Molecular Reproduction and Development最新文献

Direct Development of Golf Ball Sponges, Genus Craniella (Demospongiae, Tetractinellida) From the Northeast Atlantic Ocean 东北大西洋高尔夫球海绵属的直接发育（Demospongiae, tetrtinellia）。

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-10-09 DOI: 10.1002/mrd.70059

Paco Cárdenas, Sandrine Chenesseau, Jim Drewery, Alexander Ereskovsky

{"title":"Direct Development of Golf Ball Sponges, Genus Craniella (Demospongiae, Tetractinellida) From the Northeast Atlantic Ocean","authors":"Paco Cárdenas, Sandrine Chenesseau, Jim Drewery, Alexander Ereskovsky","doi":"10.1002/mrd.70059","DOIUrl":"10.1002/mrd.70059","url":null,"abstract":"Among the eight types of development in sponges, the least common and least studied is direct, non-larval development during viviparity. To supplement our knowledge of this rare type of demosponge development, we present here a description of the embryonic development of four species of the genus Craniella (Demospongiae, order Tetractinellida) from the deep-sea in the Northeast Atlantic. Craniella development is asynchronous within one sponge. Mature oocytes are polylecithal and isolecithal. Embryonic development occurs in a dense double-layer follicle: layers of flattened cells and a thick layer of collagen. The cleavage is total, unequal, and asynchronous. It is characterized by collagen layers penetrating inside the embryo and surrounding blastomeres. As a result of cleavage, an oval-shaped apolar stereoblastula is formed. At the stereoblastula stage, embryonic sclerocytes secrete the first megascleres, long thin oxeas, radially positioned. Later, the embryo is divided into the peripheral, intermediate, and central zones. In the intermediate zone, choanocyte chambers, lacunes, and canals of the aquiferous system are formed. The fully formed juveniles have a subspherical to flattened shape with cone-shaped outgrowths on the surface. Unlike the adults, juveniles lack cortical microxeas and have characteristic anamonaenes spicules. The juveniles exit the mother's body through the exhalant canals of the aquiferous system.\u0000 Trial Registration: Not applicable","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 10","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.70059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Aging on Mammalian Spermatogenesis From a Cellular Perspective 从细胞角度看衰老对哺乳动物精子发生的影响。

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-10-07 DOI: 10.1002/mrd.70061

Tao Li, Wenchao Xu, Lisha Yin, Jin Zhang, Opeyemi DhikhirullahI, Zhibing Zhang, Shuiqiao Yuan

引用次数: 0

Post-Embryonic Development and Formation of the Heterocoelic Aquiferous System in Two Species of Calcareous Sponges (Calcarea, Porifera) 两种钙质海绵（calcalarea, Porifera）异腔含水系统的胚胎后发育和形成

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-10-03 DOI: 10.1002/mrd.70060

Emilio Lanna, Michelle Klautau

{"title":"Post-Embryonic Development and Formation of the Heterocoelic Aquiferous System in Two Species of Calcareous Sponges (Calcarea, Porifera)","authors":"Emilio Lanna, Michelle Klautau","doi":"10.1002/mrd.70060","DOIUrl":"https://doi.org/10.1002/mrd.70060","url":null,"abstract":"We characterized the morphogenetic processes of larval metamorphosis and the development of the olynthus and heterocoelic aquiferous system (AS) in Sycettusa hastifera (syconoid) and Paraleucilla magna (leuconoid) (Porifera, Calcarea). Metamorphosis and development up to the olynthus were similar in both species. During metamorphosis, apparently, the juvenile's cell lineages were established by invagination of the ciliated pole into the larval cavity, while posterior pole cells covered the juvenile. Ciliated cells seemed to differentiate into an inner cell mass (ICM) (forming choanoblasts and scleroblasts), while granular cells seemed to form mainly the pinacoderm. Then, cavitation of the ICM formed the asconoid AS of the olynthus. Choanocyte chamber morphogenesis for the syconoid and leuconoid AS seemed to be epithelial, but followed different paths. In S. hastifera, the chambers were formed by folding the primordial choanoderm towards the external portion of the sponge, whereas in P. magna, invagination of the choanoderm into the spongocoel segregated groups of choanocytes into spherical chambers. The morphogenesis of the heterocoelic AS in these calcareans is likely different from that in Demospongiae (leuconoid AS is established via mesenchymal-to-epithelial morphogenesis) but similar to Homoscleromorpha (AS arises through epithelial morphogenesis). Characterizing the post-embryonic development in these species is the first step toward understanding the mechanisms that regulate the ontogeny and evolution of Porifera's primary synapomorphy: the aquiferous system.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 10","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145204774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visfatin Modulates Angiogenesis and Apoptosis in Porcine Luteal Cells via INSR, MAPK, and AKT Pathways Visfatin通过INSR、MAPK和AKT通路调控猪黄体细胞的血管生成和凋亡

