Mycoses最新文献

{"title":"Clinical Outcomes Amongst Patients Infected With Candida Auris: A Single-Center Study.","authors":"Sara Al Jabi, Rania El-Lababidi, Emna Abidi, Claude Afif, Wasim S El Nekidy","doi":"10.1111/myc.70054","DOIUrl":"10.1111/myc.70054","url":null,"abstract":"<p><p>Candida auris (C. auris) is a therapeutic challenge due to the lack of definition of susceptibility breakpoints and the misidentification by biochemical tests, which leads to suboptimal therapy. Hence, our goal was to assess the treatment outcomes of C. auris infections at our institution.</p><p><strong>Methods: </strong>A retrospective observational study between January 2019 and June 2022 that included confirmed C. auris infection cases. The primary endpoint was to assess the clinical outcomes of C. auris management. The secondary endpoints were to evaluate mycologic cure, 30 and 90-day infection recurrence, and 30-day all-cause mortality. Descriptive statistics were used to analyse our data.</p><p><strong>Results: </strong>Fifty-six subjects were evaluated, with a mean age of 65.05 ± 16.86 years. Candidemia accounted for 62.7% of cases. Clinical cure was achieved in 57% of patients, and mycologic cure in 84.4%. Recurrence of C. auris infection occurred in 28.6% at 30 days and in 12.7% at 90 days. Thirty-day all-cause mortality occurred in 28.6% of patients. Multivariable logistic regression indicated that mycologic cure with Odds Ratio (OR) 6.96 (95% CI: 1.21-39.92), length in intensive care units (ICU) stay OR 0.132 (95% CI: 0.019-0.907), and baseline C-reactive protein (CRP) OR 0.990 (95% CI: 0.982-0.998) were the independent predictors of clinical cure.</p><p><strong>Conclusion: </strong>Clinical cure of invasive C. auris infections was dependent on mycologic cure, length of ICU stays, and baseline CRP levels, with observed 30-day all-cause mortality up to 28.6%. Similarly to other reports, our isolates exhibited resistance to fluconazole and amphotericin B in most cases. Only two isolates demonstrated resistance to caspofungin and were deemed pan-resistant. Further multi-centre studies are needed to validate our findings.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 4","pages":"e70054"},"PeriodicalIF":4.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolving Phylogenetic Relationships Within the Trichophyton mentagrophytes Complex: A RADseq Genomic Approach Challenges Status of 'Terbinafine-Resistant' Trichophyton indotineae as Distinct Species.","authors":"Michaela Švarcová, Miroslav Kolařík, Yuanjie Li, Clement Kin Ming Tsui, Vít Hubka","doi":"10.1111/myc.70050","DOIUrl":"10.1111/myc.70050","url":null,"abstract":"<p><strong>Background: </strong>The Trichophyton mentagrophytes complex encompasses common dermatophytes causing superficial mycoses in humans and animals. The taxonomy of the complex is unstable, with conflicting views on the species status of some taxa, particularly T. indotineae and T. interdigitale. Due to the presence of intermediate genotypes, neither MALDI-TOF MS nor ITS rDNA sequencing can accurately distinguish all taxa in the complex, potentially contributing to clinical misdiagnoses.</p><p><strong>Objectives: </strong>This research resolves phylogenetic relationships within the T. mentagrophytes complex. Based on these data, the taxonomical recommendations are suggested.</p><p><strong>Methods: </strong>In order to resolve the phylogenetic relationship of the T. mentagrophytes complex, we employed Restriction Site-Associated DNA Sequencing (RADseq) to produce a high-resolution single nucleotide polymorphism (SNP) dataset from 95 isolates. The SNP-based analyses indicated the presence of two major genetic clusters corresponding to T. mentagrophytes (including T. indotineae) and T. interdigitale.</p><p><strong>Results: </strong>Our results challenge the species status of T. indotineae because of insufficient genetic divergence from T. mentagrophytes. Therefore, we propose designating T. indotineae as T. mentagrophytes var. indotineae (or T. mentagrophytes ITS genotype VIII) to avoid further splitting of the complex and taxonomic inflation. Although T. interdigitale shows clearer genetic differentiation, its separation is incomplete and identification of some isolates is ambiguous when using routine methods, leading us to consider it a variety as well: T. mentagrophytes var. interdigitale.</p><p><strong>Conclusions: </strong>We recommend using T. mentagrophytes as the overarching species name for all complex isolates. Where precise molecular identification is possible, the use of variety ranks is encouraged. Since identical resistance mechanisms are not specific to any genotype or dermatophyte species, identifying antifungal resistance is more important than differentiating closely related genotypes or populations.