Genetics and molecular research : GMR最新文献_第4页

Migration and invasion enhancer 1 (MIEN1) is overexpressed in breast cancer and is a potential new therapeutic molecular target. 迁移和侵袭增强子1 (MIEN1)在乳腺癌中过表达，是一种潜在的新的治疗分子靶点。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019380

H.-B. Zhao, X-F Zhang, Hb Wang, M. Zhang

{"title":"Migration and invasion enhancer 1 (MIEN1) is overexpressed in breast cancer and is a potential new therapeutic molecular target.","authors":"H.-B. Zhao, X-F Zhang, Hb Wang, M. Zhang","doi":"10.4238/gmr16019380","DOIUrl":"https://doi.org/10.4238/gmr16019380","url":null,"abstract":"Migration and invasion enhancer 1 (MIEN1) is a membrane-anchored protein that is highly expressed in various types of cancer, and is correlated with the PI3K/AKT pathway. The aim of this study was to investigate the expression of MIEN1 and its clinical pathological significance in breast cancer. We used immunohistochemical staining to examine the expression of MIEN1 in 40 samples of human breast cancer tissue and 10 samples taken from regions adjacent to normal breast tissue. The rate of detection of MIEN1 protein was 67.5%, which was significantly higher than that in adjacent non-cancerous breast tissue (0%, P < 0.05). The expression of MIEN1 correlated with age, World Health Organization grade, and lymph node metastasis, but not with tumor size or family history of cancer. Kaplan-Meier survival analysis showed that patients with positive MIEN1 protein expression had a lower overall survival rate than patients who did not express MIEN1. Downregulation of MIEN1 suppressed the expression of matrix metallopeptidase 9 by downregulating the expression of protein kinase B (also known as AKT) in breast cancer cells. Our results indicate that MIEN1 overexpression may facilitate migration and invasion in breast cancer, and MIEN1 is a potential molecular target for cancer chemotherapy.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134523867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases. 巢式聚合酶链反应技术检测肝癌微转移灶中Gpc3和Afp mRNA的表达。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16018947

J. Luo, K. Yang, Y. Wen

{"title":"Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.","authors":"J. Luo, K. Yang, Y. Wen","doi":"10.4238/gmr16018947","DOIUrl":"https://doi.org/10.4238/gmr16018947","url":null,"abstract":"The incidence of liver cancer has gradually risen to a high level in China, and tumor metastasis occurs via multiple pathways. Alpha fetal protein (AFP) is the main biomarker of liver cancer micrometastases. A recent study showed that glypican-3 (GPC3), which is abundant in hepatoma cells, has promising specificity and could be used to determine the presence of malignant cells. The nested polymerase chain reaction (PCR) technique is superior in experimental sensitivity. Using rat models of liver cancer in the current study, we utilized nested PCR to detect Gpc3 and Afp mRNA to determine their relationship with liver cancer micrometastases. The aim was to provide an experimental basis for clinical examination. We randomly assigned male Sprague-Dawley rats to sham and experimental groups. The experimental group constituted a liver cancer model induced by diethylnitrosamine, whereas the sham group was administered with an equivalent volume of normal saline. Gpc3 and Afp mRNA was detected using nested PCR. Analysis was performed to determine statistical significance. Compared with the sham group, the rates of occurrence of Gpc3 and Afp mRNA were significantly higher in the experimental group (P < 0.05). Compared with the total positive ratio of hepatoma cells examined by joint detection, the rates of occurrence of Gpc3 and Afp mRNA increased significantly in the four subgroups of the experimental group (P < 0.05). The use of nested PCR significantly improved sensitivity for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132041978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Case of escape in cassava, Manihot esculenta Crantz. 木薯越狱案，马尼奥特·斯库伦塔·克兰茨。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019577

N. Nassar, M. Mendonza

引用次数: 0

Genetic tests for low- and middle-income countries: a literature review. 低收入和中等收入国家的基因检测:文献综述。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019466

P. Maltese, E. Poplavskaia, I. Malyutkina, F. Sirocco, A. Bonizzato, N. Capodicasa, S. Nicoulina, A. Salmina, N. Aksutina, Munis Dundar, T. Beccari, S. Cecchin, Matteo Bertelli

引用次数: 10

Genetic diversity among exotic cotton accessions as for qualitative and quantitative traits. 外来棉花种质间质量和数量性状的遗传多样性。

