南方医科大学学报杂志最新文献

{"title":"[Role of Abelson interactor 2 in progression and prognosis of gastric cancer and its regulatory mechanisms].","authors":"X Chen, H Lu, Z Wang, L Wang, Y Xia, Z Geng, X Zhang, X Song, Y Wang, J Li, J Hu, L Zuo","doi":"10.12122/j.issn.1673-4254.2024.09.04","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.04","url":null,"abstract":"<p><strong>Objective: </strong>To explore the regulatory role of Abelson interactor 2 (ABI2) in progression and prognosis of gastric cancer.</p><p><strong>Methods: </strong>TIMER2.0, GEPIA, Kaplan-Meier Plotter and DAVID databases were used to analyze ABI2 expression in pancancer and its association with the prognosis of gastric cancer. Gastric cancer and adjacent tissues from 120 patients undergoing radical gastrectomy in our hospital between January, 2016 and October, 2018 were examined for ABI2 expression and its correlation with disease progression and prognosis. MGC-803 cell models of ABI2 knockdown and overexpression were established for observing the changes in cell proliferation, migration, and invasion, and the impact of ABI2 expression modulation on xenograft growth was evaluated in nude mice.</p><p><strong>Results: </strong>Database analysis and examination of the clinical samples showed that ABI2 was highly expressed in gastric cancer tissues. Survival analysis suggested that gastric cancer patients with a high expression of ABI2 had a reduced postoperative 5-year survival rate (<i>P</i> < 0.0001), and further Cox univariate and multivariate survival analyses indicated that a high ABI2 expression was an independent risk factor affecting the patients survival outcomes (<i>P</i>=0.022, <i>HR</i>=1.887, 95% <i>CI</i>: 1.096-3.249). Enrichment analysis suggested the involvement of ABI2 in Wnt signaling. In MGC-803 cells, ABI2 overexpression promoted cell proliferation and xenograft growth in nude mice, increased the expressions of vimentin and N-cadherin, and lowered E-cadherin expression, while ABI2 knockdown produced the opposite effects. Mechanistic analysis revealed that ABI2 overexpression promoted the expressions of Wnt2 and β-catenin in both MGC-803 cells and the xenografts, and their expressions were significantly lowered by ABI2 knockdown.</p><p><strong>Conclusion: </strong>ABI2 is highly expressed in gastric cancer, which affects long-term prognosis of the patients, possible due to its regulatory effect on Wnt signaling to promote proliferation, migration and invasion of gastric cancer cells.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1653-1661"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A deep learning model based on magnetic resonance imaging and clinical feature fusion for predicting preoperative cytokeratin 19 status in hepatocellular carcinoma].","authors":"W Fang, H Xiao, S Wang, X Lin, C Chen","doi":"10.12122/j.issn.1673-4254.2024.09.14","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.14","url":null,"abstract":"<p><strong>Objective: </strong>To establish a deep learning model for testing the feasibility of combining magnetic resonance imaging (MRI) deep learning features with clinical features for preoperative prediction of cytokeratin 19 (CK19) status of hepatocellular carcinoma (HCC).</p><p><strong>Methods: </strong>A retrospective experiment was conducted based on the data of 116 HCC patients with confirmed CK19 status. A single sequence multi-scale feature fusion deep learning model (MSFF-IResnet) and a multi-scale and multimodality feature fusion model (MMFF-IResnet) were established based on the hepatobiliary phase (HBP), diffusion weighted imaging (DWI) sequences of enhanced MRI images, and the clinical features significantly correlated with CK19 status. The classification performance of the models were evaluated to assess the effectiveness of the deep learning models for predicting CK19 status of HCC before surgery.</p><p><strong>Results: </strong>Multivariate analysis showed that an increased neutrophil-to-lymphocyte ratio (<i>P</i>=0.029) and incomplete tumor capsule (<i>P</i>=0.028) were independent predictors of CK19 expression in HCC. The deep learning models improved by multi-scale feature fusion and multi-modality feature fusion methods achieved better classification results than the traditional machine learning models and baseline models, and the final MMFF-IResnet model showed the best classification performance with an AUC of 84.2%, an accuracy of 80.6%, a sensitivity of 80.1% and a specificity of 81.2%.</p><p><strong>Conclusion: </strong>The multi-scale and multi-modality feature fusion model based on MRI and clinical parameters is capable of predicting CK19 status of HCC, demonstrating the feasibility of combining deep learning methods with MRI and clinical features for preoperative prediction of CK19 status.