Molecular Reproduction and Development最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148093907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148093908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148093909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of DNA Methylation and ZBTB Transcription Factors in Sex Differentiation, Maintenance and Heat-Induced Masculinization in Zebrafish","authors":"Fabien Pierron,&nbsp;Débora Heroin,&nbsp;Flore Daramy","doi":"10.1002/mrd.70112","DOIUrl":"10.1002/mrd.70112","url":null,"abstract":"<p>Sex differentiation, the process by which an organism develops as a male or female, can be influenced by the environment in many species. High temperature, or pollutant exposure, can lead to skewed sex-ratios and genotype-phenotype mismatches that can seriously threaten populations and biodiversity. Understanding the mechanisms involved in sex differentiation is thus of crucial importance. In the present study, we analyzed the DNA methylation and transcriptional changes that occur during sex differentiation in zebrafish. Our results support the fact that DNA methylation is not only involved in sex differentiation but also in sex maintenance. Initiation of sex differentiation was associated with a DNA demethylation event. Subsequently, a second DNA methylation remodeling event, which coincided with the loss of bi-potentiality, was observed in males. Among the genes under study, the methylation level of the <i>aromatase</i> gene was found to be the most robust sex marker. However, genes encoding for transcription factors of the ZBTB family offered a more precocious marker since sex-specific changes in their transcription levels preceded changes in DNA methylation. In addition, their transcription levels were found be sensitive to a known masculinizing factor, i.e. temperature. Our results thus support an unexpected role of ZBTB proteins in sex differentiation.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148032975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148093906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Garcinol Inhibits the Proliferation of Endometriosis Cells by Regulating Cell Cycle Related Genes and Signaling Pathways","authors":"Jing Liu,&nbsp;Bin Zhao,&nbsp;Yixin Ye,&nbsp;Deqiong Ou,&nbsp;Xiaowen Gan,&nbsp;Miaojuan Chen,&nbsp;Fen Ning,&nbsp;Mingguang Zhao,&nbsp;Zidi Zhang,&nbsp;Hongyang Liu,&nbsp;Yanqing Huang,&nbsp;Miaomiao Du,&nbsp;Li Li,&nbsp;Shuang Qin,&nbsp;Xiaoyan Sha,&nbsp;Ping Li,&nbsp;Lindong Zhang,&nbsp;Gendie E. Lash,&nbsp;Qinsheng Lu","doi":"10.1002/mrd.70111","DOIUrl":"10.1002/mrd.70111","url":null,"abstract":"<div>\u0000 \u0000 <p>Endometriosis is a common gynecological disease with high recurrence rates after surgery and the lesions keep unlimited proliferative capacity. The effect of garcinol on cell proliferation has not been investigated in endometriosis. Herein, we studied the effect of garcinol on endometriosis by using the immortalized human endometriotic epithelial cell line 12Z and stromal cell line iheESCs. RTCA and EdU assays were used to assess cell proliferation. RNA-Seq was used to discover the differential expression gene (DEG) profile and GSEA, KEGG, protein-protein interaction network and the transcription factor interaction network were analyzed. We found that garcinol inhibited the cell proliferation and S phase DNA synthesis of 12Z and iheESCs cells. There were 548 DEGs in the transcription profiles of the two cell lines. The GSEA results showed that garcinol could inhibit the DNA replication and cell cycle pathways. Among the cell cycle related genes, p21 was increased, while cyclin B1, cyclin E1, CDK2, CDK4, Myc, p27 and E2F1 were significantly decreased after garcinol treatment. The level of cell cycle M phase marker, pH3 Ser10, was also reduced by garcinol. Garcinol could regulate the Akt/c-Jun/ERK1/2 signaling pathways. Garcinol could regulate the protein-protein interaction network and the transcription factor interaction network, in which garcinol increased the transcription factor ATF3 and FOSB expression. In conclusion, garcinol could inhibit the proliferation of endometriosis cells in vitro.