Mycoscience最新文献_第8页

The study of early screening technique for fruiting ability of Lentinula edodes hybrid progenies. 香菇杂交后代结实能力的早期筛选技术研究。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.06.001

Yu Li, Hailong Yu, Liangmin Li, Chunyan Song, Xiaodong Shang, Qi Tan, Lujun Zhang, Qiaozhen Li

{"title":"The study of early screening technique for fruiting ability of Lentinula edodes hybrid progenies.","authors":"Yu Li, Hailong Yu, Liangmin Li, Chunyan Song, Xiaodong Shang, Qi Tan, Lujun Zhang, Qiaozhen Li","doi":"10.47371/mycosci.2022.06.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.06.001","url":null,"abstract":"Crossbreeding is the most commonly used method in breeding of Lentinula edodes, however low fruiting rate of the hybrids has always caused troubles and barriers for breeders. An early screening method of the fruiting ability could make the breeding work more efficient. In this paper, a rapid and high-throughput laccase activity detection method based on agar diffusion principle was developed. In this way, we investigated the constitutive and inducible extracellular laccase activity of 36 strains in a breeding population of L. edodes on different media and performed a correlation analysis with fruiting ability of these strains. The results showed the laccase activity of mycelium cultured in non-induced medium for 8 d could be used as an early screening index to judge whether it had fruiting ability at the later stage. Early rapid and simple screening method for hybrid populations was established based on laccase activity characteristics of mycelia. 127 strains from another 5 different hybrid populations were used to verify the early screening method. From the validation results, the early screening method was effective, but the appropriate screening threshold was needed to select according to the cross population, which would greatly to improve the breeding efficiency of L. edodes.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 5","pages":"189-196"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/07/4e/MYC-63-189.PMC10012344.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9756152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro symbiotic germination of seeds of five mycoheterotrophic Gastrodia orchids with Mycena and Marasmiaceae fungi. 五种真菌异养天麻兰科植物种子与迈锡纳科和马拉斯科真菌的体外共生萌发。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.04.001

Michiru Kitahara, Kosuke Nagamune, Akihiko Kinoshita, Chigusa Yugeta, Naoto Ohara, Akane Shimazaki, Yumi Yamashita, Tomohisa Yukawa, Naoki Endo, Yuki Ogura-Tsujita

{"title":"In-vitro symbiotic germination of seeds of five mycoheterotrophic Gastrodia orchids with Mycena and Marasmiaceae fungi.","authors":"Michiru Kitahara, Kosuke Nagamune, Akihiko Kinoshita, Chigusa Yugeta, Naoto Ohara, Akane Shimazaki, Yumi Yamashita, Tomohisa Yukawa, Naoki Endo, Yuki Ogura-Tsujita","doi":"10.47371/mycosci.2022.04.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.04.001","url":null,"abstract":"We performed in-vitro germination tests on seeds from five Gastrodia orchids (G. confusa, G. elata var. elata, G. elata var. pallens, G. nipponica, and G. pubilabiata) using one Marasmiaceae and two Mycena isolates. Mycena sp. 1 promoted germination of all five Gastrodia orchids, with root and/or tuber formation observed in G. confusa, G. nipponica, and G. pubilabiata. No additional growth was observed in the other two orchids. Mycena sp. 2 induced G. confusa, G. elata var. elata, and G. nipponica germination, whereas Marasmiaceae sp. 1 induced G. nipponica and G. pubilabiata germination. Phylogenetic analyses indicated that the two Mycena isolates represent distinct lineages within the Mycenaceae. Mycena sp. 1 and Marasmiaceae sp. 1 are closely related to Mycena abramsii and Marasmiellus rhizomorphogenus, respectively. Our results imply that Mycena and marasmioid fungi play important roles in early development in Gastrodia species, and that Mycena fungi in particular may be common mycobionts of Gastrodia species. Root and/or tuber development was observed with four plant-fungus combinations, implying that these associations persist throughout the life cycle, whereas G. elata var. elata may require different associates over time. Our findings will contribute to elucidating the mycorrhizal associations of mycoheterotrophic orchids throughout their life cycle.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 3","pages":"88-95"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/78/MYC-63-088.PMC10042302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9755631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Japanese "nameko" mushrooms (Pholiota microspora) produced via sawdust-based cultivation exhibit severe genetic bottleneck associated with a single founder. 日本“nameko”蘑菇(微孢子菌)通过木屑为基础的栽培表现出严重的遗传瓶颈与单一的创始人。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.03.002

Akira S Hirao, Atsushi Kumata, Toshihito Takagi, Yoshito Sasaki, Takashi Shigihara, Eiichi Kimura, Shingo Kaneko