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019590

L. P. de Carvalho, F. J. C. Farias, J. Rodrigues, N. Suassuna, P. Teodoro

{"title":"Genetic diversity among exotic cotton accessions as for qualitative and quantitative traits.","authors":"L. P. de Carvalho, F. J. C. Farias, J. Rodrigues, N. Suassuna, P. Teodoro","doi":"10.4238/gmr16019590","DOIUrl":"https://doi.org/10.4238/gmr16019590","url":null,"abstract":"Studying genetic diversity among a group of genotypes is important in genetic breeding because identifying hybrid combinations of greater heterotic effect also increases the chance of obtaining plants with favorable allele combinations in an intra-population selection program. The objective of this study was to compare different types of long and extra-long staple cotton and their genetic diversity in relation to the fiber traits and some agronomic traits in order to grant breeding programs. Diversity analysis among 29 cotton accessions based on qualitative and quantitative traits and joint including qualitative and quantitative traits was performed. Analysis based on qualitative and quantitative traits and joint met the accessions in three, two, and three groups, respectively. The cross between genotypes Giza 59 and Pima unknown was the most promising to generate segregating populations, comprising simultaneously resistance (based on molecular markers) to blue disease and bacterial blight, partial resistance to root-knot nematode, smaller size, in addition to good fiber characteristics. These populations can be used in recurrent selection programs as donors of alleles for development of long-staple cotton genotypes.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122985476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Relationship between lymphocyte DNA fragmentation and dose of iron oxide (Fe2O3) and silicon oxide (SiO2) nanoparticles. 淋巴细胞DNA断裂与氧化铁(Fe2O3)和氧化硅(SiO2)纳米颗粒剂量的关系

Genetics and molecular research : GMR Pub Date : 2017-02-08 DOI: 10.4238/gmr16019206

J. Jiménez-Villarreal, D. I. Rivas-Armendáriz, R. D. A. Pérez-Vertti, E. H. O. Calderón, R. García-Garza, N. D. Betancourt-Martínez, L. Serrano-Gallardo, J. Morán-Martínez

{"title":"Relationship between lymphocyte DNA fragmentation and dose of iron oxide (Fe2O3) and silicon oxide (SiO2) nanoparticles.","authors":"J. Jiménez-Villarreal, D. I. Rivas-Armendáriz, R. D. A. Pérez-Vertti, E. H. O. Calderón, R. García-Garza, N. D. Betancourt-Martínez, L. Serrano-Gallardo, J. Morán-Martínez","doi":"10.4238/gmr16019206","DOIUrl":"https://doi.org/10.4238/gmr16019206","url":null,"abstract":"At present, the use of nanoparticles is a controversial topic, especially when analyzing their effects in human tissues. Nanoparticles (NPs) can cause oxidative stress by increasing membrane lipids peroxidation and reactive oxygen species, and decreasing intracellular glutathione. Oxidative stress plays an important role in cell signaling and inflammatory responses. It can result in genotoxicity, affect cell proliferation, and induce DNA damage. The objective of this study is to evaluate the genotoxic potential of NPs in lymphocyte DNA. Wistar female rats (N = 45) were sorted in three randomized groups as follows: Group 1 (N = 20); Group 2 (N = 20) and a control group (N = 5). A single dose of iron oxide (Fe2O3) and silicon oxide (SiO2) NPs dissolved in saline solution were administered orally to the rats. Cardiac puncture was performed to extract peripheral blood for genotoxic analysis. DNA fragmentation for lymphocytes was performed. Control rats showed a fragmentation percentage of 11.20 ± 2.16%. Rats exposed to SiO2 and Fe2O3 NPs for 24 h showed statistically significant differences in DNA fragmentation percentages as compared with that of the control group. A lineal dose-response correlation between genotoxic damage and exposure to SiO2 and Fe2O3 NPs was found (r2 = 0.99 and 0.98 for SiO2 and Fe2O3, respectively). In conclusion, we found that exposure to Fe2O3 and SiO2 NPs can cause DNA fragmentation in lymphocytes in a dose-dependent manner.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128650534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

P2X7 receptor in the hippocampus is involved in gp120-induced cognitive dysfunction. 海马P2X7受体参与gp120诱导的认知功能障碍。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019356

Y. Liu, G. Q. Chen, B. Liu, Qinkai Chen, Y. Qian, S. Qin, Chenglong Liu, Changshui Xu

{"title":"P2X7 receptor in the hippocampus is involved in gp120-induced cognitive dysfunction.","authors":"Y. Liu, G. Q. Chen, B. Liu, Qinkai Chen, Y. Qian, S. Qin, Chenglong Liu, Changshui Xu","doi":"10.4238/gmr16019356","DOIUrl":"https://doi.org/10.4238/gmr16019356","url":null,"abstract":"To investigate the role of the P2X7 receptor in learning and memory dysfunction induced by HIV-1 envelope glycoprotein gp120 (gp120), we established HIV-1-associated dementia (HAD) animal models by intracerebroventricular (ICV) infusion of gp120 in rats. We observed gp120-induced cognitive dysfunction in the radial arm water maze test. Results showed that rats in the gp120 groups had longer escape latencies and more errors compared to those in the control group. For example, the average trial time in the 50-ng/day-gp120 group on day eight (16.57 ± 1.71 s, N = 90) was significantly longer than that of control rats (9.93 ± 0.68 s, N = 90). The relative expression of P2X7 mRNA in the control, 50-, 70-, and 100-ng/day-gp120 groups were 0.43 ± 0.06, 0.48 ± 0.07, 0.83 ± 0.05, and 0.84 ± 0.10, respectively; relative P2X7 protein expression in those groups was 0.63 ± 0.07, 1.08 ± 0.06, 0.90 ± 0.07, and 1.03 ± 0.11, respectively. According to immunohistochemistry analysis, the staining intensity values for P2X7 protein expression in the control, 50-, 70-, and 100-ng/d-gp120 groups were 0.88 ± 0.07, 1.41 ± 0.12, 1.28 ± 0.13, and 1.31 ± 0.10, respectively. The above results showed that the expression of P2X7 increased significantly in the hippocampus of gp120 rats compared to that of the control group. These results suggest that ICV infusion of gp120 can successfully mimic HAD in rats, and P2X7 may be involved in gp120-induced cognitive dysfunction. This could provide a theoretical foundation and potential drug target for research and treatment of ADC.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115990099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Analysis of the genetic diversity of Lonicera japonica Thumb. using inter-simple sequence repeat markers. 金银花(Lonicera japonica拇指)遗传多样性分析。使用简单序列重复标记。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019338

