Mycoses最新文献

{"title":"Integrated Genomic and Phenotypic Analyses Reveal Convergent Resistance Patterns in Clinical Candida tropicalis Isolates.","authors":"Christy Chedraoui, Nour Fattouh, Setrida El Hachem, Tsolaire Sourenian, Louna El Bitar, Lama El Moheb, Anna-Souraya Sleiman, Ibrahim Bitar, Rola Husni, Roy A Khalaf","doi":"10.1111/myc.70181","DOIUrl":"https://doi.org/10.1111/myc.70181","url":null,"abstract":"<p><strong>Background: </strong>The incidence of Candida tropicalis isolation is increasing in hospital settings. High azole resistance and mortality rates make it a pathogen that requires further analysis.</p><p><strong>Methods: </strong>Fourteen azole resistant Candida glabrata clinical isolates were collected from a Lebanese hospital and analysed through whole genome sequencing for single nucleotide polymorphisms in key resistance and virulence genes, and for phylogenetic relatedness. Isolates were then characterised for pathogenicity related attributes.</p><p><strong>Results: </strong>All isolates had Lys314Glu mutation in ERG20 with multiple isolates displaying numerous shared mutations, such as Glu291Lys in CDR2 and Ala16Thr in CDR3. With the exception of two isolates that clustered together, most isolates were over 99.6% identical based on a genomic heatmap, implying high relatedness consistent with localised clonal expansion, although SNP differences appeared too high to support this. However, the isolates exhibited increased ergosterol and chitin content, as well as upregulation of drug efflux pumps resulting in drug resistance.</p><p><strong>Conclusion: </strong>Our hospital isolates showed convergent resistant pathways, with many isolates having both shared and unique mutations and a high degree of genomic similarities.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70181"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histoplasma Urinary Antigen for the Diagnosis Histoplasmosis in Non-HIV Individuals.","authors":"João Nobrega de Almeida, Tamara Harb Roca, Denise Dos Anjos Laurentis de Souza Campos, Renée Zon Filippi, Cristóvão Luis P Mangueira, Marines Dalla Valle Martino","doi":"10.1111/myc.70183","DOIUrl":"10.1111/myc.70183","url":null,"abstract":"<p><strong>Background: </strong>Histoplasmosis is an endemic mycosis in the Americas. While urinary Histoplasma antigen enzyme immunoassay testing (HAET) has been validated mainly in HIV-infected patients with disseminated disease, its performance in HIV-negative populations with heterogeneous clinical presentations is unclear.</p><p><strong>Objectives: </strong>To describe the performance of urinary HAET for the diagnosis of histoplasmosis in a cohort of Brazilian HIV-negative patients.</p><p><strong>Material and methods: </strong>We conducted a retrospective study at Einstein Hospital Israelita (Brazil) including HIV-negative patients whose urine samples were analysed by Histoplasma antigen enzyme immunoassay test (HAET) from January 2022 to October 2025. Factors associated with positive urinary HAET were analysed using univariable and multivariable models. Among 346 patients, histoplasmosis was diagnosed in 43 (12%); 16 (37%) were immunocompromised and 14 (33%) had disseminated disease. Urinary HAET was positive in eight patients (19%), with antigen levels from 0.3 to > 15 ng/mL. Sensitivity and specificity were 19% and 100%, respectively; positive and negative predictive values were 100% and 90%. Sensitivity increased to 45% in immunocompromised patients with multiorgan involvement, and immunocompromised status independently predicted HAET positivity (p = 0.008). In HIV-negative patients, HAET demonstrates excellent specificity but low sensitivity, performing best in immunocompromised individuals with disseminated histoplasmosis.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70183"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13146122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental Circulation of Aspergillus fumigatus With Reduced Susceptibility to Agricultural Triazole in Brazil: Clonal Dissemination of Potentially Resistant Genotypes.","authors":"Dality Keffelen de Barros Rodrigues, Manuel Leeuwerik, Balázs Brankovics, Wellington Santos Fava, James Venturini, Wieland Meyer, Teppei Arai, Hidetaka Majima, Akira Watanabe, Marcia de Souza Carvalho Melhem","doi":"10.1111/myc.70179","DOIUrl":"https://doi.org/10.1111/myc.70179","url":null,"abstract":"<p><strong>Background: </strong>Aspergillus fumigatus resistance to triazole antifungals poses an increasing global health concern. Moreover, the cross-resistance between azole antifungal agents used in clinical settings and those applied in agriculture has become an important emerging issue.