{"title":"Japanese \"nameko\" mushrooms (Pholiota microspora) produced via sawdust-based cultivation exhibit severe genetic bottleneck associated with a single founder.","authors":"Akira S Hirao, Atsushi Kumata, Toshihito Takagi, Yoshito Sasaki, Takashi Shigihara, Eiichi Kimura, Shingo Kaneko","doi":"10.47371/mycosci.2022.03.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.03.002","url":null,"abstract":"Pholiota microspora (\"nameko\" in Japanese) is one of the most common edible mushrooms, especially in Japan, where sawdust-based cultivation is the most dominant method accounting for 99% of the production. The current strains for sawdust cultivation in Japan are considered to have been derived from a single wild strain collected from Fukushima, Japan, implying that commercial nameko mushrooms are derived from a severe genetic bottleneck. We tested this single founder hypothesis by developing 14 microsatellite markers for P. microspora to evaluate the genetic diversity of 50 cultivars and 73 wild strains isolated from across Japan. Microsatellite analysis demonstrated that sawdust-cultivated strains from Japan were significantly less genetically diverse than the wild strains, and the former displayed a significant bottleneck signature. Analyzing the genetic relationships among all genotypes also revealed that the sawdust-cultivated samples clustered into one monophyletic subgroup. Moreover, the sawdust-cultivated samples in Japan were more closely related than full-sibs. These results were consistent with the single founder hypothesis that suggests that all commercial nameko mushrooms produced in Japan are descendants of a single ancestor. Therefore, we conclude that cultivated P. microspora originated from a single domestication event that substantially reduced the diversity of commercial nameko mushrooms in Japan.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 3","pages":"79-87"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/61/e9/MYC-63-079.PMC10012341.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lentinula ixodes comb. nov. (Omphalotaceae, Agaricales) including new records in Brazil. 香菇。11 .在巴西发现的新记录(鞘翅目，鞘翅目)。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.08.001

Jadson J S Oliveira, Tiara S Cabral, Ruby Vargas-Isla, José F B Silva, Doriane P Rodrigues, Nelson Menolli, Mariana P Drewinski, Noemia K Ishikawa

{"title":"Lentinula ixodes comb. nov. (Omphalotaceae, Agaricales) including new records in Brazil.","authors":"Jadson J S Oliveira, Tiara S Cabral, Ruby Vargas-Isla, José F B Silva, Doriane P Rodrigues, Nelson Menolli, Mariana P Drewinski, Noemia K Ishikawa","doi":"10.47371/mycosci.2022.08.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.08.001","url":null,"abstract":"In the search for new strains of edible mushrooms in the Brazilian Amazon Forest, we found Lentinula specimens different from Lentinula raphanica. These were described morphologically and evaluated phylogenetically within the Lentinula clade. The mating system was determined, and interbreeding compatibility with L. raphanica was verified. The basidiomata have a cinnamon or deep orange to fulvous brown, moist to dry pileus, occasionally with whitish scales; crowded whitish cream lamellae; and an eccentric to lateral stipe. The typical, predominant basidiospores are 4.4-7.2 µm in length. Endogenous, elongate (7.8-14 µm) basidiospores were also found in some specimens. The long spores seem rare and occasional, but nonetheless a novelty for the group. Basidia are homogeneous in size, cheilocystidia are pyriform or bulboid, and caulocystidia are long and spheropedunculate. The hyphae of the pileipellis are pigment-encrusted. The characteristics match those of Agaricus ixodes originally described from Guyana, currently a synonym of Lentinula boryana. In the phylogenetic trees, such taxon appears distinct from L. boryana and sister to L. raphanica with strong support. This unique lineage was confirmed to be reproductively isolated from sympatric L. raphanica strains. Lentinula ixodes comb. nov. is the second species of the genus reported in the Amazon Forest.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 6","pages":"254-266"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0e/a7/MYC-63-254.PMC10032364.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9740632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A new edible species of Gomphus (Gomphaceae) from southwestern China. 标题中国西南贡麻属一新种。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.09.002

Jian-Wei Liu, Thatsanee Luangharn, Shan-Ping Wan, Ran Wang, Fu-Qiang Yu

引用次数: 2

Morchella nipponensis sp. nov. (Ascomycota, Pezizales): a paleoendemic species of section Morchella discovered in Japan. 日本羊肚菌sp. 11 .(子囊菌科，Pezizales):在日本发现的羊肚菌科古特有种。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.08.005

Philippe Clowez, Takumi Izumi, Paul-Bill Lamiable, Koichi Shibakusa, Camelia Minculeasa, Pablo Alvarado

