Genetics and molecular research : GMR最新文献_第3页

Association between GSTM1, GSTT1, and GSTP1 polymorphisms and gastric cancer risk, and their interactions with environmental factors. GSTM1、GSTT1和GSTP1多态性与胃癌风险的关系及其与环境因素的相互作用

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16018877

Zhe Chen, J. Xian, Liangping Luo

{"title":"Association between GSTM1, GSTT1, and GSTP1 polymorphisms and gastric cancer risk, and their interactions with environmental factors.","authors":"Zhe Chen, J. Xian, Liangping Luo","doi":"10.4238/gmr16018877","DOIUrl":"https://doi.org/10.4238/gmr16018877","url":null,"abstract":"Glutathione S-transferase (GST) is an important member of phase II metabolic enzymes; GSTM1, GSTT1, and GSTP1 belong to three subfamilies of the GST enzyme. Polymorphisms in GSTM1, GSTT1, and GSTP1 could affect detoxification processes, and increase individuals' susceptibility to cancers. We aimed to investigate the association between GSTM1, GSTT1, and GSTP1 polymorphisms and the risk of gastric cancer in a Chinese population. In addition, we also examined the effect of gene-environmental interactions, and their effect on risk of this cancer. Between July 2013 and June 2015, we recruited 242 gastric cancer patients and 396 healthy controls for our study. Polymerase chain reaction-restriction fragment length polymorphism analysis was used to characterize genetic polymorphisms in GSTM1, GSTT1, and GSTP1. We observed that the Val/Val genotype of GSTP1 was associated with increased risk of gastric cancer when compared with the Ile/Ile genotype (OR = 3.19, 95%CI = 1.84-5.56). Moreover, the Val allele of GSTP1 was associated with higher susceptibility to gastric cancer as compared with the Ile allele (OR = 1.52, 95%CI = 1.19-1.93). However, GSTM1 and GSTT1 polymorphisms did not affect the development of gastric cancer. In conclusion, our study indicated that GSTP1 Ile105Val, but not GSTM1 and GSTT1 polymorphisms, was associated with risk of gastric cancer.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126232387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Early selection of sugarcane using path analysis. 利用通径分析进行甘蔗早期选育。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019038

R. P. Barbosa, F. A. Neto, L. M. Gravina, G. Gravina, M. Portela, A. Bezerra

{"title":"Early selection of sugarcane using path analysis.","authors":"R. P. Barbosa, F. A. Neto, L. M. Gravina, G. Gravina, M. Portela, A. Bezerra","doi":"10.4238/gmr16019038","DOIUrl":"https://doi.org/10.4238/gmr16019038","url":null,"abstract":"The aim of this study was to analyze sugarcane (Saccharum officinarum) biometric and technological data, obtained at different timepoints, using path analysis. The experiment was conducted in União, PI, Brazil, and evaluated 12 sugarcane genotypes (RB036066, RB9438, RB935744, RB021764, RB021754, RB021534, RB966229, RB977540, RB863129, and RB987935, and the varieties RB92579 and RB867515 as controls) in a randomized block design with four replications. Data were collected at six timepoints that were spaced 30 days apart (90, 120, 150, 180, 210, and 240 days). Direct and indirect effects of the following production components were compared: stalk length, stalk diameter, internode length, number of tillers, number of green leaves, and stalk dry matter. The technological variables evaluated were total recoverable sugar, degrees Brix, tons of polarization (pol, apparent sucrose content) per hectare, juice purity, fiber, juice pol, and tons of sugarcane per hectare. The coefficients of determination were high in all path analyses, suggesting that the components evaluated explained a large part of the variation in stalk production and in the technological variables. Stalk diameter was the trait that best correlated with stalk dry matter yield at all timepoints, with positive values that were higher than the residual effect. This demonstrates the possibility of obtaining significant gains via indirect selection for stalk dry matter yield via stalk diameter or via stalk diameter and number of tillers. The technological variables degrees brix and juice pol were the traits that best correlated with total recoverable sugar production, indicating that they could be used to indirectly select for total recoverable sugar.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125788786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development and characterization of microsatellite markers for Solen grandis using Illumina sequencing approach. 利用Illumina测序方法开发和鉴定大梭伦的微卫星标记。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019621

