Genetics and molecular research : GMR最新文献

IL-8 -251A/T polymorphism contributes to coronary artery disease susceptibility in a Chinese population. IL-8 -251A/T多态性与中国人群冠状动脉疾病易感性有关

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16018224

R. Zhang, X. D. Li, S. Zhang, X. H. Li, L. Wu

{"title":"IL-8 -251A/T polymorphism contributes to coronary artery disease susceptibility in a Chinese population.","authors":"R. Zhang, X. D. Li, S. Zhang, X. H. Li, L. Wu","doi":"10.4238/gmr16018224","DOIUrl":"https://doi.org/10.4238/gmr16018224","url":null,"abstract":"Interleukin-8 (IL-8) is a mediator of inflammation and plays an important role in regulating immune responses. To date, several studies have tested the association between IL-8 gene polymorphisms and development of coronary artery disease (CAD), but their results have proved to be inconsistent. We conducted an investigation to assess the relationship between the IL-8 -251A/T (rs4073) sequence variant and CAD in a Chinese population. Between April 2013 and January 2015, 217 patients with coronary angiography-confirmed CAD were enrolled in our study, along with 245 control subjects. IL-8 -251A/T genotyping was performed using a polymerase chain reaction-restriction fragment length polymorphism assay. A chi-square test revealed that IL-8 -251A/T genotype distributions significantly differed between CAD patients and control subjects (chi-square = 8.29, P < 0.02). Moreover, multiple-logistic regression analysis showed that individuals carrying TA [odds ratio (OR) = 1.59, 95% confidence interval (CI) = 1.01-2.57] and AA (OR = 2.06, 95%CI = 1.21-3.52) genotypes were at increased risk of CAD compared to those with the TT genotype. Under dominant (OR = 1.75, 95%CI = 1.13-2.73) and recessive (OR = 1.54, 95%CI = 1.02-2.37) genetic models, the IL-8 -251A/T polymorphism also significantly correlated with CAD. In conclusion, our results suggest that this variant is an independent risk factor for CAD development under codominant, dominant, and recessive models.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122148349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Identification and cloning of GOLDEN2-LIKE1 (GLK1), a transcription factor associated with chloroplast development in Brassica napus L. 甘蓝型油菜叶绿体发育相关转录因子GLK1的鉴定与克隆。

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16018942

Y. Pan, Y. Pan, C. Qu, C. Su, J. Li, X. Zhang

{"title":"Identification and cloning of GOLDEN2-LIKE1 (GLK1), a transcription factor associated with chloroplast development in Brassica napus L.","authors":"Y. Pan, Y. Pan, C. Qu, C. Su, J. Li, X. Zhang","doi":"10.4238/gmr16018942","DOIUrl":"https://doi.org/10.4238/gmr16018942","url":null,"abstract":"Photosynthesis is the process by which dry matter accumulates, which affects rapeseed yield. In this study, we identified GOLDEN2-LIKE1 (GLK1), located on chromosome A07 and 59.2 kb away from the single nucleotide polymorphism marker SNP16353A07, which encodes a transcription factor associated with the rate of photosynthesis in leaves. We then identified 96 GLK1 family members from 53 species using a hidden Markov model (HMM) search and found 24 of these genes, which were derived from 17 Brassicaceae species. Phylogenetic analysis showed that 24 Brassicaceae proteins were classified into three subgroups, named the Brassica family, Adenium arabicum, and Arabidopsis. Using homologous cloning methods, we identified four BnaGLK1 copies; however, the coding sequences were shorter than the putative sequences from the reference genome, probably due to splicing errors among the reference genome sequence or different gene copies being present in the different B. napus lines. In addition, we found that BnaGLK1 genes were expressed at higher levels in leaves with more chloroplasts than were present in other leaves. Overexpression of BnaGLK1a resulted in darker leaves and siliques than observed in the control, suggesting that BnaGLK1 might promote chloroplast development to affect the rate of photosynthesis in leaves. These results will help to elucidate the mechanism of chloroplast biogenesis by GLK1 in B. napus.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132176516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Polyandrous behavior in an overexploited giant South American turtle (Podocnemis expansa) population in Central Amazon, Brazil. 巴西亚马逊中部过度开发的巨型南美龟(Podocnemis expansa)种群的多雄行为。