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-09-28 DOI: 10.1002/mrd.70058

Ewa Mlyczyńska, Ewa Zaobidna, Edyta Rytelewska, Monika Dawid, Grzegorz Kopij, Marta Kieżun, Kamil Dobrzyń, Nina Smolińska, Tadeusz Kamiński, Agnieszka Rak

{"title":"Visfatin Modulates Angiogenesis and Apoptosis in Porcine Luteal Cells via INSR, MAPK, and AKT Pathways","authors":"Ewa Mlyczyńska, Ewa Zaobidna, Edyta Rytelewska, Monika Dawid, Grzegorz Kopij, Marta Kieżun, Kamil Dobrzyń, Nina Smolińska, Tadeusz Kamiński, Agnieszka Rak","doi":"10.1002/mrd.70058","DOIUrl":"10.1002/mrd.70058","url":null,"abstract":"<div>\u0000 \u0000 Visfatin, a multifunctional adipokine, plays a crucial role in ovarian physiology. This study examines its effects on the level of selected angiogenesis and apoptosis factors in the porcine corpus luteum (CL). Using in vitro cultures of luteal cells, we analyzed the effects of visfatin on key angiogenic factors (VEGF family, PDGF, bFGF2, ANG, and iNOS) and their receptors. Additionally, we assessed luteal cell viability and apoptosis by measuring caspase activity, DNA fragmentation, and transcript levels of apoptotic factors. We noted that visfatin mostly decreases the expression of angiogenic factors and their receptors during the luteal phase except for VEGFC, VEGFR3 on Days 2–3 of the estrous cycle, and VEGFB, VEGFD, VEGFR1, VEGFR2, FGFR2 on Days 14–16 of the estrous cycle, which were upregulated. Moreover, visfatin inhibits apoptosis by downregulating caspase-3, -8, -9, BAX, and caspase 3/7 activity while increasing BCL-2 and luteal cells viability. Treatment with FK866, nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, largely disrupted observed effects, highlighting the NAMPT-dependent function of visfatin. Moreover, visfatin suppressed VEGF-A levels via insulin receptor and MAPK, while insulin receptor, MAPK, and AKT pathways mediated the inhibition of caspase 3/7 activity. These findings suggest that visfatin regulates apoptosis and angiogenesis in the porcine CL.\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Genes Involved in the Circadian Clock With Polysomes in the Bovine Preimplantation Embryo 牛着床前胚胎中参与生物钟的基因与多体的关联。

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-09-25 DOI: 10.1002/mrd.70054

