南方医科大学学报杂志最新文献

{"title":"[Effects of <i>Baduanjin</i>, bedside cycle ergometer and balloon-blowing training on pulmonary function recovery following thoracoscopic surgery for lung cancer: a randomized controlled trial].","authors":"Juanjuan Zhang, Shuhuan Yao, Zuyuan Zhao, Xiang Sun, Ziyin Zhu, Li Li, Haitao Wei","doi":"10.12122/j.issn.1673-4254.2026.09.20","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.20","url":null,"abstract":"<p><strong>Objectives: </strong>To systematically compare the effects of <i>Baduanjin</i> exercise, bedside cycle ergometer training, and balloon-blowing training on pulmonary function recovery in patients undergoing thoracoscopic radical resection for lung cancer.</p><p><strong>Methods: </strong>This study is a prospective randomized controlled trial. From January, 2021 to June, 2022, 334 patients undergoing thoracoscopic radical resection for lung cancer were enrolled and randomly assigned to <i>Baduanjin</i> group (108 cases), bedside cycle ergometer training group (109 cases), and balloon-blowing training group (117 cases). All the patients received 12 weeks of assigned intervention. Systematic assessments of lung function, exercise tolerance, dyspnea level, pain intensity, immune function, aerobic capacity, quality of life, and patient engagement were conducted at postoperative day 1 (baseline), 4 weeks, 12 weeks, 6 months, and 1 year postoperatively.</p><p><strong>Results: </strong>At 12 weeks postoperatively, all observed indicators in the 3 groups showed significant improvement compared to the baseline levels (<i>P</i><0.05). <i>Baduanjin</i> exercise resulted in the most pronounced improvements in immune function indicators (CD4⁺/CD8⁺ ratio, IgA, and IgG levels), pain intensity, dyspnea score, quality of life score, and treatment engagement, outperforming the other two trainings (<i>P</i><0.05). The patients in bedside cycle ergometer group showed significantly more obvious improvements in forced expiratory volume in one second (FEV1), 6-min walking distance, and peak oxygen uptake (VO₂peak) than those in balloon-blowing training group (<i>P</i><0.05), but these improvements did not differ significantly from those observed in <i>Baduanjin</i> group (<i>P</i>>0.05). Balloon-blowing training resulted in only limited overall improvements of the measured indicators.</p><p><strong>Conclusions: </strong><i>Baduanjin</i>, bedside cycle ergometer training and balloon-blowing training are all effective rehabilitation methods after video-assisted thoracoscopic radical resection for lung cancer. Personalized rehabilitation choices can be provided for patients based on their rehabilitation needs.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2222-2229"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Causal associations between immune cell phenotypes and Ménière's disease: a bidirectional two-sample Mendelian randomization study and validation in mice].","authors":"Lin Yang, Jiaxiang Yu, Lei Han, Jianyu Dai","doi":"10.12122/j.issn.1673-4254.2026.07.20","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.20","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the causal relationship between 731 immune cell phenotypes and Ménière's disease (MD) using Mendelian randomization (MR) analysis and experimental validation in mice.</p><p><strong>Methods: </strong>Based on GWAS data and with immune cell phenotypes and MD as the exposure and outcome variables respectively, we conducted MR analysis using inverse variance weighting (IVW) supplemented by MR-Egger and weighted median methods. Heterogeneity, pleiotropy and leave-one-out sensitivity analyses were performed. Thirty C57BL/6 mice were randomly divided into control, model and anti-CD20 mAb groups, and in the latter two groups, endolymphatic hydrops models were established by LPS injection behind the ear; treatment with the anti-CD20 mAb was administered via tail vein injection before modeling. Auditory and vestibular functions of the mice were evaluated by auditory brainstem response (ABR), water maze and open field tests. HE staining, immunofluorescence, qPCR, Western blotting, immunohistochemistry and ELISA were used to examine inner ear pathology, expressions of immune markers, TLR4/NF-κB pathway, AQP3, and ZO-1, and serum levels of inflammatory factors.