Mycologia最新文献

{"title":"Pinibarreniales, a new order of Sordariomycetes from pine barrens ecosystem.","authors":"Jing Luo, Emily Walsh, Alexis Faulborn, Kevin Gao, James White, Ning Zhang","doi":"10.1080/00275514.2024.2363084","DOIUrl":"10.1080/00275514.2024.2363084","url":null,"abstract":"<p><p><i>Pinibarrenia chlamydospora</i>, sp. nov. isolated from the roots of highbush blueberry in the New Jersey Pine Barrens, is described and illustrated. Based on multigene phylogenetic analysis, as well as morphological and ecological characteristics, Pinibarreniales and Pinibarreniaceae are established to accommodate this novel lineage in Sordariomycetidae, Sordariomycetes. Pinibarreniales, Tracyllalales, and Vermiculariopsiellales are proposed to be included in the subclass Sordariomycetidae. Pinibarreniales likely have a wide distribution and forms association with Ericaceae plants that live in acidic and oligotrophic environments because its DNA barcode matches with environmental sequences from other independent ecological studies. The plant-fungal interaction experiment revealed negative impacts on <i>Arabidopsis</i>, indicating its pathogenicity. This uncovered new fungal lineage will contribute to a better understanding of the diversity and systematics of Sordariomycetes.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"835-847"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding host cell interaction- and fluconazole-induced metabolic alterations and drug resistance in <i>Candida auris</i>.","authors":"Samah H H Ismail, Rania Hamdy, Alaa M Altaie, Bahgat Fayed, Salam Dakalbab, Raafat El-Awady, Sameh S M Soliman","doi":"10.1080/00275514.2024.2363730","DOIUrl":"10.1080/00275514.2024.2363730","url":null,"abstract":"<p><p><i>Candida auris</i> is an emerging drug-resistant pathogen associated with high mortality rates. This study aimed to explore the metabolic alterations and associated pathogenesis and drug resistance in fluconazole-treated <i>Candida auris</i>-host cell interaction. Compared with controls, secreted metabolites from fluconazole-treated <i>C. auris</i> and fluconazole-treated <i>C. auris</i>-host cell co-culture demonstrated notable anti-<i>Candida</i> activity. Fluconazole caused significant reductions in <i>C. auris</i> cell numbers and aggregated phenotype. Metabolites produced by <i>C. auris</i> with potential fungal colonization, invasion, and host immune evasion effects were identified. Metabolites known to enhance biofilm formation produced during <i>C. auris</i>-host cell interaction were inhibited by fluconazole. Fluconazole enhanced the production of metabolites with biofilm inhibition activity, including behenyl alcohol and decanoic acid. Metabolites with potential <i>Candida</i> growth inhibition activity such as 2-palmitoyl glycerol, 1-tetradecanol, and 1-nonadecene were activated by fluconazole. Different patterns of proinflammatory cytokine expression presented due to fluconazole concentration and host cell type (fibroblasts versus macrophages). This highlights the immune response's complexity, emphasizing the necessity for additional research to comprehend cell-type-specific responses to antifungal therapies. Both host cell interaction and fluconazole treatment increased the expression of <i>CDR1</i> and <i>ERG11</i> genes, both associated with drug resistance. This study provides insights into pathogenesis in <i>C. auris</i> due to host cell interaction and fluconazole treatment. Understanding these interactions is crucial for enhancing fluconazole sensitivity and effectively combating <i>C. auris</i>.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"673-693"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141723953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revision of <i>Alternaria</i> sections <i>Pseudoulocladium</i> and <i>Ulocladioides</i>: Assessment of species boundaries, determination of mating-type loci, and identification of Russian strains.","authors":"Philipp B Gannibal, Maria M Gomzhina","doi":"10.1080/00275514.2024.2363152","DOIUrl":"10.1080/00275514.2024.2363152","url":null,"abstract":"<p><p><i>Alternaria</i> is a large genus within Pleosporaceae and consists of fungi that have up to recently been considered to be 15 separate genera, including <i>Ulocladium</i>. The majority of <i>Ulocladium</i> species after incorporation