MycosciencePub Date : 2022-01-01DOI: 10.47371/mycosci.2022.08.002
Taiga Kasuya, Kentaro Hosaka, Makoto Kakishima
{"title":"<i>Puccinia caricis-smilacis</i>, a new caricicolous rust fungus producing spermogonial and aecial stages on <i>Smilax china</i> in Japan.","authors":"Taiga Kasuya, Kentaro Hosaka, Makoto Kakishima","doi":"10.47371/mycosci.2022.08.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.08.002","url":null,"abstract":"<p><p><i>Puccinia caricis-smilacis</i>, a new species of caricicolous rust fungus was described based on morphological characteristics and nuclear rDNA sequences from Japan. The heteroecious life cycle of <i>P. caricis-smilacis</i> was elucidated via field observations, inoculation experiments and molecular analyses. This rust fungus produces uredinial and telial stages on <i>Carex fibrillosa,</i> and spermogonial and aecial stages on <i>Smilax china</i>.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 5","pages":"235-241"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/82/4f/MYC-63-235.PMC10024967.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physiological characteristics of pure cultures of a white-colored truffle <i>Tuber japonicum</i>.","authors":"Shota Nakano, Akihiko Kinoshita, Keisuke Obase, Noritaka Nakamura, Hitomi Furusawa, Kyotaro Noguchi, Takashi Yamanaka","doi":"10.47371/mycosci.2022.01.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.01.002","url":null,"abstract":"<p><p>A white-colored truffle <i>Tuber japonicum</i>, indigenous to Japan, is an ascomycetous ectomycorrhizal fungus. To clarify the physiological characteristics of this fungus, we investigated the influence of culture medium, temperature, and sources of nitrogen (N) and carbon (C) on the growth of five strains. <i>Tuber japonicum</i> strains grew better on malt extract and modified Melin-Norkrans medium, and showed peak growth at 20 °C or 25 °C. This fungus utilized inorganic (NH<sub>4</sub> <sup>+</sup> and NO<sub>3</sub> <sup>-</sup>) and organic N sources (casamino acids, glutamine, peptone, urea, and yeast extract). Additionally, this fungus utilized various C sources, such as monosaccharide (arabinose, fructose, galactose, glucose, and mannose), disaccharide (maltose, sucrose, and trehalose), polysaccharide (dextrin and soluble starch), and sugar alcohol (mannitol). However, nutrient sources that promote growth and their effects on growth promotion widely varied among strains. This can result from the strain difference in enzyme activities involved in the assimilation and metabolism of these sources.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 2","pages":"53-57"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b5/e3/MYC-63-053.PMC9999085.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9388787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mushroom poisoning in Thailand between 2003 and 2017.","authors":"Sayanh Somrithipol, Umpawa Pinruan, Sujinda Sommai, Phongsawat Khamsuntorn, Janet Jennifer Luangsa-Ard","doi":"10.47371/mycosci.2022.08.003","DOIUrl":"https://doi.org/10.47371/mycosci.2022.08.003","url":null,"abstract":"<p><p>The frequent occurrence of mushroom poisoning cases in the wet season in Thailand has long been recognized but has never been quantitatively analyzed. This study aims to analyze mushroom poisoning cases in Thailand between 2003 and 2017 and focused on their association with the rainfall. The results revealed 22,571 cases and 106 deaths in this period. Cases were higher for females than males, adults than children, and agriculturists than people in other occupations. Cases were higher in the northeastern and northern regions than in the central, south, and east regions. There are strong effects of seasonality on mushroom poisoning cases. Over the 15-y period, 17,337 cases occurred in the wet season (May-Sep) compared with 5,234 cases in the dry season (Oct-Apr). The number of monthly poisoning cases were high in men, mature adults, agriculturists, people living in the rural areas and people living in the Northeastern and Northern provinces. Moreover, a strong positive correlation was shown between the number of monthly poisoning cases and the amount of monthly rainfall.