</p><p><strong>Results: </strong>MR analysis identified CD19⁺B cells, CD27⁺IgD⁻CD38⁺B cells and CD3⁺T cells as the major risk factors, and CD39⁺CD4⁺T cells as the core protective factor. Sensitivity analysis confirmed robustness of the results without heterogeneity, pleiotropy or reverse causality. The mouse models of endolymphatic hydrops showed obvious auditory/vestibular dysfunction, endolymphatic hydrops, upregulated CD19, CD27 and CD3 expressions, downregulated CD39, AQP3 and ZO-1 expressions, activated TLR4/NF-κB pathway, and elevated serum levels of inflammatory factors. All these abnormalities were significantly improved in anti-CD20 mAb treatment group.</p><p><strong>Conclusions: </strong>Immune cell phenotype abnormality is causally associated with MD, and B cell activation-mediated immune imbalance contributes to MD via the TLR4/NF-κB pathway, inducing inner ear inflammation and abnormal fluid/barrier function. Anti-CD20 mAb ameliorates MD pathology and dysfunction by depleting activated B cells, which provides new evidence for MD immunotherapy.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1671-1684"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Rapid detection of fluconazole resistance in <i>Candida tropicalis</i> using MALDI-TOF mass spectrometry].","authors":"Jie Hou, Weilin Chen, Liang Peng, Fu Qiao, Mei Kang, Ying Ma","doi":"10.12122/j.issn.1673-4254.2026.07.11","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.11","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the efficacy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based antifungal susceptibility testing (MS-AFST) for rapid detection of fluconazole resistance in <i>Candida tropicalis</i>.</p><p><strong>Methods: </strong><i>C. tropicalis</i> isolates from patients with bloodstream infections at West China Hospital of Sichuan University (2018-2023) were collected and identified by chromogenic culture and MALDI-TOF MS. Using Clinical and Laboratory Standards Institute broth microdilution (BMD) method as the reference standard, we compared the performance of the Sensititre YeastOne chromogenic antifungal susceptibility testing and optimized MS-AFST based on the minimum profile change concentration (MPCC).</p><p><strong>Results: </strong>All the 41 isolates were confirmed as <i>C. tropicalis</i>, which showed an azole resistance rate of 36.59% and a proportion of non-wild-type strain of 68.29% with high cross-resistance to azoles. The categorical agreement (CA) and essential agreement (EA) between Sensititre YeastOne and CLSI BMD were both 100%, and their minimum inhibitory concentrations were highly correlated. The MPCC-based MS-AFST enabled rapid detection of fluconazole-resistant phenotype of <i>C. tropicalis</i> within approximately 3 h, demonstrating a CA of 92.68% and an EA of 90.24% both in comparison with the CLSI BMD reference method and Sensititre YeastOne; very major errordiscrepancy occurred in two strains, and minor errordiscrepancy occurred in one strain.</p><p><strong>Conclusions: </strong>MPCC-based MS-AFST enables rapid, reliable detection of fluconazole resistance in <i>C. tropicalis</i> with good agreement with the reference methods. However, classification errors remain, which should be improved by further technical optimization of this method and exploration of the underlying molecular mechanisms.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1576-1584"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[MIS18BP1 regulates epithelial-mesenchymal transition, proliferation, and migration of renal cancer cells <i>via</i> the PI3K/AKT pathway].","authors":"Baofeng Zhao, Xueying Tan, Xuewen Liang, Yuyao Pan, Jiangshui Yuan, Weiqing Song","doi":"10.12122/j.issn.1673-4254.2026.09.15","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.15","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the regulatory role of MIS18BP1 in epithelial-mesenchymal transition (EMT), proliferation, and migration of renal cancer cells and its molecular mechanism.