Mycoses最新文献

{"title":"Impact of Concomitant Metamizole Treatment on the Exposure of Mould-Active Triazoles.","authors":"Dorian Vanneste, Ine De Booser, Kristina Nadrah, Matej Somrak, Matthias Gijsen, Aleš Matos, Katja Kalan, Bart Morlion, Steffen Rex, Saba Battelino, Katrien Lagrou, Johan Maertens, Isabel Spriet","doi":"10.1111/myc.70173","DOIUrl":"10.1111/myc.70173","url":null,"abstract":"<p><strong>Aim: </strong>Metamizole is an analgesic drug with moderate cytochrome P450 (CYP) inductive properties. The antifungal triazoles are metabolized by several CYP enzymes, but the interaction with metamizole remains poorly described. We investigated the influence of metamizole on the exposure of voriconazole, isavuconazole, and posaconazole.</p><p><strong>Methods: </strong>Patients from UZ Leuven (Belgium) and Ljubljana University Medical Centre (Slovenia) receiving voriconazole, isavuconazole, or posaconazole concomitantly with metamizole were included in the study. Routine therapeutic drug monitoring (TDM) measurements collected between January 2019 and December 2024 were retrieved retrospectively. TDM concentrations outside of concomitant therapy were collected as controls. The influence of metamizole and other clinically relevant covariates was analysed using generalized estimating equations (GEE).</p><p><strong>Results: </strong>A total of 126 distinct treatments with a triazole from 115 patients, accounting for 392 measurements, were included in the study. GEE analysis revealed a significant negative association between the 7-day cumulative metamizole dose and lower voriconazole and posaconazole concentrations. Additionally, C-reactive protein had a positive association with voriconazole concentrations. Only ICU admission and patient characteristics, that is, sex and weight, had a significant influence on isavuconazole concentrations.</p><p><strong>Conclusion: </strong>Concomitant therapy with metamizole led to lower voriconazole and posaconazole concentrations, presumably through induction of CYP enzymes and possibly UDP-glucuronyltransferase. We recommend avoiding concomitant use of metamizole with the antifungal triazoles to prevent underexposure and treatment failure or frequent TDM if the combination cannot be avoided. Further studies are needed to confirm our findings and investigate the influence of metamizole on other triazoles.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70173"},"PeriodicalIF":3.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13175134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dermoscopic Features of Tinea Capitis in Korean Patients: Correlation With Clinical Subtypes, Age Groups and Causative Dermatophytes.","authors":"Thi Quynh Trang Tran, Gwangil Kim, Hyun-Ji Ryu, Su-Kyung Park, Kyung-Hwa Nam, Seok-Kweon Yun, HyoHyun Yoo, Jin Park","doi":"10.1111/myc.70184","DOIUrl":"10.1111/myc.70184","url":null,"abstract":"<p><strong>Background: </strong>Tinea capitis (TC) presents with diverse clinical manifestations that may complicate diagnosis. Dermoscopy is a useful adjunctive tool, but data correlating dermoscopic findings with clinical and mycological characteristics in Korean patients remain limited.</p><p><strong>Objective: </strong>To characterize dermoscopic features of TC in Korean patients and evaluate their associations with clinical subtypes, age distributions and causative dermatophytes.</p><p><strong>Methods: </strong>We retrospectively analysed 71 patients with mycologically confirmed TC diagnosed between 2011 and 2025. Dermoscopic patterns were compared across clinical subtypes, fungal species and age groups. Representative dystrophic hairs were further examined using super-resolution confocal laser scanning microscopy.</p><p><strong>Results: </strong>The cohort demonstrated a bimodal age distribution, with peaks in children (≤ 10 years) and elderly adults (≥ 61 years). Microsporum canis was the predominant pathogen (71.8%). The most frequent dermoscopic findings were scaling (83.1%), black dots (78.9%), follicular hyperkeratosis (78.9%) and broken hairs (78.9%). Empty follicles were significantly associated with Trichophyton infections (p = 0.001), whereas zigzag hairs, Morse code-like hairs, bent hairs and scaling were significantly more frequent in Microsporum infections (p < 0.05). Comma hairs were commonly observed in diffuse pustular and black dot subtypes. Children more often exhibited Morse code-like hairs, whereas elderly patients showed higher rates of empty follicles and perifollicular erythema. Confocal microscopy confirmed distinct structural shaft damage corresponding to characteristic dermoscopic patterns.