Mycorrhiza最新文献_第8页

Competitive dynamics of arbuscular mycorrhizal fungi as depending on fungal traits and host plant species. 丛枝菌根真菌的竞争动态取决于真菌性状和寄主植物种类。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-02-24 DOI: 10.1007/s00572-026-01254-7

Henry J De La Cruz, Nicolás Marro, Milena Caccia, Kateřina Žďárská, Martina Janoušková

{"title":"Competitive dynamics of arbuscular mycorrhizal fungi as depending on fungal traits and host plant species.","authors":"Henry J De La Cruz, Nicolás Marro, Milena Caccia, Kateřina Žďárská, Martina Janoušková","doi":"10.1007/s00572-026-01254-7","DOIUrl":"10.1007/s00572-026-01254-7","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are ubiquitous root-associated symbionts, but competitive interactions among coexisting taxa remain poorly understood. The variation in colonization and resource-acquisition strategies drive competition, shaping the relative abundances of AMF within their communities and mycorrhiza functioning. However, the factors that determine the competitive outcomes have received limited experimental attention. We hypothesized that AMF abundances, competitive responses, and contributions to plant growth would vary according to fungal traits and host plant species. Three AMF isolates of contrasting growth and competitive abilities, each representing one AMF species, were mono- and co-inoculated under six host plant species. Fungal abundance, root colonization (RC), and mycorrhizal growth response (MGR) were measured at early and late stages of the symbiosis.Fungal competitive responses depended on the isolate’s root colonization strategy in monoculture and mycorrhizal stage. The fast-colonizing isolate dominated initially, while slower-growing isolates displayed different temporal patterns, either declining or maintaining their abundance over time. Our findings suggest competitive outcomes among AMF that were asymmetric at early stages but became more symmetric later. The host plant species importantly modulated the dominance of the fast root-colonizer. MGR was positively associated with total AMF abundance and RC, yet the most abundant isolate did not necessarily provide the greatest growth benefits to the host plant. Our results suggest that maintaining a diversity of AMF functional types, rather than introducing a single highly competitive isolate, is favourable to the establishment of stable and efficient plant-AMF associations.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12929356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Origin and cohabitation of arbuscular mycorrhizal fungi matter: forest-derived Gigaspora species are promising candidates for bioinoculant development in forest tree production. 丛枝菌根真菌物质的起源和共生：森林来源的Gigaspora物种是森林树木生产中生物接种剂开发的有希望的候选者。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-02-18 DOI: 10.1007/s00572-026-01253-8

Akotchiffor Kevin Geoffroy Djotan, Yosuke Matsuda, Norihisa Matsushita, Kenji Fukuda

{"title":"Origin and cohabitation of arbuscular mycorrhizal fungi matter: forest-derived Gigaspora species are promising candidates for bioinoculant development in forest tree production.","authors":"Akotchiffor Kevin Geoffroy Djotan, Yosuke Matsuda, Norihisa Matsushita, Kenji Fukuda","doi":"10.1007/s00572-026-01253-8","DOIUrl":"10.1007/s00572-026-01253-8","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are potential bioinoculants to grow healthy plants in healthy soils. Although they are generalist, plant – AMF associations work along a mutualism-to-parasitism continuum where currently unresolved host – symbiont compatibility overrides the versatility of AMF functions. Here, we hypothesized that habitat, origin, and cohabitation of AMF determine compatibility and functionality in the associations. To test the hypothesis, we established two forest-inhabiting AMF isolates under Cryptomeria japonica (Cupressaceae) and inoculated them into C. japonica seedlings grown in a controlled environment. We identified the isolates as Gigaspora rosea LFB-4 and G. margarita LFB-A1 using morphological, molecular, and phylogenetic analyses. They simultaneously developed multiple germ tubes during germination, showed pre-symbiotic sporulation, erratic root colonization, and produced spores inside host root cells. When compared to controls, G. rosea and G. margarita significantly promoted the growth of C. japonica seedlings, with synergistic effects in their cohabitation. While G. rosea boosted water uptake and height growth, G. margarita improved biomass production. Together, they encouraged carbon release into the soil and delayed root growth, increasing shoot-to-root biomass ratio for faster seedling growth. We concluded that, despite erratic root colonization, forest-derived G. rosea and G. margarita worked synergistically to improve the growth of C. japonica seedlings by modulating root development. We proved that beyond taxonomic affiliation, habitat, origin, and cohabitation of AMF matter in plant – AMF compatibility for mutual benefits. Our findings imply that forests are home to potent AMF bioinoculants such as beneficial Gigaspora species and diversity improves plant – AMF associations.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 2","pages":"8"},"PeriodicalIF":4.0,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12916936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of ectomycorrhizal communities in Sardinian cork oak forests: influence of management system, lithological substrate and season. 撒丁岛栓皮栎林外生菌根群落动态：管理制度、岩性基质和季节的影响

