IF 1.4 4区生物学

Mycoscience Pub Date : 2026-03-20 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2025.12.004

Takashi Kuribayashi, Jumpei Tanaka, Masamichi Sugawara, Keigo Sato, Yoshihito Nabekura, Toshio Joh, Toshio Aoki

{"title":"Fermentation characteristics and industrial potential of kuratsuki sake yeasts isolated from Niigata Prefecture, Japan.","authors":"Takashi Kuribayashi, Jumpei Tanaka, Masamichi Sugawara, Keigo Sato, Yoshihito Nabekura, Toshio Joh, Toshio Aoki","doi":"10.47371/mycosci.2025.12.004","DOIUrl":"https://doi.org/10.47371/mycosci.2025.12.004","url":null,"abstract":"The sake industry continues to evolve in response to consumer demand for novel flavor profiles and fermentation characteristics. However, the genetic and phenotypic diversities of kuratsuki sake yeasts (brewery-specific landraces), which are critical for regional uniqueness and flavor development, remain poorly understood because of limited research and the inherent difficulty of distinguishing these indigenous strains from industrial counterparts. Using molecular biology techniques developed in our laboratory, we successfully isolated indigenous yeast strains from sake breweries in Niigata Prefecture. Subsequent sake fermentation analysis demonstrated substantial phenotypic diversity among these isolates, revealing a wide range of fermentation performance and distinct flavor compound profiles. These findings confirm the continued presence of diverse kuratsuki sake yeasts with industrial potential and highlight their practical applicability in broadening the flavor profiles of sake products.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 2","pages":"49-55"},"PeriodicalIF":1.4,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population density and spore-feeding behavior of collembolans in the presence or absence of myxomycete fruiting bodies. 黏菌子实体存在或不存在情况下的蚁群密度和孢子取食行为。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-02-21 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2025.12.002

Michiko Yano, Taizo Nakamori

{"title":"Population density and spore-feeding behavior of collembolans in the presence or absence of myxomycete fruiting bodies.","authors":"Michiko Yano, Taizo Nakamori","doi":"10.47371/mycosci.2025.12.002","DOIUrl":"https://doi.org/10.47371/mycosci.2025.12.002","url":null,"abstract":"Understanding forest ecosystems involves knowing the role of organisms in dead trees. Myxomycetes that grow on dead trees disperse spores and reuse dead trees as substrate for reproduction. Compared to research on the interactions between myxomycetes and organisms other than Collembola (e.g., Acari, Diptera, and Coleoptera), studies on the interactions between myxomycetes and collembolans on dead trees are limited. To clarify the relationship between them, myxomycetes and nearby collembolans were collected through the aspiration method. This method allows the simultaneous collection of myxomycete fruiting bodies and collembolans. In the laboratory, the collected specimens were observed under a microscope, and the population density and spore-feeding behavior of collembola were analyzed and compared for each myxomycete. In our study, we identified ten families of collembola residing among myxomycetes on dead trees at the collection site. Notably, eight of these families were observed to consume spores and various components of the fruiting bodies of myxomycetes. Additionally, we conducted an analysis of the interrelationships among three species of myxomycetes, focusing on variations in collembolan populations and their feeding rates. This research represents the first quantitative investigation into the relationship between myxomycetes and collembolans, employing field-based observations and collections to yield our findings.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 2","pages":"32-42"},"PeriodicalIF":1.4,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and structural characterization of an anti-Trichophyton compound produced by Auricularia heimuer mycelium. 黑木耳菌丝体抗毛菌化合物的纯化及结构表征。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-02-21 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2025.12.001

Tomoki Hirata, Shengyao Song, Kota Seki, Maki Taniguchi, Atsushi Ishihara, Tsuyoshi Ichiyanagi, Tadanori Aimi