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1738-1751"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Reoxygenation improves reduced hypothalamic leptin responsiveness induced by intermittent hypoxia in obese rats].","authors":"M Dong, T Zhu, J Ma, X DU, Y Feng","doi":"10.12122/j.issn.1673-4254.2024.09.09","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.09","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of intermittent hypoxia-reoxygenation (IHR) on body weight, diet and water intake, circulating metabolites, and responses to central leptin injection in a rat model of diet-induced obesity (DIO).</p><p><strong>Methods: </strong>Rat models of DIO established by 12-week high-fat diet (HFD) feeding were randomized into normoxia group (<i>n</i>=15), intermittent hypoxia group (6% O₂, 30 cycles/h, 8 h/day for 4 weeks; <i>n</i>=15), and IHR group (2 weeks of intermittent hypoxia followed by 2 weeks of reoxygenation; <i>n</i>=15). Body weight, diet and water intake of the rats were recorded, and circulating leptin, IL-6, and Ang-II levels were detected. After IHR treatment, the rats received intracerebroventricular injection of 4 μg leptin, and the hypothalamus and liver were taken 1 h later for detecting POMC, FRA-1 and FRA-2 expressions in the hypothalamus using immunohistochemistry, POMC, pSTAT3 and LepR expressions in the hypothalamus using Western blotting, and LepR mRNA expression in the hypothalamus and liver using RT-PCR.</p><p><strong>Results: </strong>The rats in intermittent hypoxia group showed significantly increased weight gain, food intake and elevated systemic inflammatory cytokine levels. Intermittent hypoxia obviously inhibited the expression of POMC, lowered the expressions of FRA-1 and pSTAT3, reduced the responsiveness of the rats to exogenous leptin, and downregulated the mRNA and protein expression of LepR. Two weeks of reoxygenation treatment obviously reduced intermittent hypoxia-induced weight gain and metabolic disorder and improved leptin sensitivity of the rats.</p><p><strong>Conclusion: </strong>Prolonged intermittent hypoxia impairs hypothalamic leptin signaling by downregulating LepR expression to promote weight gain in obese rats, which can be improved by reoxygenation treatment.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1696-1703"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of chronic intermittent hypoxia and reoxygenation on insulin resistance and skeletal muscle miR-27a-3p/PPARγ/IRS1/PI3K/AKT expressions in rats].","authors":"X Zhou, H Li, Q Chen, M Jin, H Li, W Bai, C Jia, C Wei","doi":"10.12122/j.issn.1673-4254.2024.09.13","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.13","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of chronic intermittent hypoxia (CIH) and reoxygenation on insulin resistance (IR) and expressions of miR-27a-3p/PPARγ/IRS1/PI3K/AKT in rat skeletal muscle.</p><p><strong>Methods: </strong>GEO database was used for screening the differentially expressed miRNAs in CIH, and their target genes were subjected to GO and KEGG enrichment analysis followed by construction of the miRNA-mRNA-pathway regulatory network using Cytoscape. In the animal experiment, 48 male SD rats were randomly divided into normoxia group and CIH group (8 weeks of CIH followed by 4 weeks of normoxic recovery). Blood and skeletal muscle samples were collected at baseline, 8 weeks, and 12 weeks to evaluate the changes in fasting blood glucose (FBG) and fasting insulin (FINS) levels and muscular pathology. RT-qPCR and Western blotting were used to detect the changes in the expressions of miR-27a-3p, PPARγ, GLUT4, IRS1, p-IRS1, PI3K, p-AKT and AKT in the muscular tissues.</p><p><strong>Results: </strong>No muscular miRNA datasets for CIH were available in GEO database, from which only a kidney-related dataset (GSE202480) was obtained, based on which a total of 165 differentially expressed miRNAs were identified. GO/KEGG analysis suggested that these miRNAs were involved in muscular regulation and insulin signaling. The miRNA-mRNA-pathway network highlighted miR-27a-3p as a crucial regulator in the PPAR and PI3K/AKT pathway. In the animal experiment, the rats subjected to CIH for 8 weeks showed significantly increased FBG, FINS, HOMA-IR, and PPARγ levels, loose muscle fiber arrangement, decreased cross-sectional area of the muscle fibers, and lowered expressions of miR-27a-3p, p-IRS1/IRS1, PI3K, and p-AKT/AKT in the skeletal muscles.