</p><p><strong>Objectives: </strong>In this study, we investigated the five environmental A. fumigatus strains showing reduced susceptibility to tebuconazole.</p><p><strong>Methods: </strong>Fungal strains were recovered from air samples collected around the homes of two patients with suspected aspergillosis caused by resistant isolates. Species identification was performed by sequencing the β-tubulin gene, and minimum inhibitory concentrations were determined by broth microdilution. The cyp51A gene was sequenced to detect mutations, and CRISPR-Cas9 genome editing was employed to investigate their influence on susceptibility patterns. Microsatellite genotyping was performed to assess genetic variability, followed by whole genome sequencing and single nucleotide polymorphism analysis.</p><p><strong>Results: </strong>The environmental strains presented the same cyp51A genotype characterised by the M172V substitution and silent mutations. Microsatellite genotyping and whole genome sequencing confirmed that the strains were clonal. Functional validation demonstrated that the M172V and silent mutations partially contribute to reduced susceptibility to tebuconazole but are not the main mechanism of resistance involved. Analysis of polymorphisms in genes other than cyp51A revealed no resistance-conferring mutations.</p><p><strong>Conclusions: </strong>The findings described herein suggest the possibility of local clonal dissemination of environmental strains under selective pressure from agricultural azoles in a major agribusiness region of the Midwest of Brazil. This study highlights the silent spread of potentially resistant genotypes in urban areas and reinforces the need for environmental surveillance and expanded genomic monitoring in South America.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70179"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Patterns of Osteoarticular, Bone Marrow and Urogenital Paracoccidioidomycosis.","authors":"Wdson Luis Lima Kruschewsky, Mariane Cappellini Stricagnolo, Mônica Scarpelli Martinelli Vidal, Gil Benard, Vítor Falcão de Oliveira, Tiago Alexandre Cocio, Adriana Satie Gonçalves Kono Magri, Marcello Mihailenko Chaves Magri","doi":"10.1111/myc.70182","DOIUrl":"https://doi.org/10.1111/myc.70182","url":null,"abstract":"<p><strong>Background: </strong>Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America, classically characterized by pulmonary, mucosal and lymphatic involvement. Extrapulmonary manifestations affecting uncommon organs remain poorly characterized and may contribute to diagnostic delay and adverse outcomes.</p><p><strong>Methods: </strong>We conducted a retrospective case series of proven or probable atypical extrapulmonary PCM diagnosed at a Brazilian tertiary referral center between 2006 and 2025. A systematic review registered in PROSPERO and conducted according to PRISMA recommendations identified osteoarticular, bone marrow and urogenital PCM cases between 1970 to November 2025 in PubMed/MEDLINE and Embase without language restrictions.</p><p><strong>Results: </strong>Among 205 PCM patients evaluated at our institution, 14 (6.8%) presented atypical extrapulmonary involvement. Combined with 153 cases identified in 75 studies, 167 cases were analysed. Osteoarticular involvement was the most frequent manifestation (53.9%), predominantly affecting young patients with acute/subacute disease and presenting mainly as osteomyelitis with lytic lesions. Bone marrow involvement occurred exclusively in acute/subacute PCM and was associated with post-mortem diagnosis (9.7%) and markedly elevated mortality (77.8%). Urogenital PCM mainly affected middle-aged men with chronic disease, frequently involving the testicles and prostate, and was associated with delayed diagnosis, surgical intervention (32.7%), and mortality (32.0%). Distinct epidemiological profiles, clinical forms and outcomes were consistently observed across atypical sites.