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 6","pages":"267-273"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/60/49/MYC-63-267.PMC10042301.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9740635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Novel acid trehalase belonging to glycoside hydrolase family 37 from <i>Pleurotus</i> sp.: cloning, expression and characterization.","authors":"Gaku Tsutsumi, Chikako Kuroki, Kengo Kamei, Mizuho Kusuda, Masami Nakazawa, Tatsuji Sakamoto, Mariko Ishikawa, Shinji Harada, Hitoshi Kobayashi, Kenji Ouchi, Satoshi Inatomi, Minoru Sakaguchi, Takeo Iwamoto, Mitsuhiro Ueda","doi":"10.47371/mycosci.2022.09.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.09.001","url":null,"abstract":"<p><p>The N-terminal amino acid sequence of the <i>Pleurotus</i> sp. 90 kDa protein was in good agreement with the corresponding sequence of the glycoside hydrolase (GH) family 37 protein (trehalase) from <i>P. ostreatus</i> PC 15 v2.0. The length of the <i>Pleurotus</i> sp. trehalase gene was 2247 bp, encoding a protein of 749 amino acids with a predicted molecular mass of 81.2 kDa. The molecular mass of the recombinant enzyme was estimated to be about 117 kDa by SDS-PAGE. We found that the recombinant enzyme comprised an <i>N</i>-glycosylated sugar chain and that its optimum pH and temperature were 4.5 and 40 ºC, respectively. Moreover, this enzyme exhibited high activity against trehalose exclusively. We found that the enzyme is novel acid trehalase belonging to GH family 37.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 6","pages":"284-292"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/2b/MYC-63-284.PMC10024966.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9740636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2022-01-01DOI: 10.47371/mycosci.2021.10.004
Qin Na, Zewei Liu, Hui Zeng, Xianhao Cheng, Yupeng Ge
{"title":"<i>Crepidotus yuanchui</i> sp. nov. and <i>C. caspari</i> found in subalpine areas of China.","authors":"Qin Na, Zewei Liu, Hui Zeng, Xianhao Cheng, Yupeng Ge","doi":"10.47371/mycosci.2021.10.004","DOIUrl":"https://doi.org/10.47371/mycosci.2021.10.004","url":null,"abstract":"<p><p>Only a few <i>Crepidotus</i> species have been previously reported from subalpine areas of China. Members of <i>Crepidotus</i> possessing an orange-yellow pileus are rare in China as well. Here, we describe <i>Crepidotus yuanchui</i>, a new species characterized by an orange-yellow pileus, ovoid basidiospores, and abundant cylindric to narrowly utriform cheilocystidia. This species is widely distributed above 2000 m asl in Yunnan Province. In addition, <i>C. caspari</i>, which is a distinct species based on its combined characteristics of smooth basidiospores and clamp connections, is newly recorded and detailed from subalpine China. The results of phylogenetic analyses of ITS + nLSU sequences based on maximum likelihood and Bayesian inference methods support the recognition of these two species. Photographs, descriptions, line drawings, and comparisons of related species are provided.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 1","pages":"1-11"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/d9/MYC-63-001.PMC10043943.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9742126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Tuber torulosum</i>: A new truffle species decorated with moniliform cystidia from Japan.","authors":"Akihiko Kinoshita, Kohei Yamamoto, Toshiyuki Tainaka, Toshifumi Handa, Akiyoshi Yamada","doi":"10.47371/mycosci.2021.10.005","DOIUrl":"https://doi.org/10.47371/mycosci.2021.10.005","url":null,"abstract":"<p><p>We describe a new truffle species, <i>Tuber torulosum</i>, based on molecular and morphological analyses. This species forms a single globose ascospore per ascus, pale yellow in color, as do Japanese <i>T. flavidosporum</i> and Chinese <i>T. turmericum</i> and <i>T. xanthomonosporum</i> in the Japonicum clade of the <i>Tuber</i> phylogeny. However, it can be distinguished from them microscopically by its whitish tomentose mycelium that partially covers the ascoma surface and the mesh size of its spore ornamentation. Cystidia are moniliform and yellowish to reddish. Molecular phylogenetic analysis using the internal transcribed spacer and partial large subunit regions of ribosomal DNA also supports <i>T. torulosum</i> as a distinct species. On the basis of our results, we provide a key to species in the Japonicum clade.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 1","pages":"26-32"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/b7/MYC-63-026.PMC10045829.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9742131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2022-01-01DOI: 10.47371/mycosci.2022.02.002
