IF 1.5 4区生物学

Mycoscience Pub Date : 2024-12-26 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.008

Tsutomu Hattori, Mitsuteru Akiba, Yuko Ota, Ahmad Mohd-Farid, Bee-Kin Thi, Su-See Lee

{"title":"Taxonomic reevaluation on Pyrrhoderma (Basidiomycota, Hymenochaetales) and reclassification of Pyrrhoderma species based on phylogenetic and type studies.","authors":"Tsutomu Hattori, Mitsuteru Akiba, Yuko Ota, Ahmad Mohd-Farid, Bee-Kin Thi, Su-See Lee","doi":"10.47371/mycosci.2024.10.008","DOIUrl":"10.47371/mycosci.2024.10.008","url":null,"abstract":"We reevaluated the species belonging to the genus Pyrrhoderma that were collected from Japan and Malaysia based phylogenetic analyses and morphological investigations. We found that Pyr. sendaiense is distinct from Pyr. adamantinum. Pyrrhoderma species with setal hyphae include Pyr. lamaoense, Pyr. noxium, Pyr. sublamaensis, and Pyr. williamsii. Pyrrhoderma noxium, synonymized with Pyr. sublamaense in earlier studies, was distinct from Pyr. sublamaense. All isolates from trees damaged by brown root rot were accommodated in the same clade with Pyr. noxium, and the cause of the disease was confirmed as Pyr. noxium. Pyrrhoderma williamsii resembles Pyr. lamaoense but is distinguished by a glabrous pileus surface and abundant hymenial setae. Fulvoderma species are similar to some Pyrrhoderma species but are distinguished by the duplex context separated by a thin crust composed of nonagglutinated hyphae. Some Inonotus species produce setal hyphae in the trama and context like some Pyrrhoderma species. However, the hyphae of these Inonotus species are acuminate at the tip, tapered toward the base, and often swollen toward the tip, whereas those of the Pyrrhoderma species are cylindrical and mostly equal. The following new combinations are proposed: Pyrrhoderma williamsii and Fulvoderma vallatum.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"66 1","pages":"88-108"},"PeriodicalIF":1.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root-associated ectomycorrhizal fungal communities in and around aggregated retention patches left in logged areas of Abies sachalinensis planted forests. 萨沙林冷杉人工林采伐后残留斑块内及其周围与根相关的外生菌根真菌群落

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-12-06 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.11.002

Keisuke Obase, Satoshi Yamanaka, Kenichi Ozaki

引用次数: 0

Host preference, life cycle, and classification of species of Ochropsora, including species formerly classified in Aplopsora and Cerotelium pro parte (Pucciniales). 寄主偏好、生活期及物种分类，包括原属applopsora和Cerotelium pro parte （puccininiales）的物种。

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-12-06 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.001

Yoshitaka Ono

{"title":"Host preference, life cycle, and classification of species of Ochropsora, including species formerly classified in Aplopsora and Cerotelium pro parte (Pucciniales).","authors":"Yoshitaka Ono","doi":"10.47371/mycosci.2024.10.001","DOIUrl":"10.47371/mycosci.2024.10.001","url":null,"abstract":"Rust species classified in Ochropsora, Aplopsora, and Cerotelium pro parte were re-examined and re-circumscribed by morphology, host preference, life cycle pattern, and geographic distribution. Macrocyclic heteroecious life cycle was proven for seven species by field observations and experimental inoculations. Partial molecular phylogenetic analyses were also included in the taxonomic decision. Anamorphic fungi and others, that were newly discovered and assumed to be related to these genera, were also examined in the same manner. Aplopsora was synonymized under Ochropsora. One fungus named under Cerotelium and two anamorphic fungi were determined as species of Ochropsora. Fifteen species were recognized in Ochropsora: O. ariae, O. asari, O. asiatica, O. corni, O. cumminsii, O. ehimensis, O. dicentrae, O. kraunhiae, O. laporteae, O. lonicerae, O. nambuana, O. nyssae, O. panacis, O. staphyleae, and O. tanakae. Most of Ochropsora species are distributed in eastern Asia. Only O. ariae is known in northwestern Europe and O. cumminsii, O. dicentrae, and O. nyssae are known in eastern North America. The disjunct distribution of Ochropsora in the Northern Hemisphere is interpretated by disjunctions of ancestral species once broadly distributed in the Northern Hemisphere and subsequent species diversification, migration, and extinction in each of the three geographic regions.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"66 1","pages":"1-44"},"PeriodicalIF":1.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A taxonomic re-evaluation of Hymenostilbe mycetophila, a hyperparasitic synnematous hyphomycete on Favolaschia nipponica, with Kobayasiyomyces mycetophilus gen. et comb. nov. (Leotiales). 日本蚕茧上一种超寄生共生菌丝体——嗜菌膜菌（Hymenostilbe mycetophilus gen. et comb）的分类学再评价。11月(Leotiales)。