</p><p><strong>Conclusion: </strong>CIH increases IR, causes skeletal muscle pathology, downregulates miR-27a-3p expression, upregulates PPARγ expression, and inhibits IRS1/PI3K/AKT insulin signaling in the skeletal muscles of rats, and these changes can be reversed by reoxygenation. MiR-27a-3p may participate in CIH-induced IR by modulating the PPAR γ/IRS1/PI3K/AKT signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1729-1737"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[RgpB contributes to chemoresistance in esophageal squamous cell carcinoma by preventing Cx43 degradation <i>via</i> inhibiting autophagosome-lysosome fusion].","authors":"Y DU, X Zhang, K Zhou, X Jin, X Yuan, S Gao","doi":"10.12122/j.issn.1673-4254.2024.09.06","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.06","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism through which RgpB, a virulence factor of <i>Porphyromonas gingivalis</i> (Pg), induces chemoresistance in esophageal squamous carcinoma.</p><p><strong>Methods: </strong>The autophagy-regulating factors that interact with RgpB were screened by immunoprecipitation-mass spectrometry. The interaction between RgpB and the autophagy regulator TBC1D5 was investigated using co-immunoprecipitation. The impact of Pg infection on the expression of esophageal cancer cell membrane receptor molecule Cx43 was assessed using Western blotting. Immunofluorescence assay was used to analyze the relationship among Lamp1, Cx43 and TBC1D5. The effect of Pg infection on autophagosome-lysosome fusion was evaluated using autophagy double fluorescence technique. The effects of Pg infection and a Cx43 inhibitor on proliferation of esophageal cancer cells after chemotherapy were examined with plate cloning assay and CCK-8 method.</p><p><strong>Results: </strong>Immunoprecipitation-mass spectrometry identified TBC1D5 as an autophagy regulator interacting with RgpB, and coimmunoprecipitation suggested that RgpB could directly bind to TBC1D5. In Pg-infected esophageal cancer cells, the expression of Cx43 on the cell membrane was significantly higher than that in non-infected cells. Immunofluorescence assay showed that the expression of Cx43 on the membrane of esophageal cancer cells increased significantly after Pg infection, which blocked autophagosome-lysosome fusion as shown by stubRFP-sensGFP-LC3 lentivirus study. Plate cloning assay and CCK-8 assay showed that the Cx43 inhibitor significantly attenuated the effect of Pg infection for promoting proliferation of esophageal cancer cells after chemotherapy.</p><p><strong>Conclusion: </strong>Pg infection in esophageal cancer blocked autophagosome-lysosome fusion in the tumor cells, thereby preventing Cx43 from lysosomal degradation and leading to chemoresistance of esophageal cancer.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1670-1676"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>HNRNPA1</i> gene is highly expressed in colorectal cancer: its prognostic implications and potential as a therapeutic target].","authors":"K Ji, G Yu, L Zhou, T Zhang, Q Ling, W Man, B Zhu, W Zhang","doi":"10.12122/j.issn.1673-4254.2024.09.08","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.08","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression level of HNRNP A1 in colorectal cancer (CRC) and its prognostic implications.</p><p><strong>Methods: </strong>We investigated HNRNP A1 expression level in CRC using HPA, TIMER, and GEPIA databases and analyzed its association with Ki-67 and VEGFA expressions. Kaplan-Meier Plotter database was used to analyze the correlation of HNRNP A1 mRNA levels with the survival rates of CRC patients. Pathway enrichment analysis was performed for predicting the biological roles of HNRNP A1 in CRC progression. Immunohistochemistry and Western blotting were used to examine the protein levels of HNRNP A1 in CRC versus adjacent tissues, and TIMER was used for assessing its expression in the infiltrating immune cells. In RKO/Caco2 cells, the effects of lentivirus-mediated knockdown of HNRNP A1 on cell proliferation and migration were observed, and the inhibitory effect of VPC-80051 (a HNRNP A1 inhibitor) on cell proliferation was evaluated to assess its potential as a therapeutic agent.</p><p><strong>Results: </strong>HNRNP A1 was significantly overexpressed in CRC tissues and correlated with a poor prognosis of the patients. HNRNP A1 expression level was correlated with the infiltrating immune cells in CRC microenvironment and positively correlated with MKI67 and VEGFA expressions in CRC. A high HNRNP A1 expression predicted a in survival and progression-free survival of CRC patients and was involved in multiple biological processes related with CRC progression. In RKO/Caco2 cells, HNRNP A1 knockdown significantly suppressed cell proliferation and migration, and treatment with VPC-80051 also effectively inhibited CRC cell proliferation. Immunohistochemical study demonstrated a close correlation of HNRNP A1 overexpression with tumor stage of CRC.