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16019537

C. Fantin, J. Morais, R. Botero-Arias, C. Araújo, C. Camillo, I. Farias

引用次数: 1

Tumor-suppressive role of Kruppel-like factor 4 (KLF-4) in colorectal cancer. kruppel样因子4 (KLF-4)在结直肠癌中的抑瘤作用。

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16019272

D. Xiu, Y. Chen, L. Liu, H. Yang, G. F. Liu

{"title":"Tumor-suppressive role of Kruppel-like factor 4 (KLF-4) in colorectal cancer.","authors":"D. Xiu, Y. Chen, L. Liu, H. Yang, G. F. Liu","doi":"10.4238/gmr16019272","DOIUrl":"https://doi.org/10.4238/gmr16019272","url":null,"abstract":"Kruppel-like factors (KLFs) are a group of transcriptional regulators that have recently been identified to exhibit tumor-suppressive function against various gastrointestinal cancers. The present study aims to investigate the expression patterns and prognostic value of KLF-4 in colorectal cancers (CRCs). KLF-4 levels in CRC tissues were examined via immunohistochemistry analysis, real-time quantitative polymerase chain reaction, and western blotting. The chi-square test was performed to evaluate the correlation between KLF-4 expression and the clinicopathological characteristics. Kaplan-Meier analysis was performed to assess the prognostic value of KLF-4 in CRC patients. In addition, we evaluated the effect of KLF-4 knockdown on the proliferation of CRC HT-29 cells. Our results showed significant downregulation of KLF-4 in 31 CRC samples, collected from CRC patients showing more malignant characteristics such as lymphatic metastasis, low tumor cell differentiation, and tumor recurrence. CRC patients in the low KLF-4 group were found to have reduced overall survival and decreased disease-free survival time. Moreover, HT-29 cells transfected with siRNA-KLF-4 showed increased proliferation compared to those transfected with control siRNA. In summary, lower KLF-4 expression was correlated with malignant CRC status and poor prognosis in CRC patients. Moreover, KLF-4 suppression promoted the proliferation of CRC cells in vitro. These results provide novel insights into the tumor suppressive role of KLF-4 in CRC.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122026092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Study on the effects of blueberry treatment on histone acetylation modification of CCl4-induced liver disease in rats. 蓝莓对ccl4致大鼠肝病组蛋白乙酰化修饰的影响研究。

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16019188

W. Zhan, X. Liao, T. Tian, Lei Yu, X. Liu, B. Li, Jianjun Liu, B. Han, R. Xie, Q. Ji, Qian Yang

{"title":"Study on the effects of blueberry treatment on histone acetylation modification of CCl4-induced liver disease in rats.","authors":"W. Zhan, X. Liao, T. Tian, Lei Yu, X. Liu, B. Li, Jianjun Liu, B. Han, R. Xie, Q. Ji, Qian Yang","doi":"10.4238/gmr16019188","DOIUrl":"https://doi.org/10.4238/gmr16019188","url":null,"abstract":"The objective of this study was to investigate the effects of blueberry treatment on histone acetylation modification of carbon tetrachloride (CCl4)-induced liver disease in rats. Laboratory rats were randomly divided into control, hepatic fibrosis, blueberry treatment, blueberry intervention, and natural recovery groups. Rats in the model groups were treated with CCl4 administered subcutaneously at 4- and 8-week intervals, and then executed. Both the 4- and 8-week treatment groups were treated with blueberry juice for 8 weeks, and then executed after 12 and 16 weeks, respectively. Following the experiment, four liver function and hepatic fibrosis indices were measured. Liver index was calculated, hematoxylin-eosin staining was conducted, and H3K9, H3K14, and H3K18 expressions were evaluated among the nuclear proteins of the liver tissues. No differences in alanine transaminase were noted between the control and intervention groups, but significant differences were detected among the model, treatment, and natural recovery groups (P < 0.01). Significant differences were also observed in aspartate transaminase, hyaluronic acid, and collagen IV among the model, treatment, intervention, and natural recovery groups (P < 0.01, P < 0.01, P < 0.01). Liver index, and H3K9 and H3K14 expression were significantly different among the model groups (P < 0.05 and P < 0.01), whereas H3K18 expression was dramatically different among model, treatment, intervention, and natural recovery groups (P < 0.01). Following blueberry treatment, rat liver function and hepatic fibrosis improved, potentially indicating that blueberry components could regulate histone acetylation and improve liver pathologic changes in rats with CCl4-induced disease.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122789119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Genotypic and allelic frequencies of gene polymorphisms associated with meat tenderness in Nellore beef cattle. 内洛尔肉牛肉质嫩度相关基因多态性的基因型和等位基因频率。