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-02-11 DOI: 10.1007/s00572-026-01252-9

Salvatore Seddaiu, Camilla Morittu, Antonio Franceschini, Mirco Iotti, Edoardo Scali, Enrico Lancellotti

{"title":"Dynamics of ectomycorrhizal communities in Sardinian cork oak forests: influence of management system, lithological substrate and season.","authors":"Salvatore Seddaiu, Camilla Morittu, Antonio Franceschini, Mirco Iotti, Edoardo Scali, Enrico Lancellotti","doi":"10.1007/s00572-026-01252-9","DOIUrl":"10.1007/s00572-026-01252-9","url":null,"abstract":"Ectomycorrhizal fungi represent a key component of forest ecosystems, contributing significantly to tree nutrition, stress tolerance, and overall ecosystem resilience. In the Mediterranean region, cork oak (Quercus suber L.) forests, have significant ecological and economic value, and their vitality strongly depends on these belowground mutualistic relationships. Although previous studies have investigated the diversity and function of ectomycorrhizal fungi, several aspects concerning their ecological dynamics remain inadequately understood, particularly in cork oak forests. This study investigates the structural and dynamics of ectomycorrhizal fungal communities in cork oak forests of Sardinia located on granitic, basaltic, and trachytic substrates and subjected to different management practices (natural, grazed, and ploughed). We assess how forest management and seasonal variability interact with lithological conditions to shape community structure and diversity. Three cork oak stands for each lithological substrate (nine in total) were selected in areas where all the three forestry managements were present. Two transects were established in each stand, and soil samples were collected during spring and autumn to assess seasonal variations in the ectomycorrhizal community. In total, 82,345 ectomycorrhizal root tips were morphologically characterized and classified in 167 morphotypes based on morpho-anatomical characteristics. From these, 120 were successfully assigned to distinct Operational Taxonomic Units (OTUs) through internal transcribed spacer (ITS) barcoding. Our results indicate that lithological substrate, management system, and sampling season significantly influence the structure and composition of ectomycorrhizal communities. Notably, ploughing caused a marked reduction in fungal richness, highlighting the sensitivity of these communities to soil disturbance.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"7"},"PeriodicalIF":4.0,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12890973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The core microbiome of the AMF hyphosphere: mineralization mechanisms of key soil mineral elements. AMF土壤的核心微生物群：关键土壤矿质元素的矿化机制

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-02-09 DOI: 10.1007/s00572-026-01251-w

Lin Pan, Baiyan Cai

{"title":"The core microbiome of the AMF hyphosphere: mineralization mechanisms of key soil mineral elements.","authors":"Lin Pan, Baiyan Cai","doi":"10.1007/s00572-026-01251-w","DOIUrl":"10.1007/s00572-026-01251-w","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) lack the enzymatic capacity to directly mineralize many essential soil elements and therefore rely on their hyphosphere core microbiome, a microbial consortium increasingly recognized as the \"second genome\" of AMF. However, the definition, functional mechanisms, and ecological relevance of this core microbiome remain poorly resolved. This review addresses how hyphosphere core microorganisms regulate the mineralization of soil carbon, nitrogen, phosphorus, and sulfur. We first outline the conceptual development of the core microbiome and then, for the first time, propose a five-dimensional screening framework integrating abundance stability and universality, functional stability, dynamic responsiveness, ecological niche specificity, and community supportiveness to identify authentic core members. Using this framework, we synthesize evidence on the mechanisms by which the hyphosphere core microbiome mediates biomineralization, highlighting its role in converting organically bound nutrients into plant-available forms. By integrating the functions of hyphosphere core microorganisms across carbon, nitrogen, phosphorus, and sulfur cycles, this review provides a unified ecological perspective on how the AMF hyphosphere core microbiome drives soil nutrient turnover (Fig. 1). Overall, this framework advances understanding of hyphosphere ecology and offers practical implications for soil ecosystem restoration and sustainable agricultural management.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"6"},"PeriodicalIF":4.0,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical armed allies promote plant resistance to pathogen: The contribution of arbuscular mycorrhizal fungi to an invasive weed. 化学武器同盟促进植物对病原体的抗性：丛枝菌根真菌对入侵杂草的贡献。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-02-09 DOI: 10.1007/s00572-026-01250-x

