Mycologia最新文献

{"title":"Single-cell transcriptomics reveals a new marine parasite: Characterization and distribution of <i>Rozellomyces</i> from the Gulf of Alaska.","authors":"Vedprakash G Hurdeal, Elizabeth C Cooney, Gordon Lax, Anthony M Bonacolta, Patrick J Keeling, Naomi M Fast","doi":"10.1080/00275514.2026.2631200","DOIUrl":"10.1080/00275514.2026.2631200","url":null,"abstract":"<p><p>Parasitic microorganisms play critical roles in shaping aquatic ecosystems, yet many lineages, such as those within the phylum Rozellomycota, remain poorly understood. Here, we describe a new rozellid isolate from the Gulf of Alaska. Phylogenomic analysis based on 238 genes confirms its placement within Rozellomycota and its distinctness from currently described taxa. We identified amplicon sequence variants (ASVs) from the metaPR² database to recover its geographic distribution. Our isolate is an endoparasite of the diatom <i>Thalassiosira</i>, a primary producer in marine ecosystems that plays a critical role in global carbon and nutrient cycling. Phylogenetic and ecological evidence supports the establishment of a new genus within Rozellomycota, <i>Rozellomyces</i>. Our findings provide new insights into the diversity, distribution, and ecological roles of Rozellomycota, highlighting the need for further research on these parasites in marine ecosystems.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"708-717"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147504069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dikaryon formation in <i>Ustilago syntherismae</i>: A key life-history trait in its interaction with <i>Digitaria sanguinalis</i>.","authors":"Antoni M C Verdú, M Teresa Mas","doi":"10.1080/00275514.2026.2619990","DOIUrl":"10.1080/00275514.2026.2619990","url":null,"abstract":"<p><p>Loose smut sterilizing annual large crabgrass has occurred since 2004 in a field surrounded by crops near Barcelona (Spain); no other patch of smut-diseased plants has been seen in the nearby area. In order to understand the coexistence of both partners over the years, (i) the formation of the dikaryotic hyphae were investigated under fluorescence microscopy and (ii) spore viability was assessed by quantifying the proportion of germinated ustilospores from sori collected during three consecutive fall seasons in the same field. The dikaryon is formed by intratetrad mating; in none of the media tested could the formation of sporidia be observed. On average, 7.1% of ustilospores were able to germinate in a liquid medium without any organic carbon source following 2 years of storage. The role that these findings could play in disease escape and thus in the plant-pathogen coexistence at local field scale is discussed in light of the results.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"743-748"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species delimitation in a cryptic Neotropical <i>Thelephora</i> species complex, with insights into the phylogenetic informativeness of loci.","authors":"Ezequiel Alberto Cruz-Campuzano, Mariana Avila Muñoz, Carla Elisa Silva-Tostado, Alejandro Estrada Avendaño, Rodolfo Salas-Lizana, Eduardo Hernández-Navarro, Peter Kennedy, Matthew E Smith, Kurt O Miller, Alan R Franck, Julieta Alvarez-Manjarrez","doi":"10.1080/00275514.2026.2651040","DOIUrl":"10.1080/00275514.2026.2651040","url":null,"abstract":"<p><p><i>Thelephora</i> is a genus of ectomycorrhizal fungi with a global distribution. Recent taxonomic efforts have revealed new species and species complexes, often distinguished by subtle morphological differences. Here, we describe <i>Thelephora renispora</i>, a new species resembling <i>T. versatilis</i> and <i>T. pseudoversatilis</i>, and support its recognition as a distinct taxon based on morphological traits, phylogenetic analyses of nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS barcode) and D1-D2 domains of nuc 28S rDNA (28S), and statistical comparisons of its micromophology against closely related allies. New distribution records for <i>T. pseudoversatilis</i> are also provided. Additionally, we present phylogenetic informativeness analyses to assess the utility of ITS, 28S, and other markers previously used for the order Thelephorales. Our results identify the DNA-directed RNA polymerase II second-largest subunit (<i>RPB2</i>) as the most informative locus for delimiting species-level relationships in the Thelephorales order, whereas ITS contributes to solving interspecific relations within the <i>Thelephora-Tomentella</i> clade.