Molecular Reproduction and Development最新文献

{"title":"A pilot study on the relationship between thrombospondin-1 (THBS1) and transforming growth factor beta1 (TGFβ1) in the bovine placenta during early mid-pregnancy as well as parturition with normally released and retained placenta","authors":"Jacek Wawrzykowski,&nbsp;Monika Jamioł,&nbsp;Marta Kankofer","doi":"10.1002/mrd.23710","DOIUrl":"10.1002/mrd.23710","url":null,"abstract":"<p>During pregnancy, it is necessary to create appropriate conditions for the development of the placenta and the fetus. However, during parturition, the placenta must be separated and subsequently removed as soon as possible to not expose the female to the possibility of infection. In this study, the relationship between thrombospondin-1 (THBS1) and transforming growth factor beta1 (TGFβ1) concentrations was described during bovine pregnancy (second, fourth, and sixth months; <i>n</i> = 3/each month), at normal parturition (NR) and parturition with fetal membrane retention (R). The presence of THBS1 and TGFβ1 was confirmed in bovine placental tissues of both maternal and fetal parts. Enzyme-linked immunosorbent assay showed statistically significant differences (<i>p</i> &lt; 0.05) in THBS1 concentrations (pg/mg protein) between examined parturient samples (maternal part: 5.76 ± 1.61 in R vs. 2.26 ± 1.58 in NR; fetal part: 2.62 ± 1.94 in R vs. 1.70 ± 0.23 in NR). TGFβ1 concentrations (pg/mg protein) were significantly lower (<i>p</i> &lt; 0.05) in the retained fetal membranes compared to the released fetal membranes in the maternal part of the placenta (26.22 ± 7.53 in NR vs. 17.80 ± 5.01 in <i>R</i>). The participation of THBS1 in the activation of TGFβ1 in parturient bovine placental tissues leading to the normal release of fetal membranes may be suggested.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41154467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A strong foundation for the next generation","authors":"Bettina P. Mihalas,&nbsp;Emily R. Frost,&nbsp;Kaushiki M. Kadam,&nbsp;Irene E. Sucquart,&nbsp;Prakruti Sirigeri,&nbsp;William L. Ledger,&nbsp;Robert B. Gilchrist","doi":"10.1002/mrd.23709","DOIUrl":"10.1002/mrd.23709","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 10-11","pages":"773"},"PeriodicalIF":2.5,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HDAC4 regulates the proliferation, migration, and invasion of trophoblasts in pre-eclampsia through the miR-134-5p/FOXM1 axis","authors":"Yanli Xu,&nbsp;Xiaodi Kang,&nbsp;Hongli Jiang,&nbsp;Huafang Liu,&nbsp;Wenjing Wang","doi":"10.1002/mrd.23706","DOIUrl":"10.1002/mrd.23706","url":null,"abstract":"<p>Epigenetics, including histone modifications and noncoding RNAs, affects abnormal placental function in pre-eclampsia (PE). This study was conducted to explore the role of histone deacetylase 4 (HDAC4) in trophoblast invasion and migration. The expression levels of HDAC4, microRNA (miR)-134-5p, and forkhead box protein M1 (FOXM1) in placentas from PE patients and healthy controls and their correlations were examined. HTR8/SVneo cells were cultured and underwent gene intervention. Then, trophoblast proliferation, invasion, and migration were evaluated by 5-ethynyl-2ʹdeoxyuridine, Transwell, and scratch assays. The enrichments of HDAC4 and acetylated histone H3 at lysine 9 (H3K9Ac) on the miR-134-5p promoter were quantified by chromatin immunoprecipitation. The binding of miR-134-5p to FOXM1 was analyzed by dual-luciferase assay. HDAC4 and FOXM1 were downregulated while miR-134-5p was upregulated in PE placentas. HDAC4 downregulation impaired trophoblast proliferation, invasion, and migration while HDAC4 overexpression played the opposite role. Mechanically, HDAC4 deacetylated H3K9Ac to repress miR-134-5p expression by erasing H3K9Ac, reduced the binding of miR-134-5p to FOXM1, and then promoted FOXM1 transcription. miR-134-5p overexpression or FOXM1 downregulation abrogated the promotive role of HDAC overexpression in trophoblast invasion and migration. Our study unraveled a novel mechanism of trophoblast proliferation, invasion, and migration and proposed that HDAC4 may be a promising target for the treatment of PE.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 12","pages":"849-860"},"PeriodicalIF":2.5,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41139126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mevalonate pathway and male reproductive aging","authors":"Jia-Le Zhang,&nbsp;Meng Lv,&nbsp;Chao-Fan Yang,&nbsp;Ying-Xi Zhu,&nbsp;Chao-Jun Li","doi":"10.1002/mrd.23705","DOIUrl":"10.1002/mrd.23705","url":null,"abstract":"<p>Male fertility declines with age. The mevalonate pathway, through which cholesterol and nonsteroidal isoprenoids are synthesized, plays key role in metabolic processes and is an essential pathway for cholesterol production and protein prenylation. Male reproductive aging is accompanied by dramatic changes in the metabolic microenvironment of the testis. Since the mevalonate pathway has an important role in spermatogenesis, we attempted to explore the association between male reproductive aging and the mevalonate pathway to explain the mechanism of male reproductive aging. Alterations in the mevalonate pathway may affect male reproductive aging by decreasing cholesterol synthesis and altering testis protein prenylation. Decreased cholesterol levels affect cholesterol modification, testosterone production, and remodeling of germ cell membranes. Aging-related metabolic disorders also affect the metabolic coupling between somatic cells and spermatogenic cells, leading to male fertility decline. Therefore, we hypothesized that alterations in the mevalonate pathway represent one of the metabolic causes of reproductive aging.