Molecular Reproduction and Development最新文献_第7页

lncRNA GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in pre-eclampsia lncRNA GHET1通过EZH2/LSD1介导的子痫前期MT2A表观遗传学抑制调节绒毛外滋养层表型。

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-08-07 DOI: 10.1002/mrd.23693

Pengyun Wan, Jia Huang, Wenting Liu, Xiaoyan Su, Bei Zhao, Xianggang Wang, Lu Zhao

{"title":"lncRNA GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in pre-eclampsia","authors":"Pengyun Wan, Jia Huang, Wenting Liu, Xiaoyan Su, Bei Zhao, Xianggang Wang, Lu Zhao","doi":"10.1002/mrd.23693","DOIUrl":"10.1002/mrd.23693","url":null,"abstract":"Pre-eclampsia (PE) is usually defined as new-onset hypertension with albuminuria or other organ damage. Herein, the role and mechanism of long noncoding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) during PE are investigated. Expression of GHET1 in PE pregnancies was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation and cell cycle of extravillous trophoblasts were assessed by Cell Counting Kit-8 (CCK-8), colony formation, 5-Ethynyl-2′-deoxyuridine (EdU) assays, and flow cytometry, respectively. Migration, invasion, and network formation of trophoblasts were measured by wound healing, transwell system, and tube formation assays. RNA immunoprecipitation (RIP), RNA pull-down, and chromatin immunoprecipitation (ChIP) assays were used to confirm the molecular interaction. GHET1 was markedly decreased in the placenta of PE patients. GHET1 promoted the proliferation and cell cycle of extravillous trophoblasts, as well as migration, invasion, and network formation in vitro. Metallothionein 2A (MT2A) functioned as a downstream effector of GHET1, which was negatively correlated with GHET1 in PE. GHET1 directly bound with zeste 2 polycomb repressive complex 2/lysine-specific demethylase 1 (EZH2/LSD1). Knockdown of GHET1 reduced the occupancies of H3K27me3 and H3K4me2 in the MT2A promoter region by recruiting EZH2 and LSD1. MT2A knockdown reversed GHET1 inhibition mediated biological functions. GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in PE.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 8-9","pages":"758-770"},"PeriodicalIF":2.5,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10000647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of phosphodiesterase PDE8B reduces activation of primordial follicles in mouse ovaries 抑制磷酸二酯酶PDE8B可降低小鼠卵巢中原始卵泡的激活

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-07-27 DOI: 10.1002/mrd.23699

Julie Feld Madsen, Mahboobeh Amoushahi, Christian Posselt Choi, Stine Bundgaard, Anders Heuck, Karin Lykke-Hartmann

{"title":"Inhibition of phosphodiesterase PDE8B reduces activation of primordial follicles in mouse ovaries","authors":"Julie Feld Madsen, Mahboobeh Amoushahi, Christian Posselt Choi, Stine Bundgaard, Anders Heuck, Karin Lykke-Hartmann","doi":"10.1002/mrd.23699","DOIUrl":"10.1002/mrd.23699","url":null,"abstract":"In the ovaries, cyclic adenosine 3′,5′-monophosphate (cAMP) is a second messenger supporting the generation of steroids. Phosphodiesterases (PDEs) are regulators of intracellular cAMP, and therefore, potential regulators of ovarian function. Interestingly, the family of PDE genes are differentially expressed in human oocytes and granulosa cells from primordial and primary follicles, suggesting diverse roles. In this study, we addressed the functions of PDE3B and PDE8B in primordial follicle regulation using inhibitors of PDE3B and PDE8B in murine ovary primary in vitro cultures. Inhibition of PDE8B in ovarian cultures prevented primordial follicle activation, while inhibition of PDE3B had no effect on follicle distribution in the ovary, under the tested conditions. As cAMP levels may increase steroid levels, we assessed the protein levels of the steroidogenic acute regulatory protein (StAR) and aromatase enzymes, and found that inhibition of PDE3B reduced StAR protein levels, whereas inhibition of PDE8 did not alter StAR expression in our murine ovary culture system conditions. Our results showed that ketotifen-induced inhibition of PDE8B can decrease primordial follicle activation, whereas we observed no effect of follicle distribution, when PDE3B was inhibited. Expression of the StaR enzyme was not altered when PDE8B was inhibited, which might reflect not sufficient inhibition by ketotifen to induce StAR alterations, or redundant mechanisms.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 6","pages":"378-388"},"PeriodicalIF":2.5,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23699","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9955786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The expression levels of NOS2, HMOX1, and VEGFC in cumulus cells are markers of oocyte maturation and fertilization rate 卵丘细胞中NOS2、HMOX1和VEGFC的表达水平是卵母细胞成熟和受精率的标志