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-12-06 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.007

Gen Okada, Etsuko Kurokawa, Izumi Sugimoto, Masayuki Nishida, Masahiko Miyai, Yoshiaki Kondo, Akira Hashimoto, Toshiya Iida, Moriya Ohkuma, Yousuke Degawa

{"title":"A taxonomic re-evaluation of Hymenostilbe mycetophila, a hyperparasitic synnematous hyphomycete on Favolaschia nipponica, with Kobayasiyomyces mycetophilus gen. et comb. nov. (Leotiales).","authors":"Gen Okada, Etsuko Kurokawa, Izumi Sugimoto, Masayuki Nishida, Masahiko Miyai, Yoshiaki Kondo, Akira Hashimoto, Toshiya Iida, Moriya Ohkuma, Yousuke Degawa","doi":"10.47371/mycosci.2024.10.007","DOIUrl":"10.47371/mycosci.2024.10.007","url":null,"abstract":"About 70 y after the first report by Yosio Kobayasi, we collected a little-known synnematous hyphomycete, Hymenostilbe mycetophila, on decaying basidiocarps of Favolaschia nipponica growing on dead culms of two bamboo species in Tateyama (on Sasa kurilensis), Chichibu (type locality of H. mycetophila; on Sasamorpha borealis), and Shigakogen (on S. kurilensis) in Japan, and obtained isolates of both the parasite and its host. Kobayasi provided only brief Latin and Japanese descriptions and quite elementary illustrations for H. mycetophila, without depositing any herbarium specimens including the holotype. In this paper, a lectotype and an epitype of this species are designated respectively from the original protolog (illustration) of Kobayasi and our new material collected in Chichibu. Phialidic conidiogenesis and 2- to 3-level verticillate conidiophores terminating in a whorl of 2-5 phialides were newly observed in this fungus on the natural substrate. ITS-LSU sequences of five isolates of H. mycetophila collected in the three locations were identical. Phylogenetic analyses of these markers placed H. mycetophila in Leotiales, although other species of Hymenostilbe are classified in Hypocreales at present. A new genus Kobayasiyomyces for H. mycetophila and a new combination, K. mycetophilus, are proposed here based on our morphological, ecological and phylogenetic data.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"66 1","pages":"72-87"},"PeriodicalIF":1.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-stage wood decay by Grifola frondosa. 灰树花的两阶段木材腐烂。

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-11-30 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.006

Taichi Motoda, Fu-Chia Chen, Daisuke Ando

引用次数: 0

Validity of Diderma microsporum (Myxomycetes) as an independent species supported by morphological and phylogenetic analyses. 形态学和系统发育分析支持小孢子Diderma microsporum（黏菌）作为独立物种的有效性。

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-11-29 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.004