</p><p><strong>Conclusion: </strong>HNRNP A1 is overexpressed in CRC tissues to modulate cell proliferation and migration and is correlated with a poorer prognosis. VPC-80051 can effectively inhibit CRC cell proliferation, suggesting the potential of HNRNP A1 as a therapeutic target for CRC.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1685-1695"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Lycium barbarum</i> glycopeptide reduces bone loss caused by exosomes derived from human gingival fibroblasts with radiation exposure].","authors":"S He, N Wen, X Chen, Y Wang, T Zhang, Y Mu","doi":"10.12122/j.issn.1673-4254.2024.09.15","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2024.09.15","url":null,"abstract":"<p><strong>Objective: </strong>To explore the protective effect of Lycium barbanun glycopeptide (LbGP) against osteogenic inhibition induced by exosomes derived from human gingival fibroblasts (HGFs) exposed to radiation.</p><p><strong>Methods: </strong>Cultured HGFs with or without LbGP pretreatment were exposed to 8 Gy X-ray radiation, and the changes in cell apoptosis, senescence and α-SMA level were detected using RT-qPCR, Western blotting and β-galactosidase staining. The exosomes secreted by the treated cells were extracted, and after identification by electron microscopy, particle size analysis and Western blotting, the exosomes were added into primary cultured bone mesenchymal stem cells (BMSCs), and osteoclast activity and osteogenesis in the cell cultures were detected by Trap staining, Alizarin red staining, ALP staining, RT-qPCR and Western blotting.</p><p><strong>Results: </strong>In cultured HGFs, X-ray radiation significantly increased the percentage of senescent cells, which was obviously lowered by LbGP treatment. X-ray radiation significantly reduced Bcl-2/Bax ratio and increased <i>α</i> -SMA expression in HGFs, and these changes were significantly suppressed by LbGP pretreatment. In rat BMSCs, incubation with the exosomes derived from HGFs with radiation exposure caused a significant increase of osteoclasts, reduced calcium nodules and lowered alkaline phosphatase expression in the cells; The opposite changes were observed in the cells treated with exosomes from LbGPpretreated HGFs, which also significantly increased the cellular expressions of the osteogenic genes (BMP2, ALP, and RUNX2) and proteins (ALP and RUNX2) as compared with the exosomes from irradiated HGFs.</p><p><strong>Conclusion: </strong>LbGP can effectively inhibit osteoclast activity and promote osteogenesis by acting on exosomes secreted by irradiated HGFs, suggesting its potential value for treatment of osteoradionecrosis of the jaw.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1752-1759"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[An autoencoder model based on one-dimensional neural network for epileptic EEG anomaly detection].","authors":"J Ou, C Zhan, F Yang","doi":"10.12122/j.issn.1673-4254.2024.09.20","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2024.09.20","url":null,"abstract":"<p><strong>Objective: </strong>We propose an autoencoder model based on a one-dimensional convolutional neural network (1DCNN) as the feature extraction network for efficient detection of epileptic EEG anomalies.</p><p><strong>Methods: </strong>The local information of normal EEG signals was captured by utilizing the local feature extraction ability of 1DCNN for training of an autoencoder to learn the expression of normal EEG data in low dimensional feature space. With the difference between the input and output as the anomaly score, the threshold was determined by the optimal equilibrium point of the ROC curve, and the EEG signals exceeding the threshold were diagnosed as the seizure data. The performance of the 1DCNN-AE epilepsy detection model was evaluated using the publicly available CHB-MIT scalp EEG dataset and TUH scalp EEG dataset.</p><p><strong>Results: </strong>The AUC of the 1DCNN-AE model reached 0.890 of CHB-MIT and 0.686 of TUH under the average level of patients, and the epilepsy detection rate reached 0.974 and 0.893, and these results were better than the latest epilepsy anomaly detection models LSTM-VAE and GRU-VAE. The 1DCNN model had a parameter quantity of 58.5M, which was at the same level with LSTM-VAE (47.4 M) and GRU-VAE (36.9 M) but with much smaller FLOPs (0.377 G) than LSTM-VAE (21.6 G) and GRU-VAE (16.2 G).</p><p><strong>Conclusion: </strong>The autoencoder model based one-dimensional convolutional neural network can effectively detect abnormal EEG signals in epileptic seizure.