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16018957

M. Carvalho, J. Eler, M. Bonin, F. Rezende, Fernando H. Biase, Flávio Vieira Meirelles, L. Regitano, Luiz Lehmann Coutinho, J. C. D. C. Balieiro, José Bento Sterman Ferraz

{"title":"Genotypic and allelic frequencies of gene polymorphisms associated with meat tenderness in Nellore beef cattle.","authors":"M. Carvalho, J. Eler, M. Bonin, F. Rezende, Fernando H. Biase, Flávio Vieira Meirelles, L. Regitano, Luiz Lehmann Coutinho, J. C. D. C. Balieiro, José Bento Sterman Ferraz","doi":"10.4238/gmr16018957","DOIUrl":"https://doi.org/10.4238/gmr16018957","url":null,"abstract":"The objectives of this study were to characterize the allelic and genotypic frequencies of polymorphisms in the µ-calpain and calpastatin genes, and to assess their association with meat tenderness and animal growth in Nellore cattle. We evaluated 605 Nellore animals at 24 months of age, on average, at slaughter. The polymorphisms were determined for the molecular markers CAPN316, CAPN530, CAPN4751, CAPN4753, and UOGACAST1. Analyses of meat tenderness at 7, 14, and 21 days of maturation were performed in samples of longissimus thoracis obtained between the 12th and 13th rib and sheared using a Warner Bratzler Shear Force. Significant effects were observed for meat tenderness at days 7, 14, and 21 of maturation for the marker CAPN4751, at day 21 for the marker CAPN4753, and at days 14 and 21 for the marker UOGCAST1. For genotypic combinations of markers, the results were significant for the combination CAPN4751/UOGCAST1 in the three maturation periods and CAPN4753/UOGCAST1 at days 14 and 21 of maturation.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122865031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 98

Chloroplast diversity of Casearia grandiflora in the Cerrado of Piauí State. Piauí州塞拉多地区大花Casearia grandflora叶绿体多样性研究。

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16019572

M. Costa, A. A. Pereira, J. B. Pinheiro, M. Zucchi, A. Araújo, R. L. F. Gomes, S. Valente, M. A. Oliveira, A. Lopes

{"title":"Chloroplast diversity of Casearia grandiflora in the Cerrado of Piauí State.","authors":"M. Costa, A. A. Pereira, J. B. Pinheiro, M. Zucchi, A. Araújo, R. L. F. Gomes, S. Valente, M. A. Oliveira, A. Lopes","doi":"10.4238/gmr16019572","DOIUrl":"https://doi.org/10.4238/gmr16019572","url":null,"abstract":"Casearia grandiflora (Salicaceae) is a typical Cerrado species adapted to disturbed environments, making it useful for restoration projects. Knowledge of genetic diversity is important for establishing conservation strategies for this species. This study aimed to compare chloroplast haplotype diversity and structure of C. grandiflora, under the assumption that protected areas hold greater genetic diversity than disturbed areas. The populations studied are from Parque Nacional de Sete Cidades Conservation Unit and from the surroundings of the city of Cocal de Telha, both located in the State of Piauí. Molecular analyses were performed with seven chloroplast microsatellite loci. The number of private haplotypes and haplotype diversity were higher in the conservation unit, which reinforces the importance of these areas in maintaining biodiversity. Analysis of molecular variance showed that most of the genetic variation is found within populations, with a moderate divergence between them (FST = 0.14). The Bayesian analysis and discriminant analysis of principal components suggested that populations are not structured, revealing that a set of individuals from Parque Nacional de Sete Cidades were more divergent within populations than between them. Since literature has little information on C. grandiflora, the results of this study provide important contribution to a better understanding of the specie's genetic diversity.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"2017 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121451608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibition of SHH pathway mechanisms by arsenic trioxide in pediatric medulloblastomas: a comprehensive literature review. 三氧化二砷对儿童髓母细胞瘤SHH通路抑制机制的综合文献综述

