Mycoscience最新文献_第7页

Coprinopsis cinerea Cla4 protein kinase is required for the assembly of the hyphal tip apparatus that enables the rapid linear growth of apical cells. cl4蛋白激酶是使顶细胞快速线形生长的菌丝尖端装置的组装所必需的。

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.005

Tetsuya Kakizaki, Koh Sato, Keishi Osakabe, Hajime Muraguchi

{"title":"Coprinopsis cinerea Cla4 protein kinase is required for the assembly of the hyphal tip apparatus that enables the rapid linear growth of apical cells.","authors":"Tetsuya Kakizaki, Koh Sato, Keishi Osakabe, Hajime Muraguchi","doi":"10.47371/mycosci.2024.10.005","DOIUrl":"10.47371/mycosci.2024.10.005","url":null,"abstract":"The C. cinerea septins are observed at the hyphal tip and septum in the growing vegetative hyphae. In Saccharomyces cerevisiae, septin dynamics have been suggested to be regulated by septin phosphorylation through protein kinases such as Cla4. To gain insight into the relationship between C. cinerea septins and C. cinerea Cla4 protein kinase (Cc.Cla4), we conducted disruption of the C. cinerea cla4 gene (Cc.cla4) by CRISPR/Cas9 system and analyzed the phenotypes of Cc.cla4Δ strains. The vegetative hyphae of the Cc.cla4Δ strains grow slowly in the zigzag shape and are highly branched compared to the wild-type hyphae. The enhanced green fluorescent protein (EGFP) tagged Cc.Cdc3 septin in the Cc.cla4Δ strain was observed in the growing vegetative hyphae. Although EGFP-Cc.Cdc3 signals localized at the plasma membrane of the hyphal tip region, they did not accumulate as a dome with a hole at the hyphal tip as observed in the wild type. Instead, the Cc.Cdc3 was found to stay at the hyphal tip, which split into two tips thereafter. These results suggest that Cc.Cla4 is required for assembly of the hyphal tip apparatus that enables the rapid linear growth of the apical cells.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"307-316"},"PeriodicalIF":1.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term effects of forest management on the dynamics of Tricholoma matsutake harvest over 41 years in a Pinus densiflora forest in Nagano Prefecture, Japan. 日本长野县松林41年森林管理对松茸收获动态的长期影响

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.09.003

Hitoshi Furukawa, Koudai Tokuoka, Aira Mizuno, Kazuhiro Katagiri, Kazuhiko Masuno, Yujiro Tanaka, Shinjiro Oya, Akiyoshi Yamada

{"title":"Long-term effects of forest management on the dynamics of Tricholoma matsutake harvest over 41 years in a Pinus densiflora forest in Nagano Prefecture, Japan.","authors":"Hitoshi Furukawa, Koudai Tokuoka, Aira Mizuno, Kazuhiro Katagiri, Kazuhiko Masuno, Yujiro Tanaka, Shinjiro Oya, Akiyoshi Yamada","doi":"10.47371/mycosci.2024.09.003","DOIUrl":"10.47371/mycosci.2024.09.003","url":null,"abstract":"Tricholoma matsutake is an important wild edible mycorrhizal mushroom in Japan. The domestic matsutake harvest has been declining for 80 y. Nagano Prefecture, a central region of Japan, is the area that has been most productive of matsutake during the past 20 y. To study how matsutake harvesting was affected by forest management, an experimental site was established in a pine forest in Nagano Prefecture in 1980. Two plots were established: a managed plot (MP) in which all trees except pine were cut, pine trees were thinned, and soil litter layer was removed; and a control plot (CP) with no forest management. The numbers of matsutake fruiting bodies were recorded annually in both plots for 41 y. The annual matsutake harvest was stable in the MP but decreased in the CP, while the number of shiro increased in the MP and was stable at the low level in the CP. These trends were suggested to be affected significantly by the thicker humus layer and large root biomass other than pines in the CP. This is the longest record of matsutake harvest in the managed forest, which reveals the importance of such long-term forest management for the sustainable matsutake harvesting.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"298-306"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sclerotinia borealis and its varieties, var. subarctica var. nov. and var. okhotskana, var. nov. 北核菌及其变种，亚北极11月变种和11月变种okhotskana。

