Mycoses最新文献

{"title":"Diversity and Antifungal Susceptibility of Malassezia spp. Isolated From Brazilian Patients With Pityriasis Versicolor and Seborrheic Dermatitis.","authors":"Diogo Coelho de Pádua Oliveira, Ana Paula Possa, Ana Raquel de Oliveira Santos, Ana Kleiber P Borges, Patrícia Silva Cisalpino, Raquel Vilela, Carlos Augusto Rosa, Susana Johann","doi":"10.1111/myc.70171","DOIUrl":"10.1111/myc.70171","url":null,"abstract":"<p><strong>Background: </strong>Malassezia spp. are part of the microbiota of many animals, including humans. However, under certain conditions, they can become pathogenic. Diseases associated with Malassezia include pityriasis versicolor (PV), seborrheic dermatitis (SD), Malassezia folliculitis, atopic dermatitis, psoriasis and fungemia.</p><p><strong>Objective: </strong>The present study aimed to describe the distribution of Malassezia species among Brazilian patients with PV and SD and to evaluate their susceptibility profiles to common antifungals.</p><p><strong>Methods: </strong>In this study, 102 clinical samples from patients with PV or SD were analysed. Clinical isolates of Malassezia were identified at the species level by sequencing the D1/D2 variable domains of the large subunit rRNA gene. Antifungal susceptibility was assessed using a modified microbroth dilution method adapted for the growth of Malassezia species.</p><p><strong>Results: </strong>Among the 40 cultures obtained, six Malassezia species were identified. M. furfur was the most prevalent species (40.0%), followed by M. sympodialis (27.5%), M. globosa (15.0%), M. japonica (7.5%) and both M. yamatoensis and M. slooffiae (5.0% each). All isolates exhibited high MICs to caspofungin (> s16 μg/mL) and to isoconazole (MIC₅₀ = 8 μg/mL). Miconazole and clotrimazole showed MIC₅₀ values of 4 μg/mL, while itraconazole and ketoconazole were more active, with an MIC₅₀ of 0.125 μg/mL.</p><p><strong>Conclusion: </strong>This study showed the diversity of Malassezia species causing PV and SD in Brazil, including the rare species M. yamatoensis and M. japonica. These findings highlight the importance of antifungal susceptibility testing for these species to guide appropriate therapy.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70171"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13032048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Fluconazole-Resistant Candida parapsilosis Harbouring the G458S ERG11p Substitution in a Brazilian Hospital.","authors":"Sarah Santos Gonçalves, Simone Bravim Maifrede, Eduardo Yäkel, Pedro Massaroni Peçanha, Francisco Ozório Bessa-Neto, Rodrigo Cayô, Anderson Messias Rodrigues, Patricia Muñoz, Pilar Escribano, Jesús Guinea","doi":"10.1111/myc.70160","DOIUrl":"10.1111/myc.70160","url":null,"abstract":"<p><strong>Background: </strong>Fluconazole-resistant Candida parapsilosis is a matter of concern.</p><p><strong>Objectives: </strong>To investigate fluconazole resistance among Candida parapsilosis complex isolates from patients admitted to three Brazilian hospitals, and to characterise resistance mechanisms and clonal relatedness.</p><p><strong>Patients/methods: </strong>A total of 76 C. parapsilosis complex isolates were collected from 60 patients hospitalised at three medical centres (H1-H3) located in Vitória, Espírito Santo state (2017-2023). Of these, 38 isolates were obtained from bloodstream cultures, four from catheter tips and 34 from an ICU surveillance study conducted at H1. In vitro susceptibility testing was performed according to the E.Def 7.4 EUCAST method. C. parapsilosis sensu stricto isolates were genotyped using microsatellite markers, and the ERG11 gene was sequenced in resistant isolates.</p><p><strong>Results: </strong>Candida parapsilosis sensu stricto was the most common species (65/76 isolates; 85.5%), followed by C. orthopsilosis (n = 8, 10.5%), and C. metapsilosis (n = 3, 4%). Among the C. parapsilosis sensu stricto isolates, 5/65 (7.7%) were fluconazole-resistant, harboured the G458S ERG11p substitution, and were detected in 2019 and 2020. Only one fluconazole-resistant isolate was also voriconazole-resistant; all isolates were susceptible to the remaining agents. Fluconazole-resistant isolates sourced from H2 were grouped into two unrelated genotypes, and recovered from patients with haematological malignancies, tumours and central nervous system disorders. Only two patients had a history of azole exposure.