</p><p><strong>Conclusion: </strong>Dermoscopy provides valuable diagnostic and aetiological clues in TC, enabling differentiation between clinical subtypes and prediction of causative fungal species. These findings are especially relevant in adult-predominant, Microsporum-endemic settings and support dermoscopy as an important adjunct to routine clinical evaluation.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70184"},"PeriodicalIF":3.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13182684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147964156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Patterns of Osteoarticular, Bone Marrow and Urogenital Paracoccidioidomycosis.","authors":"Wdson Luis Lima Kruschewsky, Mariane Cappellini Stricagnolo, Mônica Scarpelli Martinelli Vidal, Gil Benard, Vítor Falcão de Oliveira, Tiago Alexandre Cocio, Adriana Satie Gonçalves Kono Magri, Marcello Mihailenko Chaves Magri","doi":"10.1111/myc.70182","DOIUrl":"10.1111/myc.70182","url":null,"abstract":"<p><strong>Background: </strong>Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America, classically characterized by pulmonary, mucosal and lymphatic involvement. Extrapulmonary manifestations affecting uncommon organs remain poorly characterized and may contribute to diagnostic delay and adverse outcomes.</p><p><strong>Methods: </strong>We conducted a retrospective case series of proven or probable atypical extrapulmonary PCM diagnosed at a Brazilian tertiary referral center between 2006 and 2025. A systematic review registered in PROSPERO and conducted according to PRISMA recommendations identified osteoarticular, bone marrow and urogenital PCM cases between 1970 to November 2025 in PubMed/MEDLINE and Embase without language restrictions.</p><p><strong>Results: </strong>Among 205 PCM patients evaluated at our institution, 14 (6.8%) presented atypical extrapulmonary involvement. Combined with 153 cases identified in 75 studies, 167 cases were analysed. Osteoarticular involvement was the most frequent manifestation (53.9%), predominantly affecting young patients with acute/subacute disease and presenting mainly as osteomyelitis with lytic lesions. Bone marrow involvement occurred exclusively in acute/subacute PCM and was associated with post-mortem diagnosis (9.7%) and markedly elevated mortality (77.8%). Urogenital PCM mainly affected middle-aged men with chronic disease, frequently involving the testicles and prostate, and was associated with delayed diagnosis, surgical intervention (32.7%), and mortality (32.0%). Distinct epidemiological profiles, clinical forms and outcomes were consistently observed across atypical sites.</p><p><strong>Conclusion: </strong>Atypical extrapulmonary PCM manifestations follow recognizable clinical patterns associated with diagnostic delay and poor outcomes, supporting earlier investigation and timely antifungal therapy in endemic settings.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70182"},"PeriodicalIF":3.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive Fungal Infections After Intestine Transplantation: Epidemiology and Outcomes.","authors":"Mayyadah H Alabdely, Jessica Lum, Blanca E Gonzalez, Masato Fujiki, Zachary A Yetmar","doi":"10.1111/myc.70180","DOIUrl":"10.1111/myc.70180","url":null,"abstract":"<p><strong>Background: </strong>Invasive fungal infections (IFIs) represent a major complication after intestine transplantation, with reported incidence rates between 40% and 49%. These infections are associated with high morbidity and allograft loss. This study evaluates the impact of post-transplant IFIs on graft outcomes in intestine transplant recipients.</p><p><strong>Methods: </strong>We conducted a retrospective cohort study of 152 patients who underwent intestine transplantation from 2008 to 2022. The primary outcome was IFI, defined as proven or probable by EORTC/MSGERC criteria. The secondary outcome was a composite of allograft failure or death. Analyses were conducted using multivariable Cox proportional hazards models, with non-baseline variables incorporated as time-dependent exposures.