Utility of MALDI-ToF MS for Recognition and Antifungal Susceptibility of Nannizzia, an Underestimated Group of Dermatophytes.

IF 3.1 2区医学 Q1 DERMATOLOGY

Mycoses Pub Date : 2025-09-01 DOI:10.1111/myc.70117

Chao Tang, Xue Kong, Jasmijn Jansen, Katharina Vossgroene, Thi-Lam-An Vu, Boris Oberheitmann, Marlou Tehupeiory-Kooreman, Shaoqin Zhou, Xin Zhou, Clement Kin-Ming Tsui, Weida Liu, Yingqian Kang, Sarah A Ahmed, Sybren de Hoog

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引用次数: 0

Abstract

Background: Geophilic Nannizzia dermatophytes are increasingly implicated in stubborn skin, hair, and nail infections, yet MALDI-TOF MS evaluations and antifungal-susceptibility data have focused almost exclusively on N. gypsea. Biochemical profiles and MICs cut-offs are limited.

Objectives: To benchmark two commercial MALDI-TOF MS libraries and to determine in vitro activity of eight antifungals against a genus-wide panel of Nannizzia species.

Methods: One-hundred-and-three ITS-confirmed isolates representing 12 species were grown on potato-dextrose agar (PDA) for 7-14 days. Spectra were acquired with (i) the MSI-2 Dermatophyte Library after 4-14 days' PDA incubation (100 cultures) and (ii) the Bruker MALDI Biotyper Filamentous-Fungi Library 6.0/2023 after ≤ 3 days' growth in Sabouraud-dextrose broth (SDB) (73 cultures). BCCM/IHEM strains could not be evaluated on the Biotyper because of licence restrictions, leaving 73 non-duplicate isolates for direct MSI-2 vs MBT comparison. EUCAST E.Def 11.0 micro-broth dilution determined MICs for eight agents.

Results: MSI-2 achieved its highest accuracy with PDA day-7 cultures (45/73, 62%), whereas the liquid Biotyper protocol yielded 49/73 correct identifications (67%) within four days. Accepting low-confidence scores (≥ 1.7) from either library increased overall accuracy to 73%. MSI-2 remained superior for N. gypsea (73%) and uniquely detected N. nana (50%), which is absent from the current Biotyper release. Conversely, the Biotyper outperformed MSI-2 for N. incurvata, N. fulva, and N. praecox. Six very rare species (N. lorica, N. aenigmatica, N. corniculata, N. duboisii, N. perplicata, N. polymorpha) were not recognised by either database. Terbinafine displayed the lowest geometric mean MIC (0.009 mg/L); fluconazole and griseofulvin showed the highest values, and one US N. fulva isolate exhibited elevated itraconazole/voriconazole MICs (1 mg/L).

Conclusions: Diagnostic coverage of Nannizzia remains incomplete. Expanding commercial MALDI-ToF MS libraries with spectra from rare species and performing routine susceptibility testing are essential to optimise patient management.

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MALDI-ToF质谱对一种被低估的皮肤植物群南霉属的识别和抗真菌敏感性的应用。

背景：嗜土性Nannizzia皮肤真菌越来越多地与顽固的皮肤、头发和指甲感染有关，然而MALDI-TOF MS评估和抗真菌敏感性数据几乎只集中在N. gypsea上。生化特征和mic的切断是有限的。目的：对两个商业化的MALDI-TOF质谱文库进行比较，并确定8种抗真菌药物对南氏菌属的体外活性。方法：在马铃薯-葡萄糖琼脂（PDA）培养基上培养12种103株经its鉴定的分离株，培养7 ~ 14 d。使用(i) PDA培养4-14天后的MSI-2皮肤真菌文库（100个培养物）和（ii）在Sabouraud-dextrose肉液（SDB）中生长≤3天后（73个培养物）的Bruker MALDI Biotyper丝状真菌文库6.0/2023获得光谱。由于许可限制，BCCM/IHEM菌株无法在Biotyper上进行评估，留下73个非重复分离株用于直接MSI-2与MBT的比较。EUCAST E.Def 11.0微肉汤稀释法测定了8种药物的mic。结果：MSI-2在PDA第7天的培养中达到了最高的准确性（45/ 73.62%），而液体生物typer方案在4天内获得了49/73的正确鉴定（67%）。接受来自任一文库的低置信度评分（≥1.7）可将总体准确率提高到73%。MSI-2对gypsea N.（73%）和nana N.（50%）的检测仍有优势，而目前的Biotyper版本中没有。相反，Biotyper在无头稻、富力稻和早熟稻上的表现优于MSI-2。6种非常罕见的物种（N. lorica， N. aenigmatica， N. corniculata， N. duboisii， N. perplicata， N. polymorpha）均未被数据库识别。特比萘芬的几何平均MIC最低（0.009 mg/L）；氟康唑和灰黄霉素的mic值最高，一株US N. fulva分离物的伊曲康唑/伏立康唑mic值升高（1 mg/L）。结论：nannizia的诊断覆盖率仍然不完整。扩大商用MALDI-ToF质谱库与稀有物种的光谱和执行常规药敏试验是优化患者管理的必要条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycoses 医学-皮肤病学

CiteScore

10.00

自引率

8.20%

发文量

143

审稿时长

6-12 weeks

期刊介绍： The journal Mycoses provides an international forum for original papers in English on the pathogenesis, diagnosis, therapy, prophylaxis, and epidemiology of fungal infectious diseases in humans as well as on the biology of pathogenic fungi. Medical mycology as part of medical microbiology is advancing rapidly. Effective therapeutic strategies are already available in chemotherapy and are being further developed. Their application requires reliable laboratory diagnostic techniques, which, in turn, result from mycological basic research. Opportunistic mycoses vary greatly in their clinical and pathological symptoms, because the underlying disease of a patient at risk decisively determines their symptomatology and progress. The journal Mycoses is therefore of interest to scientists in fundamental mycological research, mycological laboratory diagnosticians and clinicians interested in fungal infections.