</p><p><strong>Conclusion: </strong>Atypical extrapulmonary PCM manifestations follow recognizable clinical patterns associated with diagnostic delay and poor outcomes, supporting earlier investigation and timely antifungal therapy in endemic settings.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70182"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive Fungal Infections After Intestine Transplantation: Epidemiology and Outcomes.","authors":"Mayyadah H Alabdely, Jessica Lum, Blanca E Gonzalez, Masato Fujiki, Zachary A Yetmar","doi":"10.1111/myc.70180","DOIUrl":"https://doi.org/10.1111/myc.70180","url":null,"abstract":"<p><strong>Background: </strong>Invasive fungal infections (IFIs) represent a major complication after intestine transplantation, with reported incidence rates between 40% and 49%. These infections are associated with high morbidity and allograft loss. This study evaluates the impact of post-transplant IFIs on graft outcomes in intestine transplant recipients.</p><p><strong>Methods: </strong>We conducted a retrospective cohort study of 152 patients who underwent intestine transplantation from 2008 to 2022. The primary outcome was IFI, defined as proven or probable by EORTC/MSGERC criteria. The secondary outcome was a composite of allograft failure or death. Analyses were conducted using multivariable Cox proportional hazards models, with non-baseline variables incorporated as time-dependent exposures.</p><p><strong>Results: </strong>Among 152 intestine transplant recipients, 56 (36.8%) developed post-transplant IFI. Median time to IFI was 83.5 days (IQR 19.5-444.2), with Candida infections occurring early and non-Candida infections occurring later. Candida species accounted for 73% of IFIs, most commonly C. glabrata, C. albicans, and C. parapsilosis, with 29.3% showing fluconazole resistance. Most infections were intra-abdominal and bloodstream. Redo transplantation (HR 2.44, 95% CI 1.20-4.95; p = 0.014), anastomotic leak (HR 2.23, 95% CI 1.02-4.90; p = 0.045), and augmented immunosuppression (HR 4.73, 95% CI 1.94-11.53; p < 0.001) were independent predictors of Candida IFI. IFI was associated with a markedly increased risk of allograft loss or death (HR 3.67, 95% CI 2.30-5.83; p < 0.001).</p><p><strong>Conclusions: </strong>Post-transplant IFIs are common and associated with allograft loss or mortality in intestine transplant recipients. Early recognition and aggressive management of IFIs remain critical to improving transplant outcomes.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70180"},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of Mucormycosis: Current Situation, Challenges and Future Prospects.","authors":"Yixuan Qu, Yuling Xiao, Lina Liu, Junyan Qu","doi":"10.1111/myc.70175","DOIUrl":"10.1111/myc.70175","url":null,"abstract":"<p><p>Mucormycosis, an aggressive fungal infection caused by members of the order Mucorales, progresses rapidly and is associated with high mortality, particularly in immunocompromised hosts such as patients with uncontrolled diabetes, transplant recipients or those with COVID-19-associated immunosuppression. Early diagnosis remains challenging with current clinical methods, yet it is essential to reduce mortality. This review examines the evolution of diagnostic strategies for mucormycosis, ranging from conventional techniques such as histopathology, culture and microscopy to advanced and emerging methods including molecular assays, serological testing, imaging and metabolomics. We also explore the ongoing transition towards integrated, rapid and non-invasive diagnostic platforms that leverage novel biomarkers, portable devices and artificial intelligence. These new technologies have the potential to facilitate early diagnosis, thereby enabling early treatment and reducing the mortality rate of mucormycosis.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70175"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T. indotineae: A New Emergent Fungal Pathogen Driven by Global Travel.","authors":"Bryan Ortiz, Florent Morio, Kateryn Aguilar, Franklin García, Gustavo Fontecha, Alicia Moreno-Sabater","doi":"10.1111/myc.70170","DOIUrl":"10.1111/myc.70170","url":null,"abstract":"<p><strong>Background: </strong>The global spread of Trichophyton indotineae, a multidrug-resistant dermatophyte, remains insufficiently understood due to limited data on migration-associated dissemination and emerging local transmission. This study reviewed available evidence and integrated international migration statistics to assess the worldwide distribution of this pathogen and the influence of human mobility on its expansion.