</p><p><strong>Results: </strong>Among 152 intestine transplant recipients, 56 (36.8%) developed post-transplant IFI. Median time to IFI was 83.5 days (IQR 19.5-444.2), with Candida infections occurring early and non-Candida infections occurring later. Candida species accounted for 73% of IFIs, most commonly C. glabrata, C. albicans, and C. parapsilosis, with 29.3% showing fluconazole resistance. Most infections were intra-abdominal and bloodstream. Redo transplantation (HR 2.44, 95% CI 1.20-4.95; p = 0.014), anastomotic leak (HR 2.23, 95% CI 1.02-4.90; p = 0.045), and augmented immunosuppression (HR 4.73, 95% CI 1.94-11.53; p < 0.001) were independent predictors of Candida IFI. IFI was associated with a markedly increased risk of allograft loss or death (HR 3.67, 95% CI 2.30-5.83; p < 0.001).</p><p><strong>Conclusions: </strong>Post-transplant IFIs are common and associated with allograft loss or mortality in intestine transplant recipients. Early recognition and aggressive management of IFIs remain critical to improving transplant outcomes.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 5","pages":"e70180"},"PeriodicalIF":3.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of Mucormycosis: Current Situation, Challenges and Future Prospects.","authors":"Yixuan Qu, Yuling Xiao, Lina Liu, Junyan Qu","doi":"10.1111/myc.70175","DOIUrl":"10.1111/myc.70175","url":null,"abstract":"<p><p>Mucormycosis, an aggressive fungal infection caused by members of the order Mucorales, progresses rapidly and is associated with high mortality, particularly in immunocompromised hosts such as patients with uncontrolled diabetes, transplant recipients or those with COVID-19-associated immunosuppression. Early diagnosis remains challenging with current clinical methods, yet it is essential to reduce mortality. This review examines the evolution of diagnostic strategies for mucormycosis, ranging from conventional techniques such as histopathology, culture and microscopy to advanced and emerging methods including molecular assays, serological testing, imaging and metabolomics. We also explore the ongoing transition towards integrated, rapid and non-invasive diagnostic platforms that leverage novel biomarkers, portable devices and artificial intelligence. These new technologies have the potential to facilitate early diagnosis, thereby enabling early treatment and reducing the mortality rate of mucormycosis.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70175"},"PeriodicalIF":3.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T. indotineae: A New Emergent Fungal Pathogen Driven by Global Travel.","authors":"Bryan Ortiz, Florent Morio, Kateryn Aguilar, Franklin García, Gustavo Fontecha, Alicia Moreno-Sabater","doi":"10.1111/myc.70170","DOIUrl":"10.1111/myc.70170","url":null,"abstract":"<p><strong>Background: </strong>The global spread of Trichophyton indotineae, a multidrug-resistant dermatophyte, remains insufficiently understood due to limited data on migration-associated dissemination and emerging local transmission. This study reviewed available evidence and integrated international migration statistics to assess the worldwide distribution of this pathogen and the influence of human mobility on its expansion.</p><p><strong>Methods: </strong>A systematic search in Scopus and PubMed (September 9, 2025) included epidemiological studies, case reports, and letters. Extracted variables comprised country of diagnosis, probable country of infection, and reported transmission scenarios. Migration data from the International Organization for Migration were incorporated to explore links between population movements and fungal spread, including indirect or undocumented routes.</p><p><strong>Results: </strong>Of the 224 publications identified, 89 met the inclusion criteria, reporting a total of 1215 cases outside India across 36 countries. Europe accounted for 545 cases, followed by North America with 90 cases and Oceania with 87 cases, while South America and Africa reported only sporadic detections. Fifty studies described imported infections, whereas 11 documented autochthonous transmission. Integrated analysis revealed three primary dissemination corridors: intra-Asian, Asia-Europe, and Asia-America.</p><p><strong>Conclusions: </strong>Trichophyton indotineae is now globally disseminated and strongly shaped by travel and migration. Coordinated international action, standardized diagnostics, harmonized treatment guidelines, and integrated surveillance are urgently required to mitigate its expanding public health impact worldwide.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70170"},"PeriodicalIF":3.