Mycoscience最新文献

Vezdaea minima, a new species of microlichen from Japan. 日本微地衣一新种。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-09-18 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.06.002

Takato Moriyama, Chihiro Tanaka

{"title":"Vezdaea minima, a new species of microlichen from Japan.","authors":"Takato Moriyama, Chihiro Tanaka","doi":"10.47371/mycosci.2026.06.002","DOIUrl":"10.47371/mycosci.2026.06.002","url":null,"abstract":"The genus Vezdaea consists of lichenized or alga-associated fungi and is characterized by small apothecia without exciple. Unidentified Vezdaea was collected in Japan and morphological observation was performed. The collected samples differed from any known species of the genus Vezdaea in the combination of net-like morphology of paraphyses and the size of the asci and ascospores. Fungal isolates were established from ascospores and goniocysts, and the phylogenetic analysis was performed. The isolates from ascospores and adjacent goniocysts were shown to be conspecific and grouped within the genus Vezdaea. Vezdaea minima is established to accommodate the collected fungus. Species of Vezdaea with and without net-like paraphyses were shown to be not monophyletic respectively. An algal isolate was obtained from the goniocyst, which generally contains a single algal cell, and was nested within Coccomyxa viridis clade as the result of the phylogenetic analysis. Our result indicates that the photobiont of Vezdaea needs further investigation since it has been considered as Leptosira based on a single observation.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 5","pages":"208-212"},"PeriodicalIF":1.4,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metschnikowia okinawaensis sp. nov. and Metschnikowia ryukyuensis sp. nov., two novel yeast species isolated from flowers and young fruits in Okinawa, Japan. 日本冲绳花和幼果中分离的两种酵母新种。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-09-18 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.07.001

Mari Yamamoto, Hiroki Takekata, Natsue Sakata, Hazuki Kotani, Chikara Furusawa, Fumio Matsuda, Taisuke Seike

{"title":"Metschnikowia okinawaensis sp. nov. and Metschnikowia ryukyuensis sp. nov., two novel yeast species isolated from flowers and young fruits in Okinawa, Japan.","authors":"Mari Yamamoto, Hiroki Takekata, Natsue Sakata, Hazuki Kotani, Chikara Furusawa, Fumio Matsuda, Taisuke Seike","doi":"10.47371/mycosci.2026.07.001","DOIUrl":"https://doi.org/10.47371/mycosci.2026.07.001","url":null,"abstract":"Two novel yeast species belonging to the genus Metschnikowia were isolated from flowers and young fruits in Okinawa, Japan. Five strains (JCM 35539, JCM 36740T, JCM 36743, JCM 36744, and JCM 36747) and four strains (JCM 35542, JCM 35544T, JCM 36745, and JCM 38279) were repeatedly isolated from samples collected over 2-3 y. Because BLAST analysis of D1/D2 large subunit ribosomal DNA sequences did not clearly resolve their phylogenetic placement and intragenomic polymorphisms were detected in the internal transcribed spacer region, their relationships were further assessed by whole-genome phylogenetic analysis using available genome data from Metschnikowia and related taxa. This analysis showed that the two novel species formed a distinct clade with Candida danieliae, the closest among described species in the dataset. Analysis of MAT-locus gene collinearity revealed that each novel species possessed strains of opposite mating types, and mating assays between compatible strains produced elongated asci containing needle-shaped ascospores, which is a characteristic feature of the genus Metschnikowia. By integrating phylogenomic, mating, morphological, and physiological evidence, we conclude that these strains represent two novel yeast species, for which the names Metschnikowia okinawaensis sp. nov. and Metschnikowia ryukyuensis sp. nov. are proposed.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 5","pages":"213-222"},"PeriodicalIF":1.4,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13623613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149112286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A macroconidial synanamorph of Kendrickiella phycomyces (Pezizomycotina incertae sedis). 植菌Kendrickiella phycomyces （Pezizomycotina interseis）的一种大分生孢子共形体。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-07-09 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.06.001