</p><p><strong>Methods: </strong>A systematic search in Scopus and PubMed (September 9, 2025) included epidemiological studies, case reports, and letters. Extracted variables comprised country of diagnosis, probable country of infection, and reported transmission scenarios. Migration data from the International Organization for Migration were incorporated to explore links between population movements and fungal spread, including indirect or undocumented routes.</p><p><strong>Results: </strong>Of the 224 publications identified, 89 met the inclusion criteria, reporting a total of 1215 cases outside India across 36 countries. Europe accounted for 545 cases, followed by North America with 90 cases and Oceania with 87 cases, while South America and Africa reported only sporadic detections. Fifty studies described imported infections, whereas 11 documented autochthonous transmission. Integrated analysis revealed three primary dissemination corridors: intra-Asian, Asia-Europe, and Asia-America.</p><p><strong>Conclusions: </strong>Trichophyton indotineae is now globally disseminated and strongly shaped by travel and migration. Coordinated international action, standardized diagnostics, harmonized treatment guidelines, and integrated surveillance are urgently required to mitigate its expanding public health impact worldwide.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70170"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13033971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Epidemiology and Infection Control of Candida Auris in Shanghai.","authors":"Mingjing Wei, Jian Guo, Li Li, Junhao Zhu, Yuyuan Xue, Guo Chen, Fan Yang, Wenjuan Wu, Min Zhu","doi":"10.1111/myc.70176","DOIUrl":"10.1111/myc.70176","url":null,"abstract":"<p><strong>Background: </strong>Candida auris is a globally emerging multidrug-resistant fungus. Its dissemination is driven by patient movement and asymptomatic carriers. However, molecular epidemiological studies linking imported cases to local strains in large metropolitan areas remain limited.</p><p><strong>Objectives: </strong>To analyse the molecular epidemiology and resistance mechanisms of 33 clinical isolates in Shanghai and propose an integrated infection control strategy tailored to metropolitan healthcare settings.</p><p><strong>Methods: </strong>Isolates from 19 patients were identified via MALDI-TOF MS and sequencing. Antifungal susceptibility and biofilm formation were assessed. Environmental contamination was evaluated using PCR. Whole-genome sequencing (WGS) was employed to identify resistance mutations and perform phylogenetic analysis.</p><p><strong>Results: </strong>All 33 isolates were fluconazole-resistant. Resistance to amphotericin B and echinocandins was observed in 9 and 4 isolates, respectively. All echinocandin-resistant strains harboured the Fks1-S639F mutation. Phylogenetic analysis revealed that Clade III (South African clade) was the dominant lineage. Colonisation was concentrated in the urinary tract and inguinal regions, while bedside tables were identified as key environmental reservoirs. All strains exhibited strong biofilm-forming capacity.</p><p><strong>Conclusions: </strong>ICU patients serve as primary reservoirs, with transmission driven by interregional movement. The distinct colonisation patterns and environmental hotspots provide specific targets for screening. Integrating PCR-based environmental monitoring with culture methods constitutes an effective strategy. These findings underscore the need for a standardised protocol integrating molecular diagnostics to optimise infection control in urban hospitals.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70176"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Hyphal Suspension Approach for MIC Testing of Mould Fungi.","authors":"Hailin Zheng, Dongmei Li, Naicen Ge, Huan Mei, Xiaofang Li, Weida Liu","doi":"10.1111/myc.70177","DOIUrl":"https://doi.org/10.1111/myc.70177","url":null,"abstract":"<p><strong>Background: </strong>Filamentous fungi cause a wide range of superficial and invasive infections, and antifungal susceptibility assay is essential for guiding effective therapy. Standard broth microdilution methods rely on microconidia-based inocula; however, many clinically important moulds produce insufficient microconidia due to intrinsic characteristics or culture conditions, limiting the applicability of conventional testing and often necessitating empirical treatment.