南方医科大学学报杂志最新文献

{"title":"[High expression of CFL1 and phospho-CFL1 predicts poor prognosis and promotes migration of colon cancer cells <i>in vitro</i>].","authors":"Linyu Wang, Xiaoyan Wang, Jijun Zhu, Bo Jiang, Jie Xu, Caiyue Gao, Dongdong Li, Yu Wang, Xiaomin Li","doi":"10.12122/j.issn.1673-4254.2026.07.07","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.07","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the expressions of cofilin-1 (CFL1) and its phosphorylated form p-CFL1 in colon cancer, their relationship with clinicopathological features and patient prognosis, and their regulatory roles in colon cancer cell migration.</p><p><strong>Methods: </strong>The expression of CFL1 and p-CFL1 and their correlation with clinicopathological parameters were analyzed using the TCGA database and immunohistochemistry of colon cancer tissue microarrays. Kaplan-Meier survival analysis was used to explore the association of CFL1/p-CFL1 expression levels with survival of colon cancer patients. The localization of CFL1 and p-CFL1 in the nucleus and cytoplasm was observed using immunofluorescence staining. Wild-type (CFL1-WT) and nuclear localization signal mutant-type (CFL1-MUT) overexpression vectors were constructed and transfected into HCT116 cells, and the changes in cell migration were assessed using Transwell chamber migration assay. Transcriptome sequencing combined with KEGG enrichment analysis was performed to identify the differentially expressed genes and signaling pathways.</p><p><strong>Results: </strong>Immunohistochemistry results showed significantly increased expressions of CFL1 and p-CFL1 in colon cancer tissues compared with the adjacent tissues (<i>P</i><0.0001), and their expression levels were positively correlated (r=0.4753, <i>P</i><0.0001). High expressions of CFL1 and p-CFL1 were significantly correlated with lymph node metastasis, advanced clinical stage, and shorter overall survival of the patients (<i>P</i><0.05). Immunohistochemistry and immunofluorescence staining demonstrated localization of CFL1 and p-CFL1 in both the cytoplasm and nucleus. Functional experiments showed that overexpression of CFL1-WT and CFL1-MUT both promoted HCT116 cell migration, and CFL1-WT exhibited a stronger effect. KEGG analysis suggested that the genes regulated by CFL1-WT were significantly enriched in tumor metastasis-related pathways involving Ras, PI3K-Akt, and cell adhesion molecules.</p><p><strong>Conclusions: </strong>CFL1/p-CFL1 are highly expressed in colon cancer and can be potential biomarkers for poor prognosis. CFL1 overexpression further activates tumor metastasis-related signaling pathways through nuclear localization, thereby promoting colon cancer migration.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1533-1542"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Remimazolam stabilizes hemodynamics during anesthesia induction but fails to reduce renal injury risk following laparoscopic partial nephrectomy: a propensity score-matched retrospective cohort study].","authors":"Changhong Sun, Fei Liu, Jiaxin Li, Han Gao, Yi Liu, Longhe Xu, Yongzhe Liu, Minglong Gao","doi":"10.12122/j.issn.1673-4254.2026.09.07","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.07","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of remimazolam administered during anesthesia induction on hemodynamics and postoperative renal function in patients undergoing laparoscopic partial nephrectomy (LPN).</p><p><strong>Methods: </strong>Clinical data of 265 renal tumor patients undergoing LPN between January, 2022 and December, 2024 were collected. The patients were divided into control group and remimazolam group (0.2 mg/kg for induction), and after propensity score matching (PSM), 65 patients in each group were included. Mean arterial pressure (MAP) and heart rate (HR) were recorded before induction (T₀), immediately after induction (T₁), immediately after intubation (T₂), and 10 min after intubation (T₃). Renal function indicators at 24 h postoperatively and incidences of acute kidney injury (AKI) were recorded. Estimated glomerular filtration rates (eGFRsCr, eGFRCys-C, and eGFRdiff) were calculated using serum creatinine (sCr) and cystatin C (Cys-C). From 36 patients in control group and 41 patients in remimazolam group followed up for 3-12 months, eGFRsCr data were collected to evaluate the incidence of chronic kidney disease (CKD) and analyze its correlation with AKI.