Wen-Long Song, Ya-Jing Chen, Shu-Zhen Yan, Shuang-Lin Chen

{"title":"Validity of Diderma microsporum (Myxomycetes) as an independent species supported by morphological and phylogenetic analyses.","authors":"Wen-Long Song, Ya-Jing Chen, Shu-Zhen Yan, Shuang-Lin Chen","doi":"10.47371/mycosci.2024.10.004","DOIUrl":"10.47371/mycosci.2024.10.004","url":null,"abstract":"Diderma microsporum is a taxonomically controversial species of Myxomycetes. Currently, it is treated as a synonym for Diderma effusum. In this study, two specimens from Lafa Mountain National Forest Park in Jilin Province of China were researched concerning morphology with the light microscope and scanning electronic microscope and compared with the holotype from Japan to confirm that the species was first discovered in China. It is mainly characterized by cushion-shaped plasmodiocarps; single peridium, ornamented with dense flake-like lime crystals on the outer surface; columella absent; capillitium (0.99-)1.29-2.05(-2.30) μm diam, with spindle-shaped enlargement forms at branching points, decorated with dense small warts; spores (5.70-)6.49-6.87(-7.61) μm diam, warted and with clusters of large warts. In addition, the partial nucleotide sequences of nuclear 18S rDNA and elongation factor-1 alpha genes of D. microsporum differed clearly from those of other Diderma species. The results supported the rationality that D. microsporum is an independent species. Light microscopic photos, scanning electron microscopic photos, and an emended description of morphological characters are provided.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"66 1","pages":"58-66"},"PeriodicalIF":1.5,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient generation of uridine/uracil auxotrophic mutants in homokaryotic Cordyceps militaris strains for constructing a food-grade expression platform. 同核蛹虫草菌株尿苷/尿嘧啶营养缺陷突变体的高效生成及食品级表达平台的构建

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-11-29 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.003

Minh Thi Trinh, Khanh-Linh Thi Bui, Hanh-Dung Thai, Tien-Dung Nguyen, Giang Thi Huong Nguyen, Kim-Dung Thi Ha, Hai-Yen Thi Nguyen, Duc-Anh Le, Huong Thi Thu Pham, Sang Van Nguyen, Tao Xuan Vu, Van-Tuan Tran

{"title":"Efficient generation of uridine/uracil auxotrophic mutants in homokaryotic Cordyceps militaris strains for constructing a food-grade expression platform.","authors":"Minh Thi Trinh, Khanh-Linh Thi Bui, Hanh-Dung Thai, Tien-Dung Nguyen, Giang Thi Huong Nguyen, Kim-Dung Thi Ha, Hai-Yen Thi Nguyen, Duc-Anh Le, Huong Thi Thu Pham, Sang Van Nguyen, Tao Xuan Vu, Van-Tuan Tran","doi":"10.47371/mycosci.2024.10.003","DOIUrl":"10.47371/mycosci.2024.10.003","url":null,"abstract":"Cordyceps militaris is a well-known medicinal mushroom widely exploited in traditional medicine and nutraceuticals. In this study, we aim to establish a new platform for improving the production of beneficial ingredients in this fungus. We successfully generated uridine/uracil auxotrophic mutants (ΔpyrG) in five homokaryotic C. militaris strains. The efficiency of the pyrG deletion by homologous recombination reached 100% in all the C. militaris strains. Genetic transformation of the C. militaris ΔpyrG strains mediated by Agrobacterium tumefaciens using the native pyrG auxotrophic marker resulted in high transformation yields of 109-810 transformants per 105 conidia. Additionally, the pyrG marker from Aspergillus oryzae was also functional for the genetic transformation of C. militaris ΔpyrG. We further showed that the gpd1 and tef1 genes were strongly expressed during the mycelial growth of C. militaris, and their promoter sequences were integrated into binary vectors for enhancing recombinant expression. With the constructed platform, the strong heterologous expression of the DsRed protein was proven, and the genomic integration of the endogenous CmFE gene encoding a serine protease under the regulation of the tef1 promoter significantly increased the activity of this enzyme in C. militaris. Our work provides a promising platform for food-grade recombinant expression in C. militaris.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"66 1","pages":"45-57"},"PeriodicalIF":1.5,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new genus and species of Phialocephala-like fungi with cordiform conidia in Chaetosphaeriaceae. 标题毛球科具有堇状分生孢子的类细头真菌一新属新种。

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-11-27 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.11.001