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13033971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Epidemiology and Infection Control of Candida Auris in Shanghai.","authors":"Mingjing Wei, Jian Guo, Li Li, Junhao Zhu, Yuyuan Xue, Guo Chen, Fan Yang, Wenjuan Wu, Min Zhu","doi":"10.1111/myc.70176","DOIUrl":"10.1111/myc.70176","url":null,"abstract":"<p><strong>Background: </strong>Candida auris is a globally emerging multidrug-resistant fungus. Its dissemination is driven by patient movement and asymptomatic carriers. However, molecular epidemiological studies linking imported cases to local strains in large metropolitan areas remain limited.</p><p><strong>Objectives: </strong>To analyse the molecular epidemiology and resistance mechanisms of 33 clinical isolates in Shanghai and propose an integrated infection control strategy tailored to metropolitan healthcare settings.</p><p><strong>Methods: </strong>Isolates from 19 patients were identified via MALDI-TOF MS and sequencing. Antifungal susceptibility and biofilm formation were assessed. Environmental contamination was evaluated using PCR. Whole-genome sequencing (WGS) was employed to identify resistance mutations and perform phylogenetic analysis.</p><p><strong>Results: </strong>All 33 isolates were fluconazole-resistant. Resistance to amphotericin B and echinocandins was observed in 9 and 4 isolates, respectively. All echinocandin-resistant strains harboured the Fks1-S639F mutation. Phylogenetic analysis revealed that Clade III (South African clade) was the dominant lineage. Colonisation was concentrated in the urinary tract and inguinal regions, while bedside tables were identified as key environmental reservoirs. All strains exhibited strong biofilm-forming capacity.</p><p><strong>Conclusions: </strong>ICU patients serve as primary reservoirs, with transmission driven by interregional movement. The distinct colonisation patterns and environmental hotspots provide specific targets for screening. Integrating PCR-based environmental monitoring with culture methods constitutes an effective strategy. These findings underscore the need for a standardised protocol integrating molecular diagnostics to optimise infection control in urban hospitals.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70176"},"PeriodicalIF":3.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Hyphal Suspension Approach for MIC Testing of Mould Fungi.","authors":"Hailin Zheng, Dongmei Li, Naicen Ge, Huan Mei, Xiaofang Li, Weida Liu","doi":"10.1111/myc.70177","DOIUrl":"10.1111/myc.70177","url":null,"abstract":"<p><strong>Background: </strong>Filamentous fungi cause a wide range of superficial and invasive infections, and antifungal susceptibility assay is essential for guiding effective therapy. Standard broth microdilution methods rely on microconidia-based inocula; however, many clinically important moulds produce insufficient microconidia due to intrinsic characteristics or culture conditions, limiting the applicability of conventional testing and often necessitating empirical treatment.</p><p><strong>Objectives: </strong>To overcome this limitation, we developed an alternative method using precisely quantified mycelial cell suspensions for MIC determination.</p><p><strong>Methods: </strong>The method was evaluated using 115 fungal strains and compared with traditional microconidia-based testing.</p><p><strong>Results: </strong>High concordance was observed between the two approaches, with agreement rates of 90% or higher across three common dermatophytes causing tinea infections and three representative fungi responsible for invasive infections.</p><p><strong>Conclusions: </strong>This mycelial-based approach provides a reliable and practical alternative for antifungal susceptibility testing of poorly sporulating moulds and may improve the efficiency and accessibility of MIC testing in clinical laboratories.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70177"},"PeriodicalIF":3.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence Screening of Chronic Aspergillus Infection in a Post-Tuberculosis Cohort in Senegal: A Cross-Sectional Study Comparing ELISA and Rapid Diagnostic Tests.","authors":"Touré Mariama, Ndiaye Ibrahima Mbaye, Sow Djiby, Diallo Mamadou Alpha, Diongue Khadim, Seck Mame Cheikh, Ndiaye Mouhamadou, Ndiaye Daouda, Ndiaye Faly Diop, Fortes Louise, Denning David, Badiane Aida Sadikh","doi":"10.1111/myc.70174","DOIUrl":"10.1111/myc.70174","url":null,"abstract":"<p><strong>Background: </strong>Chronic pulmonary aspergillosis (CPA) is a significant, yet often overlooked, sequela of pulmonary tuberculosis (TB), particularly in resource-limited settings. Data on the seroprevalence of Aspergillus infection in Senegal is absent, and diagnostic capacity is limited. This study aimed to determine the seroprevalence of Aspergillus-specific antibodies among patients with a history of TB in Senegal and to evaluate the performance of a Rapid Diagnostic Test (RDT) against an Enzyme-Linked Immunosorbent Assay (ELISA).