</p><p><strong>Results: </strong>After PSM and covariate adjustment, remimazolam group showed significantly smaller changes than control group in ΔMAP at T3 (-20.11±11.65 <i>vs</i> -13.54±12.75 mmHg, <i>P</i><0.05), ΔHR at T2 (-3.78±11.18 <i>vs</i> 2.82±10.79 min^-1, <i>P</i><0.05), and ΔHR at T3 (-10.85±15.54 <i>vs</i> -3.35±11.67 min^-1, <i>P</i><0.05). The remimazolam group showed significantly higher postoperative Cys-C level than the control group [0.96 (0.96, 1.03) <i>vs</i> 0.91 (0.77, 1.01), <i>P</i><0.05] but lower eGFRCys-C at 24 h (83.65±9.84 <i>vs</i> 94.83±20.92, <i>P</i><0.05). eGFRdiff showed a significant negative shift in remimazolam group (18.55±17.06 <i>vs</i> 8.52±15.85, <i>P</i><0.05). Other indicators did not differ significantly between the two groups. AKI showed a significant correlation with CKD following LPN (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>Compared with propofol, remimazolam induction improves hemodynamic stability during anesthesia induction but does not reduce the incidences of postoperative AKI or CKD after LPN.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2069-2077"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of artificial intelligence for surgical skill assessment and quality control in minimally invasive surgery: progress, problems and prospect].","authors":"Boer Su, Ying Zhang, Zhixian Yan, Ying Zou, Yufan Cheng, Tianshu Li, Jinming Li, Xiaotong Chen, Chunqi Liang, Rong Xu, Shengming Yang, Bo Peng, Hao Chen, Jiang Yu, Yanfeng Hu","doi":"10.12122/j.issn.1673-4254.2026.07.25","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.25","url":null,"abstract":"<p><p>Minimally invasive surgery has become an important approach for treating complex surgical conditions. As such surgical procedures are often associated with high risks and technical complexities, the surgeon's skills, efficiency of team collaboration, and capacities for emergency response all significantly affect the surgical outcomes. Establishing a robust surgical skill assessment and quality control system is therefore of vital importance to reduce the risks and enhance perioperative safety. In this context, the conventional methods for surgical skill assessment and quality control (e.g., subjective scoring, postoperative analysis, etc.) appear insufficient due to their inconsistent standards, time and labor intensity, and lack of real-time feedback. In recent years, artificial intelligence (AI), particularly computer vision-based deep learning technology, has demonstrated immense potential in surgical motion recognition, workflow phase analysis, and intraoperative safety monitoring, and has propelled the development of surgical skill assessment and quality control toward automation, objectivity, and precision. Herein the authors review the latest research progress in the application of AI in minimally invasive surgical skill assessment and quality control, focusing on AI-based skill assessment and quality control algorithm models. This review also addresses model generalization, privacy protection, multicenter applicability, and future clinical applications of intelligent precision surgery.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1723-1730"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Butyrate alleviates <i>Clostridium difficile</i> infection in high-fat diet-induced obese mice].","authors":"Minyu Dong, Zeyang Fang, Yunyun Zhang, Youliang Wu, Huangjie Zheng, Yongjun Liu, Qiong Yang, Deqian Kong, Kequan Chen, Siqi Wang","doi":"10.12122/j.issn.1673-4254.2026.07.02","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.02","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the mechanism by which obesity exacerbates <i>Clostridium difficile</i> infection (CDI) and explore the value of gut microbiota metabolite butyrate as an alternative therapy.</p><p><strong>Methods: </strong>In mouse models of high-fat diet (HFD)-induced obesity and CDI and normal diet-fed mouse models of CDI, the effect of butyrate treatment on hepatic lipid deposition and liver function was assessed using Oil Red O staining and by measuring aspartate aminotransferase and alanine aminotransferase levels. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) of the mice were determined, and colonic injury was evaluated using HE staining. The expression levels of intestinal mucosal barrier proteins ZO-1 and occludin were detected by RT-PCR and immunohistochemistry, and the levels of <i>C. difficile</i> toxins A and B in mouse serum and feces were measured using RT-PCR and ELISA. The concentration of short-chain fatty acids (SCFAs) in mouse feces was determined using gas chromatography-mass spectrometry. In HCT116 cells induced by palmitic acid in the presence or absence of <i>C. difficile</i>, the effect of butyrate expression levels of ZO-1 and occludin were examined by immunofluorescence staining, and cell apoptosis was analyzed with flow cytometry.</p><p><strong>Results: </strong>Compared with normal diet-fed mice, the HFD-fed mice with CDI had a significantly higher mortality rate and exhibited severer colonic damage, intestinal barrier function impairment and liver injury with significantly reduced fecal SCFAs concentrations. Butyrate replacement therapy markedly alleviated CDI symptoms, improved intestinal barrier function, and reduced the levels of liver injury markers. Fecal microbiota transplantation produced similar effects to butyrate and obviously alleviated CDI symptoms and increased fecal SCFAs concentrations in the mouse models.</p><p><strong>Conclusions: </strong>HFD significantly exacerbates the progression of CDI in mice, and butyrate shows the potential as an alternative therapy for improving obesity-associated CDI.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1474-1486"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Wuzi Yanzong</i> Pill ameliorates diminished ovarian reserve in mice by inhibiting granulosa cell apoptosis <i>via</i> the TGF-β1/PI3K/Akt/mTOR pathway].","authors":"Yunteng Liang, Qian Gao, Yueyi Li, Jingyu Huang, Kailu Liu, Yanli Hong, Huifang Zhou, Xiaowei Nie","doi":"10.12122/j.issn.1673-4254.2026.09.17","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.17","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the efficacy of <i>Wuzi Yanzong</i> Pill (WZYZP) for improving diminished ovarian reserve (DOR) in mice and explore the underlying mechanism.</p><p><strong>Methods: </strong>Female C57BL/6J mice were randomly divided into control group, Tripterygium wilfordii polyglycoside-induced DOR model group, and two WZYZP groups treated with WZYZP at daily doses of 1.82 or 3.64 g/kg for 28 days (<i>n</i>=8). Ovarian index of the mice was calculated, hormone levels were detected with ELISA, and ovarian histopathological changes were observed using HE staining. RNA sequencing was performed to identify the key signaling pathways involved in WZYZP treatment for DOR. Network pharmacology analysis was used to predict the potential targets and pathways of WZYZP. Immunohistochemistry and Western blotting were used to detect ovarian expressions of the key proteins in the TGF‑β1/PI3K/Akt/mTOR pathway. Granulosa cell (GC) apoptosis levels were assessed with TUNEL staining and Western blotting. In addition, human GCs samples were collected from DOR patients for validating the expressions of the TGF‑β1/PI3K/Akt/mTOR pathway using Western blotting.</p><p><strong>Results: </strong>In the mouse models of DOR, WZYZP treatment significantly improved the ovarian index, increased anti-Müllerian hormone (AMH) and estradiol (E₂) levels, decreased follicle stimulating hormone (FSH) level, alleviated ovarian pathologies, enhanced GC function, and reduced atretic follicles. RNA-seq revealed significant enrichment of the PI3K/Akt pathway after WZYZP treatment. Network pharmacology analysis suggested that the TGF‑β, PI3K‑Akt, and apoptosis pathways were the key pathways potentially targeted by WZYZP. WZYZP treatment significantly upregulated the expressions of TGF‑β1, PI3K, Akt, and mTOR in mouse ovarian tissues and inhibited GC apoptosis. Western blotting of human GCs further verified the down‑regulation of TGF‑β1/PI3K/Akt/mTOR pathway in DOR patients.</p><p><strong>Conclusions: </strong>WZYZP improves ovarian reserve by upregulating the TGF‑β1/PI3K/Akt/mTOR signaling pathway and inhibiting excessive apoptosis of GCs.