Y. Feng, H. Jiang, Y. Liu, X. Wu, X. Q. Liu, X. M. Wei

引用次数: 1

Effect of GJB2 235delC and 30-35delG genetic polymorphisms on risk of congenital deafness in a Chinese population. GJB2 235delC和30-35delG基因多态性对中国人群先天性耳聋风险的影响

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019165

Y. Xiong, M. Zhong, J. Chen, Y. Yan, X. Lin, X. Li

{"title":"Effect of GJB2 235delC and 30-35delG genetic polymorphisms on risk of congenital deafness in a Chinese population.","authors":"Y. Xiong, M. Zhong, J. Chen, Y. Yan, X. Lin, X. Li","doi":"10.4238/gmr16019165","DOIUrl":"https://doi.org/10.4238/gmr16019165","url":null,"abstract":"Congenital deafness is a serious and irreversible condition in humans. The GJB2 gene is implicated in the pathogenesis of autosomal recessive nonsyndromic hearing loss. Its 235delC and 30-35delG polymorphisms are reported to be associated with risk of hereditary deafness. However, the effect of the interaction between GJB2 235delC and 30-35delG and environmental factors on congenital deafness has not been described. Therefore, we performed a case-control study to investigate the influence of these polymorphisms on congenital deafness risk, and their interaction with maternal and other environmental factors in the development of this disease. Between March 2014 and May 2015, 118 patients with congenital deafness and 242 healthy controls were enrolled into our study. Compared with the GG genotype, the adjusted odds ratios (ORs) [and 95% confidence intervals (CIs)] for the 235delC GC and CC genotypes were 4.66 (1.77-13.07) and 8.28 (2.06-47.52), respectively. Individuals harboring the GC+CC genotypes were at a greatly increased risk of congenital deafness compared to those with the GG genotype (OR = 5.65, 95%CI = 2.54-13.18). However, no significant relationship was established between the 30-35delG variant and this disease. The 235delC polymorphism exhibited an interaction with use of aminoglycoside antibiotics during pregnancy in conferring susceptibility to congenital deafness (chi-square = 8.76, P = 0.003). In conclusion, our study suggests that the GJB2 235delC polymorphism, but not the 30-35delG variant, contributes to congenital deafness susceptibility in the Chinese population examined, and demonstrates an interaction with consumption of aminoglycoside antibiotics during pregnancy in exerting this effect.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"73 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132202094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Molecular characterization, expression, and functional analysis of chicken TRAF6. 鸡TRAF6的分子特征、表达及功能分析。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019138

J. Jin, J. Ran, C. Yang, X. Jiang, Y. Zhou, Z. Feng, Y. Wang, D. Lan, P. Ren, Y. Liu

{"title":"Molecular characterization, expression, and functional analysis of chicken TRAF6.","authors":"J. Jin, J. Ran, C. Yang, X. Jiang, Y. Zhou, Z. Feng, Y. Wang, D. Lan, P. Ren, Y. Liu","doi":"10.4238/gmr16019138","DOIUrl":"https://doi.org/10.4238/gmr16019138","url":null,"abstract":"Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial adaptor molecule of the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily, which can trigger downstream signaling cascades involved in innate immunity. The function of TRAF6 has been clarified in mammals but is poorly understood in chicken. In our study, we investigated TRAF6 function in birds, particularly in chicken innate immune responses, by cloning and characterizing chicken TRAF6 (chTRAF6). The full-length coding sequence of chTRAF6 comprised 1638 bp and encoded a 545-amino acid protein, which shares high sequence similarity with TRAF6 of other species and consists of four structurally conserved domains. Quantitative real-time polymerase chain reaction revealed that chTRAF6 was widely expressed in all tested tissues and its expression was induced in chicken embryo fibroblast cells treated with poly(I:C) and poly(dA:dT). Increased expression of chTRAF6 was observed both in vitro and in vivo following infection with Newcastle disease virus in chickens. Taken together, these results suggest that chTRAF6 plays a vital role in host defense against viral infection in chicken.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126760040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption. SYBR safeTM在烟曲霉基因破坏中有效替代溴化乙啶。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019583

