Mycologia最新文献

{"title":"Genomic analyses and mating tests among sympatric isolates of <i>Beauveria bassiana</i> provide evidence for a diverse cryptic species complex.","authors":"Leela M Rizal, James P Hereward, Alistair R McTaggart, Michael J Furlong, Gimme H Walter","doi":"10.1080/00275514.2025.2568223","DOIUrl":"10.1080/00275514.2025.2568223","url":null,"abstract":"<p><p>We investigated the extent of recombination among isolates of <i>Beauveria bassiana</i> s.l. to assess whether gene flow occurs among them in nature and infer whether non-outcrossing populations may represent cryptic species. We sequenced and assembled genomes of 14 isolates collected from soil in Queensland, Australia, and each was genotyped at their mating type locus. We reconstructed a phylogeny from sequences of <i>B. bassiana</i> available on the National Center for Biotechnology Information Sequence Read Archive (NCBI SRA) database and those generated in this study. The isolates from Queensland sequenced in this study form a distinct clade relative to the isolates from other countries on NCBI, and they separated into four genetic groups with high pairwise <i>F</i>_ST values between them. One of these groups had both <i>MAT1-1</i> and <i>MAT1-2</i> mating types, but the other three each had only a single mating type. We crossed isolates from the different genetic groups on Sabouraud dextrose agar yeast extract (SDAY) solid medium and used vegetative compatibility and the production of sexual structures as measures of whether sexual or parasexual recombination is likely to occur in nature. Vegetative compatibility was found within genetic groups when opposite mating types were crossed, with the production of synnemata and perithecia-like structures. However, sexual structures (perithecia, asci, or ascospores) did not develop. Isolates from the different genetic groups were not vegetatively compatible when crossed. We used analyses of linkage disequilibrium to test for evidence of past recombination among the Queensland genetic groups. Two of them had low indices of association and were reticulate in network analyses, which supports recombination (and therefore sexual or parasexual reproduction) within these two genetic groups, but we found no evidence for recombination among the four genetic groups. Our results indicate that <i>B. bassiana</i> is a diverse complex of multiple cryptic species.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"21-39"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145489344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species diversity of <i>Diaporthe</i> on mangroves: Taxonomic novelties, pathogenic associations, and phylogenetic reassessment of endophytes.","authors":"Herbert Dustin R Aumentado, K W Thilini Chethana, Ruvishika S Jayawardena, Chayanard Phukhamsakda, Chada Norphanphoun, E B Gareth Jones, Ali H Bahkali, Kevin D Hyde","doi":"10.1080/00275514.2025.2550907","DOIUrl":"10.1080/00275514.2025.2550907","url":null,"abstract":"<p><p><i>Diaporthe</i> species are frequently reported as endophytes on mangroves. These species are also known pathogens affecting several important hosts worldwide, but have yet to be explored as pathogens in mangrove environments. In this study, symptomatic leaves and stems of several mangrove species were collected from mangrove forests and estuaries in Thailand. Forty-five <i>Diaporthe</i> strains were isolated from symptomatic leaves and stems of various mangrove species, associated with leaf spots and stem cankers, respectively. A polyphasic identification approach, comprising morphological, multilocus phylogenetic analyses (ITS, <i>TEF1-α</i>, <i>TUB2, CAL</i>, and <i>HIS3</i>), and pairwise homoplasy index tests, identified two new species complexes, 10 <i>Diaporthe</i> species belonging to three sections (Eres, Foeniculina, and Sojae). This included <i>D. mayteni</i> species complex, <i>D. tanakae</i> species complex, three novel species: <i>Diaporthe mangroviorum, D. narathiwatensis</i>, and <i>D. rhizophoracearum</i>, as well as 19 new host-pathogen associations for <i>D. arecae, D. azadirachtae, D. biconispora, D. charlesworthii, D. siamensis, D. ueckeri</i>, and <i>D. vitimegaspora</i>, and two new geographic records for <i>D. azadirachtae</i> and <i>D. charlesworthii</i>. Pathogenicity was confirmed on their original mangrove hosts using detached leaf assays, showing varying levels of pathogenicity. We also present evidence supporting synonymizing of <i>D. eleutharrhenae, D. pseudobauhiniae, D. xishuangbannaensis</i>, and <i>D. yunnanensis</i> with <i>D. vitimegaspora</i>. Furthermore, the taxonomic placement and identity of previously reported mangrove endophytic <i>Diaporthe</i> species have been updated. Based on this and previous studies, a total of 93 strains belonging to 16 <i>Diaporthe</i> species are associated with mangrove species. The findings of this study gave us a more holistic understanding of the <i>Diaporthe</i> species associated with mangroves, their lifestyles, etiology, and host range.