</p><p><strong>Methods: </strong>TCGA and GEO databases were used to analyze the expression patterns of MIS18BP1 in kidney renal clear cell carcinoma (KIRC), and its differential expression between tumor and adjacent tissues was validated by immunohistochemistry. The diagnostic value of MIS18BP1 and its correlation with clinicopathological parameters and immune infiltration were evaluated. Weighted gene co-expression network analysis (WGCNA) was performed to identify MIS18BP1-related gene modules for functional enrichment analysis. In renal cancer cell lines (786-O and ACHN), the effects of lentivirus-mediated MIS18BP1 knockdown on cell proliferation and migration were evaluated using CCK-8, colony formation, Transwell, and wound healing assays. Western blotting was performed to determine the expression levels of EMT-related proteins and PI3K/AKT pathway-related proteins, and rescue experiments were carried out using 740Y-P (a PI3K activator).</p><p><strong>Results: </strong>MIS18BP1 was significantly overexpressed in KIRC tissues and in 786-O and ACHN cells compared with normal renal epithelial cells (HK-2). MIS18BP1 expression level was positively correlated with TNM stage and pathological grade, and showed good diagnostic performance for KIRC (AUC=0.693-0.885). WGCNA and enrichment analyses indicated that MIS18BP1-related genes were significantly enriched in the PI3K/AKT signaling pathway. In cultured renal cancer cells, MIS18BP1 knockdown significantly inhibited the proliferation, migration, and colony-forming ability of the cells, resulting also in decreased expression of N-cadherin and vimentin, increased expression of E-cadherin, and significantly lowered phosphorylation levels of PI3K and AKT. Treatment with 740Y-P partially reversed the inhibitory effects of MIS18BP1 knockdown on cell proliferation and migration.</p><p><strong>Conclusions: </strong>MIS18BP1 is overexpressed in renal cancer and is associated with unfavorable clinical features. High expression of MIS18BP1 enhances proliferation and migration of renal cancer cells possibly by promoting EMT <i>via</i> activating the PI3K/AKT signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2159-2172"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Ethyl caffeate alleviates dextran sulfate sodium-induced colitis in mice by restoring intestinal epithelial tight junctions <i>via</i> activating the Wnt/β-Catenin pathway].","authors":"Keni Zhang, Longtao Zhang, Yu Zhang, Ju Huang, Qingqing Li, Zhijun Geng, Jianguo Hu, Jing Li","doi":"10.12122/j.issn.1673-4254.2026.09.18","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.18","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of ethyl caffeate (EC) on intestinal barrier function in mice with dextran sulfate sodium (DSS)-induced murine colitis and explore the possible mechanism.</p><p><strong>Methods: </strong>Forty male C57BL/6 mice were randomized into control group, DSS model group, and 3 EC groups treated with EC at 5, 10, or 20 mg/kg. Mouse models of colitis were established bytreatment with 2.5% DSS for 7 days, and EC treatment was administered daily by gavage. Colitis phenotypes were assessed by changes in body weight, disease activity index (DAI), colon length and histology. Immunofluorescence staining and PAS staining were used to examine the changes in expressions of the tight junction proteins ZO-1 and claudin-1 and goblet cells, respectively. The intestinal levels of inflammatory factors were detected by ELISA, RT-qPCR and immunohistochemistry. Network pharmacology combined with Western blotting and Wnt inhibitor (IWR-1) was used to verify the mechanism of EC for ameliorating colitis.</p><p><strong>Results: </strong>Treatment with EC at 10 mg/kg significantly alleviated DSS-induced colitis in the mouse models, reduced intestinal pro-inflammatory cytokines and MPO levels, increased goblet cell counts, and restored ZO-1 and claudin-1 expressions and their membrane localization. Network pharmacology identified Wnt/β‑catenin and tight junction pathways as the core targets of EC, which activated the Wnt/β‑catenin pathway by upregulating WNT3A/β‑catenin, and this effect was blocked by treatment with IWR-1.