity of EM fungal propagules compatible with T. canadensis and P. strobus compared to the community associated with the surrounding AM-dominated secondary forest matrix (maple soil). We also tested the effectiveness of inoculation with soil from a nearby EM-dominated old-growth forest as a restoration tool to reintroduce EM fungi into secondary forest soils. Finally, we examined how seedling growth responded to EM fungi associated with each treatment. Seedlings grown with birch soil were colonized by EM fungi mostly absent from the surrounding maple forest. Hemlock seedlings grown with birch soil grew larger than hemlock seedlings grown with maple soil, but pine seedling growth did not differ with soil treatment. The addition of old-growth soil inoculum increased hemlock and pine growth in both soils. Our results found that EM trees are associated with beneficial EM fungi that are mostly absent from the surrounding AM-dominated secondary forest, but inoculation with old-growth soil is effective in promoting the growth of seedlings by reintroducing native EM fungi to the AM-dominated forests.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"33 1-2","pages":"33-44"},"PeriodicalIF":3.9,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10751168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizophagus irregularis improves Hg tolerance of Medicago truncatula by upregulating the Zn transporter genes ZIP2 and ZIP6.","authors":"Yaqin Guo,&nbsp;Nadine Sommer,&nbsp;Konrad Martin,&nbsp;Frank Rasche","doi":"10.1007/s00572-022-01100-6","DOIUrl":"https://doi.org/10.1007/s00572-022-01100-6","url":null,"abstract":"<p><p>Mercury (Hg) pollution of soils is a critical environmental problem. To rehabilitate Hg contaminated soils, arbuscular mycorrhizal (AM) fungi-based phytoremediation may be supportive, yet the functional potential of AM fungi in response to Hg exposure is unclear. In a greenhouse experiment, we assessed the response of Medicago truncatula (Hg tolerance index (TI), Hg partitioning) to different Hg concentrations [0 (Hg0), 25 (Hg25), 50 (Hg50) µg g^-1] in treatments with (AM) and without (NM) inoculation of Rhizophagus irregularis. Additionally, zinc (Zn) uptake and the expression of two Zn transporter genes (ZIP2, ZIP6) were examined because Zn is an essential element for plants and shares the same outer electronic configuration as Hg, implying potential competition for the same transporters. The results showed that AM plants had a higher TI than NM plants. Plant roots were identified as dominant Hg reservoirs. AM inoculation reduced the root Hg concentration under Hg50 compared to the NM treatment. There was an interaction between Hg treatment and AM inoculation on Hg stem concentration, i.e., at Hg25, AM inoculation decreased the Hg translocation from roots to stems, while Hg translocation was increased at Hg50 compared to the NM treatment. Zn acquisition was improved by R. irregularis. The negative relationship between Hg and Zn concentrations in the roots of AM and NM plants implied potential competition for the same transporters, although the expression of Zn transporters was upregulated by AM inoculation at all Hg levels. In conclusion, this baseline study demonstrated that R. irregularis may play an important role in Hg tolerance of M. truncatula, suggesting its potential for Hg-contaminated phytoremediation.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"33 1-2","pages":"23-32"},"PeriodicalIF":3.9,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10750171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-occurring epiphytic orchids have specialized mycorrhizal fungal niches that are also linked to ontogeny.","authors":"Melania Fernández,&nbsp;Jaspreet Kaur,&nbsp;Jyotsna Sharma","doi":"10.1007/s00572-022-01099-w","DOIUrl":"https://doi.org/10.1007/s00572-022-01099-w","url":null,"abstract":"<p><p>Mycorrhizal symbiosis has been related to the coexistence and community assembly of coexisting orchids in few studies despite their obligate dependence on mycorrhizal partners to establish and survive. In hyper-diverse environments like tropical rain forests, coexistence of epiphytic orchids may be facilitated through mycorrhizal fungal specialization (i.e., sets of unique and dominant mycorrhizal fungi associated with a particular host species). However, information on the role of orchid mycorrhizal fungi (OMF) in niche differentiation and coexistence of epiphytic orchids is still scarce. In this study, we sought to identify the variation in fungal preferences of four co-occurring epiphytic orchids in a tropical rainforest in Costa Rica by addressing the identity and composition of their endophytic fungal and OMF communities across species and life stages. We show that the endophytic fungal communities are formed mainly of previously recognized OMF taxa, and that the four coexisting orchid species have both a set of shared mycorrhizal fungi and a group of fungi unique to an orchid species. We also found that adult plants keep the OMF of the juvenile stage while adding new mycobionts over time. This study provides evidence for the utilization of specific OMF that may be involved in niche segregation, and for an aggregation mechanism where adult orchids keep initial fungal mycobionts of the juvenile stage while adding others.