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 4","pages":"e70050"},"PeriodicalIF":4.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Resistance in Non-fumigatus Aspergillus Species.","authors":"Elie Djenontin, Rose-Anne Lavergne, Florent Morio, Eric Dannaoui","doi":"10.1111/myc.70051","DOIUrl":"https://doi.org/10.1111/myc.70051","url":null,"abstract":"<p><p>This review provides an in-depth exploration of antifungal resistance in non-fumigatus Aspergillus species, mainly focusing on acquired resistance. The available data have been compiled and sometimes re-analysed. It highlights the increasing prevalence of resistance in non-fumigatus species belonging to Flavi, Terrei, Nigri, and Nidulantes Aspergillus sections, offering a detailed analysis of resistance detection methods and the global distribution of resistant strains. The review also thoroughly examines the molecular mechanisms behind resistance and raises key unresolved issues, such as the factors contributing to resistance selection and the clinical implications of in vitro resistance. Additionally, it addresses the challenges of treating infections caused by resistant Aspergillus species and cryptic species and discusses current and future strategies relying on combination therapy and newly developed antifungals. The conclusion emphasises the need for further research into resistance mechanisms and alternative treatments to address the rising threat of antifungal resistance in Aspergillus species.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 4","pages":"e70051"},"PeriodicalIF":4.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined ImmunoCAP and Western Blot for the Diagnosis of Aspergillus Lung Disease.","authors":"Emanuel Petit, Sébastien Cortaredona, Stéphanie Sicco, Stéphane Ranque, Estelle Menu","doi":"10.1111/myc.70058","DOIUrl":"https://doi.org/10.1111/myc.70058","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary aspergillosis is a major global health concern, yet its diagnosis remains challenging. Aspergillus-specific IgG measurement is essential for identifying chronic and allergic forms.</p><p><strong>Objective: </strong>This study aimed to evaluate a quantitative method, the ImmunoCAP assay IgG m3 (ICAP) (Phadia-ThermoFisher Scientific, Waltham, USA), a qualitative method, the Aspergillus IgG Western blot kit (Asp-WB) (LDBio Diagnostics, Lyon, France) and a combination of both methods for the diagnosis of Aspergillus lung disease.</p><p><strong>Methods: </strong>A retrospective study was conducted at the University Hospital of Marseille, France, during 1 year. Patients undergoing Aspergillus serology were divided into three groups: Group 1 (G1) with ICAP ≥ 40 mgA/L and positive Asp-WB, Group 2 (G2) with ICAP ≥ 40 mgA/L and negative Asp-WB and Group 3 (G3) with ICAP < 40 mgA/L and positive Asp-WB. Data were collected on demographics, underlying diseases, imaging and biological outcomes. Patients were classified according to their Aspergillus lung disease, whether acute pulmonary aspergillosis, chronic pulmonary aspergillosis (CPA), allergic broncho-pulmonary aspergillosis (ABPA), colonisation or Aspergillus sensitisation.</p><p><strong>Results: </strong>A total of 536 patients were studied: 173 in G1, 204 in G2 and 200 in G3, with 38 patients found in several groups. The primary underlying disease was cystic fibrosis in 44.6% of patients. Twenty-two patients were diagnosed with ABPA. The number of diagnosed ABPA cases in G1 (20; 11.6%) combining positive ICAP and Asp-WB was significantly higher than that found in the groups with a single positive test result (p < 0.001). Fifteen patients were diagnosed with CPA. Isolated positive Western blot (G3) identified five cases of aspergilloma. Significantly fewer Aspergillus lung diseases were diagnosed in isolated positive ICAP G2 (8.8%) than in G1 (53.8%) and G3 (42.5%) (p < 0.001).</p><p><strong>Conclusions: </strong>This study highlights the benefits of combining Asp-WB and ICAP for the diagnosis of Aspergillus lung disease and the relatively high false-positive rate in patients with isolated positive ICAP results.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 4","pages":"e70058"},"PeriodicalIF":4.