H. M. S. Canela, L. A. Takami, M. Ferreira

{"title":"SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption.","authors":"H. M. S. Canela, L. A. Takami, M. Ferreira","doi":"10.4238/gmr16019583","DOIUrl":"https://doi.org/10.4238/gmr16019583","url":null,"abstract":"Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable frequency. Gene disruption is essential in the search for new drug targets. An efficient protocol for A. fumigatus gene disruption was described but it requires ethidium bromide, a genotoxic agent, for DNA staining. Therefore, the present study tested SYBR safeTM, a non-genotoxic DNA stain, in A. fumigatus gene disruption protocol. The chosen gene was cipC, which has already been disrupted successfully in our laboratory. A deletion cassette was constructed in Saccharomyces cerevisiae and used in A. fumigatus transformation. There was no statistical difference between the tested DNA stains. The success rate of S. cerevisiae transformation was 63.3% for ethidium bromide and 70% for SYBR safeTM. For A. fumigatus gene disruption, the success rate for ethidium bromide was 100 and 97% for SYBR safeTM. In conclusion, SYBR safeTM efficiently replaced ethidium bromide, making this dye a safe and efficient alternative for DNA staining in A. fumigatus gene disruption.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"65 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128365845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Assessment of genetic diversity of cotton genotypes for various economic traits against cotton leaf curl disease (CLCuD). 棉花抗卷曲病(CLCuD)各经济性状基因型遗传多样性评价

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019446

M. Javed, S. Hussain, M. Baber

{"title":"Assessment of genetic diversity of cotton genotypes for various economic traits against cotton leaf curl disease (CLCuD).","authors":"M. Javed, S. Hussain, M. Baber","doi":"10.4238/gmr16019446","DOIUrl":"https://doi.org/10.4238/gmr16019446","url":null,"abstract":"In Pakistan, cotton crop has been under enormous threat of cotton leaf curl disease (CLCuD) over the last four decades. In order to estimate genetic diversity in cotton germplasm CLCuD resistance, we assessed 100 cotton genotypes for their CLCuD resistance/tolerance and other related agronomical traits. Various statistical analytical tools, including correlation analysis, cluster analysis, and principal component analysis (PCA), were used to select the best genotypes. These genotypes can be used in future breeding programs to generate CLCuD resistant varieties. The same set of procedures could be utilized for other diseases in other crops. CLCuD incidence showed a significant negative genotypic correlation with yield-contributing traits followed by a significant negative association for phenotypic correlation. The seed cotton yield showed significant positive genotypic and phenotypic correlations with plant height, number of bolls per plant, and boll weight. From the PCA we identified five principal components (PCs) that explained a significant amount of the variance among the variables, which may be used for selection of cotton genotypes with CLCuD resistance. Of the five PCs, the first four contributed more towards the total variability and had eigenvalues greater than one. The cluster analysis showed that the genotypes in one of the clusters performed particularly well with respect to CLCuD tolerance. These genotypes can be utilized for development of varieties with increased CLCuD tolerance.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132247391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Inhibition of mitochondrial calcium uptake 1 in Drosophila neurons. 果蝇神经元线粒体钙摄取1的抑制。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019436