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 10-11","pages":"774-781"},"PeriodicalIF":2.5,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41139218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thankyou to our Sponsors","authors":"","doi":"10.1002/mrd.23704","DOIUrl":"https://doi.org/10.1002/mrd.23704","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23704","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50138510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trimethylation profile of histones H3 lysine 4 and 9 in late preantral and early antral caprine follicles grown in vivo versus in vitro in the presence of anethole","authors":"Ana F. B. Silva,&nbsp;Ana N. P. Morais,&nbsp;Laritza F. Lima,&nbsp;Anna C. A. Ferreira,&nbsp;Renato F. Silva,&nbsp;Naiza A. R. Sá,&nbsp;Satish Kumar,&nbsp;Ariclécio C. Oliveira,&nbsp;Benner G. Alves,&nbsp;Ana P. R. Rodrigues,&nbsp;Eduardo L. Gastal,&nbsp;Vilceu Bordignon,&nbsp;José R. Figueiredo","doi":"10.1002/mrd.23700","DOIUrl":"10.1002/mrd.23700","url":null,"abstract":"<p>This study assessed the histones methylation profile (H3K4me3 and H3K9me3) in late preantral (PA) and early antral (EA) caprine follicles grown in vivo and in vitro, and the anethole effect during in vitro culture of PA follicles. Uncultured in vivo–grown follicles (PA, <i>n</i> = 64; EA, <i>n</i> = 73) were used as controls to assess the methylation profile and genes' expression related to apoptosis cascade (<i>BAX</i>, proapoptotic; <i>BCL2</i>, antiapoptotic), steroidogenesis (<i>CYP17</i>, <i>CYP19A1</i>), and demethylation (<i>KDM1AX1</i>, <i>KDM1AX2</i>, <i>KDM3A</i>). The isolated PA follicles (<i>n</i> = 174) were cultured in vitro for 6 days in α-MEM⁺ in either absence (control) or presence of anethole. After culture, EA follicles were evaluated for methylation, mRNA abundance, and morphometry. Follicle diameter increased after culture, regardless of treatment. The methylation profile and the mRNA abundance were similar between in vivo–grown PA and EA follicles. Anethole treatment led to higher H3K4me3 fluorescence intensity in EA follicles. The mRNA abundances of <i>BAX</i>, <i>CYP17</i>, and <i>CYP19A1</i> were higher, and <i>BCL2</i> and <i>KDM3A</i> were lower in in vitro–grown EA follicles than in vivo–grown follicles. In conclusion, in vitro follicle culture affected H3K4me3 fluorescence intensity, mRNA abundance of apoptotic genes, and steroidogenic and demethylase enzymes compared with in vivo–grown follicles.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 12","pages":"810-823"},"PeriodicalIF":2.5,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10163345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Control of pig reproduction XI","authors":"Clay A. Lents,&nbsp;Nicoline M. Soede","doi":"10.1002/mrd.23696","DOIUrl":"10.1002/mrd.23696","url":null,"abstract":"<p></p><p>11th International Conference on Pig Reproduction</p><p>5th – 7th June 2023</p><p>Ghent Belgium</p><p>The 11th International Conference on Pig Reproduction (ICPR) was held in the beautiful city of Ghent, Belgium, on June 5-7 in 2023. Former ICPR meetings had always taken place at 4-year intervals, but the worldwide epidemic of Corona-19 forced us to a 2-year delay, and we are thankful that we could again meet to discuss pig reproduction. The program of the meeting was developed by the International Organizing Committee. The contributions of 26 invited speakers focused on new insights into specific reproductive processes in female and male pigs and their gametes, and on genetic, environmental or management factors influencing these processes. A total of 74 posters were presented, of which 14 were selected to deliver an oral presentation, and 26 gave a flash presentation, which was a first at an ICPR meeting but represents a continued commitment of ICPR to trainee and student development.</p><p>The success of the 11th ICPR was due to the hard work and commitment of the local organising committee, chaired by Dr Dominiek Maes. The conference ran smoothly and was enjoyed by the delegates, providing a forum for stimulating scientific exchange with colleagues old and new. The conference attracted over 130 delegates from at least 18 countries, and was funded, in part, by the USDA National Institute of Food and Agriculture (Agriculture and Food Research Initiative Competitive Grant no. 2021-67015-33405). An outstanding meeting was further made possible by many industry and academic sponsors. The detailed program can be found at https://icpr2023.be.