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1796-1804"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High expression of CREM is associated with poor prognosis in gastric cancer patients].","authors":"M Ye, H Wu, Y Mei, Q Zhang","doi":"10.12122/j.issn.1673-4254.2024.09.18","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.18","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the expression of CREM in gastric cancer (GC) and its correlation with prognosis of the patients.</p><p><strong>Methods: </strong>TCGA and GEO databases were used to analyze the expression levels of CREM mRNA in GC and adjacent tissues. Immunohistochemistry was used to examine the expression of CREM protein in 43 pairs of GC and adjacent tissues, and the correlation of CREM expression with clinicopathological features of the patients was analyzed. Kaplan-Meier survival analysis was used to explore the relationship between CREM expression and survival of GC patients. LinkedOmics database was used to annotate the GO function and KEGG pathway enrichment of CREM-related genes.</p><p><strong>Results: </strong>Database analysis showed that CREM was highly expressed in GC tissues (<i>P</i> < 0.05) and positively correlated with poor prognosis in GC patients (<i>P</i>=0.01). Immunohistochemistry results showed significantly higher CREM expression in GC tissues than in paired adjacent tissues (<i>P</i> < 0.0001), and its expression level was correlated with T-stage and N-stage of the tumor (<i>P</i> < 0.05). The overall survival of GC patients with high expression of CREM was shorter (<i>RR</i>=4.02, <i>P</i>=0.0046). Gene enrichment analysis showed that high CREM expression promotes occurrence and progression of GC very likely through the cell adhesion signaling pathway.</p><p><strong>Conclusion: </strong>CREM is highly expressed in GC, and its high expression is associated with a poor prognosis of GC patients, suggesting the potential of CREM to serve as a prognostic indicator for GC.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1776-1782"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficacy and safety of transcatheter arterial chemoembolization followed by hepatic arterial infusion chemotherapy combined with TKI and PD-1 inhibitors as first-line treatment for advanced hepatocellular carcinoma].","authors":"L Zhang, X Liu, X Hu, J Wang, X Yu, G Li, H You, Q Zhang, H Zhang","doi":"10.12122/j.issn.1673-4254.2024.09.24","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.24","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy and safety of transcatheter arterial chemoembolization (TACE) followed by hepatic arterial infusion chemotherapy (HAIC) combined with TKI drugs and PD-1 inhibitors as the first-line treatment for advanced hepatocellular carcinoma (HCC).</p><p><strong>Methods: </strong>We retrospectively analyzed the data of 70 patients with advanced HCC treated in the Department of Oncology of Guangdong Provincial Hospital of Integrated Traditional Chinese and Western Medicine between July, 2020 and June, 2023. 23 of the patients received TACE combined with HAIC and TKI (TACE+HAIC+ TKI group) and 47 received TACE combined with HAIC, PD-1 inhibitors and TKI (TACE+HAIC+PD-1+TKI group). The clinical characteristics, laboratory test results, efficacy, outcomes and adverse events of the patients were compared between the two groups.</p><p><strong>Results: </strong>The TACE+HAIC+TKI and TACE+HAIC+PD-1+TKI groups had significantly different objective remission rates (ORR; 60.87% <i>vs</i> 36.17%, <i>P</i>=0.031), comparable disease control rates (95.65% <i>vs</i> 93.62%, <i>P</i>=0.068), and different median progression-free survival (PFS) time (10.2 <i>vs</i> 11.8 months, <i>P</i>=0.003) and median overall survival (OS) time (15.7 <i>vs</i> 19.5 months, <i>P</i>=0.035). After propensity score matching (PSM), the median PFS and OS time of the two groups was 10.1 <i>vs</i> 14.5 months (<i>P</i>= 0.024) and 14.2 <i>vs</i> 21.2 months (<i>P</i>=0.221), respectively. The 1-year PFS rates of the 2 groups were 24.0% <i>vs</i> 52.2%, and the 1-, 2-and 3-year OS rates were 72.3% <i>vs</i> 93.1%, 23.9% <i>vs</i> 63.8%, and 23.9% <i>vs</i> 36.5%, respectively. The incidence of proteinuria was significantly higher in TACE+HAIC+PD-1+TKI group than in TACE+HAIC+TKI group (21.28% <i>vs</i> 0, <i>P</i>=0.025), but the incidences of grade 3-4 treatment-related adverse events were all similar between the two groups.</p><p><strong>Conclusion: </strong>The first-line treatment with TACE+HAIC+PD-1+TKI is safe and effective for advanced HCC and can significantly prolong the survival of the patients.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":"44 9","pages":"1831-1838"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}