Molecular Reproduction and Development最新文献_第5页

hsd17b1 is a key gene for ovarian differentiation of the Siberian sturgeon hsd17b1 是西伯利亚鲟卵巢分化的关键基因

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2024-01-25 DOI: 10.1002/mrd.23729

André Lasalle, Germán Benech-Correa, Frédéric G. Brunet, Denise Vizziano-Cantonnet

{"title":"hsd17b1 is a key gene for ovarian differentiation of the Siberian sturgeon","authors":"André Lasalle, Germán Benech-Correa, Frédéric G. Brunet, Denise Vizziano-Cantonnet","doi":"10.1002/mrd.23729","DOIUrl":"10.1002/mrd.23729","url":null,"abstract":"This is the first work using gonads from undifferentiated, genetically-sexed Siberian sturgeon describing expression changes in genes related to steroid synthesis and female and male sex differentiation. One factor identified as relevant for ovarian differentiation was the gene coding for the enzyme Hsd17b1, which converts estrone into estradiol-17β. hsd17b1 was highly activated in female gonads at 2.5 months of age, around the onset of sex differentiation, preceding activation of two other genes involved in estrogen production (cyp19a1 and foxl2). hsd17b1 was also strongly repressed in males. Two known foxl2 paralogs are found in Siberian sturgeon—foxl2 and foxl2l—but only foxl2 appeared to be associated with ovarian differentiation. With regard to the male pathway, neither 11-oxygenated androgens nor classic male genes (amh, dmrt1, sox9, and dhh) were found to be involved in male sex differentiation, leaving open the question of which genes participate in early male gonad development in this ancient fish. Taken together, these results indicate an estrogen-dependence of female sex differentiation and 11-oxygenated androgen-independence of male sex differentiation.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139558331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of seminal fluid production after mating 交配后精液产生的动态变化

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2024-01-25 DOI: 10.1002/mrd.23732

Yvonne Kortsmit, Janine Mariën, Joris M. Koene, Yumi Nakadera

{"title":"Dynamics of seminal fluid production after mating","authors":"Yvonne Kortsmit, Janine Mariën, Joris M. Koene, Yumi Nakadera","doi":"10.1002/mrd.23732","DOIUrl":"10.1002/mrd.23732","url":null,"abstract":"Seminal fluid proteins (SFPs) play vital roles for optimizing reproductive success in diverse animals. Underlining their significance, SFP production and transfer are highly plastic, e.g., depending on the presence of rivals or mating status of partners. However, surprisingly little is known about replenishing SFPs after mating. This is especially relevant in species that mate multiple times, as they continuously produce and use SFPs throughout their reproductive life. Here we examined the expression pattern of SFP genes after mating in the great pond snail, Lymnaea stagnalis. Our results show that two out of the six SFP genes investigated here were upregulated 1 week after mating. Surprisingly, most SFP genes did not change their expression immediately after mating. Even after 1 week, when supposedly seminal fluid is fully replenished, the expression of SFP genes is rather high. In addition, the difference with previous studies hints at the possibility that SFP production after mating is plastic and depends on the mating history of female-acting snails. Our results shed light on unexplored aspects of SFP production, thereby expanding the understanding of reproductive strategies in animals.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.23732","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139558146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Submicron-scale chromatin architecture in Cycas revoluta pollen nuclei 苏铁花粉核的亚微米级染色质结构

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2024-01-24 DOI: 10.1002/mrd.23726

Yukiho Toyama, Tetsuya Higashiyama

引用次数: 0

Deletion of the Sodium Glucose Cotransporter 1 (Sglt-1) impairs mouse sperm movement 缺失葡萄糖钠共转运体 1 (Sglt-1) 会影响小鼠精子的运动

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2024-01-23 DOI: 10.1002/mrd.23723

September Numata, Mumtarin Jannat Oishee, Jeffrey McDermott, Hermann Koepsell, Volker Vallon, Gustavo Blanco

{"title":"Deletion of the Sodium Glucose Cotransporter 1 (Sglt-1) impairs mouse sperm movement","authors":"September Numata, Mumtarin Jannat Oishee, Jeffrey McDermott, Hermann Koepsell, Volker Vallon, Gustavo Blanco","doi":"10.1002/mrd.23723","DOIUrl":"https://doi.org/10.1002/mrd.23723","url":null,"abstract":"The Sodium Glucose Cotransporter Isoform 1 (Sglt-1) is a symporter that moves Na+ and glucose into the cell. While most studies have focused on the role of Sglt-1 in the small intestine and kidney, little is known about this transporter's expression and function in other tissues. We have previously shown that Sglt-1 is expressed in the mouse sperm flagellum and that its inhibition interferes with sperm metabolism and function. Here, we further investigated the importance of Sglt-1 in sperm, using a Sglt-1 knockout mouse (Sglt-1 KO). RNA, immunocytochemistry, and glucose uptake analysis confirmed the ablation of Sglt-1 in sperm. Sglt-1 KO male mice are fertile and exhibit normal sperm counts and morphology. However, Sglt-1 null sperm displayed a significant reduction in total, progressive and other parameters of sperm motility compared to wild type (WT) sperm. The reduction in motility was exacerbated when sperm were challenged to swim in media with higher viscosity. Parameters of capacitation, namely protein tyrosine phosphorylation and acrosomal reaction, were similar in Sglt-1 KO and WT sperm. However, Sglt-1 KO sperm displayed a significant decrease in hyperactivation. The impaired motility of Sglt-1 null sperm was observed in media containing glucose as the only energy substrate. Interestingly, the addition of pyruvate and lactate to the media partially recovered sperm motility of Sglt-1 KO sperm, both in the low and high viscosity media. Altogether, these results support an important role for Sglt-1 in sperm energetics and function, providing sperm with a higher capacity for glucose uptake.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139550227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The targeting of DAPK1 with miR-190a-3p promotes autophagy in trophoblast cells 用 miR-190a-3p 靶向 DAPK1 可促进滋养层细胞的自噬作用