</p><p>Proceedings of previous ICPR meetings 1 through 9 can be found at www.biosciproceedings.org. Proceedings of the 10th ICPR were published as a special issue of Molecular Reproduction and Development (MRD) and are available at https://onlinelibrary.wiley.com/toc/10982795/2017/84/9. The ICPR is happy to again partner with MRD for the current proceedings entitled <i>Control of Pig Reproduction XI</i>. It contains review articles accompanying the invited lectures that have been internationally peer-reviewed, and abstracts of the posters and oral presentations that were peer-reviewed by the local and international organizing committees. Sincere thanks are due to the many external scientific reviewers, MRD editorial board members, and ICPR co-editors Inma Parrilla and Agnieszka Waclawik. The ICPR thanks Harvey Florman, Eunice Precious Jasmine D and Ekta Kumar at MRD for their hard work and commitment. We are confident that this Special Issue will provide a valuable resource for everyone interested in pig reproduction and fertility.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 7","pages":"419"},"PeriodicalIF":2.5,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23696","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10126603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICPR ABSTRACTS","authors":"","doi":"10.1002/mrd.23697","DOIUrl":"https://doi.org/10.1002/mrd.23697","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 7","pages":"708-745"},"PeriodicalIF":2.5,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50156007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic evolution of the postpartum dairy cow uterus","authors":"Nicolas Aranciaga,&nbsp;Alastair B. Ross,&nbsp;James D. Morton,&nbsp;Robin McDonald,&nbsp;Jessica L. Gathercole,&nbsp;Debra K. Berg","doi":"10.1002/mrd.23702","DOIUrl":"10.1002/mrd.23702","url":null,"abstract":"<p>High rates of early pregnancy loss are a critical issue in dairy herds, particularly in seasonal, grazing systems. Components of the uterine luminal fluid (ULF), on which the early embryo depends for sustenance and growth, partly determine early pregnancy losses. Here, changes in ULF from early to mid-postpartum in crossbred dairy cows were explored, linking them with divergent embryo development. For this, the uteri of 87 cows at Day 7 of pregnancy at first and third estrus postpartum were flushed to collect ULF. Eighteen metabolites (chiefly organic acids and sugars) significantly varied in abundance across postpartum, indicating a molecular signature of physiological recovery consistent of the upregulation of pyrimidine metabolism and glycerophospholipid metabolism, and downregulation of pentose phosphate and taurine metabolism pathways. Joint pathway analysis of metabolomics data and a previously generated proteomics data set on the same ULF samples suggests key links between postpartum recovery and subsequent successful embryo development. These include upregulation of VEGFA and downregulation of metabolism, NRF2, T-cell receptor, which appear to improve the ULF's capacity of sustaining normal embryo development, and a putative osmo-protectant role of beta-alanine. These relationships should be further investigated to develop tools to detect and reduce early pregnancy loss in dairy cows.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 12","pages":"835-848"},"PeriodicalIF":2.5,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10430595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Participation of apoptotic markers in the process of maturation and elimination of spermatozoa in the epididymis of the Corynorhinus mexicanus bat","authors":"Gihovani Ademir Samano-Barbosa,&nbsp;Blanca Patrícia López-Trinidad,&nbsp;Ahiezer Rodríguez-Tobón,&nbsp;Julio Cesar Chávez-Zamora,&nbsp;Edith Cortés-Barberena,&nbsp;Miguel Angel León-Galván,&nbsp;Ernesto Rodríguez-Tobón,&nbsp;Edith Arenas-Ríos","doi":"10.1002/mrd.23701","DOIUrl":"10.1002/mrd.23701","url":null,"abstract":"<p>The <i>Corynorhinus mexicanus</i> bat is characterized by a specific form of reproductive asynchrony between males and females. After mating, some sperm remain in the male's epididymis, the organ where the sperm had matured. It has not yet been determined if apoptotic markers participate in the process of the maturation and/or elimination of these cells, so studying this topic is essential for our understanding of this species. Male bats were collected during three stages: Before mating; during the Mating phase; After mating and the final phase, which we call, Storage. Their epididymides were removed, weighed and measured. Sperm were extracted and the following sperm parameters were evaluated: active caspases, phosphatidylserine externalization, and mitochondrial membrane potential. Sperm from the testes enter the epididymis during Before mating, causing the organ to grow. During Mating phase, spermatozoa present a large amount of active caspases with externalization of phosphatidyl serine, even while still alive. This suggests that these two markers could participate in maturation and elimination, respectively.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"90 8-9","pages":"749-757"},"PeriodicalIF":2.5,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23701","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9975197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}