Jun-Hong Dong, Ya-Xing Wu, Chang-Lin Zhao
{"title":"Two new species of <i>Steccherinum</i> (<i>Polyporales</i>, <i>Basidiomycota</i>) from southern China based on morphology and DNA sequence data.","authors":"Jun-Hong Dong, Ya-Xing Wu, Chang-Lin Zhao","doi":"10.47371/mycosci.2022.02.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.02.002","url":null,"abstract":"<p><p>Two new wood-inhabiting fungal species, <i>Steccherinum hirsutum</i> and <i>S. yunnanense</i> spp. nov., are proposed based on a combination of morphological features and molecular evidence. Sequences of internal transcribed spacer and large subunit region of nuculear ribosomal RNA gene of the studied samples were generated, and phylogenetic analyses were performed using maximum likelihood, maximum parsimony and bayesian inference methods. <i>Steccherinum hirsutum</i> is characterized by an annual growth habit, stipitate basidiocarps with scarlet to red, odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae negative in Melzer's reaction, and acyanophilous basidiospores measuring 2.5-3.5 × 1.5-2.5 µm. <i>Steccherinum yunnanense</i> is distinguished by resupinate basidiomata with odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae, strongly encrusted cystidia and ellipsoid, hyaline, thin-walled, smooth basidiospores (3.5-4.5 × 2-3.5 µm). The phylogenetic analyses comfirmed that two new species nest in <i>Steccherinum</i>, in the residual polyporoid clade.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 2","pages":"65-72"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/8e/MYC-63-065.PMC10024968.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9382384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2022-01-01DOI: 10.47371/mycosci.2022.05.003
Yuri K Novozhilov, Ilya S Prikhodko, Nadezhda A Fedorova, Oleg N Shchepin, Vladimir I Gmoshinskiy, Martin Schnittler
{"title":"<i>Lamproderma vietnamense</i>: a new species of myxomycetes with reticulate spores from Phia Oắc - Phia Đén National Park (northern Vietnam) supported by molecular phylogeny and morphological analysis.","authors":"Yuri K Novozhilov, Ilya S Prikhodko, Nadezhda A Fedorova, Oleg N Shchepin, Vladimir I Gmoshinskiy, Martin Schnittler","doi":"10.47371/mycosci.2022.05.003","DOIUrl":"https://doi.org/10.47371/mycosci.2022.05.003","url":null,"abstract":"<p><p>A new species of <i>Lamproderma</i> (<i>Myxomycetes</i>), described herein as <i>L. vietnamense</i>, was recovered in the field on ground litter from mountain subtropical forests (Phia Oắc - Phia Đén National Park) of northern Vietnam. Morphological details were examined by light and scanning electron microscopy. The species is characterized by a distinct and unique combination of morphological features, including a bright blue, shiny and very thin membranous peridium, a small dome-shaped columella, rigid, straight, branched, brown capillitial threads which gradually become pale at the periphery and finally colorless at the tips and small-meshed, banded-reticulate spores with 9-12 meshes across the spore diameter and solid walls without perforations 0.3-0.5 µm high. The stability of the taxonomic characters of <i>L. vietnamense</i> is supported by two well-developed collections found in 2018 and 2019. Partial sequences of three molecular markers (SSU, EF1α, COI) for both collections are identical. A two-gene phylogeny of the first two markers displays the two known accessions as a well-separated entity and indicates affinity of the new species with <i>L. columbinum</i> (the type taxon of the genus), <i>L. violaceum</i>, and several nivicolous <i>Lamproderma</i> species.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 4","pages":"149-155"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/52/a7/MYC-63-149.PMC10043568.