{"title":"Purification and structural characterization of an anti-Trichophyton compound produced by Auricularia heimuer mycelium.","authors":"Tomoki Hirata, Shengyao Song, Kota Seki, Maki Taniguchi, Atsushi Ishihara, Tsuyoshi Ichiyanagi, Tadanori Aimi","doi":"10.47371/mycosci.2025.12.001","DOIUrl":"https://doi.org/10.47371/mycosci.2025.12.001","url":null,"abstract":"In this study, by screening approximately 160 mushroom extracts for anti-Trichophyton activity, significant activity was found in the culture filtrate of the Auricularia heimuer TUFC 100803 strain. To purify the active compound, the strain was cultured in 2.0 L of malt liquid medium, and the culture filtrate was extracted with ethyl acetate. The resulting crude extract was fractionated by silica-gel chromatography and preparative thin-layer chromatography, and a pure active compound, designated heimuerol A, was successfully isolated. According to IUPAC nomenclature, the compound was identified as (1R,2R,5R)-2-(prop-1-en-2-yl)-6-oxabicyclo[3.1.0]hex-3-en-2-ol. Unlike terbinafine, which is used as both a topical and oral medication for tinea, heimuerol A lacks a nitrogen and possesses a structure containing an epoxide group and a five-membered ring. The minimum inhibitory concentration (MIC) of terbinafine-resistant strains was lower than that for wild-type strains when treated with heimuerol A. Furthermore, a mixture of heimuerol A and terbinafine exhibited activity at concentrations lower than their respective MICs. These findings suggest that heimuerol A inhibits ergosterol biosynthesis through a mechanism distinct from that of terbinafine.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 2","pages":"27-31"},"PeriodicalIF":1.4,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Auriscalpium flabellatum sp. nov. (Auriscalpiaceae), a species from Japan producing basidiomata directly from soil. 日本一种直接从土壤中产生担子瘤的木耳。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-02-11 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2025.12.003

Chie Hayashi, Hiroki Sato, Kentaro Hosaka, Hitoshi Neda

引用次数: 0

Origin of the paternal gametes of Japanese whitish truffle (Tuber japonicum) in the second year of truffle production at a konara oak (Quercus serrata) plantation in Kyoto Prefecture, Japan. 在日本京都地区的科那拉橡树（Quercus serrata）种植松露第二年，日本白松露（Tuber japonicum）父系配子的起源。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-02-10 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2025.12.005

Noritaka Nakamura, Akihiko Kinoshita, Yuki Kitade, Muneyoshi Yamaguchi, Takashi Yamanaka

{"title":"Origin of the paternal gametes of Japanese whitish truffle (Tuber japonicum) in the second year of truffle production at a konara oak (Quercus serrata) plantation in Kyoto Prefecture, Japan.","authors":"Noritaka Nakamura, Akihiko Kinoshita, Yuki Kitade, Muneyoshi Yamaguchi, Takashi Yamanaka","doi":"10.47371/mycosci.2025.12.005","DOIUrl":"https://doi.org/10.47371/mycosci.2025.12.005","url":null,"abstract":"Tuber japonicum is an ectomycorrhizal ascomycete fungus native to Japan that produces whitish truffles as one of their reproductive organs. In 2019, a truffle plantation was established in Kyoto Prefecture, Japan, by planting 14 seedlings of konara oak inoculated with two distinct inocula. In November 2022, the first ascocarp production was detected. To contribute to our understanding of the origin of the paternal gametes in this species, we determined the genotypes of 91 second-year ascocarps collected from the site in the autumn of 2023. All of the successfully-analyzed ascocarps possessed one of three maternal multilocus genotypes (MLGs), as identified by simple sequence repeat (SSR) marker analysis, two of which were identical to the paternal and maternal genotypes identified in ascocarps harvested the previous year. The number of paternal MLGs identified increased markedly from 2 in 2022 to 53 in 2023, 48 of which were found in only a single ascocarp. All paternal MLGs were either identical to a maternal one or a random recombination between them, indicating that the paternal gametes were derived from ascospores formed the previous year. Thus, this study clearly demonstrates that the majority of paternal mating partners in T. japonicum originate from the previous year's ascospores.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 2","pages":"56-64"},"PeriodicalIF":1.4,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-examination of the morphological and molecular features of Amanita clarisquamosa and Amanita avellaneosquamosa based on specimens collected in Hokkaido. 以北海道地区为研究对象，重新研究了clarisquamosa和avellaneosquamosa的形态和分子特征。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-01-20 eCollection Date: 2026-01-01 DOI: 10.47371/mycosic.2025.11.001

Minoru Nakai, Akihiro Yamaguchi, Hironobu Suga, Hiroko Shirayama, Takahito Kobayashi, Takahiro Suzuki, Jiro Koizumi, Tsutomu Abe, Yasuhiro Funatsu, Yoriko Sugiyama