Gen Okada, Moriya Ohkuma

引用次数: 0

Effect of disruption of PsPCP gene on fruit body color of Pleurotus salmoneostramineus. PsPCP基因破坏对杏鲍菇果体颜色的影响。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-07-03 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.05.003

Yasuhisa Fukuta, Tatsuro Tatsumi, Shunya Fujita, Norifumi Shirasaka

{"title":"Effect of disruption of PsPCP gene on fruit body color of Pleurotus salmoneostramineus.","authors":"Yasuhisa Fukuta, Tatsuro Tatsumi, Shunya Fujita, Norifumi Shirasaka","doi":"10.47371/mycosci.2026.05.003","DOIUrl":"10.47371/mycosci.2026.05.003","url":null,"abstract":"The pink color of Pleurotus salmoneostramineus is due to a complex of pink color protein (PsPCP) and 2-dehydro-3-deoxylaetiporic acid A, which is a polyene pigment with the yellow color of Laetiporus sulphureus. In this study, we used the Cas9 ribonucleoprotein as a plasmid-free genome-editing tool to perform gene destruction in P. salmoneostramineus. Gene disruption via split-marker recombination by targeting the PsPCP gene using a pre-assembled Cas9/single-guide RNA system was introduced into the protoplasts of the wild-type monokaryotic P. salmoneostramineus strain N13 (A1B1) or N83 (A2B2). Disruptions at the target site were identified using genomic PCR, followed by sequencing. Strains N13 ∆PsPCP and N83 ∆PsPCP were matured on PDA agar plates and then cultivated on sawdust media to form the fruit body of the dikaryotic mutant N13 ∆PsPCP × N83 ∆PsPCP. In addition, compatible mutants of N13 ∆PsPCP × N83 and N13 × N83 ∆PsPCP were cultivated, and the pink coloring of these fruiting bodies was compared. N13 ∆PsPCP × N83 ∆PsPCP showed completely white fruiting bodies. In vitro color-developing reaction using 2-dehydro-3-deoxylaetiporic acid A extracted from the mutant with recombinant PsPCP, which showed no color, suggested that the amount of the compound was trace or degraded during fruit body formation.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 5","pages":"196-202"},"PeriodicalIF":1.4,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13661186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capnofrasera abieticola, a new species of dothideomycetous sooty moulds on Abies veitchii and its molecular phylogenetic position. 黄杉黑霉一新种及其分子系统发育定位。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-06-09 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.05.001

Junta Sugiyama, Akira Hashimoto, Kyung-Ok Nam, Yosuke Degawa, Tsuyoshi Hosoya

{"title":"Capnofrasera abieticola, a new species of dothideomycetous sooty moulds on Abies veitchii and its molecular phylogenetic position.","authors":"Junta Sugiyama, Akira Hashimoto, Kyung-Ok Nam, Yosuke Degawa, Tsuyoshi Hosoya","doi":"10.47371/mycosci.2026.05.001","DOIUrl":"10.47371/mycosci.2026.05.001","url":null,"abstract":"A new species of dothideomycetous sooty mould, Capnofrasera abieticola, is illustrated and described based on fresh specimens collected in Sugadaira, central Japan. It occurred mixed with the subicula of another dominant sooty mould, Metacapnodium neesii (Metacapnodiaceae), on the living twigs and leaves of Abies veitchii (Pinaceae, Pinales). It is morphologically distinguishable from the closest species Capnofrasera flatbergii on branches of Ulmus sp. (Ulmaceae, Rosales) in Sweden by its shorter and narrower phragmoconidia and its host preference. A single phragmoconidium was successfully isolated from the holotype (TNS F-108821) and yield a sterile culture (i.e., no fructifications). Molecular phylogeny based on the concatenated SSU, LSU, and RPB2 dataset revealed that C. abieticola could be assigned to Neoantennariellaceae, not Antennulariellaceae within Capnodiales s. str. (Dothideomycetes).","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 4","pages":"181-189"},"PeriodicalIF":1.4,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13408788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rediscovery and epitypification of Resupinatus merulioides (Resupinataceae, Agaricales) from its type locality in Japan. 在日本的模式地重新发现和分型。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-06-09 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.05.002