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"47-101"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new species of <i>Coltricia</i> (Hymenochaetales, Basidiomycota) from West Africa based on morphological and molecular phylogenetic evidence.","authors":"Boris Armel Olou, Meike Piepenbring, Nourou Soulemane Yorou","doi":"10.1080/00275514.2025.2559555","DOIUrl":"10.1080/00275514.2025.2559555","url":null,"abstract":"<p><p><i>Coltricia</i> (Hymenochaetales, Hymenochaetaceae) is a cosmopolitan group of wood-inhabiting fungi known for its ecological role in nutrient cycling and ectomycorrhizal associations, particularly in tropical ecosystems. Species diversity of <i>Coltricia</i> in tropical West Africa is poorly explored, with only four species previously documented from the region. Recent field surveys were conducted in Benin, Guinea, and Togo, leading to the collection of six <i>Coltricia</i> specimens. Through a combination of morphological and molecular analyses, two new species, <i>Coltricia langeri</i> and <i>Coltricia mosseboi</i>, were identified and described. Phylogenetic analyses based on sequence data of the internal transcribed spacer (ITS), large subunit ribosomal DNA (28S), and translation elongation factor 1-alpha (<i>EF1α</i>) regions confirmed the placement of these new species within <i>Coltricia</i>. Our results also revealed the polyphyletic nature of <i>Coltricia</i> and its close relationship with <i>Coltriciella</i>, challenging current generic boundaries. A dichotomous key to the six <i>Coltricia</i> species now known from tropical Africa is provided. This study expands the known diversity of <i>Coltricia</i> in Africa to six, contributing to the understanding of fungal diversity in tropical forests. The discovery of these new species highlights the need for further exploration and conservation of fungal species in West African ecosystems.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"130-142"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145409440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and taxonomy of the genera of Erysiphaceae, part 8: <i>Podosphaera</i> sect. <i>Tridactyla</i>.","authors":"Michael Bradshaw, Uwe Braun, Uma Crouch, Andrew Paul, James K Mitchell, Jacklyn Thomas, Donald H Pfister","doi":"10.1080/00275514.2025.2554558","DOIUrl":"10.1080/00275514.2025.2554558","url":null,"abstract":"<p><p>The eighth part of a series devoted to the phylogeny and taxonomy of powdery mildews is presented. In this part, the general phylogeny and taxonomy of the genus <i>Podosphaera</i> are treated, including a new division of this genus into sections: <i>Podosphaera</i> sect. <i>Podosphaera, Podosphaera</i> sect. <i>Magnicellulatae</i>, comb. nov. and the reintroduced <i>Podosphaera</i> sect. <i>Tridactyla</i>. Sect. <i>Podosphaera</i> consists of two morphologically deviating subsections (<i>Podosphaera</i> and <i>Sphaerotheca</i>). Subsect. <i>Podosphaera</i> consists of species that have apically dichotomously branched chasmothecial appendages, whereas subsect. <i>Sphaerotheca</i> consists of species with unbranched, mycelioid chasmothecial appendages. These subsections are not monophyletic and thus are based solely on morphology. The phylogeny and taxonomy of species of sect. <i>Tridactyla</i> are treated based on ITS+28S data. New primers for the protein-coding genes glutamine synthetase (<i>GS</i>), RNA polymerase II subunit B (<i>RPB2</i>), and β-tubulin (<i>TUB2</i>) are generated for the genus <i>Podosphaera</i> and provided as a resource for future research. A representative multilocus tree for the entire genus, including all sections, is presented. Additionally, the new combination <i>Podosphaera brayana</i> is introduced. This name has priority over <i>Po. ampla. Erysiphe brayana</i> is lecto- and epitypified, and a lectotype is designated for <i>Sphaerotheca phytoptophila</i>. The present analyses aim to provide resources for future research evaluating powdery mildews of the genus <i>Podosphaera</i> and, in particular, <i>Podosphaera</i> sect. <i>Tridactyla</i>.