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The study of early screening technique for fruiting ability of <i>Lentinula edodes</i> hybrid progenies.","authors":"Yu Li, Hailong Yu, Liangmin Li, Chunyan Song, Xiaodong Shang, Qi Tan, Lujun Zhang, Qiaozhen Li","doi":"10.47371/mycosci.2022.06.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.06.001","url":null,"abstract":"<p><p>Crossbreeding is the most commonly used method in breeding of <i>Lentinula edodes</i>, however low fruiting rate of the hybrids has always caused troubles and barriers for breeders. An early screening method of the fruiting ability could make the breeding work more efficient. In this paper, a rapid and high-throughput laccase activity detection method based on agar diffusion principle was developed. In this way, we investigated the constitutive and inducible extracellular laccase activity of 36 strains in a breeding population of <i>L. edodes</i> on different media and performed a correlation analysis with fruiting ability of these strains. The results showed the laccase activity of mycelium cultured in non-induced medium for 8 d could be used as an early screening index to judge whether it had fruiting ability at the later stage. Early rapid and simple screening method for hybrid populations was established based on laccase activity characteristics of mycelia. 127 strains from another 5 different hybrid populations were used to verify the early screening method. From the validation results, the early screening method was effective, but the appropriate screening threshold was needed to select according to the cross population, which would greatly to improve the breeding efficiency of <i>L. edodes</i>.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 5","pages":"189-196"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/07/4e/MYC-63-189.PMC10012344.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9756152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>In-vitro</i> symbiotic germination of seeds of five mycoheterotrophic <i>Gastrodia</i> orchids with <i>Mycena</i> and <i>Marasmiaceae</i> fungi.","authors":"Michiru Kitahara, Kosuke Nagamune, Akihiko Kinoshita, Chigusa Yugeta, Naoto Ohara, Akane Shimazaki, Yumi Yamashita, Tomohisa Yukawa, Naoki Endo, Yuki Ogura-Tsujita","doi":"10.47371/mycosci.2022.04.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.04.001","url":null,"abstract":"<p><p>We performed <i>in-vitro</i> germination tests on seeds from five <i>Gastrodia</i> orchids (<i>G. confusa</i>, <i>G. elata</i> var. <i>elata</i>, <i>G. elata</i> var. <i>pallens</i>, <i>G. nipponica</i>, and <i>G. pubilabiata</i>) using one <i>Marasmiaceae</i> and two <i>Mycena</i> isolates. <i>Mycena</i> sp. 1 promoted germination of all five <i>Gastrodia</i> orchids, with root and/or tuber formation observed in <i>G. confusa</i>, <i>G. nipponica</i>, and <i>G. pubilabiata</i>. No additional growth was observed in the other two orchids. <i>Mycena</i> sp. 2 induced <i>G. confusa</i>, <i>G. elata</i> var. <i>elata</i>, and <i>G. nipponica</i> germination, whereas <i>Marasmiaceae</i> sp. 1 induced <i>G. nipponica</i> and <i>G. pubilabiata</i> germination. Phylogenetic analyses indicated that the two <i>Mycena</i> isolates represent distinct lineages within the <i>Mycenaceae</i>. <i>Mycena</i> sp. 1 and <i>Marasmiaceae</i> sp. 1 are closely related to <i>Mycena abramsii</i> and <i>Marasmiellus rhizomorphogenus</i>, respectively. Our results imply that <i>Mycena</i> and marasmioid fungi play important roles in early development in <i>Gastrodia</i> species, and that <i>Mycena</i> fungi in particular may be common mycobionts of <i>Gastrodia</i> species. Root and/or tuber development was observed with four plant-fungus combinations, implying that these associations persist throughout the life cycle, whereas <i>G. elata</i> var. <i>elata</i> may require different associates over time. Our findings will contribute to elucidating the mycorrhizal associations of mycoheterotrophic orchids throughout their life cycle.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 3","pages":"88-95"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/78/MYC-63-088.PMC10042302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9755631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}