Yu-Hung Yeh, Yao-Moan Huang, Roland Kirschner

引用次数: 0

Coprinopsis cinerea Cla4 protein kinase is required for the assembly of the hyphal tip apparatus that enables the rapid linear growth of apical cells. cl4蛋白激酶是使顶细胞快速线形生长的菌丝尖端装置的组装所必需的。

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.10.005

Tetsuya Kakizaki, Koh Sato, Keishi Osakabe, Hajime Muraguchi

{"title":"Coprinopsis cinerea Cla4 protein kinase is required for the assembly of the hyphal tip apparatus that enables the rapid linear growth of apical cells.","authors":"Tetsuya Kakizaki, Koh Sato, Keishi Osakabe, Hajime Muraguchi","doi":"10.47371/mycosci.2024.10.005","DOIUrl":"10.47371/mycosci.2024.10.005","url":null,"abstract":"The C. cinerea septins are observed at the hyphal tip and septum in the growing vegetative hyphae. In Saccharomyces cerevisiae, septin dynamics have been suggested to be regulated by septin phosphorylation through protein kinases such as Cla4. To gain insight into the relationship between C. cinerea septins and C. cinerea Cla4 protein kinase (Cc.Cla4), we conducted disruption of the C. cinerea cla4 gene (Cc.cla4) by CRISPR/Cas9 system and analyzed the phenotypes of Cc.cla4Δ strains. The vegetative hyphae of the Cc.cla4Δ strains grow slowly in the zigzag shape and are highly branched compared to the wild-type hyphae. The enhanced green fluorescent protein (EGFP) tagged Cc.Cdc3 septin in the Cc.cla4Δ strain was observed in the growing vegetative hyphae. Although EGFP-Cc.Cdc3 signals localized at the plasma membrane of the hyphal tip region, they did not accumulate as a dome with a hole at the hyphal tip as observed in the wild type. Instead, the Cc.Cdc3 was found to stay at the hyphal tip, which split into two tips thereafter. These results suggest that Cc.Cla4 is required for assembly of the hyphal tip apparatus that enables the rapid linear growth of the apical cells.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"307-316"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term effects of forest management on the dynamics of Tricholoma matsutake harvest over 41 years in a Pinus densiflora forest in Nagano Prefecture, Japan. 日本长野县松林41年森林管理对松茸收获动态的长期影响

IF 1.5 4区生物学

Mycoscience Pub Date : 2024-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2024.09.003

Hitoshi Furukawa, Koudai Tokuoka, Aira Mizuno, Kazuhiro Katagiri, Kazuhiko Masuno, Yujiro Tanaka, Shinjiro Oya, Akiyoshi Yamada

{"title":"Long-term effects of forest management on the dynamics of Tricholoma matsutake harvest over 41 years in a Pinus densiflora forest in Nagano Prefecture, Japan.","authors":"Hitoshi Furukawa, Koudai Tokuoka, Aira Mizuno, Kazuhiro Katagiri, Kazuhiko Masuno, Yujiro Tanaka, Shinjiro Oya, Akiyoshi Yamada","doi":"10.47371/mycosci.2024.09.003","DOIUrl":"10.47371/mycosci.2024.09.003","url":null,"abstract":"Tricholoma matsutake is an important wild edible mycorrhizal mushroom in Japan. The domestic matsutake harvest has been declining for 80 y. Nagano Prefecture, a central region of Japan, is the area that has been most productive of matsutake during the past 20 y. To study how matsutake harvesting was affected by forest management, an experimental site was established in a pine forest in Nagano Prefecture in 1980. Two plots were established: a managed plot (MP) in which all trees except pine were cut, pine trees were thinned, and soil litter layer was removed; and a control plot (CP) with no forest management. The numbers of matsutake fruiting bodies were recorded annually in both plots for 41 y. The annual matsutake harvest was stable in the MP but decreased in the CP, while the number of shiro increased in the MP and was stable at the low level in the CP. These trends were suggested to be affected significantly by the thicker humus layer and large root biomass other than pines in the CP. This is the longest record of matsutake harvest in the managed forest, which reveals the importance of such long-term forest management for the sustainable matsutake harvesting.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"65 6","pages":"298-306"},"PeriodicalIF":1.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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