Shanshan Qi, Zhixiang Shu, Misbah Naz, Ying Li, Ruike Zhang, Guangqian Ren, Deli Zhai, Wei Wei, Weiguo Fu, Zhicong Dai, Daolin Du

{"title":"Chemical armed allies promote plant resistance to pathogen: The contribution of arbuscular mycorrhizal fungi to an invasive weed.","authors":"Shanshan Qi, Zhixiang Shu, Misbah Naz, Ying Li, Ruike Zhang, Guangqian Ren, Deli Zhai, Wei Wei, Weiguo Fu, Zhicong Dai, Daolin Du","doi":"10.1007/s00572-026-01250-x","DOIUrl":"10.1007/s00572-026-01250-x","url":null,"abstract":"Invasive weeds often possess strong resistance to biotic stresses, which causes huge ecological problems. Both plant growth regulators (PGRs) and arbuscular mycorrhizal (AM) fungi contribute to plant growth and resistance. However, their combined interactions in invasive plants' defense remain poorly understood. To address this knowledge gap, the invasive weed Alternanthera philoxeroides was treated with gibberellins (GA) and paclobutrazol (PAC), inoculated with Clariodeoglous etunicatum to test its response to pathogenic fungi. We found that these two PGRs suppressed AM fungi colonization. Both GA and AM fungi significantly promoted aboveground plant growth, while the two PGRs and AM fungi reduced pathogen infection. Metabolite analysis revealed that AM fungi inoculation significantly elevated vanillic acid, gentisic acid, and pomiferin content. Moreover, flavone, organic acid, and amino acids were positively related with plant growth, while jasmonic acid and amino acids were correlated with plant resistance. Our findings provide direct evidence that, through PGRs and metabolites, AM fungi could be \"chemical armed\" and contribute to plant growth and resistance to pathogens. These findings offer new insights into how PGRs and AM fungi modulate metabolites to enhance invasive plants' resistance, which might contribute to understanding the mechanism of plant invasion and weed management in agro-ecosystem.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"5"},"PeriodicalIF":4.0,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Belowground allies, aboveground threats: the vulnerability of the Persian oak (Quercus Brantii Lindl.)- arbuscular mycorrhizal fungi symbiosis in a changing climate. 地下的盟友，地上的威胁：波斯橡树（Quercus Brantii Lindl.）的脆弱性-丛枝菌根真菌在气候变化中的共生。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-01-27 DOI: 10.1007/s00572-025-01240-5

Aziz Yousefi, Iraj Mehregan, Javad Hamedi, Younes Asri, Gulzar Khan, Dirk C Albach

{"title":"Belowground allies, aboveground threats: the vulnerability of the Persian oak (Quercus Brantii Lindl.)- arbuscular mycorrhizal fungi symbiosis in a changing climate.","authors":"Aziz Yousefi, Iraj Mehregan, Javad Hamedi, Younes Asri, Gulzar Khan, Dirk C Albach","doi":"10.1007/s00572-025-01240-5","DOIUrl":"10.1007/s00572-025-01240-5","url":null,"abstract":"Climate change poses a major threat to ecosystems worldwide, including Iran's ecologically important Zagros oak forests. These forests are experiencing accelerating decline due to climate-related stress and intensified human pressures, despite their key role in sustaining regional biodiversity. Soil health and the crucial symbiotic partnership between oak trees and arbuscular mycorrhizal fungi (AMF) are crucial for resilience in drought-prone Mediterranean environments. Due to a lack of comprehensive studies, this research aimed to analyze the root-associated microbiome of Persian oak (Quercus brantii) across western and southwestern Iran, specifically focusing on AMF diversity and their ecological role. Our study employed Illumina high-throughput sequencing of ITS and 18 S rRNA V4 markers of root-associated fungal communities to assess taxonomic composition and diversity of 160 trees across eight different sites. Analyses revealed dominant fungal groups, including key AMF taxa like Glomeraceae and Claroideoglomeraceae, with significant spatial variation in diversity and community structure, likely influenced by regional and abiotic factors. In addition, the findings highlight the important ecological function of the Persian oak canopy in creating a favorable microclimate and the essential symbiotic partnership with AMF for drought tolerance and nutrient uptake. However, our study ultimately concludes that despite this crucial symbiosis, the Zagros oak forests remain highly vulnerable to increasing pressures from agricultural expansion and the escalating impacts of climate change, seasonal wildfires, and declining groundwater levels, which pose significant threats to their long-term survival.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"4"},"PeriodicalIF":4.0,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146053043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic and metabolomic analyses reveal the role of flavonoids in ectomycorrhizal symbiosis. 转录组学和代谢组学分析揭示了黄酮类化合物在外生菌根共生中的作用。