{"title":"Re-examination of the morphological and molecular features of Amanita clarisquamosa and Amanita avellaneosquamosa based on specimens collected in Hokkaido.","authors":"Minoru Nakai, Akihiro Yamaguchi, Hironobu Suga, Hiroko Shirayama, Takahito Kobayashi, Takahiro Suzuki, Jiro Koizumi, Tsutomu Abe, Yasuhiro Funatsu, Yoriko Sugiyama","doi":"10.47371/mycosic.2025.11.001","DOIUrl":"https://doi.org/10.47371/mycosic.2025.11.001","url":null,"abstract":"Amanita clarisquamosa and A. avellaneosquamosa, both of which belong to the section Amidella, were described in 1933 in Nopporo, Hokkaido, Japan. Owing to large intraspecific variation and high interspecific resemblance in morphology, species delimitation and identification of section Amidella members is difficult. This also applies to the two focal species. Furthermore, the minimality of the original descriptions of A. clarisquamosa and A. avellaneosquamosa exacerbates this problem. For reliable identification, detailed morphological and molecular information of reliably identified specimens, hopefully type specimens is required. However, further morphological and molecular information cannot be obtained from the degraded type specimens. In this study, new specimens that showed morphological and molecular matches with A. clarisquamosa and A. avellaneosquamosa were obtained from Nopporo and the surrounding areas of Hokkaido. Detailed morphology and barcoding region sequences of these specimens were recorded. Importantly, the newly collected specimens showed morphological and molecular mismatches with previously reported A. clarisquamosa or A. avellaneosquamosa specimens. This indicated that specimens previously identified as these two species might have included misidentifications. Overall, the study results suggest a need for reconfirmation of species within the section Amidella.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 1","pages":"10-19"},"PeriodicalIF":1.4,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13062949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147675183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of fungal contamination on museum books stored under controlled environmental conditions: A discrepancy between culture-based and metagenomic analysis approaches. 受控环境条件下博物馆图书真菌污染的检测：基于培养和宏基因组分析方法的差异。

IF 1.4 4区生物学

Mycoscience Pub Date : 2026-01-20 eCollection Date: 2026-01-01 DOI: 10.47371/mycosic.2025.11.002

Isoto Yoshioka, Chie Hayashi, Yasunobu Endo, Aoi Sawada, Yugo Mori, Sayaka Ban, Takashi Yaguchi

{"title":"Detection of fungal contamination on museum books stored under controlled environmental conditions: A discrepancy between culture-based and metagenomic analysis approaches.","authors":"Isoto Yoshioka, Chie Hayashi, Yasunobu Endo, Aoi Sawada, Yugo Mori, Sayaka Ban, Takashi Yaguchi","doi":"10.47371/mycosic.2025.11.002","DOIUrl":"https://doi.org/10.47371/mycosic.2025.11.002","url":null,"abstract":"Mold contamination in library and museum collections poses risks to both cultural heritage and human health. This study examined fungal flora on books stored under controlled environmental conditions (temperature <20 °C, relative humidity <50%) in The University Museum, The University of Tokyo. Both culture-dependent methods and DNA-based metabarcoding targeting the internal transcribed spacer 2 region were used. DNA analysis revealed that Aspergillus halophilicus accounted for over 90% of the sequences from six books. In contrast, culture-based methods using standard media (e.g., PDA, DG18, M40Y) primarily isolated species such as Aspergillus, Penicillium, and Cladosporium, but not A. halophilicus. However, cultivation on CzA supplemented with 70% sucrose at lower temperatures enabled successful isolation of A. halophilicus from one sample. The strain was identified based on morphological features and β-tubulin gene analysis. These findings demonstrate a notable discrepancy between molecular and culture-based results, underscoring the limitations of conventional media for detecting xerophilic fungi in dry environments. The study suggests that desiccation-tolerant species like A. halophilicus can thrive even under strict storage controls and may evade standard integrated pest management (IPM) protocols. To better assess fungal risks in preservation settings, combining improved media with DNA-based methods is essential.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"67 1","pages":"20-26"},"PeriodicalIF":1.4,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13062915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147675255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbotryum crypticum, a new anther smut fungus on Silene miqueliana from Japan. 日本密莲花药黑穗病真菌的一种新发现。