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2024-01-23 DOI: 10.1002/mrd.23724

Qi-qi Luo, Yu Tian, Guang-jin Qu, Kun Huang, Pan-pan Hu, Ying Xue, Bi-feng Hu, Shan-shun Luo

{"title":"The targeting of DAPK1 with miR-190a-3p promotes autophagy in trophoblast cells","authors":"Qi-qi Luo, Yu Tian, Guang-jin Qu, Kun Huang, Pan-pan Hu, Ying Xue, Bi-feng Hu, Shan-shun Luo","doi":"10.1002/mrd.23724","DOIUrl":"https://doi.org/10.1002/mrd.23724","url":null,"abstract":"Pre-eclampsia (PE) is a dangerous pathological status that occurs during pregnancy and is a leading reason for both maternal and fetal death. Autophagy is necessary for cellular survival in the face of environmental stress as well as cellular homeostasis and energy management. Aberrant microRNA (miRNA) expression is crucial in the pathophysiology of PE. Although studies have shown that miRNA (miR)-190a-3p function is tissue-specific, the precise involvement of miR-190a-3p in PE has yet to be determined. We discovered that miR-190a-3p was significantly lower and death-associated protein kinase 1 (DAPK1) was significantly higher in PE placental tissues compared to normal tissues, which is consistent with the results in cells. The luciferase analyses demonstrated the target-regulatory relationship between miR-190a-3p and DAPK1. The inhibitory effect of miR-190a-3p on autophagy was reversed by co-transfection of si-DAPK1 and miR-190a-3p inhibitors. Thus, our data indicate that the hypoxia-dependent miR-190a-3p/DAPK1 regulatory pathway is implicated in the development and progression of PE by promoting autophagy in trophoblast cells.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139550228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long noncoding RNAs in human reproductive processes and diseases 人类生殖过程和疾病中的长非编码 RNA

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2024-01-18 DOI: 10.1002/mrd.23728

Le Zhang, Hailong Sun, Xiujuan Chen

引用次数: 0

Preimplantation genetic testing: A remarkable history of pioneering, technical challenges, innovations, and ethical considerations 植入前基因检测：一部关于开拓、技术挑战、创新和伦理考量的非凡历史

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2024-01-18 DOI: 10.1002/mrd.23727

Keith E. Latham

引用次数: 0

Germ plasm dynamics during oogenesis and early embryonic development in Xenopus and zebrafish 爪蟾和斑马鱼卵子发生和早期胚胎发育过程中的胚盘动力学

IF 2.7 3区生物学

Molecular Reproduction and Development Pub Date : 2023-12-21 DOI: 10.1002/mrd.23718

Divyanshi, Jing Yang

引用次数: 0

Table of Contents, Volume 90, Issue 12, December 2023 目录，第 90 卷第 12 期，2023 年 12 月

IF 2.5 3区生物学

Molecular Reproduction and Development Pub Date : 2023-12-19 DOI: 10.1002/mrd.23613

引用次数: 0

Significant alteration of protein profiles in a mouse model of polycystic ovary syndrome 多囊卵巢综合征小鼠模型的蛋白质谱发生了显著变化。

IF 2.7 3区生物学

Molecular Reproduction and Development Pub Date : 2023-12-06 DOI: 10.1002/mrd.23720

Bin Meng, Xiaoning Yang, Shiwei Luo, Chong Shen, Jia Qi, Haifeng Zhang, Yandong Li, Ying Xue, Juan Zhao, Pengxiang Qu, Enqi Liu

{"title":"Significant alteration of protein profiles in a mouse model of polycystic ovary syndrome","authors":"Bin Meng, Xiaoning Yang, Shiwei Luo, Chong Shen, Jia Qi, Haifeng Zhang, Yandong Li, Ying Xue, Juan Zhao, Pengxiang Qu, Enqi Liu","doi":"10.1002/mrd.23720","DOIUrl":"10.1002/mrd.23720","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is an endocrine disorder, affecting women of child-bearing age, and the incidence rate is growing and assuming epidemic proportions. The etiology of PCOS remains unknown and there is no cure. Some animal models for PCOS have been established which have enhanced our understanding of the underlying mechanisms, but omics data for revealing PCOS pathogenesis and for drug discovery are still lacking. In the present study, proteomics analysis was used to construct a protein profile of the ovaries in a PCOS mouse model. The result showed a clear difference in protein profile between the PCOS and control group, with 495 upregulated proteins and 404 downregulated proteins in the PCOS group. The GO term and KEGG pathway analyses of differentially expressed proteins mainly showed involvement in lipid metabolism, oxidative stress, and immune response, which are consistent with pathological characteristics of PCOS in terms of abnormal metabolism, endocrine disorders, chronic inflammation and imbalance between oxidant and antioxidant levels. Also, we found that inflammatory responses were activated in the PCOS ovarium, while lipid biosynthetic process peroxisome, and bile secretion were inhibited. In addition, we found some alteration in unexpected pathways, such as glyoxylate and dicarboxylate metabolism, which should be investigated. The present study makes an important contribution to the current lack of PCOS ovarian proteomic data and provides an important reference for research and development of effective drugs and treatments for PCOS.","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 8","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0