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-07-24 DOI: 10.1002/mrd.23698

Montserrat Barragán, David Cornet-Bartolomé, Natalia Molina, Rita Vassena

{"title":"The expression levels of NOS2, HMOX1, and VEGFC in cumulus cells are markers of oocyte maturation and fertilization rate","authors":"Montserrat Barragán, David Cornet-Bartolomé, Natalia Molina, Rita Vassena","doi":"10.1002/mrd.23698","DOIUrl":"10.1002/mrd.23698","url":null,"abstract":"Throughout the reproductive life of women, cumulus cells (CC) protect the dormant oocyte from damage, act as sensors of the follicular microenvironment, and act as a gatekeeper for oocyte developmental potential. One such mechanism relies on the hypoxia-tolerance response, which, with age, decreases systematically, including in the ovary. We aimed to evaluate the association between gene expression related to hypoxia and aging in CC and reproductive results in in vitro fertilization cycles. We recruited 94 women undergoing controlled ovarian stimulation. Total RNA was extracted from pooled CCs collected after oocyte pick-up (OPU) and reverse-transcribed to complementary DNA using random hexamers to test 14 genes related to hypoxia response via HIF1α activation, oxidative stress, and angiogenic responses. The expression of CLU, NOS2, and TXNIP had a positive correlation with age (rs = 0.25, rs = 0.24, and rs = 0.35, respectively). Additionally, NOS2 and HMOX1 expression correlated positively with the retrieval of immature oocytes (rs = 0.22 and rs = 0.40, respectively). Moreover, VEGFC levels decreased overall with increasing fertilization rate, independently of age (rs = −0.29). We found that the fertilization potential of a cohort of oocytes is related to the ability of CC to respond to oxidative stress and hypoxia with age, pointing at NOS2, HMOX1, and VEGFC expression as markers for oocyte maturation and fertilization success.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 6","pages":"369-377"},"PeriodicalIF":2.5,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9955782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hsa_circ_0001740 mediates trophoblast cell function via regulating miR-188-3p/ARRDC3 Hsa_circ_0001740通过调节miR-188-3p/ARRDC3介导滋养细胞功能

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-07-12 DOI: 10.1002/mrd.23695

Mei Long, Shan Wang

{"title":"Hsa_circ_0001740 mediates trophoblast cell function via regulating miR-188-3p/ARRDC3","authors":"Mei Long, Shan Wang","doi":"10.1002/mrd.23695","DOIUrl":"10.1002/mrd.23695","url":null,"abstract":"Preeclampsia is an obstetric disorder and remains the leading contributor to maternal and fetal morbidity and mortality. This study was designed to explore the role of hsa_circ_0001740 in preeclampsia as well as its underlying mechanism. Real-time quantitative polymerase chain reaction was performed to examine hsa_circ_0001740 and miR-188-3p levels in trophoblast cell line HTR-8/SVneo. The proliferation, migration, invasion, and apoptosis of HTR-8/SVneo cells were detected using cell counting kit-8, colony formation, wound healing, transwell, and terminal-deoxynucleoitidyl transferase mediated nick end labeling assays, respectively. The expression of apoptosis- and Hippo signaling-related proteins were assessed by western blot. Moreover, the binding relationship between hsa_circ_0001740 and miR-188-3p, miR-188-3p and ARRDC3 were verified by luciferase report assay. The results showed that hsa_circ_001740 overexpression inhibited the proliferation, migration, and invasion, and promoted apoptosis of HTR-8/SVneo cells. Hsa_circ_0001740 was verified to bind to miR-188-3p, and ARRDC3 was demonstrated to be a target of miR-188-3p. miR-188-3p overexpression partially counteracted the suppressive effects of hsa_circ_001740 overexpression on the proliferation, migration, and invasion of HTR-8/SVneo cells. What's more, ARRDC3 expression was upregulated by hsa_circ_001740-overexpression but was downregulated by miR-188-3p overexpression. Hsa_circ_001740/miR-188-3p also mediated Hippo signaling. To summarize, hsa_circ_0001740 could maintain trophoblast cell function via downregulating miR-188-3p, providing a potential biomarker for the diagnosis and treatment of preeclampsia.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 6","pages":"406-416"},"PeriodicalIF":2.5,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9952792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased LONP1 expression contributes to DNA damage and meiotic defects in oocytes 降低LONP1表达有助于卵母细胞DNA损伤和减数分裂缺陷