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"828-848"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monomethylhydrazine content is determined by altitude in <i>Gyromitra antarctica</i>: Implications for safe consumption.","authors":"Romina B Parada, Roberto Garibay Orijel, Andrés de Errasti, María Belén Pildain, Carolina Barroetaveña","doi":"10.1080/00275514.2026.2641837","DOIUrl":"10.1080/00275514.2026.2641837","url":null,"abstract":"<p><p>Several species of the genus <i>Gyromitra</i>-commonly known as \"false morels\"-are traditionally consumed in different parts of the world, despite their potential toxicity due to the presence of monomethylhydrazine, a volatile compound. In some taxa, monomethylhydrazine content has been linked to environmental factors such as elevation, potentially influencing toxicity levels. In southern South America, ethnomycological records indicate that <i>Gyromitra</i> species are collected and consumed by indigenous communities. However, their taxonomy and toxicological profile remain poorly understood. In this study, we examined the phylogenetic identity and hydrazine content of <i>Gyromitra</i> specimens collected across a Patagonian elevation gradient. Molecular analyses based on nuclear internal transcribed spacer, large subunit, and elongation factor-1 alpha sequences confirmed the identity of all specimens as <i>G. antarctica</i>. Hydrazine content was quantified in 82 fresh samples collected between 300 and 800 m above sea level, revealing a significant negative correlation between elevation and toxin concentration (ρ = -0.79, <i>P</i> < 0.001). Two boiling treatments -10 min without water exchange, and 2 × 5 min with water exchange-reduced hydrazine levels by 97% and 94%, respectively. However, residual toxins were detected in the boiling water of both treatments. These findings provide new insights into the taxonomy and elevation-dependent toxicity of <i>Gyromitra</i> in the Southern Hemisphere. They also underscore the risks associated with the consumption of wild false morels and highlight the importance of informed and adequate culinary practices to reduce toxicity.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"682-694"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147817638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of the translation initiation factor eIF3j promotes the synthesis of amylase and kojic acid in the fungus <i>Aspergillus oryzae</i>.","authors":"Yuqing Gao, Yingqian Li, Shiying Wang, Siying Wu, Wenfeng Guo, Feng Zhang","doi":"10.1080/00275514.2026.2657612","DOIUrl":"10.1080/00275514.2026.2657612","url":null,"abstract":"<p><p>The edible fungus <i>Aspergillus oryzae</i> is well known for its powerful ability to secrete numerous proteolytic enzymes, such as lipases, proteases, and amylases. However, the mechanisms that regulate the translation of these enzymes in <i>A. oryzae</i> remain poorly understood. In this study, we investigated the biological function of the translation initiation factor eIF3j in <i>A. oryzae</i>. Our findings revealed that deletion of the <i>eIF3j</i> gene significantly inhibited mycelial growth and conidiation compared with the control strain. Furthermore, the <i>eIF3j</i> mutant showed increased sensitivity to various stressors relative to the control strain. Interestingly, the <i>eIF3j-</i>deleted strain exhibited enhanced production of amylase and kojic acid when compared with the control strain. Additional quantitative proteomic analysis suggested that eIF3j was involved in RNA processing, ribosome biogenesis, and amino acid metabolism. Taken together, these findings underscore the multifaceted role of eIF3j in <i>A. oryzae</i> and its potential as a target for improving traits relevant to industrial applications.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"718-731"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147931571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of bidirectional solid fermentation of <i>Irpex lacteus</i> and Chinese herbal medicines on alterations in antifungal activity.","authors":"Chengxin Huang, Huiyue Hu, Ying Zhang, Wang Shu, Yongxun Duan, Yueting Dai, Lingyun Liu, Changtian Li","doi":"10.1080/00275514.2026.2630619","DOIUrl":"10.1080/00275514.2026.2630619","url":null,"abstract":"<p><p>Bidirectional solid fermentation can augment the therapeutic efficacy of Chinese herbal medicines by increasing their bioactive constituents. Chinese herbal medicines demonstrate specific antifungal properties. The potential of bidirectional solid fermentation to augment the antifungal efficacy of Chinese herbal treatments against plant-pathogenic fungi remains unexamined. This study employed bidirectional solid fermentation utilizing <i>Irpex lacteus</i> and eight traditional Chinese herbal medicines to investigate alterations in antifungal efficacy. The findings indicated that <i>I. lacteus</i> had comparatively fast development on the majority of Chinese