</p><p><strong>Conclusions: </strong>EC ameliorates DSS-induced colitis in mice by activating Wnt/β‑catenin signaling to restore tight junction protein expressions and localization, thereby improving intestinal barrier function, suggesting the value of EC as a novel candidate for treatment of inflammatory bowel disease.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2196-2205"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13625003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of <i>Huayu Tongluo</i> moxibustion combined with neural stem cell transplantation on hippocampal Drp1/Mfn proteins and mitochondrial dynamics in rats with vascular dementia].","authors":"Weishuai Yin, Fei Li, Yi Cao, Qiqi Yang, Zetian Deng, Shijie Cao, Yongqi Sun, Xinyang Bi, Zhengpeng Liang, Sijia Wu, Baoguo Wang","doi":"10.12122/j.issn.1673-4254.2026.07.06","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.06","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the mechanism by which <i>Huayu Tongluo</i> moxibustion (HYTLM) regulates mitochondrial dynamics of neural stem cells (NSCs) for treatment of vascular dementia (VD).</p><p><strong>Methods: </strong>Seventy-two SD rats were randomized equally into 6 groups, including a sham-operated group, a VD model group, and 4 VD groups receiving HYTLM treatment, HYTLM treatment with stereotactic injection of NSC suspension into the hippocampal CA1 region, or injections of NSC suspension or cell culture medium without HYTLM treatment. Morris water maze test was used to assess learning and memory ability of the rats. Hippocampal neuronal apoptosis, NSC differentiation, neuronal survival, histopathological changes, mitochondrial reactive oxygen species (ROS) levels, and hippocampal expressions of Drp1, Mfn1, Mfn2, and Fis1 proteins were evaluated using TUNEL assay, immunofluorescence staining, HE staining, flow cytometry, and Western blotting.</p><p><strong>Results: </strong>The rats receiving culture medium injection, similar to the VD rats, exhibited prolonged escape latency and disorganized swimming trajectories in Morris water maze test and had significantly increased neuronal apoptosis, suppressed NSC proliferation, reduced mature neurons, impaired neurogenesis, and increased ROS levels in the hippocampal CA1 region, showing also obvious neuronal injuries, lowered hippocampal Drp1, Mfn1, and Mfn2 expressions and increased Fis1 expression. All these changes were significantly alleviated in VD rats receiving NSC transplantation. HYTLM treatment produced similar effects to NSC transplantation, but their combined treatment further shortened the escape latency of the VD rats, which showed similar swimming patterns to the sham-operated rats, and produced stronger protective effects on the hippocampal neurons. The combined treatment also further reduced ROS levels and improved aberrant expressions of Drp1, Mfn1, Mfn2, and Fis1.</p><p><strong>Conclusions: </strong>HYTLM treatment improves cognitive functions of VD rats, promotes NSC differentiation into functional neurons and neuronal survival, and attenuates neuronal injury possibly by regulating hippocampal mitochondrial dynamics, thereby restoring mitochondrial homeostasis and ameliorating functional impairment.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1520-1532"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Isolation and identification of <i>Pediococcus acidilactici</i> BPA03 derived from human milk and evaluation of its antioxidant and anti-aging probiotic properties].","authors":"Longbin Cao, Peixiang Huang, Zikang Zhang, Weimin Sun, Feng Qiu","doi":"10.12122/j.issn.1673-4254.2026.07.14","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.14","url":null,"abstract":"<p><strong>Objectives: </strong>To identify <i>Pediococcus acidilactici</i> BPA03 strain isolated from healthy women's milk and evaluate its safety and probiotic properties.</p><p><strong>Methods: </strong>BPA03 strain was isolated from breast milk using dilution plating and streaking on MRS agar. Identification was performed using Gram staining, biochemical assays (BD card), 16S rRNA sequencing, and phylogenetic analysis. Safety assessment of the strain was conducted by evaluation of its hemolytic activity on blood agar, antibiotic susceptibility (Kirby-Bauer), and acute toxicity in zebrafish embryos. The probiotic properties of the bacterium were evaluated by measuring lactic acid production and assessing its protective effects against UV-induced photoaging and D-galactose-induced aging in zebrafish models.