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"116-129"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cantharellus</i> diversity in mixed oak forests of Virginia, with a new species, <i>Cantharellus sabuletorum</i>.","authors":"Devin Bily, Rachel A Swenie, Tashi Gyatso, Matthew E Smith, Brandon Matheny","doi":"10.1080/00275514.2025.2559556","DOIUrl":"10.1080/00275514.2025.2559556","url":null,"abstract":"<p><p>Chanterelles (genus <i>Cantharellus</i>) are ectomycorrhizal fungi associated with a broad host range of trees. We explored <i>Cantharellus</i> diversity in mixed oak forests across 12 counties in Virginia and constructed a multilocus phylogeny including large ribosomal subunit (28S), transcription elongation factor 1-alpha (<i>tef1</i>), and RNA polymerase II (<i>rpb2</i>) sequences from all known temperate species of <i>Cantharellus</i>. Habitat and morphological attributes, including spore size, shape, and color, were compared with described species. In total, 104 sequences from 31 specimen vouchers were generated and 11 taxa identified, including one new species, <i>C. sabuletorum</i> from sandy <i>Quercus-Pinus</i> forests of Chesapeake Bay and north-central Florida. This study also provides evidence of genetic and color variation within <i>C. cinnabarinus</i> and expands the known distribution of <i>C. altipes, C. appalachiensis, C. flavolateritius, C. lateritius, C. lewisii, C. minor, C. tenuithrix</i> complex, <i>C. vicinus</i>, and <i>C. velutinus</i> in the eastern United States.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"143-162"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First evidence of clavicipitaceous fungus attacking amphibians.","authors":"Tsuyoshi Hosoya, Tomotaka Sato, Sho Kadekaru, Yumi Une","doi":"10.1080/00275514.2025.2560298","DOIUrl":"10.1080/00275514.2025.2560298","url":null,"abstract":"<p><p><i>Metapochonia bulbillosa</i> (Clavicipitaceae) was identified as the causative agent of a novel mycosis affecting <i>Pelophylax porosus brevipodus</i>, an endangered frog species in Japan. Analyses of spontaneous disease outbreaks and experimental infections confirmed that the fungus can cause lethal infections in both <i>Pelophylax porosus brevipodus</i> and <i>Dryophytes japonicus</i>. This study provides the first evidence that a member of the Clavicipitaceae, a family previously known primarily for plant and insect pathogens, can also cause disease in amphibians.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"40-46"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145409456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling anamorph-teleomorph connections allows proper classification of chalara‑like anamorphs of Helotiales and Chaetosphaeriales.","authors":"Ondřej Koukol, Petra Seifertová","doi":"10.1080/00275514.2025.2562791","DOIUrl":"10.1080/00275514.2025.2562791","url":null,"abstract":"<p><p>The taxonomy of chalara-like anamorphs in Leotiomycetes was recently thoroughly redefined. Due to the morphological simplicity of these species, emphasis was given on monophyletic generic concepts defined based on rDNA. In our follow-up study, we revised the taxonomic classification of selected chalara-like fungi in the families Hamatocanthoscyphaceae and Pezizellaceae. We examined our isolates of chalara-like anamorphs and collections of discomycete teleomorphs from coniferous litter in Europe, along with a revision of related fungarium collections. We performed phylogenetic analyses based on data sets consisting of the internal transcribed spacer region ITS1-5.8S-ITS2, partial 28S nuc rDNA, and fragments of genes encoding either translation elongation factor 1α or RNA polymerase II second largest subunit. Our phylogenetic analyses showed that the inclusion of teleomorphs is essential for proper naming of the holomorphic species. In Hamatocanthoscyphaceae, we propose to synonymize the monotypic genus <i>Chalarodendron</i> with the recently erected genus <i>Stipitochalara</i>. Further, we provide evidence of <i>Infundichalara microchona</i> being the anamorph of <i>Ciliolarina ligniseda</i>. The anamorph for <i>Hamatocanthoscypha laricionis</i>, the type species of <i>Hamatocanthoscypha</i>, is also documented. Several sequences obtained from collections identified as <i>H. laricionis</i> were distantly placed in the phylogeny, indicating that a thorough revision of the genus is needed and its relationship with <i>Constrictochalara</i> and <i>Xenochalara</i> should be explored. In Pezizellaceae, we reveal the anamorph-teleomorph connection between <i>Calycina</i> (=<i>Chalara</i>) <i>fungorum</i> and <i>Calycina subtilis</i> and propose to synonymize <i>Nagrajchalara angustata</i> with <i>N. inflatipes. Calycina</i> (=<i>Chalara</i>) <i>brevispora</i> should be excluded from Leotiomycetes; molecular data from a recently obtained isolate showed that this fungus should be placed in <i>Chalarina</i> (Chaetosphaericaceae, Chaetosphaeriales, Sordariomycetes).