herbal medicine substrates. Bidirectional solid fermentation markedly improved antifungal efficacy across the majority of substrates. The ethanol extract of <i>Origanum vulgare</i>, fermented for 10 d, exhibited an inhibition rate against most plant-pathogenic fungi equivalent to that of carbendazim at the same dose. The correlation analysis indicated a favorable association between total terpenoid concentration and antifungal efficacy. The metabolomic investigation indicated that, relative to the unfermented control, the metabolites considerably elevated in <i>Origanum vulgare</i> after 10 d of fermentation were primarily terpenoids, including pseudolaric acid B and prednisolone acetate, whereas the significantly decreased metabolites were primarily shikimic acid and phenylpropanoids, such as salvianolic acid C and icariside I. The KEGG (Kyoto Encyclopedia of Genes and Genomes)  pathway enrichment analyses revealed that the most significantly enriched pathway was tyrosine metabolism. These findings will help facilitate the creation of prospective biocontrol agents, therefore offering sustainable alternatives for crop disease management.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"669-681"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A discussion on the genus <i>Stigmatomyces</i> sensu lato and the description of two new species from Chile.","authors":"Warre Van Caenegem, Cristian Montalva, Eladio Rojas, Paul L Th Beuk, André De Kesel, Annemieke Verbeken, Danny Haelewaters","doi":"10.1080/00275514.2026.2650075","DOIUrl":"10.1080/00275514.2026.2650075","url":null,"abstract":"<p><p>Taxonomic revisions in mycology often focus on well-studied fungal groups, yet obscure lineages such as Laboulbeniales (Pezizomycotina, Ascomycota) remain underexplored. Here, we address the taxonomy of <i>Stigmatomyces</i> sensu lato, a diverse genus of obligate biotrophic fungi parasitizing flies (Diptera). Previous work suggested that <i>Stigmatomyces</i> is paraphyletic, as the well-delineated genus <i>Gloeandromyces</i> was nested within the genus, which resulted in the reinstatement of <i>Appendiculina</i> and <i>Fanniomyces</i>. We reconstructed a molecular phylogeny of <i>Stigmatomyces</i> sensu lato using three genetic markers (18S and 28S nuc rDNA, <i>TEF1α</i>) and significantly expanded taxon sampling. Our results recovered six well-supported clades, including the three aforementioned genera, <i>Stigmatomyces</i> sensu stricto, and two unnamed lineages. Although the internal relationships among these clades remain unresolved, morphological distinctions further support their delimitation. We provide arguments both for and against splitting of the genus. A formal split of <i>Stigmatomyces</i> is not proposed due to still limited data and especially the lack of sequences from the type species, <i>S. baeri</i>. However, our findings are in support of the splitting of <i>Stigmatomyces</i> sensu lato in the future. Based on molecular phylogenetic, morphological, and ecological data, we also describe two new species from Chile, <i>Appendiculina drosophilae-huillicheae</i> on <i>Drosophila huilliche</i> (Drosophilidae) and <i>Stigmatomyces beukii</i> on <i>Prosopantrum</i> sp. (Cnemospathididae). Based on morphological, ecological, and/or molecular phylogenetic data, we propose four new combinations: <i>Appendiculina dacina, A. majewskii, Bordea euconni</i>, and <i>Sphaleromyces montevideensis</i>. Finally, we validly publish <i>Stigmatomyces biformis</i> and propose <i>Stigmatomyces rakiurensis</i> to accommodate <i>S. australis</i> M.B. Hughes, A. Weir, Gillen & W. Rossi, a later homonym of <i>S. australis</i> Thaxt.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"807-827"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and diversity of <i>Rhizopogon</i> species in Japan.","authors":"Hiroshi Abe, Hiromi Sasaki, Kazuhide Nara","doi":"10.1080/00275514.2026.2620129","DOIUrl":"10.1080/00275514.2026.2620129","url":null,"abstract":"<p><p><i>Rhizopogon</i> (Boletales) is an ectomycorrhizal (ECM) fungal genus that forms specific symbiotic associations with host trees within the family Pinaceae, playing a crucial role in seedling growth and establishment. Despite its ecological importance, the species diversity and phylogenetic relationships of <i>Rhizopogon</i> in East Asia remain poorly understood. Here, we investigate the diversity, phylogeny, and host specificity of Japanese <i>Rhizopogon</i> species. We conducted phylogenetic analyses of 106 basidiomata and three ECM root tip samples collected across Japan using three loci: the internal transcribed spacer (ITS), large subunit ribosomal RNA (LSU), and RNA polymerase II subunit B (<i>RPB2</i>). Phylogenetic analyses revealed at least 