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.002

Izumi Saito, Oleg B Tkachenko, Shiro Kuninaga, Naoyuki Matsumoto, Tamotsu Hoshino

引用次数: 0

Two new Tricholoma species in the sect. Genuina from pine forests in Japan. 标题日本松林真属口蘑二新种。

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.09.001

Wataru Aoki, Masaki Fukuda, Akiyoshi Yamada

{"title":"Two new Tricholoma species in the sect. Genuina from pine forests in Japan.","authors":"Wataru Aoki, Masaki Fukuda, Akiyoshi Yamada","doi":"10.47371/mycosci.2024.09.001","DOIUrl":"10.47371/mycosci.2024.09.001","url":null,"abstract":"\"Matsushimeji\" identified as Tricholoma albobrunneum belonging to sect. Genuina is an edible mushroom commonly used in Japan. This species has been suggested to include another cryptic species with common morphological characteristics and identical internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nuc rDNA). We compared T. albobrunneum specimens sampled in Japan and Denmark, including the holotype. Phylogenetic analyses of nuc rDNA ITS and intergenic spacer 1 (IGS1) regions, and tef-1a and rpb2 genes identified two phylogroups in Japanese T. albobrunneum. In addition, both Japanese phylogroups of T. albobrunneum were distinguished from European T. albobrunneum. Concatenated phylogenetic tree analysis based on these four DNA regions also distinguished two Japanese clades within T. albobrunneum. Here, we report two new species, T. matsushimeji distributed on Honshu Island under two-needle pines and T. miyama-matsushimeji on Rishiri Island under a five-needle pine from Japan.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"278-287"},"PeriodicalIF":1.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient PCR - based Approach for Rapid Identification of Earth Star Mushrooms Employing Species-Specific Primers. 利用种特异性引物快速鉴定土星菇的高效PCR方法。

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-10-11 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.09.002

Tharnrat Kaewgrajang, Leela Nakpong, Yatawee Foongchomchoi, Chatchai Ngernsaengsaruay, Runchida Khunkrai, Kantida Bunlerlerd, Sasitorn Hasin, Itsarapong Voraphab, Baramee Sakolrak, Penpitcha Choosa-Nga, Cherdchai Phosri, Warong Suksavate, Mingkwan Nipitwattanaphon

{"title":"Efficient PCR - based Approach for Rapid Identification of Earth Star Mushrooms Employing Species-Specific Primers.","authors":"Tharnrat Kaewgrajang, Leela Nakpong, Yatawee Foongchomchoi, Chatchai Ngernsaengsaruay, Runchida Khunkrai, Kantida Bunlerlerd, Sasitorn Hasin, Itsarapong Voraphab, Baramee Sakolrak, Penpitcha Choosa-Nga, Cherdchai Phosri, Warong Suksavate, Mingkwan Nipitwattanaphon","doi":"10.47371/mycosci.2024.09.002","DOIUrl":"10.47371/mycosci.2024.09.002","url":null,"abstract":"Astraeus species are valuable edible ectomycorrhizal mushrooms, particularly in Asia. The partial hypogenous nature of Astraeus fruit bodies in soil present challenges for sample collection and studying species distribution. In this study, we developed a PCR-based approach for identifying Astraeus. Two universal primers (AUPF1/AUPR1 and AUPF3/AUPR3) and specific primers for A. asiaticus (AAF4/AAR4), A. odoratus (AOF4/AOR4), and A. sirindhorniae (ASF2/ASR4) were designed based on the alignment of internal transcribed spacer sequences from various Astraeus species. Primer verification was performed by generating amplicons from extracted DNA of Astraeus fruit bodies and 130 soil samples collected from beneath various host plants of Astraeus spp. These novel primers were efficient and precise in identifying Astraeus species. Our results have implications for multi-sample assays for Astraeus identification and investigations into species distribution through large-scale inventories.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"288-297"},"PeriodicalIF":1.4,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reintroducing Akanthomyces ampullifer: providing genetic barcodes, culture, and updated description for the dipteran pathogen rediscovered in Germany. 重新引入壶腹赤色菌：提供在德国重新发现的双翅目病原体的遗传条形码、培养和更新描述。