</p>\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148032916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148093910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148093914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Icariin Improves D-Gal-Induced Sertoli Cell Dysfunction by Activating Autophagy–Lysosomal–Mitochondrial Pathway","authors":"Meihua Wang,&nbsp;Feng Jiang,&nbsp;Xianglong Wang,&nbsp;Dong Niu,&nbsp;Huaming Xi","doi":"10.1002/mrd.70119","DOIUrl":"10.1002/mrd.70119","url":null,"abstract":"<div>\u0000 \u0000 <p>Epimedium brevicornu Maxim (Yinyanghuo) is widely used to treat reproductive disorders. Icariin (ICA), the main active ingredient of Epimedium brevicornu Maxim, can alleviate age-related testicular dysfunction and Sertoli cell injury. However, whether ICA can regulate the autophagic activity of Sertoli cells and thus improve cell function remains unclear. The present study aimed to evaluate the protective effects of ICA on mitochondrial biogenesis, lysosome biogenesis, and autophagic activity in D-Galactose (D-gal)-induced Sertoli cell. TM4 cells were pretreated with ICA and then exposed to D-gal. After treatment, Sertoli cell viability was detected. Oxidative stress, apoptosis, mitochondrial biogenesis, lysosomal biogenesis, and autophagy-related protein and mRNA levels were analyzed. Sertoli cells were stained with Lyso-Tracker and Mito-Tracker to visualize lysosomes and mitochondria. ICA treatment significantly increased the Sertoli cell viability and protected against D-gal-induced oxidative stress. Meanwhile, ICA treatment significantly increased the expression levels of TFEB and LAMP2 proteins and promoted lysosomal biogenesis. Moreover, ICA treatment increased the expression of PINK1, Parkin, ATG5, ULK1, and LC3-II proteins, activated autophagic activity, and thus improved Sertoli cell function and increased GDNF and Claudin5 protein levels. ICA treatment could alleviate oxidative stress, increase mitochondrial biogenesis, and improve Sertoli cell function by activating autophagy and promoting lysosomal biogenesis.</p></div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148032960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bushen Zhuluan Decoction Ameliorates Diminished Ovarian Reserve in Rats via the SIRT1/HIF-1α Pathway","authors":"Chuqiang Shu,&nbsp;Huizhen Liang,&nbsp;Zhizhen Huang,&nbsp;Ling Wang,&nbsp;Ninglian Yang,&nbsp;Xiaoyi Zhou,&nbsp;Anqi Luo,&nbsp;Xinyu Xiang,&nbsp;Qiong Xia","doi":"10.1002/mrd.70117","DOIUrl":"10.1002/mrd.70117","url":null,"abstract":"<div>\u0000 \u0000 <p>Diminished ovarian reserve (DOR) is a major cause of female infertility. In recent years, traditional Chinese medicine has displayed unique ameliorating effects on DOR. This study aims to investigate the therapeutic efficacy and molecular mechanisms of Bushen Zhuluan Decoction (BSZLD) in DOR. A rat model of DOR was established and treated with either BSZLD or Progynova (positive control). Estrous cycle monitoring, ovarian index calculation, hematoxylin-eosin staining, and enzyme-linked immunosorbent assay were performed to assess ovarian reserve function. TUNEL staining, immunohistochemistry, and Western blotting were conducted to evaluate ovarian cell apoptosis. To elucidate the pharmacological basis of BSZLD, ultra-high-performance liquid chromatography-tandem mass spectrometry was applied. Network pharmacology analysis, machine learning algorithms, and molecular docking were integrated to predict key active compounds and pathways. The role of the sirtuin 1 (SIRT1) and its downstream target hypoxia-inducible factor 1alpha (HIF-1α) signaling in BSZLD-mediated effects was verified using short hairpin RNA (shRNA) lentiviral-mediated interference. BSZLD significantly restored estrous cycle disturbances, increased ovarian index and follicle counts, and reduced atretic follicles and ovarian cell apoptosis in DOR rats. Serum anti-Müllerian hormone and estradiol levels were elevated, while follicle-stimulating hormone and luteinizing hormone levels were reduced. The SIRT1/HIF-1α signaling pathway was a core regulatory axis for BSZLD action. BSZLD markedly upregulated SIRT1 expression while suppressing HIF-1α expression. Silencing SIRT1 using shRNA reduced SIRT1 levels, elevated HIF-1α expression, and significantly attenuated the therapeutic effects of BSZLD. BSZLD attenuates DOR in rats by activating the SIRT1/HIF-1α signaling, thereby suppressing ovarian cell apoptosis and restoring ovarian reserve function.</p></div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 5","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148032797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}