引用次数: 0

Taxonomic revision of Termitomyces species found in Ryukyu Archipelago, Japan, based on phylogenetic analyses with three loci. 基于三个基因座系统发育分析的日本琉球群岛白蚁属物种分类修正。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2021.11.001

Yuuki Kobayashi, Miyuki Katsuren, Masaru Hojo, Shohei Wada, Yoshie Terashima, Masayoshi Kawaguchi, Gaku Tokuda, Kazuhiko Kinjo, Shuji Shigenobu

{"title":"Taxonomic revision of Termitomyces species found in Ryukyu Archipelago, Japan, based on phylogenetic analyses with three loci.","authors":"Yuuki Kobayashi, Miyuki Katsuren, Masaru Hojo, Shohei Wada, Yoshie Terashima, Masayoshi Kawaguchi, Gaku Tokuda, Kazuhiko Kinjo, Shuji Shigenobu","doi":"10.47371/mycosci.2021.11.001","DOIUrl":"https://doi.org/10.47371/mycosci.2021.11.001","url":null,"abstract":"Fungi in the genus Termitomyces are external symbionts of fungus-growing termites. The three rhizogenic Termitomyces species T. eurrhizus, T. clypeatus, and T. intermedius, and one species similar to T. microcarpus that lacks pseudorrhiza, have been reported from Ryukyu Archipelago, Japan. In contrast, only two genetic groups (types A and B) of Termitomyces vegetative mycelia have been detected in nests of the fungus-growing termite Odontotermes formosanus. In this study, we investigated the relationships between the mycelial genetic groups and the basidiomata of Termitomyces samples from the Ryukyu Archipelago. We found that all the basidioma specimens and the type B mycelia formed one clade that we identified as T. intermedius. Another clade consisted of the type A mycelia, which showed similarity to T. microcarpus, was identified as T. fragilis. Our results indicate that the Japanese T. eurrhizus and T. clypeatus specimens should re-named as T. intermedius.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 1","pages":"33-38"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/bd/MYC-63-033.PMC10045831.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9757351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Erysiphe ruyongzhengiana sp. nov., a new powdery mildew species on Aristolochia debilis, belonging to the Erysiphe aquilegiae clade. 儒永祯菌(Erysiphe ruyongzhenana sp. nov.)，马兜铃属马兜铃门白粉病一新种。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.05.005

Li Liu, Lin-Chong Hui, Shou-Rong Yu, Yu Li, Shu-Yan Liu

引用次数: 1

Morpho-anatomical and molecular characterization of a native mycorrhizal Amanita species associated with Guapira opposita (Nyctaginaceae) in the brazilian Atlantic Forest. 巴西大西洋森林中一种原生菌根毒伞属植物的形态解剖和分子特征。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.02.001

Ariadne Nóbrega Marinho Furtado, Ornella Comandini, Marco Leonardi, Andrea C Rinaldi, Maria Alice Neves

引用次数: 2

Association between corticolous myxomycetes and tree vitality in Cryptomeria japonica. 柳杉皮质黏菌与树木活力的关系。

IF 1.4 4区生物学

Mycoscience Pub Date : 2022-01-01 DOI: 10.47371/mycosci.2022.01.001

Kazunari Takahashi, Yu Fukasawa

{"title":"Association between corticolous myxomycetes and tree vitality in Cryptomeria japonica.","authors":"Kazunari Takahashi, Yu Fukasawa","doi":"10.47371/mycosci.2022.01.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.01.001","url":null,"abstract":"The bark of live trees provides an important microhabitat for corticolous myxomycetes. However, the association between the presence of myxomycetes and health of host trees has not been studied in detail. In this study, we aimed to investigate the relationship between tree vitality and myxomycetes on the bark of Cryptomeria japonica trees in a montane forest in western Japan. The vitality of trees was categorized into four grades based on the visual assessment of tree shape and leaf density in the upper branches. Myxomycetes on the bark surface were examined using the moist chamber culture method. A decline in tree vitality increased bark pH and decreased electrical conductivity of the bark exudates. Seventeen myxomycete species were recorded in 74 C. japonica trees. The structure of myxomycete communities varied between healthy and unhealthy trees, and species diversity increased as the vitality declined. The relative abundance of Cribraria confusa decreased as the vitality declined, while that of Paradiacheopsis solitaria increased. The results showed that acidophilic myxomycetes grew on healthy C. japonica bark, but changes in bark pH associated with vitality decline led to the weakening of acidity and shifted the community structure; thus, corticolous myxomycete diversity was enhanced as tree vitality decline.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 2","pages":"45-52"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/21/d7/MYC-63-045.PMC9999084.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9382388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1