16 distinct lineages, including one only detected from a single root tip collected in Japan. Among the lineages with basidiomata, five corresponded to previously described species (<i>R. alpinus, R. boninensis, R. lucidus, R. togasawarius</i>, and <i>R. yakushimensis</i>). Seven other lineages, which did not cluster with any previously recognized species, are described here as new species and detailed morphological characteristics are provided: <i>R. citrinus, R. filivolubilis, R. flavoroseolus, R. japonicus, R. melliodorus, R. margaritaceus</i>, and <i>R. politus</i>. Two other lineages are new to Japan: <i>R. pseudoroseolus</i> and <i>R. evadens</i>. Another lineage, previously regarded as the Japanese \"Shouro,\" was reidentified as <i>R. graveolens</i> based on morphological and molecular evidence. This study is the first comprehensive taxonomic assessment of <i>Rhizopogon</i> in East Asia.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"759-788"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New manganese-oxidizing <i>Acremonium</i>-like fungi from halophytic rhizospheres in the Yellow River Delta, China.","authors":"Zhaoqi Yan, Yu Zhao, Xiaoyu Meng, Mengting Gao, Hongli Si, Guoyan Zhao, Tanay Bose, Runlei Chang","doi":"10.1080/00275514.2026.2627133","DOIUrl":"10.1080/00275514.2026.2627133","url":null,"abstract":"<p><p>Manganese (Mn) plays a vital role in soil chemistry, plant nutrition, and contaminant cycling, with certain microorganisms capable of transforming soluble Mn(II) into insoluble biogenic manganese oxides. Among these microorganisms, filamentous fungi are especially effective Mn oxidizers, often surpassing bacteria under challenging environmental conditions. The <i>Acremonium</i>-like fungi, historically recognized for their ecological versatility and production of bioactive metabolites, are now known to be highly polyphyletic, with species spanning multiple genera within the family Bionectriaceae. Despite their diversity, the ecological functions of these fungi remain poorly understood, and Mn-oxidizing activity has not previously been reported from this family. In this study, we surveyed saline-alkali soils in Dongying and Binzhou, Shandong Province, China. Seven <i>Acremonium</i>-like fungal isolates from halophytic rhizospheres were recovered from Dongying, but not from Binzhou. Multilocus phylogenetic analyses using ITS (internal transcribed spacer), 28S (28S ribosomal large subunit), <i>tef1-α</i> (elongation factor 1-alpha), and <i>rpb2</i> (second largest subunit of RNA polymerase II), along with detailed morphological characterization, demonstrated that these isolates represent three previously undescribed species within the genera <i>Acremonium, Protocreopsis</i>, and <i>Verruciconidia</i>. Remarkably, all isolates exhibited Mn(II)-oxidizing activity, representing the first report of this trait in Bionectriaceae. These findings broaden the taxonomic and ecological understanding of <i>Acremonium</i>-like fungi and suggest their potential role in manganese cycling in extreme soils. Their tolerance to environmental stress, combined with Mn-oxidizing capabilities, indicates potential applications in bioremediation, soil health enhancement, and biotechnology. By uncovering novel extremophilic fungi with functional significance, this study provides insights into microbial adaptation in harsh environments and identifies promising resources for the sustainable management of saline-alkali soils.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"695-707"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic identity of <i>Melampsora ferrinii</i> on <i>Salix</i> with <i>Melampsora coleosporioides</i>.","authors":"Taiga Kasuya, Yuriko Koide, Jing-Xin Ji, Izumi Okane, Yuichi Yamaoka, Tsuyoshi Hosoya, Makoto Kakishima","doi":"10.1080/00275514.2026.2619991","DOIUrl":"10.1080/00275514.2026.2619991","url":null,"abstract":"<p><p>Three willow rust fungi, <i>Melampsora babylonicae, M. coleosporioides</i>, and <i>M. ferrinii</i>, are morphologically similar and share comparable host associations. Morphological and phylogenetic analyses revealed that <i>M. coleosporioides</i> and <i>M. ferrinii</i> are taxonomically indistinguishable, whereas <i>M. babylonicae</i> is phylogenetically distinct and is therefore provisionally treated as a separate species. As a result, <i>M. ferrinii</i> is synonymized with <i>M. coleosporioides</i> and the description of <i>M. coleosporioides</i> is accordingly emended. The life cycle, host plant range, and geographic distribution of this rust fungus are described and discussed based on existing literature.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"749-758"},"PeriodicalIF":2.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}