</p><p><strong>Objectives: </strong>To overcome this limitation, we developed an alternative method using precisely quantified mycelial cell suspensions for MIC determination.</p><p><strong>Methods: </strong>The method was evaluated using 115 fungal strains and compared with traditional microconidia-based testing.</p><p><strong>Results: </strong>High concordance was observed between the two approaches, with agreement rates of 90% or higher across three common dermatophytes causing tinea infections and three representative fungi responsible for invasive infections.</p><p><strong>Conclusions: </strong>This mycelial-based approach provides a reliable and practical alternative for antifungal susceptibility testing of poorly sporulating moulds and may improve the efficiency and accessibility of MIC testing in clinical laboratories.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70177"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence Screening of Chronic Aspergillus Infection in a Post-Tuberculosis Cohort in Senegal: A Cross-Sectional Study Comparing ELISA and Rapid Diagnostic Tests.","authors":"Touré Mariama, Ndiaye Ibrahima Mbaye, Sow Djiby, Diallo Mamadou Alpha, Diongue Khadim, Seck Mame Cheikh, Ndiaye Mouhamadou, Ndiaye Daouda, Ndiaye Faly Diop, Fortes Louise, Denning David, Badiane Aida Sadikh","doi":"10.1111/myc.70174","DOIUrl":"10.1111/myc.70174","url":null,"abstract":"<p><strong>Background: </strong>Chronic pulmonary aspergillosis (CPA) is a significant, yet often overlooked, sequela of pulmonary tuberculosis (TB), particularly in resource-limited settings. Data on the seroprevalence of Aspergillus infection in Senegal is absent, and diagnostic capacity is limited. This study aimed to determine the seroprevalence of Aspergillus-specific antibodies among patients with a history of TB in Senegal and to evaluate the performance of a Rapid Diagnostic Test (RDT) against an Enzyme-Linked Immunosorbent Assay (ELISA).</p><p><strong>Methods: </strong>A cross-sectional study was conducted between April 30 to August 31, 2025 at two health centers in Senegal: Wakhinane and Yeumbeul among patients presenting with chronic respiratory symptoms. After obtaining informed consent from adult participants or from parents/guardians for minors, a blood sample was collected from each participant and tested for Aspergillus antibodies using both a rapid diagnostic test (RDT) and an ELISA. Results were classified as positive, negative, invalid, or not tested. Prevalence and agreement between the two assays were calculated using valid results only. Demographic data were collected, and descriptive statistics with test performance analyses were conducted.</p><p><strong>Results: </strong>The overall seroprevalence was significantly higher by ELISA (11.9%; 38/320) than by RDT (5.5%; 11/201). Site-specific analysis revealed disparities: Wakhinane showed higher RDT positivity (4.5% vs. 1.0%), while Yeumbeul had higher ELISA positivity (13.5% vs. 11.2%). The cohort was predominantly male (66.0%) with a median age of 30 years. ROC analysis of the ELISA identified an optimal threshold that balanced sensitivity (78%) and specificity (89%).</p><p><strong>Conclusion: </strong>This study provided the first serological evidence of substantial Aspergillus antibody prevalence among post-TB patients in Senegal, suggesting a significant burden of undiagnosed CPA. The higher sensitivity of ELISA makes it essential for surveillance and confirmation, while the RDT's practicality offers a viable option for initial screening in peripheral clinics. These findings underscored the urgent need to integrate CPA diagnosis into routine post-TB care and to strengthen fungal diagnostic capacity in West Africa.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70174"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13069597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147662844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}