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-10-10 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.08.001

Alena Kubátová, Bernd Kunz, Vít Hubka

{"title":"Reintroducing Akanthomyces ampullifer: providing genetic barcodes, culture, and updated description for the dipteran pathogen rediscovered in Germany.","authors":"Alena Kubátová, Bernd Kunz, Vít Hubka","doi":"10.47371/mycosci.2024.08.001","DOIUrl":"10.47371/mycosci.2024.08.001","url":null,"abstract":"The genus Akanthomyces (Ascomycota, Hypocreales) includes entomopathogenic species known to infect a variety of insects and spiders. In this study, we present the first isolate of A. ampullifer characterized by molecular methods, found on dead bodies of the common cave limoniid Limonia nubeculosa (Diptera) in the subterranean spaces of southwestern Germany. In total, seven specimens exhibited distinctive morphological traits that, when compared with historical records, confirm their identification as A. ampullifer-particularly noted for its affinity to dipteran hosts. Absent from culture collections and molecular repositories, this species has eluded detailed scientific documentation using modern methods. Our research bridges this knowledge gap, providing the first genetic identification barcodes of five genes, living culture, cultivation requirements, and an updated description. This overlooked fungus is phylogenetically most closely related to the species A. pyralidarum, A. laosensis, and some other species mostly associated with adult moths. It demonstrates a unique morphological signature with monoblastic phialides forming a layer on the surface of synnemata and produces long, cylindrical, chain-forming conidia. It prefers lower temperatures, exhibiting an inability to grow at 25 °C, coupled with notably slow growth in culture.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"260-269"},"PeriodicalIF":1.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular phylogeny, morphology, mycorrhizal symbiosis, and putative distribution of the arbuscular mycorrhizal fungus Epigeocarpum japonicum (Glomeraceae). 丛枝菌根真菌Epigeocarpum japonicum （Glomeraceae）的分子系统发育、形态、菌根共生和推测分布。

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-10-09 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.08.002

Masahide Yamato, Muneyuki Ohmae, Takamichi Orihara, Ryota Kusakabe, Bruno Tomio Goto, Janusz Błaszkowski

{"title":"Molecular phylogeny, morphology, mycorrhizal symbiosis, and putative distribution of the arbuscular mycorrhizal fungus Epigeocarpum japonicum (Glomeraceae).","authors":"Masahide Yamato, Muneyuki Ohmae, Takamichi Orihara, Ryota Kusakabe, Bruno Tomio Goto, Janusz Błaszkowski","doi":"10.47371/mycosci.2024.08.002","DOIUrl":"10.47371/mycosci.2024.08.002","url":null,"abstract":"We collected in Japan five sporocarpic specimens morphologically identical to those of Epigeocarpum japonicum, a recently described Glomeromycota species. Although 18S-ITS-28S nuc rDNA sequences obtained from these sporocarps showed high sequence variability, phylogenetic analyses based on 18S-ITS-28S, the largest subunit of the RNA polymerase II (rpb1) gene, and concatenated sequences of the two loci convincingly demonstrated the identity of these sporocarps to E. japonicum. Importantly, the 18S-ITS-28S+rpb1 analyses highlighted the key role of rpb1 sequences in reconstructing the phylogenies of Glomeromycota taxa with strongly divergent rDNA sequences. Upon inoculation with sporocarpic spores, E. japonicum formed mycorrhiza with arbuscules and vesicles, which was not confirmed in the original description of the species. Comparisons of E. japonicum 18S sequences with Glomeromycota DNA sequences available in a public database indicated that E. japonicum is a cosmopolitan species and is mainly associated with plants in natural habitats such as grasslands, shrublands, and forests. Phylogenetic analyses also confirmed the autonomy of E. crypticum, another known species of the genus Epigeocarpum whose sporocarps were originally found in Brazil.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"270-277"},"PeriodicalIF":1.4,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spencermartinsiella japonica f.a., sp. nov., a novel yeast species isolated from biofilm in a reverse osmosis system. 反渗透系统中从生物膜中分离的一种新型酵母。