</p><p><strong>Results: </strong>The BPA03 strain was Gram-positive with a spherical or ovoid shape, and identified as <i>Pediococcus acidilactici</i>. This strain showed no hemolytic activity and was sensitive to all the tested antibiotics (penicillin, ampicillin, and vancomycin). Growth curve analysis showed a lag phase of 2 h, a logarithmic phase starting from 4 to 12 h, and a stationary phase after 20 h in aerobic culture. Lactic acid concentration reached 23.6 mmol/L after culture for 8 h in MRS medium. In zebrafish embryos, BPA03 exhibited no significant acute toxicity, and the embryos showed a hatching rate above 95% even in the presence of 10⁸ CFU/mL BPA03 (LD₅₀>10⁸ CFU/mL). In UV-exposed zebrafish models, BPA03 obviously alleviated UV-induced caudal fin damage, significantly reduced reactive oxygen species and malondialdehyde levels, enhanced antioxidant enzyme activities (superoxide dismutase, catalase and glutathione peroxidase), and downregulated mRNA expression of pro-inflammatory cytokines (IL‑1β, IL‑6, IL‑8, and TNF‑α).</p><p><strong>Conclusions: </strong><i>Pediococcus acidilactici</i> BPA03 strain isolated from breast milk of healthy women exhibits good probiotic properties and safety.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1601-1610"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[MBD1 knockdown inhibits proliferation, migration and angiogenesis of human umbilical vein endothelial cells <i>in vitro</i>].","authors":"Yutong Dan, Tingfu Zhang, Hongmei Liu, Peng Zhang","doi":"10.12122/j.issn.1673-4254.2026.07.12","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.12","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of methyl-CpG binding domain protein 1 (MBD1) on proliferation, migration, and angiogenesis in human umbilical vein endothelial cells (HUVECs).</p><p><strong>Methods: </strong>Cultured HUVECs were infected with a lentivirus carrying sh-MBD1 or a scramble shRNA (sh-NC), and the successfully infected cells were screened using puromycin. RT-qPCR and Western blotting were used to assess MBD1 gene silencing efficiency. The changes in proliferation, cell cycle, and migration of the infected cells were assessed using CCK-8 and colony formation assays, flow cytometry, scratch assay, and Transwell assay. The impact of MBD1 knockdown on angiogenic capacity of HUVECs was evaluated using angiogenesis assay. Immunofluorescence staining was used to investigate the expression of vascular endothelial growth factor A (VEGFA) in the infected HUVECs.</p><p><strong>Results: </strong>Compared with the sh-NC cells, HUVECs infected with the lentivirus carrying sh-MBD1 exhibited significantly reduced proliferation capacity and migration ability. The total length of vessels formed <i>in vitro</i>, closed vascular loops, and the number of branching points were all lower in sh-MBD1 cells than in sh-NC cells. VEGFA expression was significantly reduced in sh-MBD1 cells compared to the sh-NC cells.</p><p><strong>Conclusions: </strong>MBD1 promotes proliferation, migration, and angiogenesis of HUVECs possibly by regulating the expression of VEGFA.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1585-1592"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal association between oral microbiota and hepatocellular carcinoma in East Asian populations: a Mendelian randomization study.","authors":"Hanxiang Zhong, Yuanyu Zhao, Hong Fu, Jiayong Dong, Guoshan Ding, Yao Chen, Wenyuan Guo","doi":"10.12122/j.issn.1673-4254.2026.07.01","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.01","url":null,"abstract":"<p><strong>Objectives: </strong>Emerging evidence highlights the crucial role of the oral microbiota in various malignancies, but its causal relationship with hepatocellular carcinoma (HCC) remains largely unexplored. This study aimed to investigate the causal association between oral microbiota composition and HCC risk in East Asian populations using Mendelian randomization (MR) analysis.