P. G. M'Angale, B. Staveley

{"title":"Inhibition of mitochondrial calcium uptake 1 in Drosophila neurons.","authors":"P. G. M'Angale, B. Staveley","doi":"10.4238/gmr16019436","DOIUrl":"https://doi.org/10.4238/gmr16019436","url":null,"abstract":"The mitochondrial calcium uptake 1 (MICU1) is a regulatory subunit of the mitochondrial calcium uniporter that plays an important role in calcium sensing. It contains two EF-hand domains that are well conserved across diverse species from protozoa to plants and metazoans. The loss of MICU1 function in mammals is attributed to several neurological disorders that involve movement dysfunction. The CG4495 gene in Drosophila melanogaster was identified as a putative homolog of MICU1 in the HomoloGene database of the National Centre for Biotechnology Information (NCBI). In agreement with previous studies that have shown the development of neurological disorders and movement defects in MICU1 loss-of-function organisms, we attempted to identify the function of CG4495/MICU1 in Drosophila neurons. We analyzed survival and locomotor ability of these flies and additionally performed biometric analysis of the Drosophila developing eye. The inducible RNA interference-mediated inhibition of CG4495/MICU1 in the Ddc-Gal4-expressing neurons of Drosophila presented with reduction in survival coupled with a precocious loss of locomotor ability. Since the pro-survival Bcl-2 family genes have been shown to be protective towards mitochondria, and CG4495/MICU1 has a mitochondrial targeting sequence, we attempted to rescue the phenotypes resulting from the inhibition of CG4495/MICU1 by overexpressing Buffy, the sole Bcl-2 homologue in Drosophila. The co-expression of CG4495/MICU1-RNAi along with Buffy resulted in the suppression of the phenotypes induced by the inhibition of CG4495/MICU1. Subsequently, the inhibition of CG4495/MICU1 in the Drosophila developing eye, a neuron-rich organ, resulted in reduced number of ommatidia and a highly fused ommatidial array. These developmental eye defects were rescued by the overexpression of Buffy. Our study suggests an important role for MICU1 in the normal function of neurons in Drosophila.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125414379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Multivariate approach in popcorn genotypes using the Ward-MLM strategy: morpho-agronomic analysis and incidence of Fusarium spp. 利用Ward-MLM策略对爆米花基因型进行多变量分析:镰刀菌的形态农艺分析和发病率。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019528

R. N. F. Kurosawa, A. T. do Amaral Júnior, F. Silva, A. D. Dos Santos, M. Vivas, S. Kamphorst, G. Pena

{"title":"Multivariate approach in popcorn genotypes using the Ward-MLM strategy: morpho-agronomic analysis and incidence of Fusarium spp.","authors":"R. N. F. Kurosawa, A. T. do Amaral Júnior, F. Silva, A. D. Dos Santos, M. Vivas, S. Kamphorst, G. Pena","doi":"10.4238/gmr16019528","DOIUrl":"https://doi.org/10.4238/gmr16019528","url":null,"abstract":"The multivariate analyses are useful tools to estimate the genetic variability between accessions. In the breeding programs, the Ward-Modified Location Model (MLM) multivariate method has been a powerful strategy to quantify variability using quantitative and qualitative variables simultaneously. The present study was proposed in view of the dearth of information about popcorn breeding programs under a multivariate approach using the Ward-MLM methodology. The objective of this study was thus to estimate the genetic diversity among 37 genotypes of popcorn aiming to identify divergent groups associated with morpho-agronomic traits and traits related to resistance to Fusarium spp. To this end, 7 qualitative and 17 quantitative variables were analyzed. The experiment was conducted in 2014, at Universidade Estadual do Norte Fluminense, located in Campos dos Goytacazes, RJ, Brazil. The Ward-MLM strategy allowed the identification of four groups as follows: Group I with 10 genotypes, Group II with 11 genotypes, Group III with 9 genotypes, and Group IV with 7 genotypes. Group IV was distant in relation to the other groups, while groups I, II, and III were near. The crosses between genotypes from the other groups with those of group IV allow an exploitation of heterosis. The Ward-MLM strategy provided an appropriate grouping of genotypes; ear weight, ear diameter, and grain yield were the traits that most contributed to the analysis of genetic diversity.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127634037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Cloning and expression of class I chitinases in Hami melon after Penicillium infection. 感染青霉后哈密瓜I类几丁质酶的克隆与表达。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019085

F. Jiang, B. Shui, F. Tang, C. Shan

引用次数: 2