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-09-20 eCollection Date: 2024-01-01 DOI: 10.47371/mycosci.2024.05.004

Hiroyasu Doi, Ayaka Mishima, Ryuichiro Ikeda

引用次数: 0

Gene expression analysis for stem browning in the mushroom Lentinula edodes 香菇茎干褐变的基因表达分析

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-08-31 DOI: 10.47371/mycosci.2024.07.003

Jili Zhang, Yuki Tanaka, Akiko Ono, Takumi Sato, Toshiyuki Suzuki, Saya Akimoto, Yuki Tanaka, Shoko Iwami, Aya Iwamoto, Norio Tanaka, Naotake Konno, Tomohiro Suzuki

{"title":"Gene expression analysis for stem browning in the mushroom Lentinula edodes","authors":"Jili Zhang, Yuki Tanaka, Akiko Ono, Takumi Sato, Toshiyuki Suzuki, Saya Akimoto, Yuki Tanaka, Shoko Iwami, Aya Iwamoto, Norio Tanaka, Naotake Konno, Tomohiro Suzuki","doi":"10.47371/mycosci.2024.07.003","DOIUrl":"https://doi.org/10.47371/mycosci.2024.07.003","url":null,"abstract":"The mushroom Lentinula edodes, is consumed worldwide and has high industrial value because of its rich content of bioactive compounds such as ergothioneine and eritadenine. Currently, mainstream artificial cultivation methods for this mushroom typically use synthetic logs. However, browning of the stem’s interior (stem browning) has been observed during the cultivation in some L. edodes strains. Although browning does not affect the taste or other qualities of the mushroom, it gives consumers a perception of “poor quality”, and is a major challenge for producers. To identify the genes responsible for stem browning in this mushroom, we performed differential gene expression analysis during stem browning development and quantified it using real-time PCR. Our results indicated that certain oxidoreductases, such as tyrosinase and laccase, were significantly upregulated during the progression of stem browning. The results obtained in the present study provide valuable insights to address the problem of stem browning in mushroom L. edodes.\u0000","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"6 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological responses of two Aspergillus oryzae strains to various metal ions at different concentrations 两种黑曲霉菌株对不同浓度的各种金属离子的形态学反应

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-08-23 DOI: 10.47371/mycosci.2024.04.001

Liyun Liu, Kanae Sakai, Takumi Tanaka, Ken-Ichi Kusumoto

{"title":"Morphological responses of two Aspergillus oryzae strains to various metal ions at different concentrations","authors":"Liyun Liu, Kanae Sakai, Takumi Tanaka, Ken-Ichi Kusumoto","doi":"10.47371/mycosci.2024.04.001","DOIUrl":"https://doi.org/10.47371/mycosci.2024.04.001","url":null,"abstract":"Aspergillus species take up various metal ions from environment. The morphology of Aspergillus oryzae strains can vary under the influence of various metal ions. Here, the effects of Ti4+, V3+, Sr2+, Ba2+, Al3+, Fe2+, Zn2+, Mn2+, Ca2+, and Cu2+ on morphological parameters of A. oryzae strains RIB40 and RIB143 were estimated. Colony diameter, conidiation, vesicle head size, and stipe width in both strains varied with concentration. Ti4+, Sr2+, Ba2+, Al3+, Fe2+, and Ca2+ affected conidiation in similar tendency between two strains. The effects of Ti4+, V3+, Sr2+, and Ba2+ on the morphology of A. oryzae are reported here for the first time. Induction of growth of both strains by 0.0001% Ti4+ may help the fermentation industry. Induction of conidiation in RIB40 by 0.001% Cu2+ confirmed previous results that low concentrations of Cu2+ promote the growth of Aspergillus. The most novel finding is that 0.001% Zn2+ increased the vesicle head size in RIB40; possible reasons are discussed.\u0000","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"7 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142227727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0