Daniel L. Stanton, Linkai Zhu, Zongliang Jiang, Peter J. Hansen

{"title":"Association of Genes Involved in the Circadian Clock With Polysomes in the Bovine Preimplantation Embryo","authors":"Daniel L. Stanton, Linkai Zhu, Zongliang Jiang, Peter J. Hansen","doi":"10.1002/mrd.70054","DOIUrl":"10.1002/mrd.70054","url":null,"abstract":"<div>\u0000 \u0000 The circadian clock is not functional during preimplantation development because lack of transcription prevents the feedback loop required for circadian cyclicity. Moreover, transcript abundance for the core clock genes (CLOCK, ARNTL, PER1, PER2, CRY1, and CRY2) declines in the embryo after embryonic genome activation. Nonetheless, transcripts for each of the clock genes are present in the embryo. The potential for translation of these transcripts in the bovine embryo was evaluated by assessing whether circadian clock genes are associated with polysomes and whether these transcripts are preferentially retained as development proceeds to the blastocyst stage. Transcript abundance declined at the eight-cell stage for BMAL1 and CRY1 and at the morula stage for PER1, PER2, and CRY2. Before embryonic genome activation at the eight-cell stage, a large fraction of transcripts for each of the genes was associated with polysomes. At specific later stages of development, there was less transcript associated with polysomes than with other fractions. This was true for PER1 at the morula and blastocyst stage, PER2 at the morula stage, and CRY1 at the eight-cell stage. The percent of transcripts associated with polysomes was also calculated. This value was lower after the two-cell stage for CLOCK and PER1. Based on the decrease in transcript abundance and proportional association with polysomes after the two-cell stage, it was concluded that capacity for translation of circadian clock genes declines in the preimplantation embryo as development proceeds. Thus, de novo synthesized proteins involved in the circadian clock mechanism are unlikely to play an important function in the preimplantation embryo following embryonic genome activation.\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Preliminary Study of circ-PNPT1/miR-144-3p/UBE2G1 Expression in Placental Tissues and Serum and Its Mechanism in Patients With Gestational Diabetes Mellitus 妊娠期糖尿病患者胎盘组织和血清中circ-PNPT1/miR-144-3p/UBE2G1表达及其机制的初步研究

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-09-25 DOI: 10.1002/mrd.70057

Jingyi Wang, Minire Tuluhong, Qiying Zhu

{"title":"A Preliminary Study of circ-PNPT1/miR-144-3p/UBE2G1 Expression in Placental Tissues and Serum and Its Mechanism in Patients With Gestational Diabetes Mellitus","authors":"Jingyi Wang, Minire Tuluhong, Qiying Zhu","doi":"10.1002/mrd.70057","DOIUrl":"10.1002/mrd.70057","url":null,"abstract":"<div>\u0000 \u0000 Circular RNAs are associated with important pathophysiological characteristics of gestational diabetes mellitus (GDM). This study preliminarily explored circular polyribonucleotide nucleotidyltransferase 1 (circ-PNPT1) expression in placental tissues and serum of GDM patients and its role in regulating the miR-144-3p/UBE2G1 axis to affect endothelial dysfunction in GDM. Twenty GDM patients and 20 pregnant women with normal glucose tolerance were enrolled. Human umbilical vein endothelial cells (HUVECs) were cultured with high glucose (HG) to establish an in vitro GDM model, then treated with short hairpin-circ-PNPT1 and a lentiviral empty plasmid. Circ-PNPT1 levels, HUVEC viability, endothelial function, and oxidative damage were assessed using RT-qPCR tube formation, CCK-8, flow cytometry, and Transwell assays, respectively. Normally cultured and HG-cultured HUVEC samples underwent small RNA sequencing to analyze differentially expressed miRNAs. The possible miRNAs and mRNAs downstream of circ-PNPT1 were screened using bioinformatics and verified using RT-qPCR, dual luciferase reporter assay, and Ago2-RIP. GDM patients exhibited highly expressed circ-PNPT1 and UBE2G1, and weakly expressed miR-144-3p in placental tissues and serum. In vitro, HG-treated HUVECs displayed highly expressed circ-PNPT1 and cellular dysfunction, as evidenced by reduced cell survival, enhanced apoptosis, decreased cell migration and angiogenesis, an elevated MDA level, and downregulated SOD and GSH-Px levels. Circ-PNPT1 knockdown alleviated HG-induced HUVEC dysfunction. circ-PNPT1 might target and bind miR-144-3p; miR-144-3p might target and bind UBE2G1. Additionally, circ-PNPT1 might act as a competing endogenous RNA for miR-144-3p to regulate UBE2G1 expression in HG-induced HUVECs. Taken together, circ-PNPT1 modulates HG-induced HUVEC dysfunction via the miR-144-3p/UBE2G1 axis.\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time and Media-Dependent Modulation of Sperm Capacitation in Red-Rumped Agouti (Dasyprocta leporina Linnaeus, 1758) 红臀刺鼠精子获能的时间和媒介依赖性调节（Dasyprocta leporina Linnaeus, 1758）

