Mycoscience最新文献

Gene expression analysis for stem browning in the mushroom Lentinula edodes 香菇茎干褐变的基因表达分析

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-08-31 DOI: 10.47371/mycosci.2024.07.003

Jili Zhang, Yuki Tanaka, Akiko Ono, Takumi Sato, Toshiyuki Suzuki, Saya Akimoto, Yuki Tanaka, Shoko Iwami, Aya Iwamoto, Norio Tanaka, Naotake Konno, Tomohiro Suzuki

{"title":"Gene expression analysis for stem browning in the mushroom Lentinula edodes","authors":"Jili Zhang, Yuki Tanaka, Akiko Ono, Takumi Sato, Toshiyuki Suzuki, Saya Akimoto, Yuki Tanaka, Shoko Iwami, Aya Iwamoto, Norio Tanaka, Naotake Konno, Tomohiro Suzuki","doi":"10.47371/mycosci.2024.07.003","DOIUrl":"https://doi.org/10.47371/mycosci.2024.07.003","url":null,"abstract":"The mushroom Lentinula edodes, is consumed worldwide and has high industrial value because of its rich content of bioactive compounds such as ergothioneine and eritadenine. Currently, mainstream artificial cultivation methods for this mushroom typically use synthetic logs. However, browning of the stem’s interior (stem browning) has been observed during the cultivation in some L. edodes strains. Although browning does not affect the taste or other qualities of the mushroom, it gives consumers a perception of “poor quality”, and is a major challenge for producers. To identify the genes responsible for stem browning in this mushroom, we performed differential gene expression analysis during stem browning development and quantified it using real-time PCR. Our results indicated that certain oxidoreductases, such as tyrosinase and laccase, were significantly upregulated during the progression of stem browning. The results obtained in the present study provide valuable insights to address the problem of stem browning in mushroom L. edodes.\u0000","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological responses of two Aspergillus oryzae strains to various metal ions at different concentrations 两种黑曲霉菌株对不同浓度的各种金属离子的形态学反应

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-08-23 DOI: 10.47371/mycosci.2024.04.001

Liyun Liu, Kanae Sakai, Takumi Tanaka, Ken-Ichi Kusumoto

{"title":"Morphological responses of two Aspergillus oryzae strains to various metal ions at different concentrations","authors":"Liyun Liu, Kanae Sakai, Takumi Tanaka, Ken-Ichi Kusumoto","doi":"10.47371/mycosci.2024.04.001","DOIUrl":"https://doi.org/10.47371/mycosci.2024.04.001","url":null,"abstract":"Aspergillus species take up various metal ions from environment. The morphology of Aspergillus oryzae strains can vary under the influence of various metal ions. Here, the effects of Ti4+, V3+, Sr2+, Ba2+, Al3+, Fe2+, Zn2+, Mn2+, Ca2+, and Cu2+ on morphological parameters of A. oryzae strains RIB40 and RIB143 were estimated. Colony diameter, conidiation, vesicle head size, and stipe width in both strains varied with concentration. Ti4+, Sr2+, Ba2+, Al3+, Fe2+, and Ca2+ affected conidiation in similar tendency between two strains. The effects of Ti4+, V3+, Sr2+, and Ba2+ on the morphology of A. oryzae are reported here for the first time. Induction of growth of both strains by 0.0001% Ti4+ may help the fermentation industry. Induction of conidiation in RIB40 by 0.001% Cu2+ confirmed previous results that low concentrations of Cu2+ promote the growth of Aspergillus. The most novel finding is that 0.001% Zn2+ increased the vesicle head size in RIB40; possible reasons are discussed.\u0000","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142227727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leucoagaricus karjaticus (Agaricaceae), a new species from Maharashtra, India Leucoagaricus karjaticus（姬松茸科），印度马哈拉施特拉邦的一个新种

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-08-23 DOI: 10.47371/mycosci.2024.07.002

Prashant B. Patil, Nitinkumar P. Patil, Sunita Chahar, Satish Maurya

引用次数: 0

Henon bamboo flowering recorded first time in 120 years revealed how Aciculosporium take affects the floral organs of the host 120 年来首次记录的衡南竹开花揭示了 Aciculosporium 如何影响寄主的花器官

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-08-09 DOI: 10.47371/mycosci.2024.06.001

Eiji Tanaka, Joji Mochizuki

引用次数: 0

Phylogenetic classification and physiological and ecological traits of Metarhizium spp. Metarhizium spp.的系统发育分类和生理生态特征

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-08-01 DOI: 10.47371/mycosci.2024.07.001