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16019412

P. Klinger, A. Andrade, L. E. Delsin, R. P. Queiroz, C. Scrideli, L. Tone, E. Valera

引用次数: 6

Genetic relationships in a germplasm collection of Camellia japonica and Camellia oleifera using SSR analysis. 利用SSR分析日本山茶和油茶种质资源的亲缘关系。

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16019526

Y. Zhao, C. Ruan, G. Ding, S. Mopper

{"title":"Genetic relationships in a germplasm collection of Camellia japonica and Camellia oleifera using SSR analysis.","authors":"Y. Zhao, C. Ruan, G. Ding, S. Mopper","doi":"10.4238/gmr16019526","DOIUrl":"https://doi.org/10.4238/gmr16019526","url":null,"abstract":"Camellia japonica produces different color and bigger flowers, widely being used for gardening green in southern China. However, cultivars were introduced from different regions, but their origin and pedigree information is either not available poorly documented, causing problems in authentication. Many low-yield trees in Camellia oleifera forests have been used as stocks for grafting C. japonica. However, the survival rate of grafts between these two species is related to genetic relationship between stock of C. oleifera and scion of C. japonica. We used simple sequence repeat (SSR) markers to genotype 41 C. japonica cultivars from different regions, as well as nine genotypes of C. oleifera in China. Twenty-one SSR markers generated 438 alleles, with an average of 20.85 alleles per locus. All alleles were used to generate Dice coefficients between two genotypes of all genotypes of these two species. Cluster analysis based on SSR data clustered genotypes showed clustering of genotypes into groups that agreed well with their taxonomic classification and geographic origin. Cultivar 'Damaonao' was a large tree with flowers of composite color, and showed the most genetic distance from other C. japonica cultivars and C. oleifera genotypes in the cluster analysis. The cultivars of C. japonica are distinct from genotypes of C. oleifera. The results for cultivars of C. japonica also revealed the presence of different cultivars with the same name, and identical cultivars but with a different name. SSR profiles can improve C. japonica germplasm management, and provide potential determine correlations between genetic relationship and graft compatibility among scions of C. japonica and genotypes of C. oleifera.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"175 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116979275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

MicroRNA-139 modulates Alzheimer's-associated pathogenesis in SAMP8 mice by targeting cannabinoid receptor type 2. MicroRNA-139通过靶向大麻素受体2型调节SAMP8小鼠阿尔茨海默病相关发病机制

Genetics and molecular research : GMR Pub Date : 2017-02-16 DOI: 10.4238/gmr16019166

Y. Tang, J. Bao, J. Su, W. Huang

{"title":"MicroRNA-139 modulates Alzheimer's-associated pathogenesis in SAMP8 mice by targeting cannabinoid receptor type 2.","authors":"Y. Tang, J. Bao, J. Su, W. Huang","doi":"10.4238/gmr16019166","DOIUrl":"https://doi.org/10.4238/gmr16019166","url":null,"abstract":"Alzheimer's disease (AD) is a neurodegenerative disorder, and is the most common type of dementia in the elderly population. Growing evidence indicates that microRNAs (miRNAs) play a crucial role in neuroinflammation associated with AD progression. In this study, we analyzed the expression of microRNA-139 (miR-139) as well as the learning and memory function in AD. We observed that the miR-139 expression was significantly higher in the hippocampus of aged senescence accelerated mouse prone 8 (SAMP8) mice (2.92 ± 0.13) than in the control mice (1.49 ± 0.08). Likewise, the overexpression of miR-139 by means of hippocampal injection impaired the hippocampus-dependent learning and memory formation. In contrast, the downregulation of miR-139 in mice improved learning and memory function in the mice. The level of cannabinoid receptor type 2 (CB2), a potential target gene of miR-139, was inversely correlated with the miR-139 expression in primary hippocampal cells. Furthermore, we demonstrated that miR-139 inversely modulated the responses to proinflammatory stimuli. Together, our findings demonstrate that miR-139 exerts a pathogenic effect in AD by modulating CB2-meditated neuroinflammatory processes.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127370587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30