</p><p><strong>Conclusions: </strong>This is the first report of fluconazole-resistant C. parapsilosis sensu stricto isolates harbouring the G458S ERG11p substitution in Brazil. Here reported isolates showed resistance to fluconazole alone, which is an unusual pattern among C. parapsilosis sensu stricto isolates harbouring the G458S substitution.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70160"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12958792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Outcomes of Intra-Abdominal Candidiasis by Initial Antifungal Therapy.","authors":"M Albanell-Fernández, A Vergara, F Marco, S Herrera, M Tuset, A Soriano, M Bodro","doi":"10.1111/myc.70147","DOIUrl":"10.1111/myc.70147","url":null,"abstract":"<p><strong>Background: </strong>Intra-abdominal candidiasis (IAC) is a severe and heterogeneous infection associated with significant morbidity and mortality.</p><p><strong>Objectives: </strong>To evaluate outcomes of IAC according to initial antifungal therapy and to identify predictors of persistent infection and 30-day mortality.</p><p><strong>Methods: </strong>A retrospective non-randomised single-centre study (January 2020-February 2025) evaluated adult IAC according to the initial antifungal (azoles vs. echinocandins), assessing demographics, type, location, and persistent positive cultures, re-intervention, antifungal reintroduction, and 3- and 90-day mortality. Multivariate logistic regression identified predictors of persistent cultures and 30-day mortality, and inverse probability of treatment weighting (IPTW) addressed confounding in mortality analyses.</p><p><strong>Results: </strong>Among 154 patients (median age 64.5 years, 66.9% male), 102 received azoles and 52 echinocandins, with greater severity in the latter group. Persistent positive cultures occurred in 51.7% (46/89), without difference between groups. Factors associated with persistent positive cultures included concomitant candidemia, septic shock, previous antibiotic usage, and peritonitis. The 30-day mortality was 28.1% (higher with echinocandins, 38.5% vs. 22.8% in azole group, p = 0.041). Multivariate logistic regression and IPTW indicated that the initial antifungal therapy did not affect 30-day mortality, while septic shock (OR:2.2, 95% CI:1.0-4.9; p = 0.047) and age ≥ 60 years (OR:2.6, 95% CI:1.1-6.3; p = 0.032) were significantly associated with it.</p><p><strong>Conclusions: </strong>IAC remains a complex infection with substantial morbidity and mortality. Echinocandins are preferred in critically ill patients, but mortality did not differ significantly between initial echinocandin and azole treatment. Persistent positive cultures were linked to severe presentation, including peritonitis, candidemia, and septic shock, which required more frequent re-intervention, and consequently carried higher mortality.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70147"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12955698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azole Resistance in Candida parapsilosis From Patients With Burns in Mexico: A Genomic and Phylogenetic Analysis.","authors":"María de Lourdes García-Hernández, Luis Ángel Nuñez-García, Jossue Ortíz-Álvarez, Marco Antonio Delaye-Martínez, Marlen Flores-Huacuja, María Del Rocío Vazquez-Olivares, Luis Fernando Espinosa-Camacho, Gabriela Delgado-Sapien, María Del Rosario Morales-Espinosa, Noé Becerra-Lobato, Rafael Franco-Cendejas, Elvira Garza-González, Luis Ostrosky-Zeichner, Tatiana Chávez-Heres, Melissa Hernández-Durán, Luis Esaú López-Jácome, Claudia Adriana Colín-Castro","doi":"10.1111/myc.70161","DOIUrl":"10.1111/myc.70161","url":null,"abstract":"<p><strong>Background: </strong>Candida species are common causes of nosocomial infections. Candida parapsilosis has emerged as an important pathogen. Mortality rates associated with invasive C. parapsilosis infections range from 14.5% to 47%, with azole resistance estimated between 4% and 15%. Resistance mechanisms include mutations in ERG11 that diminish azole binding, upregulation of ergosterol biosynthesis genes due to mutations in regulatory factors like UPC2 and NDT80, and increased efflux pump activity.