</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"163-189"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145564687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new homothallic species of <i>Alternaria</i> section <i>Nimbya</i> from wetland plants in Iran.","authors":"Zahra Alavi, Abdollah Ahmadpour, Youbert Ghosta","doi":"10.1080/00275514.2025.2572908","DOIUrl":"10.1080/00275514.2025.2572908","url":null,"abstract":"<p><p>During a survey of fungi associated with Cyperaceae plants in northern and northwestern Iran, three previously undescribed species belonging to <i>Alternaria</i> section <i>Nimbya</i> were identified and characterized. These new species, namely, <i>Alternaria azarbaijanica, A. caspica</i>, and <i>A. eleocharidis</i>, spp. nov. were examined using a combination of morphological traits, cultural features, and molecular data. Remarkably, all three species produced both sexual and asexual morphs under laboratory conditions, providing rare insights into reproductive modes within <i>Alternaria</i>, where sexual morphs are rarely observed. Phylogenetic analyses based on five gene regions (ITS-rDNA, <i>GAPDH, TEF1, RPB2</i>, and <i>Alt a 1</i>) clarified their evolutionary relationships and supported species delimitation. Phylogenetic trees constructed using maximum likelihood, maximum parsimony, and Bayesian inference consistently resolved each species as a distinct monophyletic lineage within <i>Alternaria</i> section <i>Nimbya</i>. These findings reveal previously unrecognized <i>Alternaria</i> diversity on Cyperaceae hosts and underscore the value of combining morphological observations with multilocus phylogenetic approaches to resolve taxonomic complexities in <i>Alternaria</i>.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"214-232"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145564624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Septoria araujiae</i>: Phylogeny, pathogenicity, and host specificity of a fungal pathogen with potential as a biocontrol agent for moth plant.","authors":"Gustavo Hernán Ramírez, María Virginia Bianchinotti, Freda Elizabeth Anderson","doi":"10.1080/00275514.2025.2564582","DOIUrl":"10.1080/00275514.2025.2564582","url":null,"abstract":"<p><p>Native to South America, moth plant (<i>Araujia hortorum</i>, Apocynaceae) is an invasive vine that threatens natural ecosystems and agriculture in many parts of the world. Biological control studies involving fungal pathogens have primarily focused on the rust <i>Puccinia araujiae</i>, but other aggressive pathogens exist that may show potential as complementary agents. In its native range in Argentina, plants were quite frequently observed with severe leaf and fruit damage caused by a fungal disease. The pathogen associated with this disease was isolated and identified as <i>Septoria araujiae</i> based on morphological analyses. Multilocus analyses allowed the first phylogenetic placement of this species within the genus. Koch's postulates were fulfilled through inoculation experiments, confirming <i>S. araujiae</i> as the causal agent. The pathogen exhibited a hemibiotrophic life cycle, with an extended asymptomatic phase followed by a necrotrophic stage that led to severe defoliation. Host specificity experiments revealed a narrow host range, with susceptibility largely confined to species within the subtribe Oxypetalinae. These findings contribute new insights into the diversity, ecology, and host interactions of <i>Septoria</i> species and highlight the potential of <i>S. araujiae</i> as a biological control agent for <i>A. hortorum</i>.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"102-115"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Hericium americanum</i> var. <i>roseum</i>-a new North American taxon.","authors":"Timothy J Baroni, Todd W Osmundson","doi":"10.1080/00275514.2025.2588831","DOIUrl":"10.1080/00275514.2025.2588831","url":null,"abstract":"<p><p>A rarely collected rose-pink-colored <i>Hericium americanum</i> has now been documented with in situ images obtained by 11 different field naturalists in the northeastern United States and eastern Canada since 2018. These mushroom enthusiasts responded to a request for images and specimens of a rarely documented rose-pink-colored tooth fungus discussed in the comments section of a description of <i>Hericium americanum</i> and <i>H. coralloides</i> in <i>Mushrooms of the Northeastern United States and Eastern Canada</i>. Five of those finds were collected, preserved by drying, and sent to the authors for analysis. This strikingly colored tooth fungus is confirmed as a variant of <i>H. americanum</i> using morphological and molecular data (nuc rDNA ITS1-5.8S-ITS2 and partial nuc 28S rDNA D1-D6 regions) and proposed here as a new variety.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"233-241"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}