感染青霉后哈密瓜I类几丁质酶的克隆与表达。

F. Jiang, B. Shui, F. Tang, C. Shan
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引用次数: 2

摘要

几丁质酶是一种重要的疾病相关蛋白,在植物抗病过程中起着重要作用。为研究几丁质酶在哈密瓜抗青霉感染中的作用,克隆了几丁质酶基因HmCHT-2,并采用RT-PCR技术检测其表达水平。结果表明,与对照组相比,哈密瓜感染青霉菌0、12、36、48、60和72 h后表达量达到峰值的时间延迟,表达量维持在显著高水平的时间延长。这些结果提示HmCHT-2可能参与了哈密瓜抗真菌感染的防御作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cloning and expression of class I chitinases in Hami melon after Penicillium infection.
Chitinases are important disease-related proteins that play critical roles in plant defense against disease. To investigate the function of chitinases in the resistance of Hami melon to Penicillium infection, the gene encoding chitinases, HmCHT-2, was cloned and RT-PCR was used to measure expression levels of HmCHT-2. When the Hami melon was infected by Penicillium sp after 0, 12, 36, 48, 60, and 72 h. The results showed that comparing to the control group, the time of expression levels reaching to the peak delayed and the expression levels maintained at a significantly high level for a longer time. These results suggest that HmCHT-2 may contribute to the defense of Hami melon against fungal infection.
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