</p><p><strong>Methods: </strong>Thirty clinical strains of the C. parapsilosis complex were isolated from bloodstream cultures, biopsies and catheter tips from hospitalised patients from a major rehabilitation centre in Mexico between June 2011 and August 2016. Minimum inhibitory concentrations for fluconazole, voriconazole, itraconazole, caspofungin, micafungin, anidulafungin and amphotericin B were determined using the broth microdilution method. Whole genome sequencing was conducted using Illumina technology, with subsequent assembly and annotation. Analyses included antifungal resistance variant screening, SNP distance, average nucleotide identity and phylogenetic comparisons with publicly available genomes.</p><p><strong>Results: </strong>Most strains (96.7%, 29/30) were identified as C. parapsilosis sensu stricto, predominantly isolated from blood. Thirty-three percent of isolates showed phenotypic resistance to fluconazole. The SNP-distance and maximum-likelihood phylogenetic analyses revealed two distinct haplotypes and the genotype 1: ERG11^WT/Y132F, CDR1^{I1287V/I1287V} and UPC2^WT/N455D was the most represented (8/10 isolates).</p><p><strong>Conclusions: </strong>C. parapsilosis sensu stricto was the predominant species isolated, with a notable proportion exhibiting resistance to FLU and VOR. The Y132F variant in the ERG11 gene was present in most sequenced isolates.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70161"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12966976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Volume Culture Improves Diagnostic Yield of Aspergillus Species From Sputum.","authors":"Merlijn H I van Haren, Laura Michon, Paul E Verweij, Monique H E Reijers, Roger J Brüggemann, Sacha van Mook, Henrich van der Lee, Willem J G Melchers, Suzan D Pas, Jochem B Buil","doi":"10.1111/myc.70168","DOIUrl":"10.1111/myc.70168","url":null,"abstract":"<p><strong>Objectives: </strong>Sputum is a commonly collected specimen for suspected respiratory fungal infections due to its non-invasive nature and diagnostic value. However, conventional culture at 29°C (CC29) often fails to detect filamentous moulds. To improve recovery, we applied a high-volume culture incubated at 37°C (HVC37) and compared its diagnostic performance with CC29, with particular focus on Aspergillus species and azole-resistant isolates.</p><p><strong>Methods: </strong>Between October 2022 and June 2025, a total of 1546 sputum samples from 793 patients, including 219 samples from 67 patients with chronic pulmonary aspergillosis (CPA), were cultured in parallel using HVC37 (0.5-1 mL inoculum) and CC29 (1-10 μL inoculum) on Sabouraud agar for 7 days. Positivity rates for moulds and Aspergillus species were calculated with 95% confidence intervals. Antifungal susceptibility testing followed EUCAST standards.</p><p><strong>Results: </strong>High-volume culture incubated at 37°C (HVC37) markedly outperformed CC29 for detection of both moulds and Aspergillus species. Across all sputum samples, HVC37 achieved a mould positivity rate of 41.6% (95% CI 39.2%-44.1%) versus 27.7% (95% CI 25.5%-30.0%) with CC29. The improvement was most evident for Aspergillus detection: 37.0% (95% CI 34.7%-39.5%) for HVC37 compared with 11.5% (95% CI 10.0%-13.2%) for CC29, an absolute increase of 25.5%. Both methods together yielded the highest overall detection rates for moulds (54.0%) and Aspergillus species (42.1%). In patients with CPA, HVC37 detected moulds in 53.0% of samples and Aspergillus species in 45.2%, compared with 30.6% and 15.5%, respectively, for CC29. Importantly, of 46 azole-resistant A. fumigatus isolates recovered, HVC37 detected 42 (91.3%), whereas CC29 detected only 20 (43.5%). CC29 mainly identified environmental moulds such as Penicillium species, not detected by HVC37.</p><p><strong>Conclusions: </strong>High-volume culture at 37°C substantially improves the detection of filamentous moulds in sputum, especially Aspergillus species and clinically relevant azole-resistant A. fumigatus. Incorporating HVC37 into routine mycology workflows can significantly enhance fungal diagnosis and antifungal resistance surveillance.