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-07-01 DOI: 10.1002/mrd.23694

Chuanming Liu, Manlin Xu, Yajie Guan, Lilin Li, Wenwen Liu, Bichun Guo, Xiaoqiang Sheng, Yang Zhang, Jidong Zhou, Xin Zhen, Guijun Yan, Haixiang Sun, Lijun Ding

引用次数: 0

The constitutively active pSMAD2/3 relatively improves the proliferation of chicken primordial germ cells 组成活性的pSMAD2/3相对促进鸡原始生殖细胞的增殖

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-06-28 DOI: 10.1002/mrd.23689

Masumeh Zare, Seyed Ziaeddin Mirhoseini, Shahrokh Ghovvati, Saeed Yakhkeshi, Mahdi Hesaraki, Mojgan Barati, Forough Azam Sayyahpour, Hossein Baharvand, Seyedeh-Nafiseh Hassani

{"title":"The constitutively active pSMAD2/3 relatively improves the proliferation of chicken primordial germ cells","authors":"Masumeh Zare, Seyed Ziaeddin Mirhoseini, Shahrokh Ghovvati, Saeed Yakhkeshi, Mahdi Hesaraki, Mojgan Barati, Forough Azam Sayyahpour, Hossein Baharvand, Seyedeh-Nafiseh Hassani","doi":"10.1002/mrd.23689","DOIUrl":"10.1002/mrd.23689","url":null,"abstract":"In many multicellular organisms, mature gametes originate from primordial germ cells (PGCs). Improvements in the culture of PGCs are important not only for developmental biology research, but also for preserving endangered species, and for genome editing and transgenic animal technologies. SMAD2/3 appear to be powerful regulators of gene expression; however, their potential positive impact on the regulation of PGC proliferation has not been taken into consideration. Here, the effect of TGF-β signaling as the upstream activator of SMAD2/3 transcription factors was evaluated on chicken PGCs' proliferation. For this, chicken PGCs at stages 26−28 Hamburger−Hamilton were obtained from the embryonic gonadal regions and cultured on different feeders or feeder-free substrates. The results showed that TGF-β signaling agonists (IDE1 and Activin-A) improved PGC proliferation to some extent while treatment with SB431542, the antagonist of TGF-β, disrupted PGCs' proliferation. However, the transfection of PGCs with constitutively active SMAD2/3 (SMAD2/3CA) resulted in improved PGC proliferation for more than 5 weeks. The results confirmed the interactions between overexpressed SMAD2/3CA and pluripotency-associated genes NANOG, OCT4, and SOX2. According to the results, the application of SMAD2/3CA could represent a step toward achieving an efficient expansion of avian PGCs.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 6","pages":"339-357"},"PeriodicalIF":2.5,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9952760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-lapse monitoring: An adjunct tool to select embryos for preimplantation genetic testing 延时监测:用于胚胎着床前基因检测的辅助工具

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-06-19 DOI: 10.1002/mrd.23692

Daniela P. de Almeida Ferreira Braga, Amanda S. Setti, Patricia Guilherme, Christina Morishima, Assumpto Iaconelli Jr., Edson Borges Jr.

{"title":"Time-lapse monitoring: An adjunct tool to select embryos for preimplantation genetic testing","authors":"Daniela P. de Almeida Ferreira Braga, Amanda S. Setti, Patricia Guilherme, Christina Morishima, Assumpto Iaconelli Jr., Edson Borges Jr.","doi":"10.1002/mrd.23692","DOIUrl":"10.1002/mrd.23692","url":null,"abstract":"The goal for the present study was to investigate the effect of aneuploidy on embryo morphokinetics events in a time-lapse imaging (TLI) system incubator. This retrospective cohort study was performed in a private university-affiliated in vitro fertilization center, between 2019 March and December 2020. Kinetic data were analyzed in 935 embryos, derived from 316 patients undergoing intracytoplasmic sperm injection cycle with preimplantation genetic testing (PGT) for aneuploidy, individually cultured in a TLI incubator until Day 5 of development. Timing of morphokinetic variables, the incidences of multinucleation, and Known Implantation Data Score (KIDScore)-Day 5 were compared between euploid (n = 352) and aneuploid embryos (n = 583). Aneuploid embryos showed significantly longer timing to complete specific morphokinetic parameters compared to euploidy embryos. Euploidy embryos also showed a significantly higher KIDScore when compared with the aneuploidy ones. Our evidence suggests that TLI monitoring may be an adjunct approach to select embryos for PGT; however, cautious investigation is still needed.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 6","pages":"389-396"},"PeriodicalIF":2.5,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10322001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm capacitation as a predictor of boar fertility 精子获能作为野猪生育能力的预测因子