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2185-2195"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13625002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Hypolipidemic activity of phenolic acids from <i>Inula japonica</i> Thunb.]","authors":"Yuting Chen, Yanting You, Yushu Huang, Fei He, Feng Qiu, Qingfeng DU","doi":"10.12122/j.issn.1673-4254.2026.06.17","DOIUrl":"10.12122/j.issn.1673-4254.2026.06.17","url":null,"abstract":"<p><strong>Objectives: </strong>To isolate and identify phenolic acid constituents from methanol extract of <i>Inula japonica</i> Thunb. and evaluate their lipid-lowering activity <i>in vitro</i>.</p><p><strong>Methods: </strong>The methanol extract of <i>Inula japonica</i> Thunb. was purified using silica gel, ODS, and semi-preparative HPLC. The compound structures were determined using physicochemical and spectroscopic analyses, and their hypolipidemic activities were assessed in an AML12 cell model of free fatty acids (FFA)-induced lipid deposition. The active compounds were further assessed for their effects on lipid deposition, reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) of the cell model.</p><p><strong>Results: </strong>A total of 11 phenolic acid compounds were isolated from <i>Inula japonica</i> Thunb., namely dihydrocaffeic acid (<b>1</b>), protocatechualdehyde (<b>2</b>), chlorogenic acid (<b>3</b>), cryptochlorogenic acid (<b>4</b>), 1,3-O-dicaffeoylquinic acid (<b>5</b>), 2,3-dicarboxy-6,7-hydroxy-l-(3',4'-dihydroxy)-phenyl -1,2-dihydronaphthalene-10-methyl ester (<b>6</b>), caffeic acid (<b>7</b>), protocatechuic acid (<b>8</b>), 3-carboxy-6,7-dihydroxy-1-(3',4'-dihydroxyphenyl)-naphthalene (<b>9</b>), p-hydroxybenzoic acid (<b>10</b>), and 4-hydroxyphenylacetic acid (<b>11</b>). Among them, compounds <b>1</b> and <b>9</b> were isolated for the first time from the genus <i>Inula</i>, and compound <b>6</b> was isolated for the first time from this plant. Compounds <b>6</b> and <b>9</b> showed lipid-lowering effects in FFA-induced AML12 cells, significantly lowered cellular levels of total cholesterol, triglyceride, alanine aminotransferase, and aspartate aminotransferase, reduced lipid accumulation, and alleviated oxidative stress and mitochondrial function.</p><p><strong>Conclusions: </strong><i>Inula japonica</i> Thunb. contains diverse phenolic acids, and among them compounds <b>6</b> and <b>9</b> show hypolipidemic activities via inhibiting lipid accumulation and improving oxidative stress and mitochondrial function and can potentially serve as lead compounds for treatment of metabolic dysfunction-associated steatotic liver disease.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 6","pages":"1374-1384"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148318251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism of <i>Tongxie Yaofang</i> for reducing 5-HT-induced oxidative stress injury in BRL 3A cells: the mediating role of the SREBP-1/CD1 pathway].","authors":"Minglang Song, Hai Su, Hongxing Xia, Shiyu Chen, Lili Shao, Zhibin Jiang","doi":"10.12122/j.issn.1673-4254.2026.06.018","DOIUrl":"10.12122/j.issn.1673-4254.2026.06.018","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the role of the SREBP-1/SCD1 pathway in mediating the protective effect of <i>Tongxie Yaofang</i> (TXYF) against 5-hydroxytryptamine (5-HT)-induced injury in BRL 3A cells.</p><p><strong>Methods: </strong>Fourteen SD rats were gavaged with TXYF for 7 consecutive days to prepare TXYF-medicated serum. BRL 3A cells in routine culture were examined for changes in cell viability using CCK-8 assay following treatment with different concentrations of 5-HT. In BRL 3A cells treated with 5-HT and different concentrations of the medicated serum, SOD, MDA, and ROS levels were measured, and mitochondrial membrane potential and cell morphology were evaluated using JC-1 staining and electron microscopy; autophagosomes and LC3B expression were detected with immunofluorescence staining, and the protein expression levels of Cyt-c, caspase-3, Gsk3β, Beclin-1, LC3 I/II, p62, SREBP-1, SCD1, and 5-HT_2AR were analyzed using Western blotting.</p><p><strong>Results: </strong>The optimal 5-HT concentration for modeling was 1.5 mmol/L. In 5-HT-treated cells, treatment with TXYF-medicated serum significantly reduced cellular ROS and MDA levels, increased SOD activities, lowered mitochondrial membrane potential, and alleviated mitochondrial swelling. The cells treated with TXYF-medicated serum also showed decreased expression levels of LC3B, MDC, Beclin-1, LC3 II/LC3 I, Cyt-c, SCD1 and 5-HT_2AR and increased expressions of p62 and SREBP-1 without significant changes in caspase-3 and Gsk3β expressions.