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-09-02 DOI: 10.1002/mrd.70056

Lhara Ricarliany Medeiros de Oliveira, Leonardo Vitorino Costa de Aquino, Antonia Beatriz Mendonça Pereira, Ana Lívia Rocha Rodrigues, Luanna Lorenna Vieira Rodrigues, Luana Grasiele Pereira Bezerra, Romário Parente dos Santos, Moacir Franco de Oliveira, Alexandre Rodrigues Silva, Alexsandra Fernandes Pereira

{"title":"Time and Media-Dependent Modulation of Sperm Capacitation in Red-Rumped Agouti (Dasyprocta leporina Linnaeus, 1758)","authors":"Lhara Ricarliany Medeiros de Oliveira, Leonardo Vitorino Costa de Aquino, Antonia Beatriz Mendonça Pereira, Ana Lívia Rocha Rodrigues, Luanna Lorenna Vieira Rodrigues, Luana Grasiele Pereira Bezerra, Romário Parente dos Santos, Moacir Franco de Oliveira, Alexandre Rodrigues Silva, Alexsandra Fernandes Pereira","doi":"10.1002/mrd.70056","DOIUrl":"https://doi.org/10.1002/mrd.70056","url":null,"abstract":"The increasing focus on understanding spermatozoa mechanisms in rodents aims to enhance reproductive stability and support conservation efforts, particularly for ecologically significant and declining species like the red-rumped agouti. We aimed to evaluate the interaction between capacitation media and time, testing BSA concentrations—low (4 mg/mL) and high (15 mg/mL)—with or without 2 mM calcium chloride (CaCl2) across three time points. Epididymal sperm were distributed into five groups: without capacitation agent (WCA), high BSA (HBSA), high BSA with CaCl2 (HBCa), low BSA (LBSA), and low BSA with CaCl2 (LBCa), each incubated for 1, 3, or 6 h. Total sperm motility was significantly higher only in media with CaCl2. However, only LBCa maintained high progressive motility. LBCa and HBCa maintained membrane integrity, mitochondrial functionality, and reduced reactive oxygen species levels, but only LBCa increased glutathione levels. HBSA, HBCa, LBSA, and LBCa improved the sperm capacitation rate, but LBCa yielded the highest proportion of capacitated sperm and acrosome-reacted cells after 6 h. Hyperactivation rates were higher in both LBCa and HBCa after 6 h. Therefore, the optimal condition for red-rumped agouti sperm capacitation is a low BSA concentration (4 mg/mL) supplemented with 2 mM calcium chloride following a 6 h incubation.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful Generation of Germline Tyrosinase Gene Edited Thirteen-Lined Ground Squirrels (Ictidomys tridecemlineatus Mitchill 1821) 成功编辑了种系酪氨酸酶基因的十三棱地松鼠（Ictidomys tridecemlineatus Mitchill 1821）

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-09-02 DOI: 10.1002/mrd.70055

Akiko Takizawa, Jamie Foeckler, Emily Knapp, Michael Grzybowski, Aron M. Geurts, Joseph Carroll, Dana K. Merriman

引用次数: 0

Characterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes 未成熟和体外成熟猫卵母细胞脂质特征的研究

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-08-27 DOI: 10.1002/mrd.70052

Erlandia M. Vasconcelos, Thais G. de Oliveira, Ribrio I. T. P. Batista, Paulo S. C. Rangel, Georgia C. Atella, Gabriela R. Leal, Joanna M. G. Souza-Fabjan