into <i>Alternaria</i> were placed in three sections: <i>Ulocladioides, Pseudoulocladium</i>, and <i>Ulocladium</i>. In this study, phylogeny of 26 reference strains of 22 species and 20 Russian <i>Ulocladium</i>-like isolates was recovered. The partial actin gene (<i>act</i>), <i>Alternaria</i> major allergen (<i>alta1</i>), calmodulin (<i>cal</i>), glyceraldehyde-3-phosphate dehydrogenase (<i>gapdh</i>), RNA polymerase II second largest subunit (<i>rpb2</i>), and translation elongation factor 1-α (<i>tef1</i>) were sequenced for Russian isolates. All these fungi were examined using multilocus phylogenetic analysis according to the genealogical concordance phylogenetic species recognition (GCPSR) principle and the coalescent-based model Poisson tree processes (PTP, mPTP) and evaluated for the presence of recombination. All strains were combined into two clades that corresponded to the <i>Pseudoulocladium</i> and <i>Ulocladioides</i> sections. The <i>Pseudoulocladium</i> clade included four reference strains and nine local isolates and considered to be a single species, whereas the <i>Ulocladioides</i> section comprises 11 species, instead of 17 names previously adopted. Nine species were abolished by joining four other species. Species <i>A. atra</i> and <i>A. multiformis</i> were combined into the single species <i>A. atra</i>. Five species, <i>A. brassicae-pekinensis, A. consortialis, A. cucurbitae, A. obovoidea</i>, and <i>A. terricola</i>, were united in the species <i>A. consortialis. Alternaria heterospora</i> and <i>A. subcucurbitae</i> were combined into one species, <i>A. subcucurbitae. Alternaria aspera, A. chartarum, A. concatenata</i>, and <i>A. septospora</i> were combined into a single species, <i>A. chartarum</i>. Also, amplification with two different primer sets was performed to define mating-type locus 1 (<i>MAT1</i>) idiomorph. All studied isolates were heterothallic, contradicting some prior studies. Twenty Russian <i>Ulocladium</i>-like isolates were assigned to five species of two sections, <i>A. atra, A. cantlous, A. chartarum, A. consortialis</i>, and <i>A. subcucurbitae</i>. Species <i>A. cantlous</i> and <i>A. subcucurbitae</i> were found in Russia for the first time.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"744-763"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141723955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ophiostoma haidaense</i>, sp. nov., a new member of the <i>O. piceae</i> species complex associated with yellow-cedar, <i>Callitropsis nootkatensis</i>.","authors":"Nicolas Feau, Joey B Tanney, Padmini Herath, Stefan Zeglen, Richard C Hamelin","doi":"10.1080/00275514.2024.2357968","DOIUrl":"10.1080/00275514.2024.2357968","url":null,"abstract":"<p><p><i>Ophiostoma haidanensis</i> is described as a new species of the <i>Ophiostoma piceae</i> complex isolated from yellow-cedar (<i>Callitropsis nootkatensis</i> (D. Don) Oerst. ex D.P. Little) sapwood in the Haida Gwaii island archipelago and the North Coast of British Columbia, Canada. The fungus is characterized by the production of a typical sporothrix-like asexual morph but is distinguished morphologically from other members of the <i>O. piceae</i> species complex by its large, multiseptate primary conidia. Phylogenetic analysis of DNA sequences from the nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS) and the β-tubulin (<i>BTUB</i>) and translation elongation factor 1-α (<i>TEF1</i>) genes supports the inclusion of <i>O. haidensis</i> as a distinct member within the <i>O. piceae</i> complex. To our knowledge, this is the first report of a blue stain fungus infecting yellow-cedar, an ecologically, culturally, and economically important conifer naturally distributed along the coastal forests of the Pacific Northwest in North America.