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11998474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitivity and Specificity of Plasma and Bronchoalveolar Lavage Fluid PCR for Diagnosing Pulmonary Mucormycosis in Subjects With Diabetes Mellitus.","authors":"Rana Sadaqat Nawaz, Ritesh Agarwal, Shivaprakash M Rudramurthy, Hansraj Choudhary, Ritika Harchand, Karthick Kumar, Inderpaul Singh Sehgal, Harsimran Kaur, Sahajal Dhooria, Kuruswamy Thurai Prasad, Nidhi Prabhakar, Ashutosh N Aggarwal, Valliappan Muthu","doi":"10.1111/myc.70063","DOIUrl":"https://doi.org/10.1111/myc.70063","url":null,"abstract":"<p><strong>Background: </strong>Mucorales polymerase chain reaction (PCR) is used to diagnose pulmonary mucormycosis (PM) among neutropenic individuals. However, data on the utility of PCR in patients with diabetes mellitus, another major risk factor for PM, are limited.</p><p><strong>Objective: </strong>The primary objective was to assess the diagnostic performance of a commercial real-time PCR assay (MucorGenius) in plasma and bronchoalveolar lavage fluid (BALF) for diagnosing PM (proven and probable cases only) in patients with suspected invasive mould disease (IMD). For the secondary objective, we evaluated the performance of the MucorGenius assay in all PM (proven, probable, and possible) cases.</p><p><strong>Methods: </strong>We prospectively enrolled patients with suspected IMD and assessed the performance of MucorGenius PCR (index test) in plasma and BALF samples. A multidisciplinary team assigned the final diagnosis of IMD (reference standard) based on microscopy, histopathology, cytology, and culture. We report the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) with 95% confidence intervals (CI).</p><p><strong>Results: </strong>We enrolled 103 patients, of whom 43 (41.7%) were confirmed to have PM. Plasma PCR showed a sensitivity of 18.6% (95% CI: 8.4-33.4), specificity of 90.7% (95% CI:77.9-97.4), PPV of 66.7%, and NPV of 52.7%. Including possible PM/IMD cases improved the plasma PCR sensitivity to 30.0% (95% CI: 18.9-43.2) and retained specificity at 90.7%. BALF PCR had better sensitivity (47.4%) but poorer specificity (69.6%), with a PPV of 56.3% and NPV of 61.5%.</p><p><strong>Conclusion: </strong>Plasma and BALF MucorGenius PCR have poor diagnostic performance for diagnosing PM among individuals with diabetes mellitus. Further multicenter studies are needed to validate these findings.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 4","pages":"e70063"},"PeriodicalIF":4.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Medium That Promotes Mass Sporulation of Trichophyton rubrum In Vitro.","authors":"Hailin Zheng, Wenting Xie, Dongmei Li, Huan Mei, Xiaofang Li, Weida Liu","doi":"10.1111/myc.70039","DOIUrl":"10.1111/myc.70039","url":null,"abstract":"<p><strong>Background: </strong>Trichophyton rubrum is one of the most common human pathogenic fungi, with the infections often managed through empirical drug use. However, treatments frequently lead to relapse upon withdrawal. The rise in drug-resistant T. rubrum cases has led to an increased demand for drug susceptibility assays in clinical settings to guide antifungal drug selection and monitor therapeutic outcomes. However, insufficient sporulation of T. rubrum on routinely-used media presents a significant challenge, limiting the widespread use of drug sensitivity tests on a large scale.</p><p><strong>Objectives: </strong>There is an urgent need to develop a new, effective culture method that can promote rapid and abundant sporulation of T. rubrum.</p><p><strong>Methods: </strong>In this study, we adopted a nutrient-rich medium (Yeast extract-glucose YAG) which demonstrated superior efficacy in promoting T. rubrum sporulation compared to PDA and OA mediums.</p><p><strong>Results: </strong>Abundant microconidia production of T. rubrum was observed at 30° between 7 to 10 days on YAG medium, and this sporulation was further enhanced under elevated carbon dioxide conditions. Particularly, a 20% carbon dioxide environment significantly improved T. rubrum sporulation on PDA by 30-fold, equivalent to the sporulation effect with YAG cultivation.</p><p><strong>Conclusions: </strong>This enhanced sporulation was confirmed in 37 clinical T. rubrum isolates, highlighting the potential applicability of YAG medium in both clinical and research settings for drug susceptibility assays and pathogenesis studies. These findings also offer new insights into improving sporulation in other fungi with low spore production.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 3","pages":"e70039"},"PeriodicalIF":4.