IF 4 2区生物学

Mycorrhiza Pub Date : 2026-01-24 DOI: 10.1007/s00572-026-01248-5

Jinhua Zhang, Junfeng Liang, Yanliu Chen, Mengya An, Xing Li

{"title":"Transcriptomic and metabolomic analyses reveal the role of flavonoids in ectomycorrhizal symbiosis.","authors":"Jinhua Zhang, Junfeng Liang, Yanliu Chen, Mengya An, Xing Li","doi":"10.1007/s00572-026-01248-5","DOIUrl":"10.1007/s00572-026-01248-5","url":null,"abstract":"Flavonoids are major plant secondary metabolites that mediate diverse plant–microbe interactions, including ectomycorrhizal (ECM) symbioses. However, their regulatory roles during ECM development remain poorly understood. Here, we investigated whether inoculation with Suillus bovinus alters flavonoid biosynthesis in Pinus yunnanensis roots and assessed how these flavonoids on fungal growth and gene expression. We applied exogenous flavonoids to S. bovinus mycelia to investigate fungal transcriptional and metabolic responses. Following inoculation, differentially expressed genes in P. yunnanensis roots were significantly enriched in the flavonoid biosynthesis pathway. Key enzyme-coding genes, including PAL, CHS, CHI, F3H, and FLS, were upregulated, and this was associated with increased flavonoid accumulation and enhanced antioxidant capacity. In S. bovinus, exogenous flavonoids promoted mycelial growth and induced metabolic adjustments related to carbohydrate and amino acid utilization. Several small secreted protein-related genes showed transcriptional responses to flavonoid exposure, indicating potential transcriptional modulation, although their specific roles in symbiosis remain unclear. These findings indicate that flavonoids may contribute to reciprocal interactions between host roots and ECM fungi and provide a molecular basis for further investigation.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"3"},"PeriodicalIF":4.0,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arbuscular mycorrhizal fungi mitigate cadmium toxicity in plants: A global meta-analysis. 丛枝菌根真菌减轻植物镉毒性：一项全球荟萃分析。

IF 4 2区生物学

Mycorrhiza Pub Date : 2025-12-27 DOI: 10.1007/s00572-025-01246-z

Zahraddeen Kabir Sani, Tariq Shah, Sulaimon Basiru, Maeline Athenaise Salmon, Nabil Radouane, Jean Legeay, Mohamed Hijri

{"title":"Arbuscular mycorrhizal fungi mitigate cadmium toxicity in plants: A global meta-analysis.","authors":"Zahraddeen Kabir Sani, Tariq Shah, Sulaimon Basiru, Maeline Athenaise Salmon, Nabil Radouane, Jean Legeay, Mohamed Hijri","doi":"10.1007/s00572-025-01246-z","DOIUrl":"10.1007/s00572-025-01246-z","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are known to alleviate cadmium (Cd) toxicity in plants; however, the conditions that maximize their efficiency remain poorly understood. While previous meta-analyses have documented general benefits of AMF in Cd-contaminated soils, none has systematically examined the interactive roles of soil pH, inoculant type, and plant biomass on Cd dynamics within the soil-plant system. Here, we present a comprehensive global meta-analysis (97 studies; >500 observations) using advanced statistical approaches, random-effects modeling, meta-regression, and structural equation modelling, to identify these key boundary conditions. AMF inoculation significantly (p < 0.0001) enhanced plant biomass, root and shoot length, and chlorophyll content, while markedly reducing shoot Cd concentration. Effects on antioxidant enzymes were variable and generally non-significant. Notably, AMF efficiency was strongly context-dependent: benefits were greater in acidic soils, and microbial consortia outperformed single-species inoculants in high-biomass plants by promoting root Cd immobilization. In contrast, total soil Cd concentration was a weak predictor of AMF effectiveness (meta-regression R² ≤ 2.03%), indicating that Cd bioavailability, largely determined by pH, is more critical than total metal load. Overall, our findings provide robust evidence that AMF symbiosis is a key bio-based strategy for mitigating Cd stress in plants. This study highlights soil pH, inoculant composition, and plant biomass as critical determinants of AMF efficiency and offers practical guidance for optimizing AMF-based phytostabilization and remediation in Cd-contaminated agroecosystems.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"2"},"PeriodicalIF":4.0,"publicationDate":"2025-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145846255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drink quickly. Mycorrhizal roots deplete water faster from wet soil. 喝得很快。菌根能更快地耗尽潮湿土壤中的水分。