IF 1.4 4区生物学

Mycoscience Pub Date : 2025-12-20 eCollection Date: 2026-01-01 DOI: 10.47371/mycosci.2025.10.001

Naoko Fujita, Akira Hashimoto, Moriya Ohkuma, Tsuyoshi Hosoya, Makoto Kakishima

引用次数: 0

The multi-locus phylogeny reveals a cryptic species within the Suillus grevillei complex in eastern Eurasia. 多位点系统发育揭示了欧亚大陆东部的Suillus grevillei复合体中的一个隐种。

IF 1.4 4区生物学

Mycoscience Pub Date : 2025-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2025.09.002

Yumiko Miyamoto, Yutaka Tamai, Trofim C Maximov, Atsuko Sugimoto, Akiyoshi Yamada

{"title":"The multi-locus phylogeny reveals a cryptic species within the Suillus grevillei complex in eastern Eurasia.","authors":"Yumiko Miyamoto, Yutaka Tamai, Trofim C Maximov, Atsuko Sugimoto, Akiyoshi Yamada","doi":"10.47371/mycosci.2025.09.002","DOIUrl":"https://doi.org/10.47371/mycosci.2025.09.002","url":null,"abstract":"Suillus grevillei is an ectomycorrhizal fungus of larch that occurs widely throughout the Northern Hemisphere. According to its pileus color and geographic distributions, two closely related species are recognized: European S. grevillei that has a yellowish pileus and North American S. clintonianus that has a reddish pileus. Because specimens in eastern Eurasia are understudied, we incorporated basidioma collections from Japan and eastern Siberia to clarify the taxonomy and distribution of the S. grevillei complex in a global context. The multi-locus phylogeny assembled with four conservative loci (LSU, RPB1, RPB2, and TEF) revealed three monophyletic clades with strong branching support, one of which is described as a new species, S. orientalis. Morphologically, S. orientalis is distinguished from S. grevillei by its dark reddish-brown pileus and from S. clintonianus by having smaller spores and the absence of encrusted hyphae in the pileipellis. S. orientalis is distributed in eastern Eurasia, S. grevillei in Europe and eastern Asia, and S. clintonianus in North America. The multi-locus phylogeny of conservative DNA markers was more effective than rapidly evolving and highly variable ITS regions in recognizing closely related species within the S. grevillei complex.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"66 6","pages":"350-360"},"PeriodicalIF":1.4,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13062965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147675106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three new tricholomatoid dark blue Entoloma spp. from temperate forests in Japan, with the redescription of E. cyanonigrum. 日本温带森林中三新种类口蘑深蓝肠虫属，并附蓝肠虫的重新描述。

IF 1.4 4区生物学

Mycoscience Pub Date : 2025-11-20 eCollection Date: 2025-01-01 DOI: 10.47371/mycosci.2025.07.007

Yasukazu Ohkubo, Daisuke Sakuma, Hiroshi Masumoto

{"title":"Three new tricholomatoid dark blue Entoloma spp. from temperate forests in Japan, with the redescription of E. cyanonigrum.","authors":"Yasukazu Ohkubo, Daisuke Sakuma, Hiroshi Masumoto","doi":"10.47371/mycosci.2025.07.007","DOIUrl":"https://doi.org/10.47371/mycosci.2025.07.007","url":null,"abstract":"Three species of tricholomatoid dark blue Entoloma spp. from temperate forests in Japan are described as new to science. Entoloma obscurocyaneum and E. purpureobrunneolum show a dull dark blue pileus, with the former distinguished by the purple-brown suprapellis hyphae of the pileipellis and the latter by its light brown color. Entoloma quasicyanonigrum is similar to E. cyanonigrum but exhibits smaller basidiospores. Our phylogenetic analysis placed them within a moderately supported clade that generally shares the characteristics of having a hymeniderm- or palisadoderm-type pileipellis. To date, the sole tricholomatoid dark blue Entoloma documented from Japan is E. cyanonigrum. Because it was confirmed that the type specimen of E. cyanonigrum no longer exists, the original material, the colored drawing by Tsuguo Hongo, was designated as the lectotype, and E. cyanonigrum was redescribed based on Hongo's observation notes and our examination of herbarium specimens.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"66 6","pages":"322-333"},"PeriodicalIF":1.4,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13062928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147675073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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