{"title":"Characterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes","authors":"Erlandia M. Vasconcelos, Thais G. de Oliveira, Ribrio I. T. P. Batista, Paulo S. C. Rangel, Georgia C. Atella, Gabriela R. Leal, Joanna M. G. Souza-Fabjan","doi":"10.1002/mrd.70052","DOIUrl":"https://doi.org/10.1002/mrd.70052","url":null,"abstract":"Forskolin (FSK), l-carnitine (LC), and Conjugated Linoleic Acid (CLA) are lipid modulators that reduce cellular lipid content. This study characterizes the lipid profile and mRNA content of immature and in vitro matured cat oocytes with or without a MIX of these modulators. Ovaries were collected, COC retrieved, and allocated into three groups: immature (IM), matured without (CONT), or with FSK, LC, and CLA (MIX). Lipid analysis was performed using High-performance thin-layer chromatography, gene expression by RT-qPCR, and oocytes stained with Nile Red, Oil Red O, and Sudan Black B. Eight lipid subclasses were detected in all groups. Esterified sterol was more abundant in CONT than IM, with intermediate levels in MIX. Triacylglycerol was higher in MIX than IM, while monoacylglycerol increased in both CONT and MIX. In MIX, DGAT1, FABP3, and PLIN2 were upregulated compared to IM. Moreover, in IM, DGAT1 was downregulated and FABP3 upregulated relative to CONT. FABP3 levels were higher in MIX than CONT. Oil Red O and Sudan Black B staining showed reduced lipid content in MIX compared to CONT, while Nile Red detected no difference. In conclusion, feline oocytes subjected to MIX during in vitro maturation exhibited increased triacylglycerol levels and enhanced FABP3 expression.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144910402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicles Derived From Endometrial Stem Cells Preconditioned With PGE2-Reverse Myofibroblast Phenotype in Mare Endometrial Cells: A Novel Anti-Fibrotic Approach 子宫内膜干细胞经pge2逆转肌成纤维细胞表型预处理后的细胞外囊泡：一种新的抗纤维化方法

IF 3 3区生物学

Molecular Reproduction and Development Pub Date : 2025-08-27 DOI: 10.1002/mrd.70053

Lidice Méndez-Pérez, Belén O. Ibáñez, Sebastián Rodríguez, Yat Sen Wong, Diego Caamaño, Felipe I. Navarrete, Joel Cabezas, Ana C. Mançanares, Carlos Escudero, Lleretny Rodríguez-Álvarez, Fidel Ovidio Castro

{"title":"Extracellular Vesicles Derived From Endometrial Stem Cells Preconditioned With PGE2-Reverse Myofibroblast Phenotype in Mare Endometrial Cells: A Novel Anti-Fibrotic Approach","authors":"Lidice Méndez-Pérez, Belén O. Ibáñez, Sebastián Rodríguez, Yat Sen Wong, Diego Caamaño, Felipe I. Navarrete, Joel Cabezas, Ana C. Mançanares, Carlos Escudero, Lleretny Rodríguez-Álvarez, Fidel Ovidio Castro","doi":"10.1002/mrd.70053","DOIUrl":"https://doi.org/10.1002/mrd.70053","url":null,"abstract":"<div>\u0000 \u0000 Endometrial fibrosis in mares compromises fertility through aberrant extracellular matrix deposition and sustained myofibroblast activation. Conventional interventions fail to reverse these pathological alterations, necessitating innovative, mechanism-focused therapies. In this study, we pioneered the use of prostaglandin E2 (PGE2) preconditioning of equine endometrial-derived mesenchymal stem cells (ET-eMSCs) and their extracellular vesicles (EVs) to target fibrotic processes directly. ET-eMSCs were isolated from mare endometrial biopsies pretreated with PGE2 to enhance their anti-fibrotic secretome, and EVs were subsequently harvested. In vitro assays demonstrated that PGE2-preconditioned ET-eMSCs and their EVs inhibited α-smooth muscle actin expression, reduced collagen I deposition, and restored key endometrial markers of receptivity and proliferation. These findings establish the first evidence that PGE2-enhanced ET-eMSC-derived EVs can exert anti-fibrotic effects in an in vitro model of equine endometrial fibrosis. This study provides a robust translational framework for developing targeted regenerative therapies for fibrotic diseases across species, with potential applications in human reproductive medicine.\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144910403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0