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70168"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13003582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocide Response of Candida auris.","authors":"Sidre Erganis, Ali Ozturk, Elif Ayca Sahin, Ayse Kalkanci","doi":"10.1111/myc.70169","DOIUrl":"10.1111/myc.70169","url":null,"abstract":"<p><p>Candida auris is an emerging multidrug-resistant yeast demonstrating remarkable persistence in healthcare environments, contributing to nosocomial transmission and outbreak persistence. Increasing disinfectant failure reports have raised concerns regarding infection control policies, as environmental reservoirs play central roles in its spread. We reviewed experimental studies, environmental surveillance reports, and comparative disinfection efficacy data to summarise interactions between C. auris and commonly used biocidal classes: chlorine-based oxidizers, alcohol formulations, biguanides, and quaternary ammonium compounds. Mechanistic findings on biofilm formation, efflux activity, and stress-response pathways were integrated to contextualise tolerance behaviour. Evidence indicates C. auris shows reduced susceptibility to quaternary ammonium compounds and demonstrates variable, strain-dependent tolerance to alcohol-based disinfectants, particularly with organic load or suboptimal contact times. Chlorine-based oxidising agents maintain reliable activity at appropriate concentrations and exposure durations. Biofilm formation enhances environmental persistence and diminishes surface decontamination efficiency. C. auris requires disinfectant strategies distinct from other Candida species. Effective infection prevention depends on optimised agent selection, adequate contact times, and consideration of surface and organic-matter conditions. Tailored decontamination protocols are essential to limit environmental persistence and interrupt nosocomial transmission.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70169"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13000866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147481273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Performance of Sōna GM LFA in Pulmonary Infections Caused by Different Aspergillus Species.","authors":"Manuela Loaiza-Oliva, Paula Funes, María Mercedes Romero, María Sol Barbagelata, Soledad Gamarra, Guillermo Garcia-Effron","doi":"10.1111/myc.70155","DOIUrl":"10.1111/myc.70155","url":null,"abstract":"<p><strong>Background: </strong>Although Aspergillus fumigatus remains the primary cause of invasive aspergillosis, other species-including emerging cryptic ones-are increasingly identified in clinical settings. These species often exhibit distinct antifungal susceptibility profiles, emphasising the importance of species-level identification for effective patient management. Galactomannan, once considered specific to A. fumigatus, is now widely used for aspergillosis diagnosis regardless of species. However, its performance in detecting non-fumigatus infections remains insufficiently characterised.</p><p><strong>Objective: </strong>To evaluate the diagnostic performance of the Sōna GM LFA (IMMY) in BAL samples from patients with pulmonary infections attributed to different Aspergillus spp., including cryptic species.</p><p><strong>Methods: </strong>We evaluated the diagnostic performance of the Sōna galactomannan LFA (IMMY) in bronchoalveolar lavage samples from 49 patients (15% paediatric) with probable invasive aspergillosis or COVID-associated pulmonary aspergillosis, confirmed by microscopy and culture.</p><p><strong>Results: </strong>The etiologic agents comprised A. fumigatus sensu stricto (46.9%), A. terreus ss. (16.3%), A. flavus ss. (16.3%), A. niger ss. (6.1%), A. nidulans (4.1%) and five cryptic species (10.2%). Galactomannan index values varied across species and clinical group, with particularly low readings (0.09-0.31) in six cases (five involving cryptic species), suggesting reduced assay sensitivity for cryptic taxa. Paediatric samples also showed lower galactomannan levels compared to adults.