IF 4 2区生物学

Mycorrhiza Pub Date : 2025-12-23 DOI: 10.1007/s00572-025-01247-y

David Püschel, Jana Rydlová, Radka Sudová, Jan Jansa, Michael Bitterlich

{"title":"Drink quickly. Mycorrhizal roots deplete water faster from wet soil.","authors":"David Püschel, Jana Rydlová, Radka Sudová, Jan Jansa, Michael Bitterlich","doi":"10.1007/s00572-025-01247-y","DOIUrl":"10.1007/s00572-025-01247-y","url":null,"abstract":"The aim of the experiment was to identify whether mycorrhizal (M) and non-mycorrhizal (NM) plants differed in their rates of water depletion from soil, which was initially very wet but became moderately dry over time. In a greenhouse pot experiment, dwarf-tomato plants (Solanum lycopersicum ‘Micro Tom’) were grown in a sterilized substrate. The M plants were inoculated with an isolate of the arbuscular mycorrhizal fungus Funneliformis mosseae ‘BEG95’. Moisture meters permanently installed in each pot recorded soil gravimetric water content (GWC) and allowed controlling soil moisture by pot-specific watering. Water depletion was quantified from these GWC data. We found that immediately after water application calculated to establish 17% GWC in each pot, M plants depleted water faster than did their NM counterparts, resulting in less residual substrate water in M as compared to NM pots. The difference in residual substrate GWC remained significant throughout the entire monitoring period of 44 h, with the mean value of the difference being 0.62% of GWC. The plants in the two treatments differed neither in shoot biomass nor leaf area nor root biomass. The M plants, however, had significantly shorter and thicker roots, whereas NM plants had longer, thinner roots. In line with the expectation that thicker roots exhibit higher hydraulic conductivity, we attribute the faster water depletion in M pots to enhanced ability of mycorrhizal roots to exploit wet substrates, where water availability did not constrain uptake. At later stages, both M and NM plants depleted water at similar rates.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"1"},"PeriodicalIF":4.0,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laccaria bicolor adapts to phosphate deficiency at the developmental, transcriptional and metabolic levels. 双色拉虫在发育、转录和代谢水平上适应磷酸盐缺乏。

IF 4 2区生物学

Mycorrhiza Pub Date : 2025-12-11 DOI: 10.1007/s00572-025-01236-1

Anita Loha, Sami Bouziri, Maria V Aparicio Chacon, Giovanna Ambrosini, Katharina Gutbrod, Peter Dörmann, Minna Kemppainen, Francis Martin, Yves Poirier

{"title":"Laccaria bicolor adapts to phosphate deficiency at the developmental, transcriptional and metabolic levels.","authors":"Anita Loha, Sami Bouziri, Maria V Aparicio Chacon, Giovanna Ambrosini, Katharina Gutbrod, Peter Dörmann, Minna Kemppainen, Francis Martin, Yves Poirier","doi":"10.1007/s00572-025-01236-1","DOIUrl":"10.1007/s00572-025-01236-1","url":null,"abstract":"The amount of soluble inorganic phosphate (Pi) in soils is typically low and limiting plant growth. Roots of trees in several forest ecosystems form association with ectomycorrhizal (ECM) fungi, where fungi forage and supply inorganic nutrients, such as Pi, in exchange for fixed carbon. While adaptations of model fungi, such as Saccharomyces cerevisiae, to Pi deficiency has been extensively studied, much less is known about how mycorrhizal fungi adapt to Pi deficiency. This study aimed to decipher how the free-living ECM Laccaria bicolor mycelium adapts to Pi deficiency. L. bicolor grown for 7 days in medium without Pi showed very low Pi and polyphosphate reserves and displayed less compact colonies with spreading hyphae. Pi deficiency resulted in approximately 1500-2000 genes being up- and down-regulated more than 2-fold compared to mycelium grown with abundant Pi, with most genes partially reverting their expression pattern in cultures spiked with Pi for 24 hours. Numerous genes involved in Pi mobilization from organic sources, such as phosphatases and ribonucleases, were induced by Pi deficiency, as well as genes involved in Pi transport, and such expression patterns correlated with increased enzymatic activities. Pi deficiency also induced the synthesis of the betaine lipid diacylglyceryl-N,N,N-trimethylhomoserine (DGTS). Several genes induced by mycorrhization, such as those encoding protease inhibitors belonging to the mycocypin family and Mycorrhizae-Induced Small Secreted Peptides (MiSSP), were also induced by Pi deficiency. Altogether, this study shows that L. bicolor can robustly respond to Pi deficiency and identifies parallels between these adaptations and those involved in mycorrhization.","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"71"},"PeriodicalIF":4.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12698804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145724866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0