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Manifestations and Prognosis of Patients With Mucormycosis in Intensive Care Units in Western China: A Multi-Center Retrospective Study.","authors":"Fuxun Yang, Yi Zhang, Bo Qi, Li Chen, Fang Lin, Jiani Wu, Sihan Gong, Lianghai Cao, Mingquan Zeng, Qiong Cheng, Dexiong Jiang, Shiyuan Tang, Jieming He, Zhihua Xu, Tun Li, Zhen Ni, Yachao Li, Xiaobo Huang, Chun Pan, Rongan Liu, Yunping Lan","doi":"10.1111/myc.70042","DOIUrl":"10.1111/myc.70042","url":null,"abstract":"<p><strong>Background: </strong>Mucormycosis is a life-threatening fungal infection with high mortality in critically ill patients. Clinical manifestations and outcomes of mucormycosis in intensive care units (ICUs) remain poorly investigated.</p><p><strong>Methods: </strong>We conducted a multicenter retrospective study including 43 adult patients with confirmed mucormycosis admitted to 14 tertiary ICUs between January 2014 and May 2022. Clinical characteristics, diagnostic approaches, treatment strategies, and outcomes were analysed.</p><p><strong>Results: </strong>The mean age was 56.8 ± 16.2 years, with 16/43 (37.2%) female patients. The 28-day survival rate was 46.5% (20/43). Lung involvement was predominant (29/43, 67.4%), and 29/43 (67.4%) patients received amphotericin B therapy. Survivors showed significantly better treatment response compared to non-survivors (16/20, 80% vs. 4/23, 17.4%, p < 0.001). Non-survivors demonstrated significantly higher levels of aspartate aminotransferase, C-reactive protein, and white blood cells, along with lower albumin levels. Metagenomic next-generation sequencing (mNGS) was associated with a shorter time to diagnosis. Multivariate analysis identified age, respiratory failure, time from symptom onset to diagnosis, and antifungal treatment response as independent predictors of 28-day mortality (AUC = 0.852).</p><p><strong>Conclusion: </strong>In critically ill patients with mucormycosis, early diagnosis and prompt targeted therapy are crucial determinants of survival, with our newly developed prediction model providing a practical tool for risk stratification, while mNGS shows promise in expediting diagnosis.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 3","pages":"e70042"},"PeriodicalIF":4.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal Infections Associated With TNF-Inhibitors: A 20-Year of a Systematic Review Fungal Infections and TNF-Inhibitors.","authors":"Layala Stefane de Paula Barbosa, Marina Miranda Ely, Túlio Máximo Salomé, Gleyce Hellen de Almeida de Souza, Daniel Assis Santos, Rafael Wesley Bastos, Luana Rossato","doi":"10.1111/myc.70040","DOIUrl":"10.1111/myc.70040","url":null,"abstract":"<p><p>TNF-α inhibitors, including infliximab, adalimumab and etanercept, are used to treat various inflammatory diseases, such as arthritis, psoriasis and ankylosing spondylitis. However, these treatments may predispose patients to fungal infections, including histoplasmosis, candidiasis and aspergillosis. In this study, we systematically reviewed case reports to critically examine the correlations between anti-TNF-α therapies and the occurrence of invasive and superficial fungal infections. Infliximab was the most commonly used TNF-α inhibitor (50.65%). The highest number of fungal infections during anti-TNF34 α therapy was reported in the USA (84.25%). The conditions treated primarily included rheumatoid arthritis. A total of 517 invasive fungal infections were identified, including histoplasmosis, invasive candidiasis and aspergillosis, with histoplasmosis being the most common. Most studies were conducted in higher-income countries, highlighting the critical lack of research on the use of immunobiologicals in relation to fungal diseases in African countries, which requires further attention. Logistic regression analysis revealed significant associations between adalimumab use and increased risks of candidiasis, coccidioidomycosis, onychomycosis and pityriasis versicolor. For etanercept, significant associations were found with aspergillosis, coccidioidomycosis, cryptococcosis, dermatophytosis, invasive candidiasis, pityriasis versicolor and onychomycosis. Infliximab use was significantly associated with coccidioidomycosis, onychomycosis, aspergillosis, cryptococcosis, histoplasmosis and invasive candidiasis. The data presented in this study clearly demonstrate an association between the use of TNF-α inhibitors and an increased risk of fungal infections. It is imperative that healthcare professionals maintain a high level of vigilance when managing patients on these medications. Regular monitoring and proactive management strategies are essential to mitigate risks and ensure patient safety.