Yuki Kurosaki, Kentaro Hosaka

{"title":"Rediscovery and epitypification of Resupinatus merulioides (Resupinataceae, Agaricales) from its type locality in Japan.","authors":"Yuki Kurosaki, Kentaro Hosaka","doi":"10.47371/mycosci.2026.05.002","DOIUrl":"10.47371/mycosci.2026.05.002","url":null,"abstract":"Resupinatus merulioides was originally described from Nikko, Tochigi Prefecture, Japan and characterized by its merulioid hymenophore. Since its description, no additional records have been reported from the type locality, and only a few records have been documented from other regions in Japan. Here, we report some new rediscoveries of R. merulioides from its type locality and other regions in Japan. Newly collected specimens were morphologically consistent with the original description. However, some fruiting bodies had different morphological characteristics such as variations in coloration. Molecular phylogenetic analyses revealed that Japanese specimens identified as R. merulioides formed a well-supported clade, and distinct from other species in this genus. Additionally, specimens labeled as R. merulioides from New Zealand formed a sister clade to R. merulioides s.s. and were genetically distinct. Because of newly observed morphological characters and DNA data, we designate an epitype based on the newly collected specimen from its type locality.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 4","pages":"190-195"},"PeriodicalIF":1.4,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13408782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis of Tulasnella spp. associated with Cypripedium macranthos var. rebunense. 土拉氏菌属与大菱鲆相关的基因组分析。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-06-09 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.04.002

Kota Kambara, Chunyi Jin, Kaien Fujino, Hanako Shimura

{"title":"Genomic analysis of Tulasnella spp. associated with Cypripedium macranthos var. rebunense.","authors":"Kota Kambara, Chunyi Jin, Kaien Fujino, Hanako Shimura","doi":"10.47371/mycosci.2026.04.002","DOIUrl":"10.47371/mycosci.2026.04.002","url":null,"abstract":"Orchid mycorrhizal (OM) fungi are essential for seed germination and seedling development in orchids. Although research on the molecular mechanisms underlying orchids-OM fungi interactions is progressing, detailed analyses, including fungal genomics, are limited. In this study, we performed short-read genome sequencing of two Tulasnella isolates obtained from the terrestrial orchid Cypripedium macranthos var. rebunense-both capable of inducing seed germination. Using sequencing data, we assembled genomes and predicted gene sets, each demonstrating over 95% completeness based on Benchmarking Universal Single-Copy Orthologs analysis, indicating high-quality assemblies and annotations. Phylogenetic analysis incorporating existing Tulasnella genome data revealed that these isolates belonged to Group IV, an early-diverging lineage distant from the other Tulasnella groups. Comparative genomic analyses demonstrated that Group IV Tulasnella possessed a distinct repertoire of genes encoding carbohydrate-active enzymes and lineage-specific small secreted proteins. Our results indicate that these genes are possibly involved in establishing symbiosis with Cypripedium species. These findings offer novel insights into the molecular basis of orchid-mycorrhizal interactions.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 4","pages":"169-180"},"PeriodicalIF":1.4,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13413494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic study of Tulasnella species focusing on their teleomorphs: Descriptions of a new species and two newly recorded species in Japan. 以远形为中心的土拉菌属的分类学研究：日本一新种及两新记录种的描述。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-06-04 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.03.001

Kosuke Nagamune, Nitaro Maekawa, Kenta Fujii, Naoki Endo, Akira Nakagiri, Kenji Suetsugu, Dai Nagamatsu