</p><p><strong>Methods: </strong>We performed a two-sample Mendelian randomization to investigate the causal association of 309 tongue dorsum microbiomes and 285 salivary microbiomes with liver cancer progression using the latest pooled data from genome-wide association study (GWAS) of oral microbiomes in East Asian populations. We selected single nucleotide polymorphism (SNP) independent of confounders as the instrumental variable (IV) for causal inference analysis using various Mendelian randomization statistical techniques. The heterogeneity and pleiotropy of the IV was evaluated to ensure the reliability of the results.</p><p><strong>Results: </strong>Our analysis revealed a complex association between specific bacterial genera in the oral microbiome and liver cancer. <i>Streptococcus</i> showed a mixed association with hepatocellular carcinoma, while <i>Oribacterium</i> and <i>Centipeda</i> showed a positive correlation with HCC occurrence. <i>Gemella</i> genus was negatively correlated with HCC. Heterogeneity or pleiotropy of the IV was not detected in the sensitivity analysis.</p><p><strong>Conclusions: </strong>This study provides the first Mendelian randomization evidence linking oral microbiota to HCC susceptibility in East Asian populations. Our findings suggest causal roles of specific oral bacterial taxa in hepatocarcinogenesis, and offer new insights into the mechanisms of the oral-liver axis and potential microbial targets for HCC prevention and treatment.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1467-1473"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Methyl syringate alleviates DSS-induced colitis in mice by suppressing intestinal epithelial cell apoptosis <i>via</i> inhibiting the MAPK signaling pathway].","authors":"Longtao Zhang, Yu Zhang, Tong Qiao, Keni Zhang, Lin Yin, Ju Huang, Jing Li, Zhijun Geng, Jianguo Hu","doi":"10.12122/j.issn.1673-4254.2026.07.21","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.21","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the protective effect of methyl syringate (MS) against dextran sodium sulfate (DSS)‑induced colitis in mice and the underlying mechanism.</p><p><strong>Methods: </strong>Twenty-four C57BL/6 mice were random and equally into control group, DSS model group, and MS (100 mg/kg) treatment group. The therapeutic effect of MS on colitis was assessed by measuring changes in body weight, disease activity index (DAI) score and colon length and histopathological examinations with HE and AB-PAS staining. ELISA and RT-qPCR were used to detect the expressions of IL-6, TNF-α, and IL-10 in the colon tissue, and immunohistochemistry, immunofluorescence staining, Western blotting and TUNEL staining were used to detect the expressions of myeloperoxidase (MPO), tight junction proteins ZO-1 and claudin-1, and MAPK pathway proteins as well as cell apoptosis in the colon. In cultured NCM460 cells treated with 1% DSS, the effects of MS (50 μmol/L) treatment on cell apoptosis and expressions of poptosis-related proteins and MAPK pathway proteins were evaluated using flow cytometry and Western blotting.</p><p><strong>Results: </strong>MS treatment significantly ameliorated DSS-induced body weight loss, colon shortening, increased DAI score and histological inflammation score, and intestinal pathologies in mice. MS downregulated IL-6, TNF-α, and MPO, upregulated IL-10, and restored the expression and distribution of ZO-1 and claudin-1 in the colon tissue of the mice. In the mouse and cell models, MS treatment significantly reduced apoptosis rate of intestinal epithelial cells, upregulated Bcl-2 and XIAP, and downregulated cleaved caspase-3 expressions. KEGG enrichment analysis suggested a possible association of MAPK pathway with the therapeutic effect of MS, which was confirmed by lowered phosphorylation levels of p-JNK, p-ERK, and p-p38 in both the MS-treated mouse and cell models.</p><p><strong>Conclusions: </strong>MS alleviates DSS-induced colitis in mice by reducing intestinal epithelial cell apoptosis and improving intestinal barrier damage possibly by inhibiting the MAPK signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1685-1695"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}