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"33 1-2","pages":"87-105"},"PeriodicalIF":3.9,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10751452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycorrhizal diversity and community composition in co-occurring Cypripedium species.","authors":"Huanchu Liu,&nbsp;Hans Jacquemyn,&nbsp;Shuai Yu,&nbsp;Wei Chen,&nbsp;Xingyuan He,&nbsp;Yanqing Huang","doi":"10.1007/s00572-022-01095-0","DOIUrl":"https://doi.org/10.1007/s00572-022-01095-0","url":null,"abstract":"<p><p>Orchids commonly rely on mycorrhizal fungi to obtain the necessary resources for seed germination and growth. Whereas most photosynthetic orchids typically associate with so-called rhizoctonia fungi to complete their life cycle, there is increasing evidence that other fungi may be involved as well and that the mycorrhizal communities associated with orchids may be more diverse. Coexisting orchid species also tend to associate with different fungi to reduce competition for similar resources and to increase long-term population viability. However, few studies have related the mycorrhizal communities in the rhizosphere to communities found in the roots of closely related coexisting orchid species. In this study, we used high-throughput sequencing to investigate the diversity and community composition of orchid mycorrhizal fungi in the roots and the rhizosphere of four Cypripedium species growing in forests in Northeast China. The results showed that the investigated Cypripedium species associated with a wide variety of fungi including members of Tulasnellaceae, Psathyrellaceae, and Herpotrichiellaceae, whereas members of Russulaceae, Cortinariaceae, Thelephoraceae, and Herpotrichiellaceae showed high abundance in rhizosphere soils. The diversity of fungi detected in the rhizosphere soil was much higher than that in the roots. The observed variation in fungal communities in Cypripedium roots was not related to forest site or orchid species. On the other hand, variation in mycorrhizal communities of rhizosphere soil was significantly related to sampling site. These results indicate that orchid mycorrhizal communities in the rhizosphere display considerable variation among sites and that orchids use only a subset of the locally available fungi. Future studies focusing on the fine-scale spatial distribution of orchid mycorrhizal fungi and more detailed assessments of local environmental conditions will provide novel insights into the mechanisms explaining variation of fungal communities in both orchid roots and the rhizosphere.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"33 1-2","pages":"107-118"},"PeriodicalIF":3.9,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9313563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Referee acknowledgement","authors":"Not Available Not Available","doi":"10.1007/s005720100101","DOIUrl":"https://doi.org/10.1007/s005720100101","url":null,"abstract":"","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"10 1","pages":"307"},"PeriodicalIF":3.9,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s005720100101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47234026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome analyses based on single, field collected spores of the arbuscular mycorrhizal fungus Funneliformis geosporum.","authors":"Shadi Eshghi Sahraei, Marisol Sánchez-García, Merce Montoliu-Nerin, David Manyara, Claudia Bergin, Søren Rosendahl, Anna Rosling","doi":"10.1007/s00572-022-01091-4","DOIUrl":"10.1007/s00572-022-01091-4","url":null,"abstract":"<p><p>Arbuscular mycorrhizal (AM) fungi are ubiquitous mutualistic symbionts of most terrestrial plants and many complete their lifecycles underground. Whole genome analysis of AM fungi has long been restricted to species and strains that can be maintained under controlled conditions that facilitate collection of biological samples. There is some evidence suggesting that AM fungi can adapt to culture resulting in phenotypic and possibly also genotypic changes in the fungi. In this study, we used field isolated spores of AM fungi and identified them as Funneliformis geosporum based on morphology and phylogenetic analyses. We separately assembled the genomes of two representative spores using DNA sequences of 19 and 22 individually amplified nuclei. The genomes were compared with previously published data from other members of Glomeraceae including two strains of F. mosseae. No significant differences were observed among the species in terms of gene content, while the single nucleotide polymorphism density was higher in the strains of F. geosporum than in the strains of F. mosseae. In this study, we demonstrate that it is possible to sequence and assemble genomes from AM fungal spores sampled in the field, which opens up the possibility to include uncultured AM fungi in phylogenomic and comparative genomic analysis and to study genomic variation in natural populations of these important plant symbionts.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"32 5-6","pages":"361-371"},"PeriodicalIF":3.3,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9560946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33485509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Native ectomycorrhizal fungi from the endangered pine rocklands are superior symbionts to commercial inoculum for slash pine seedlings.","authors":"Elena Karlsen-Ayala,&nbsp;Matthew E Smith,&nbsp;Bryce C Askey,&nbsp;Romina Gazis","doi":"10.1007/s00572-022-01092-3","DOIUrl":"https://doi.org/10.1007/s00572-022-01092-3","url":null,"abstract":"<p><p>The south Florida pine rocklands is a critically endangered, fire-dependent ecosystem dominated by the overstory tree Pinus densa (South Florida slash pine). Because pine recruitment in this ecosystem has proven problematic, restoration efforts need to include replanting slash pine trees. Even though ectomycorrhizal fungi are known to be critical symbionts of young pines and are necessary for the development of healthy pines, virtually nothing is known about these mutualists and their role in pine establishment and survival in the pine rocklands. One approach to improve pine establishment is to grow seedlings in a nursery before outplanting, facilitating early associations with ectomycorrhizae, and therefore improving seedling health. In this study, we compared health metrics (height, stem diameter, final needle length, root length, root colonization, needle