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 3","pages":"e70040"},"PeriodicalIF":4.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key Factors to Consider for Candida auris Screening in Healthcare Settings: A Systematic Review.","authors":"Anders Skyrud Danielsen, Liz Ertzeid Ødeskaug, Ragnhild Raastad, Anne Kjerulf, Anne-Marie Andersen, Ragnhild Agathe Tornes, Jan P W Himmels, Ulf R Dahle, Miriam Sare, Brian Kristensen, Hanne-Merete Eriksen-Volle, Mari Molvik","doi":"10.1111/myc.70043","DOIUrl":"10.1111/myc.70043","url":null,"abstract":"<p><strong>Background: </strong>Candida auris is an emerging fungal pathogen that is often multidrug-resistant. It can persist on skin and in hospital environments, leading to outbreaks and severe infections for patients at risk. Several countries and institutions are working on establishing guidelines and recommendations for prevention. This review aims to assess the evidence on factors associated with C. auris colonisation or infection, the duration of such colonisation, possible colonisation sites, and the risk of secondary cases to inform screening recommendations.</p><p><strong>Methods: </strong>We systematically searched five databases for primary studies and systematic reviews of our four outcomes. We excluded studies on treatment, management, laboratory methods, drug resistance, and environmental screening. From each paper, we extracted relevant data and summarised them in tables. Main findings were described narratively.</p><p><strong>Findings: </strong>We selected 117 studies for inclusion. Most of the studies were observational studies. Without taking the method of testing into account, the duration of C. auris colonisation varied, with up to and beyond a year being common. The predominant sites of colonisation were the axillae and groin, with the nares and rectum being less common sites. The risk of secondary cases saw considerable variation across the studies, and the secondary cases primarily involved patients and not healthcare workers. Critical care settings, invasive medical devices, recent antimicrobial use, and comorbidities were often associated with C. auris colonisation and infection.</p><p><strong>Conclusion: </strong>Our review highlights that, despite relevant findings on factors influencing C. auris colonisation and infection, substantial gaps remain in the evidence supporting screening practices. Most studies were conducted reactively, in outbreak settings, and lack systematic protocols. Given these limitations, screening guidelines are likely to be more successful if grounded in medical theory and yeast microbiology rather than relying solely on current studies. Rigorous, well-designed research is urgently needed to inform future C. auris screening and control efforts.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 3","pages":"e70043"},"PeriodicalIF":3.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Insights on Transmission of Sporothrix brasiliensis.","authors":"Regielly C R Cognialli, Flávio Queiroz-Telles, Alyson M Cavanaugh, Bruno C Rediguieri, Giovanna C D Santos, Felipe Moreira Matias, Luciano Moreira Filho, Elias N Monteiro Neto, Mônica Surek, Vânia A Vicente, Izabella C R Santos-Weiss","doi":"10.1111/myc.70047","DOIUrl":"10.1111/myc.70047","url":null,"abstract":"<p><strong>Background: </strong>Sporotrichosis is the most prevalent saprozoonotic implantation mycosis worldwide.</p><p><strong>Objectives: </strong>A prospective experimental study was performed to evaluate persistence on surfaces such as stainless steel medium-density fiberboard (MDF) wood, tile, and polyester fabric.</p><p><strong>Methods: </strong>The different surfaces were homogeneously contaminated using the yeast phase of Sporothrix brasiliensis. Fungal culture was carried out in triplicate on Mycosel agar, at time 0 and then every 24 h, until 792 h (33 days).</p><p><strong>Results: </strong>Sporothrix brasiliensis persists on MDF wood for up to 240 h (10 days), tile for 288 h (12 days), polyester fabric for 384 h (16 days), and stainless steel for up to 600 h (25 days).</p><p><strong>Conclusions: </strong>Based on these findings, in concordance with published clinical reports suggesting fomite transmission, we propose an updated schematic of S. brasiliensis transmission. This is the first in vitro study about the persistence of S. brasiliensis on different surfaces, which is essential to understand transmission routes for a One Health Approach.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 3","pages":"e70047"},"PeriodicalIF":3.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}