H. He, D. Zhang, H. Qing, Y. Yang

引用次数: 5

A rare case of a boy with de novo microduplication at 5q35.2q35.3 from central Brazil. 来自巴西中部的一个罕见病例，在5q35.2q35.3位点有新生微复制。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019197

F. G. Reis, I. P. Pinto, L. Minasi, A. V. Melo, D. Cunha, C. L. Ribeiro, C. C. da Silva, D. Silva, A. D. da Cruz

{"title":"A rare case of a boy with de novo microduplication at 5q35.2q35.3 from central Brazil.","authors":"F. G. Reis, I. P. Pinto, L. Minasi, A. V. Melo, D. Cunha, C. L. Ribeiro, C. C. da Silva, D. Silva, A. D. da Cruz","doi":"10.4238/gmr16019197","DOIUrl":"https://doi.org/10.4238/gmr16019197","url":null,"abstract":"Genomic disorders are genetic diseases that are caused by rearrangements of chromosomal material via deletions, duplications, and inversions of unique genomic segments at specific regions. Such rearrangements could result from recurrent non-allelic homologous recombination between low copy repeats. In cases where the breakpoints flank the low copy repeats, deletion of chromosomal segments is often followed by reciprocal duplication. Variations in genomic copy number manifest differently, with duplication and deletions of the same genomic region showing opposite phenotypes. Sotos syndrome is caused by alterations in the dosage of NSD1 on human chromosome 5 by either deletions or mutations, such as microdeletion of 5q35.2q35.3. In general, patients carrying reciprocal microduplication at 5q35.2q35.3 present no clinical phenotype or milder phenotype than do patients with microdeletion at the same locus. We report the first case of 5q35.2q35.3 microduplication encompassing NSD1 in a patient from central Brazil. We identified a genomic imbalance corresponding to a de novo 0.45 Mb microduplication at 5q35.2q35.3 by chromosomal microarray analysis and study of low-copy repeats. The proband had microduplication in the chromosomal region containing NSD1, which resulted in a Sotos syndrome reversed phenotype, and this duplication was associated with microcephaly, short stature, and developmental delay. Analysis of the genomic structure of the rearranged 5q35.2q35.3 chromosomal region revealed two major low-copy repeat families, which caused the recurrent rearrangements. Chromosomal microarray analysis is a potential tool to identify microrearrangements and guide medical diagnosis, which has to be followed by a non-directive genetic counseling approach to improve the quality of life of the patient.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"16 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130933810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prevalence of thymidylate synthase gene 5'-untranslated region variants in an Argentinean sample. 胸苷酸合成酶基因5'-未翻译区变异在阿根廷样本中的流行。

Genetics and molecular research : GMR Pub Date : 2017-01-23 DOI: 10.4238/gmr16019367

Carolina Vázquez, M. Orlova, P. Scibona, H. D. D. Arce, M. Pallotta, W. Belloso

{"title":"Prevalence of thymidylate synthase gene 5'-untranslated region variants in an Argentinean sample.","authors":"Carolina Vázquez, M. Orlova, P. Scibona, H. D. D. Arce, M. Pallotta, W. Belloso","doi":"10.4238/gmr16019367","DOIUrl":"https://doi.org/10.4238/gmr16019367","url":null,"abstract":"Thymidylate synthase (TYMS) is a key enzyme in nucleotide synthesis and therefore, an important target of many chemotherapeutic agents. Expression of TYMS mRNA is thought to be modulated by a 28-bp tandem repeat polymorphism within its 5'-untranslated region, raising the question of this variant's utility in predicting the efficacy and toxicity of cancer treatment regimens. The aim of the present research was to describe the distribution of this TYMS polymorphism in the Argentinean population. A total of 199 randomly selected DNA samples from healthy volunteers were analyzed using polymerase chain reaction and polyacrylamide gel electrophoresis. The 2R and 3R alleles were present in 47.74 and 52.26% of samples, respectively, with frequencies of 21.6 (43), 52.3 (104), and 26.1% (52) recorded for the 2R/2R, 2R/3R, and 3R/3R genotypes, respectively. No significant difference regarding gender was observed. Our prevalence data are similar to those reported for other Caucasian populations. This opens a discussion concerning the reference population valid for comparisons and the clinical importance of this genotyping test as an additional tool in personalized medicine.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132454031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2