</p><p><strong>Conclusions: </strong>TXYF-medicated serum alleviate oxidative stress and autophagy dysfunction via the SREBP-1/SCD1 pathway to protect BRL 3A cells against 5-HT-induced injury.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 6","pages":"1385-1394"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148318284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficacy of CD4/TGF-β bispecific antibody in a mouse model of peritoneal metastasis of malignant melanoma].","authors":"Qianli Jiang, Taosong Liu, Zizhan Wang, Shan Jiang, Haiyang Wu, Yongzhen Lin, L U Shiying, Lingxi Chen, Jiaxing Zhang, Luxia Tang, Rakesh Kumar Raut, Zhongyuan Xu","doi":"10.12122/j.issn.1673-4254.2026.06.013","DOIUrl":"10.12122/j.issn.1673-4254.2026.06.013","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the efficacy of CD4/TGF-β bispecific antibody in a mouse model of peritoneal metastasis of melanoma.</p><p><strong>Methods: </strong>For drug safety testing, 20 human CD4 transgenic C57BL/6J mice were randomized into 4 groups (<i>n</i>=5) for intravenous injections of PBS or 2.5, 5, or 10 mg/kg CD4/TGF‑β bispecific antibody, and the changes in general condition, body weight and body temperature were observed. Another 20 transgenic C57BL/6J mice were randomized into two groups to receive intraperitoneal injection of magnetized B16F10-GL melanoma cells expressing green fluorescent protein and luciferase with or without application of a magnet (3 mm in diameter) to the right abdominal skin before cell injection. Three days later, each group was further divided into two groups for treatment with PBS or CD4/TGF-β bispecific antibody (300 μg) twice a week. <i>In vivo</i> imaging was performed at different time points to assess fluorescence distribution and intensity. On day 14, the mice were euthanized and tumor burden and dissemination were evaluated by gross observation, histopathological analysis, immunofluorescence staining, and RT-qPCR.</p><p><strong>Results: </strong>Injection of the antibody did not produce any significant adverse effects in the mice. In the tumor-bearing mice, the application of a magnet significantly accelerated tumor development (3.80±1.79 <i>vs</i> 9.20±2.17 days; <i>P</i>=0.003) and resulted in precise and consistent tumor formation in the parietal peritoneum. Magnet application did not significantly affect survival of the mice but significantly prolonged the therapeutic window (4.60±1.95 <i>vs</i> 10.00±1.73 days; <i>P</i>=0.002). The mice treated with CD4/TGF‑β bispecific antibody had significantly reduced tumor burden irrespective of the inoculation approach, and showed dense encapsulation of the tumor foci by type III collagen, whereas minimal type III collagen deposition and abundant tumor cells were observed in PBS-treated mice. Treatment with the antibody significantly downregulated the expression of melanoma-associated gene MITF, and the reduction was more pronounced in the magnet group.</p><p><strong>Conclusions: </strong>The CD4/TGF‑β bispecific antibody shows significant antitumor efficacy and good safety in the mouse model of peritoneal metastasis of melanoma.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 6","pages":"1331-1338"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148318188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A bidirectional feature mapping classification model based on multi-constrained latent representation learning for differential diagnosis of pneumonia].","authors":"Min Zeng, Li Zhuo, Shunqian Tan, Xin Zhen","doi":"10.12122/j.issn.1673-4254.2026.06.23","DOIUrl":"10.12122/j.issn.1673-4254.2026.06.23","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a bidirectional feature-mapping classification model for differential diagnosis of pneumonia.