</p><p><strong>Conclusions: </strong>This study highlights the limitations of galactomannan assays in detecting cryptic Aspergillus infections, showing consistently low galactomannan levels in BAL. Multicenter studies are needed to validate findings and refine clinical algorithms.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70155"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13003193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evolving Landscape of Dermatophytosis in the UAE: Trichophyton indotineae and Related Genotypes.","authors":"Hari Pankaj Vanam, Akela Ghazawi, Dana Aljneibi, Mushtaq Khan, Fouzia Jabeen, Ahmed R Alsuwaidi, Jens Thomsen, Mohammad AlBataineh, Stefan Weber, Connie Gibas, Nathan P Wiederhold, Fatima Al Dhaheri","doi":"10.1111/myc.70167","DOIUrl":"10.1111/myc.70167","url":null,"abstract":"<p><strong>Background: </strong>Dermatophytosis remains one of the most prevalent superficial fungal infections worldwide, increasingly complicated by the emergence of terbinafine-resistant Trichophyton indotineae. Despite its global spread, molecular and clinical data from the United Arab Emirates (UAE) remain limited.</p><p><strong>Objectives: </strong>We conducted the first comprehensive molecular and phenotypic characterization of dermatophyte infections in the UAE to define the local epidemiology, antifungal resistance patterns and clinical outcomes.</p><p><strong>Methods: </strong>Between September 2024 and March 2025, 33 consecutive clinical dermatophyte isolates associated with tinea infections were collected through passive laboratory-based surveillance across the Abu Dhabi Emirate. Isolates underwent macroscopic and microscopic examination, urease testing, internal transcribed spacer (ITS) genotyping for species identification and sequencing of the squalene epoxidase (SQLE) gene to detect mutations associated with terbinafine resistance. Phylogenetic analyses were conducted using reference ITS genotypes. Antifungal susceptibility testing was conducted on 28 isolates following CLSI M38 guidelines.</p><p><strong>Results: </strong>Among the 33 isolates, 26 (78.8%) were confirmed as T. indotineae, with SQLE mutations detected in 88.5% of isolates, most frequently Phe397Leu and Ala448Thr. Terbinafine demonstrated high non-wild-type rates (63.6%; MIC₅₀/MIC₉₀: 2/> 2 μg/mL), while itraconazole and griseofulvin retained activity. Significant inter-laboratory discordance was observed (6.1% agreement), with conventional methods failing to detect any T. indotineae cases. Most patients (84.8%) reported no travel history, indicating autochthonous transmission. Clinical outcomes were poor despite prolonged combination therapy: only 30.8% achieved improvement while 69.2% experienced recurrence.</p><p><strong>Conclusions: </strong>This study provides baseline evidence of endemic terbinafine-resistant T. indotineae in the UAE, underscoring urgent needs for molecular diagnostics, routine susceptibility testing and antifungal stewardship programs to guide effective management strategies.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70167"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147499436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Epidemiological Analyses of 12,976 Culture-Positive Superficial Fungal Infections in Shanghai, East China.","authors":"Chunjiao Zheng, Wenjing He, Jingwen Tan, Xiaoping Liu, Yuanyuan Wang, Lulu Li, Linan Ni, Qian Yu, Lianjuan Yang","doi":"10.1111/myc.70164","DOIUrl":"10.1111/myc.70164","url":null,"abstract":"<p><strong>Background: </strong>Superficial fungal infections (SFIs) are common dermatological conditions, and both domestic and international reports indicate an increasing incidence in recent years. Their prevalence is strongly affected by climate, temperature, humidity and lifestyle, resulting in significant regional variations.</p><p><strong>Objective: </strong>To describe the epidemiological characteristics of superficial fungal infections in Shanghai, East China, from 2022 to 2024.</p><p><strong>Methods: </strong>This single-centre retrospective observational study was conducted at Shanghai Skin Disease Hospital. Clinical specimens, including skin scales, hair and nails, were examined using KOH microscopy, followed by culture on Sabouraud dextrose agar. Fungal identification was primarily based on colony morphology and microscopic features.