greenness, root volume, and root:shoot ratio) of seedlings grown in soil amended with five commercially available mycorrhizal inocula versus field soil collected from three pine rockland fragments. Seedlings grown with native field soil from the pine rocklands generally performed better than those grown with commercial inoculum in all metrics except root length. According to their labels, each commercial inoculum contained between 4 and 10 ectomycorrhizal fungi species. However, no ectomycorrhizal fungi were recovered from two of the inoculum products and only three ectomycorrhizal fungi in total were recovered from the other three products. In contrast, seedlings grown with field soil are associated with ten ectomycorrhizal species. Our results highlight the importance of incorporating native ectomycorrhizal fungi into pine seedling replanting as part of restoration efforts in the pine rocklands.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"32 5-6","pages":"465-480"},"PeriodicalIF":3.9,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33494125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hedgerows increase the diversity and modify the composition of arbuscular mycorrhizal fungi in Mediterranean agricultural landscapes.","authors":"Guillermo González Fradejas,&nbsp;David García de León,&nbsp;Martti Vasar,&nbsp;Kadri Koorem,&nbsp;Martin Zobel,&nbsp;Maarja Öpik,&nbsp;Mari Moora,&nbsp;José María Rey Benayas","doi":"10.1007/s00572-022-01090-5","DOIUrl":"https://doi.org/10.1007/s00572-022-01090-5","url":null,"abstract":"<p><p>Sustainable agriculture is essential to address global challenges such as climate change and biodiversity loss. Hedgerows enhance aboveground biodiversity and provide ecosystem services, but little is known about their impact on soil biota. Arbuscular mycorrhizal (AM) fungi are one of the key components of belowground biodiversity. We compared the diversity and composition of AM fungal communities at four farmland sites located in Central Spain, where 132 soil samples in total were collected to assess soil physical and chemical properties and the AM fungal communities. We compared the richness (number of AM fungal taxa), taxonomic, functional, and phylogenetic diversity, and structure of the AM fungal communities across three farmland habitat types, namely hedgerows, woody crops (olive groves and vineyard), and herbaceous crops (barley, sunflower, and wheat). Our results showed positive effects of hedgerows on most diversity metrics. Almost 60% of the AM fungal taxa were shared among the three farmland habitat types. Hedgerows increased AM fungal taxonomic richness (31%) and alpha diversity (25%), and especially so compared to herbaceous crops (45% and 28%, respectively). Hedgerows harbored elevated proportions of AM fungi with non-ruderal life-history strategies. AM fungal communities were more similar between hedgerows and woody crops than between hedgerows and adjacent herbaceous crops, possibly because of differences in tillage and fertilization. Unexpectedly, hedgerows reduced phylogenetic diversity, which might be related to more selective associations of AM fungi with woody plants than with herbaceous crops. Overall, the results suggest that planting hedgerows contributes to maintain belowground diversity. Thus, European farmers should plant more hedgerows to attain the goals of the EU Biodiversity Strategy for 2030.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"32 5-6","pages":"397-407"},"PeriodicalIF":3.9,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9561024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33457958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental response of arbuscular mycorrhizal fungi under soybean cultivation at a regional scale.","authors":"Valeria Soledad Faggioli, Fernanda Covacevich, Gabriel Grilli, Claudio Lorenzon, Bethania Aimetta, Monica Sagadin, Adrián Langarica-Fuentes, Marta Noemí Cabello","doi":"10.1007/s00572-022-01093-2","DOIUrl":"10.1007/s00572-022-01093-2","url":null,"abstract":"<p><p>Climate change, the shortage of fertilizers and reduced land for cultivation have drawn attention to the potential aid provided by soil-borne organisms. Arbuscular mycorrhizal fungi (AMF) offer a wide range of ecosystem benefits and hence, understanding the mechanisms that control AMF occurrence and maintenance is essential for resilient crop production. We conducted a survey of 123 soybean fields located across a 75,000-km² area of Argentina to explore AMF community composition and to quantify the impact of soil, climate, and geographical distance on these key soil organisms. First, based upon morphological identification of spores, we compiled a list of the AMF species found in the studied area and identified Acaulospora scrobiculata and Glomus fuegianum as the most frequent species. G. fuegianum abundance was negatively correlated with precipitation seasonality and positively correlated with mean annual precipitation as well as mycorrhizal colonisation of soybean roots. Second, we observed that species richness was negatively correlated with soil P availability (Bray I), clay content and mean annual precipitation. Finally, based on partitioning variation analysis, we found that AMF exhibited spatial patterning at a broad scale. Therefore, we infer that geographical distance was positively associated with spore community composition heterogeneity across the region. Nevertheless, we highlight the importance of precipitation sensitivity of frequent species, overall AMF richness and community composition, revealing a crucial challenge to forthcoming agriculture considering an expected change in global climate patterns.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"32 5-6","pages":"425-438"},"PeriodicalIF":3.9,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33512822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}