</p><p><strong>Methods: </strong>We collected chest X-ray (CXR) images from 1457 patients with pneumonia and 1456 healthy individuals. Radiomic features extracted from the segmentation masks using PyRadiomics were mapped into a latent shared space to construct the classification model using the bidirectional feature mapping classification model based on multi-constrained latent representation learning. The performance of the constructed model for differential diagnosis of pneumonia was evaluated using 5-fold cross-validation and compared with other feature-based classification models. Decision curve analysis was used for evaluating clinical utility of the model, and ablation experiments were performed to assess the contribution of each constraint module. The importance of the radiomics features was interpreted using the SHAP method, and a two-dimensional visualization experiment of the low-dimensional latent features obtained through the proposed mapping method was conducted to verify the feasibility and effectiveness of the model.</p><p><strong>Results: </strong>The 5-fold cross-validation results showed that the proposed classification model had a positive predictive value of 0.796, a negative predictive value of 0.830, a specificity of 0.784, a sensitivity of 0.830, an accuracy of 0.811, and an area under the ROC curve of 0.893 for differential diagnosis of pneumonia. Decision curve analysis demonstrated a high net clinical benefit of the model within acceptable threshold probabilities. Ablation studies confirmed the essential role of the multi-constraint module, and the SHAP analysis revealed that the model focused primarily on clinically meaningful and medically interpretable features. The feature mapping method exhibited excellent performance in visual experiments to confirm the effectiveness of the proposed model.</p><p><strong>Conclusions: </strong>The proposed bidirectional feature mapping classification model demonstrates strong discriminative capability and high potential for differential diagnosis of pneumonia and shows obvious advantages over other classification models in pneumonia classification tasks.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 6","pages":"1434-1443"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148318246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Overexpressing NDUFA13 and CRIP1 improves B cell injury and lung Lesions in COPD mouse models].","authors":"Miao Wang, Bichao Lu, Haoyang Meng, Shuhan Zhou, Ningfeng Mao, Yang Zhang, Wenliang Lü","doi":"10.12122/j.issn.1673-4254.2026.06.15","DOIUrl":"10.12122/j.issn.1673-4254.2026.06.15","url":null,"abstract":"<p><strong>Objectives: </strong>To identify B cell-associated biomarkers in chronic obstructive pulmonary disease (COPD) using bioinformatics and explore their diagnostic and therapeutic potential.</p><p><strong>Methods: </strong>Based on the GEO database, differential expression analysis, WGCNA, scRNA-seq, ROC curve analysis, and Mendelian randomization analysis were performed to screen B cell-related biomarkers. Twenty ICR mice were divided equally into control and COPD groups, and COPD model was established using lipopolysaccharide (LPS) combined with cigarette smoke exposure to verify biomarker expression. Cultured murine B lymphoma CH12.LX cells were stimulated with 1 μg/mL LPS and transfected with plasmids overexpressing CRIP1 or NDUFA13 or with a negative control plasmid, and the changes in protein expressions of NDUFB8, MTCO1, ATP5A and ACTB were examined with Western blotting. CCK-8 assay and flow cytometry were used to analyze the viability and apoptosis of murine lung epithelial cells co-cultured with the treated CH12.LX cells, respectively.</p><p><strong>Results: </strong>Two protective factors, namely NDUFA13 (OR=0.2598) and CRIP1 (OR=0.2690; <i>P</i><0.0001), were identified as the key B cell-associated biomarkers in COPD. In the mouse models of COPD, the expressions of NDUFA13 and CRIP1 were significantly downregulated in the lung tissues at both the transcriptional and protein levels. In CH12.LX cells, stimulation with LPS significantly lowered the protein expressions of NDUFA13, CRIP1, NDUFB8, MTCO1 and ATP5A. Overexpression of CRIP1 or NDUFA13 effectively inhibited LPS-induced inflammatory responses and oxidative stress in CH12.LX cells, and promoted the viability and suppressed apoptosis of lung epithelial cells co-cultured with CH12.LX cells.</p><p><strong>Conclusions: </strong>NDUFA13 and CRIP1 are B cell-associated protective biomarkers in COPD with potential diagnostic values for COPD.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 6","pages":"1349-1364"},"PeriodicalIF":0.0,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148318034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}