{"title":"Taxonomic study of Tulasnella species focusing on their teleomorphs: Descriptions of a new species and two newly recorded species in Japan.","authors":"Kosuke Nagamune, Nitaro Maekawa, Kenta Fujii, Naoki Endo, Akira Nakagiri, Kenji Suetsugu, Dai Nagamatsu","doi":"10.47371/mycosci.2026.03.001","DOIUrl":"10.47371/mycosci.2026.03.001","url":null,"abstract":"The genus Tulasnella is generally regarded as saprotrophic, but several species are known to form mycorrhizal associations. Basidioma morphology is a crucial taxonomic character in Tulasnella; however, recent studies have focused on DNA sequences of cultured mycelia isolated from orchid roots for species delimitation and description. In this study, we collected 16 Tulasnella basidioma specimens in western Japan and conducted a taxonomic study based on their morphology, culture properties, and molecular phylogenetic analyses. Phylogenetic analyses of the internal transcribed spacer (ITS) region resolved the specimens into six clades: Tulasnella sp. 1 (T. eichleriana), Tulasnella sp. 4 (a new species described here as T. nematospora), Tulasnella sp. 5 (T. albida), and three unidentified species (Tulasnella spp. 2, 3, and 6). Tulasnella nematospora is described here as a new species based on its thread-like basidiospores, monilioid hyphae forming chains of 2-7 cells on PDA, and multinucleate hyphae. This study provides the first records of T. eichleriana and T. albida from Japan. The close phylogenetic relationship between Tulasnella sp. 6 and an orchid mycorrhizal Tulasnella lineage suggests symbiotic potential in this species, whereas the remaining taxa are presumed to be saprotrophic.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 4","pages":"117-130"},"PeriodicalIF":1.4,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13413496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Independent origins of plant parasitism in Clavicipitaceae: phylogeny, divergence, and taxonomic revision. 树蜂科植物寄生的独立起源：系统发育、分化和分类修正。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-06-04 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.04.001

Eiji Tanaka

{"title":"Independent origins of plant parasitism in Clavicipitaceae: phylogeny, divergence, and taxonomic revision.","authors":"Eiji Tanaka","doi":"10.47371/mycosci.2026.04.001","DOIUrl":"10.47371/mycosci.2026.04.001","url":null,"abstract":"The family Clavicipitaceae (Hypocreales, Ascomycota) includes a wide range of fungal lifestyles and ecological interactions, but the phylogenetic relationships and evolutionary origins of its plant-parasitic taxa have remained controversial. To establish a robust, phylogenetically consistent classification, molecular phylogenetic, fossil-calibrated divergence-time, and likelihood-based ancestral state reconstruction analyses were performed using a multi-locus nuclear dataset. These analyses identified two deeply divergent, monophyletic clades of plant parasites: designated as Clavicipitoideae, comprising Aciculosporium, Balansia, Claviceps, and Epichloë; and Commelinaceomycetoideae, comprising Ustilaginoidea and Commelinaceomyces. Ancestral state reconstruction suggests that plant parasitism evolved independently at least twice within the family. In Clavicipitoideae, plant parasitism most likely originated directly from an invertebrate-associated ancestor, whereas in Commelinaceomycetoideae, it appears to have evolved through a transitional state involving root endophytism coupled with invertebrate parasitism. Divergence-time estimates place the origins of both lineages in the mid-to-late Cretaceous, concurrent with the early diversification of their monocot hosts. On the basis of these evolutionary inferences, major taxonomic revisions are proposed, including the establishment of a new subfamily Commelinaceomycetoideae, a new tribe Commelinaceomyceteae, an emended circumscription of the subfamily Clavicipitoideae, and the validations of the tribes Balansieae, Clavicipiteae, and Ustilaginoideae.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 4","pages":"153-168"},"PeriodicalIF":1.4,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13408790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting a more than century-old study on the mycorrhiza of Gastrodia elata: Rediscovery and scientific significance of slide preparations. 重访天麻菌根一百多年前的研究：载玻片制剂的重新发现和科学意义。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-05-29 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2026.03.003

Takahiro Yagame, Norihisa Matsushita, Chie Hayashi, Hitoshi Neda, Akira Suzuki

引用次数: 0