</p><p><strong>Results: </strong>A total of 12,976 cases of superficial fungal infections were recorded during the study period. Onychomycosis was the most common infection (33.75%), followed by tinea cruris (20.05%) and tinea pedis (18.63%). Significant gender differences were identified: females showed higher rates of onychomycosis and tinea pedis, while males more frequently presented with tinea cruris, tinea corporis and other SFIs. The highest number of infections occurred in individuals aged 31-40 years, with onychomycosis, tinea pedis and tinea versicolor being most prevalent in this group. Among culture-positive samples, dermatophytes accounted for the majority (52.54%), with Trichophyton rubrum being the most frequently isolated species (44.27%). Dermatophyte infections were more common in males, whereas yeast infections were more frequent in females. Dermatophyte infections peaked in the 31-40 and 61-70 age groups, while yeast infections were most common among individuals aged 21-40.</p><p><strong>Conclusion: </strong>This study provides valuable epidemiological insights into superficial fungal infections in Shanghai, offering necessary guidance for clinical diagnosis, treatment decision-making and prevention strategies.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70164"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12958013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence Rates, Species Distribution and Antifungal Susceptibility of Rare Candida and Related Saccharomycotina Yeasts Causing Bloodstream Infections in 28 Medical Centres.","authors":"Carolina Palamin Buonafine, Alexander E Dos Santos, Larissa Molina Favarello, Regielly C R Cognialli, Valerio R Aquino, Ana V A Mendes, Thais Guimarães, Teresa C T Sukiennik, Fabianne Carlesse, Paulo de Tarso O E Castro, Vania A Vicente, Elaine C Francisco, Arnaldo L Colombo","doi":"10.1111/myc.70149","DOIUrl":"10.1111/myc.70149","url":null,"abstract":"<p><strong>Background: </strong>There is a lack of contemporary studies addressing the prevalence rates and antifungal susceptibility of bloodstream infections (BSI) caused by rare yeasts (RY) from the Saccharomycotina subphylum.</p><p><strong>Objectives: </strong>This 16-year multicentre study (2007-2023) aimed to assess the prevalence and antifungal susceptibility of rare yeasts isolated from BSIs in 28 Brazilian tertiary care hospitals.</p><p><strong>Methods: </strong>Yeasts from the Saccharomycotina subphylum, excluding common Candida species and Basidiomycota, were selected from BSI episodes. Species were identified using proteomics and molecular methods. Antifungal susceptibility testing was performed by EUCAST broth microdilution.</p><p><strong>Results: </strong>Among 2655 BSI episodes, 100 (3.76%) involved rare yeasts. Prevalence showed a slight, non-significant increase from 3.16% (2007-2015) to 4.17% (2016-2023; p = 0.183). A total of 11 genera and 20 species were identified, with Clavispora lusitaniae (20%), Wickerhamomyces anomalus (16%), Candida haemulonii (14%) and Candida duobushaemulonii (12%) being the most frequent species. Eight species, including Pichia kluyveri and Kodamaea ohmeri, appeared only in the latter period. Most rare yeasts showed low MICs to amphotericin B and anidulafungin, except for Clavispora lusitaniae, Candida haemulonii and Candida duobushaemulonii, which were less susceptible to amphotericin B. Wickerhamomyces anomalus and Candida duobushaemulonii also showed reduced susceptibility to fluconazole.</p><p><strong>Conclusions: </strong>This study provides valuable insights into the prevalence rates, species distribution and antifungal susceptibility of rare yeasts causing BSIs in tertiary care hospitals. Our findings highlight the need for continuous surveillance, incorporation of new diagnostic and tailored therapeutic strategies to mitigate morbidity and mortality due to invasive infections caused by emerging fungal pathogens.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 3","pages":"e70149"},"PeriodicalIF":3.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12955699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}