</p><p><strong>Methods: </strong>A cross-sectional study was conducted between April 30 to August 31, 2025 at two health centers in Senegal: Wakhinane and Yeumbeul among patients presenting with chronic respiratory symptoms. After obtaining informed consent from adult participants or from parents/guardians for minors, a blood sample was collected from each participant and tested for Aspergillus antibodies using both a rapid diagnostic test (RDT) and an ELISA. Results were classified as positive, negative, invalid, or not tested. Prevalence and agreement between the two assays were calculated using valid results only. Demographic data were collected, and descriptive statistics with test performance analyses were conducted.</p><p><strong>Results: </strong>The overall seroprevalence was significantly higher by ELISA (11.9%; 38/320) than by RDT (5.5%; 11/201). Site-specific analysis revealed disparities: Wakhinane showed higher RDT positivity (4.5% vs. 1.0%), while Yeumbeul had higher ELISA positivity (13.5% vs. 11.2%). The cohort was predominantly male (66.0%) with a median age of 30 years. ROC analysis of the ELISA identified an optimal threshold that balanced sensitivity (78%) and specificity (89%).</p><p><strong>Conclusion: </strong>This study provided the first serological evidence of substantial Aspergillus antibody prevalence among post-TB patients in Senegal, suggesting a significant burden of undiagnosed CPA. The higher sensitivity of ELISA makes it essential for surveillance and confirmation, while the RDT's practicality offers a viable option for initial screening in peripheral clinics. These findings underscored the urgent need to integrate CPA diagnosis into routine post-TB care and to strengthen fungal diagnostic capacity in West Africa.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70174"},"PeriodicalIF":3.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13069597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147662844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and Antifungal Susceptibility of Malassezia spp. Isolated From Brazilian Patients With Pityriasis Versicolor and Seborrheic Dermatitis.","authors":"Diogo Coelho de Pádua Oliveira, Ana Paula Possa, Ana Raquel de Oliveira Santos, Ana Kleiber P Borges, Patrícia Silva Cisalpino, Raquel Vilela, Carlos Augusto Rosa, Susana Johann","doi":"10.1111/myc.70171","DOIUrl":"10.1111/myc.70171","url":null,"abstract":"<p><strong>Background: </strong>Malassezia spp. are part of the microbiota of many animals, including humans. However, under certain conditions, they can become pathogenic. Diseases associated with Malassezia include pityriasis versicolor (PV), seborrheic dermatitis (SD), Malassezia folliculitis, atopic dermatitis, psoriasis and fungemia.</p><p><strong>Objective: </strong>The present study aimed to describe the distribution of Malassezia species among Brazilian patients with PV and SD and to evaluate their susceptibility profiles to common antifungals.</p><p><strong>Methods: </strong>In this study, 102 clinical samples from patients with PV or SD were analysed. Clinical isolates of Malassezia were identified at the species level by sequencing the D1/D2 variable domains of the large subunit rRNA gene. Antifungal susceptibility was assessed using a modified microbroth dilution method adapted for the growth of Malassezia species.</p><p><strong>Results: </strong>Among the 40 cultures obtained, six Malassezia species were identified. M. furfur was the most prevalent species (40.0%), followed by M. sympodialis (27.5%), M. globosa (15.0%), M. japonica (7.5%) and both M. yamatoensis and M. slooffiae (5.0% each). All isolates exhibited high MICs to caspofungin (> s16 μg/mL) and to isoconazole (MIC₅₀ = 8 μg/mL). Miconazole and clotrimazole showed MIC₅₀ values of 4 μg/mL, while itraconazole and ketoconazole were more active, with an MIC₅₀ of 0.125 μg/mL.</p><p><strong>Conclusion: </strong>This study showed the diversity of Malassezia species causing PV and SD in Brazil, including the rare species M. yamatoensis and M. japonica. These findings highlight the importance of antifungal susceptibility testing for these species to guide appropriate therapy.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"69 4","pages":"e70171"},"PeriodicalIF":3.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13032048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}