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-06-12 DOI: 10.1002/mrd.23690

Alexandra Keller, Karl Kerns

引用次数: 0

Mitochondrial energy metabolism is downregulated in repeated implantation failure patients related to alteration of PGC-1α acetylation level 反复植入失败患者线粒体能量代谢下调与PGC-1α乙酰化水平改变有关

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-05-27 DOI: 10.1002/mrd.23691

Shuo Han, Sai Liu, Nai-Qian Jin, Ya-Su Lv, Mo Yang, Shuai Ma, Yao Fu, Shan-Ke Zhao, Ming-Hui Liu

{"title":"Mitochondrial energy metabolism is downregulated in repeated implantation failure patients related to alteration of PGC-1α acetylation level","authors":"Shuo Han, Sai Liu, Nai-Qian Jin, Ya-Su Lv, Mo Yang, Shuai Ma, Yao Fu, Shan-Ke Zhao, Ming-Hui Liu","doi":"10.1002/mrd.23691","DOIUrl":"10.1002/mrd.23691","url":null,"abstract":"Herein we aimed at exploring mitochondrial energy metabolism status in patients with repeated implantation failure (RIF) and whether key regulatory factor PGC-1α of energy metabolism is involved in the decidualization of endometrial stromal cells. Mitochondrial oxidative phosphorylation level and ATP synthesis were compared in primary endometrial stromal cells from RIF and control group. At the same time, as one of key transcription regulators of mitochondrial energy metabolism, the expression level and acetylation level of PGC-1α were compared with two groups. Then, we downregulated the acetylation levels of PGC-1α, and the expression of decidual markers (PRL and IGFBP1) was observed further. Mitochondrial energy metabolism, showing by mitochondrial oxidative phosphorylation level and ATP synthesis, was decreased in the endometrial stromal cells of the RIF group (RIF-hEnSCs). Meanwhile, PGC-1α acetylation levels were significantly higher in RIF-hEnSCs. When we reduced the acetylation levels of PGC-1α in RIF-hEnSCs, the basal O2 consumption rate and maximal respiration were increased, and also the PRL and IGFBP1. Overall, our data indicated that the endometrial stromal cells of the RIF patients had low level of mitochondrial energy metabolism. Reducing acetylation level of key energy metabolism regulator PGC-1α can increase the decidualization level of RIF-hEnSCs. These findings may inspire new ideas about the treatment of RIF.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 6","pages":"397-405"},"PeriodicalIF":2.5,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10003207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selecting the optimal strategies when using nurse sows for supernumerous piglets 超群仔猪使用护理母猪的最佳策略选择

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-05-21 DOI: 10.1002/mrd.23688

Thomas Sønderby Bruun, Trine Friis Pedersen, Flemming Thorup, Anja Varmløse Strathe

{"title":"Selecting the optimal strategies when using nurse sows for supernumerous piglets","authors":"Thomas Sønderby Bruun, Trine Friis Pedersen, Flemming Thorup, Anja Varmløse Strathe","doi":"10.1002/mrd.23688","DOIUrl":"10.1002/mrd.23688","url":null,"abstract":"Hyper-prolific sows frequently do not have a sufficient number of functional teats for their piglets to nurse which has led to the use of nurse sows to manage these surplus piglets. This review discusses strategies for using nurse sows and factors that influence preweaning survival and weight gain of their litters, as well as those that affect their subsequent rebreeding performance. Rearing piglets using a nurse sow can be as successful as piglets reared with their biological mother and is thus a powerful management tool to decrease preweaning piglet mortality. Selecting a young sow as nurse sow is beneficial for piglet survival; however, piglets nursing first parity sows often have a lower daily weight gain than piglets nursing multiparous sows. A litter of uniform surplus piglets is preferably handled using the two-step nurse sow strategy. A consequence of nonuniform litters will most likely be an increased mortality and decreased weaning weight among the smallest piglets within a litter. The subsequent fertility of nurse sows is not compromised. There is an increased risk of lactational oestrus when using nurse sows leading to an increased weaning-to-oestrus interval; however, litter size in nurse sows is identical or even moderately higher in the subsequent parity compared with nonnurse sows.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 7","pages":"546-560"},"PeriodicalIF":2.5,"publicationDate":"2023-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23688","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10232433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1