Oumi Nishi

{"title":"Phylogenetic classification and physiological and ecological traits of Metarhizium spp.","authors":"Oumi Nishi","doi":"10.47371/mycosci.2024.07.001","DOIUrl":"https://doi.org/10.47371/mycosci.2024.07.001","url":null,"abstract":"The genus Metarhizium (Hypocreales: Clavicipitaceae) is mostly composed of entomopathogenic fungal species. Many of these species are anamorphic and difficult to distinguish morphologically. Furthermore, most isolates of this genus have a broad host range, making classification based on host-insect species uncertain. Molecular phylogenetic analysis based on DNA sequence information distinguishes these species well and revises the taxonomy of Metarhizium. However, in the revisions, the major groups within the genus, such as M. anisopliae complex, were classified regardless of their phenotypic differentiation. Therefore, the characteristics of the individual species remain unclear. To explore the species-specific characteristics of Metarhizium spp., the author performed a phylogenetic analysis and characterization of Metarhizium spp. in Japan. The results showed that strains of the M. brunneum and M. pemphigi clades exhibited cold-active growth characteristics and preferred forested environments over M. pingshaense. In the M. majus clade, a specialist of scarab beetles, isolates from different Scarabaeidae species, including the coconut rhinoceros beetle (Oryctes spp.) and flower chafer beetle (Protaetia orientalis), formed separate subclades and showed strong virulence against their original hosts. This review describes the current state of understanding of the taxonomy and species-specific characteristics of the genus Metarhizium, and includes the author’s own previous study.\u0000","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141885260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life cycle and mating compatibility in the Japanese white jelly mushroom, Tremella yokohamensis 日本白玉菇（Tremella yokohamensis）的生命周期和交配相容性

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-06-29 DOI: 10.47371/mycosci.2024.05.003

Nanthawan Kaeoniwong, Kozue Sotome, Tsuyoshi Ichiyanagi, Norihiro Shimomura, Tadanori Aimi

引用次数: 0

A new species of Fusichalara (Sclerococcaceae, Eurotiomycetes) from Taiwan 来自台湾的 Fusichalara（硬球菌科，欧洲真菌）新种

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-06-29 DOI: 10.47371/mycosci.2024.05.002

Chang-Hsin Kuo, Sung-Yuan Hsieh, Teik-Khiang Goh

引用次数: 0

Lectotypification, epitypification, and molecular phylogenetic confirmation of Cytospora paulowniae comb. nov., a causal pathogen of Paulownia tree canker in Japan 日本泡桐树腐烂病病原菌泡桐胞孢菌（Cytospora paulowniae comb. nov.

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-06-05 DOI: 10.47371/mycosci.2024.02.006

Yukako Hattori, Hayato Masuya, Masato Torii, Toshizumi Miyamoto, Toshiyuki Koiwa, Chiharu Nakashima

引用次数: 0

A useful PCR primer set for the ectomycorrhizal fungus Tricholoma matsutake in wild pine rhizosphere based on the nuclear ribosomal DNA IGS2 sequence 基于核核糖体 DNA IGS2 序列的野生松树根瘤外生菌类松茸的实用 PCR 引物集

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-06-05 DOI: 10.47371/mycosci.2024.05.001

Hiroki Misawa, Yu Tateishi, Yuka Horimai, Aira Mizuno, Fuuma Hida, Hitoshi Furukawa, Hisayasu Kobayashi, Masataka Kawai, Takashi Yamanaka, Masaki Fukuda, Akiyoshi Yamada

{"title":"A useful PCR primer set for the ectomycorrhizal fungus Tricholoma matsutake in wild pine rhizosphere based on the nuclear ribosomal DNA IGS2 sequence","authors":"Hiroki Misawa, Yu Tateishi, Yuka Horimai, Aira Mizuno, Fuuma Hida, Hitoshi Furukawa, Hisayasu Kobayashi, Masataka Kawai, Takashi Yamanaka, Masaki Fukuda, Akiyoshi Yamada","doi":"10.47371/mycosci.2024.05.001","DOIUrl":"https://doi.org/10.47371/mycosci.2024.05.001","url":null,"abstract":"Tricholoma matsutake is an edible ectomycorrhizal mushroom that forms a symbiotic association with Pinaceae trees by constructing a large extraradical mycelial area (called a shiro) in the soil. The detection of this fungal mycelium in the soil is crucial for estimating the success of outplanted mycorrhizal seedlings inoculated with T. matsutake under experimental conditions. Although several T. matsutake-specific DNA markers have been reported for efficient detection in the field, no comparative study has been conducted to assess their effectiveness. In the present study, we targeted the nuclear ribosomal DNA intergenic spacer 2 (IGS2) region for the detection of T. matsutake. The newly designed TmSP-I-2F/TmSP-I-2R primer pair, which targets a partial IGS2 sequence (543 bp), effectively detected T. matsutake from pine root and soil samples via PCR assay, outperforming other T. matsutake-specific primers. In combination with a PCR system targeting LTR DNA markers that were previously developed, a PCR system with the TmSP-I-2F/TmSP-I-2R primer pair set can expedite investigations of the dynamics of T. matsutake genets in mycorrhizas and shiro.\u0000","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening using loop-mediated isothermal amplification (LAMP) assay and breeding of a Saccharomyces cerevisiae strain isolated from Muramatsu Park, Japan, for sake brewing 利用环路介导等温扩增（LAMP）测定筛选和培育从日本村松公园分离出来的酿酒酵母菌株

IF 1.4 4区生物学

Mycoscience Pub Date : 2024-05-31 DOI: 10.47371/mycosci.2024.04.002

Takashi Kuribayashi, Misaki Tsukada, Nanako Asahi, Shin-ichi Kai, Ken-ichi Abe, Mitsuoki Kaneoke, Tetsuya Oguma, Jyunji Kinebuchi, Tomoyuki Shigeno, Tomohito Sugiyama, Daisuke Kasai

引用次数: 0