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"694-707"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downfall of an empire: Unmasking the hidden diversity and distribution of the <i>Amanita rubescens</i> species complex.","authors":"Christian A Quintero-Corrales, Melania Vega, Amaranta Ramírez-Terrazo, Bernardo Águila, Roberto Garibay-Orijel","doi":"10.1080/00275514.2024.2355276","DOIUrl":"10.1080/00275514.2024.2355276","url":null,"abstract":"<p><p><i>Amanita</i> is one of the most salient mushroom genera due to its cultural, economic, and medical importance. Recently, many new <i>Amanita</i> species have been described worldwide, increasing the genus richness. However, several clades have cryptic diversity, and many species complexes have not yet been resolved. This is the case of the rubescent species in the <i>Validae</i> section, which have been widely cited under the name <i>Amanita rubescens</i> s.l. We used a four-locus matrix (nuc rDNA internal transcribed spacer [ITS] and 28S regions and genes for RNA polymerase II subunit 2 [<i>rpb2</i>], translation elongation factor 1-α [<i>tef1-α</i>], and β-tubulin [<i>tub2</i>]) to solve the phylogenetic relationships within the <i>Amanita</i> section <i>Validae</i>. To analyze the diversity and distribution patterns of species, we used an extensive ITS sequence sampling including environmental DNA databases. The phylogenetic analyses demonstrated that the <i>Validae</i> section is divided into three monophyletic and highly supported major clades: <i>Mappae, Validae</i>, and <i>Rubescentes</i>. At least 11 species-level clades within the <i>Rubescentes</i> clade were highly supported: <i>A. cruentilemurum</i> nom. prov. <i>A. brunneolocularis, A. rubescens</i> s.s. (European clade), <i>A. rubescens</i> s.s. (Asiatic clade), <i>A. orsonii</i> s.s. <i>A</i>. '<i>orsonii</i>,' <i>A. aureosubucula</i> nom. prov., <i>A. novinupta, A. flavorubens</i>, and two undescribed North American species. We proved that <i>A. rubescens</i> s.s. has two segregated populations (European and Asiatic) and it is not naturally distributed in America. Furthermore, we found that America has more cryptic species within the <i>Rubescentes</i> clade than Eurasia.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"642-649"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new and threatened species of <i>Bondarzewia</i> from the Brazilian cloud forests.","authors":"Carlos A Salvador-Montoya, Genivaldo Alves-Silva, Thiago Kossmann, Felipe Bittencourt, Daniela Werner, Kelmer Martins-Cunha, Orlando F Popoff, Aristóteles Góes-Neto, Mario Rajchenberg, Elisandro R Drechsler-Santos","doi":"10.1080/00275514.2024.2355337","DOIUrl":"10.1080/00275514.2024.2355337","url":null,"abstract":"<p><p>A new and threatened polypore species, <i>Bondarzewia loguerciae</i>, is described from the cloud forests of southern Brazil. It is characterized by single-pileate basidiomata that grow on dead branches and along living stems of standing trunks and present a context with dark lines and resinous tubes. When growing in axenic culture, this species also develops chlamydospores. We provide an illustrated morphological description and molecular analysis. Our specimens from Brazil form a monophyletic group among other species of the Southern Hemisphere. The conservation status of <i>B. loguerciae</i> is assessed and published as \"Critically Endangered\" based on the International Union for Conservation of Nature  (IUCN) criteria. Additionally, a key to the species is provided.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"775-791"},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Cortinariaceae species associated with Dicymbe, Aldina, and Pakaraimaea in Guyana","authors":"Noah Siegel, Terry W. Henkel, Shannon Adams, Jerry Cooper, M. Catherine Aime","doi":"10.1080/00275514.2024.2367399","DOIUrl":"https://doi.org/10.1080/00275514.2024.2367399","url":null,"abstract":"Species of the ectomycorrhizal (ECM) family Cortinariaceae (Agaricales, Agaricomycetes, Basidiomycota) have long been considered impoverished or absent from lowland tropical rainforests. Several de...","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"55 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141587827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In memoriam: Lorelei Louise Norvell, September 6, 1943-August 4, 2023.","authors":"Joe Ammirati, Scott A Redhead","doi":"10.1080/00275514.2024.2341442","DOIUrl":"https://doi.org/10.1080/00275514.2024.2341442","url":null,"abstract":"","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"116 4","pages":"625-627"},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new fossil species of <i>Meliolinites</i> Selkirk associated with <i>Rhodoleia</i> leaves from the Upper Pliocene of southwestern China.","authors":"Zhuo-Er Wang, Zhi-Hui Song, Rui Cao, Han-Shi Li, Gui-Hua Chen, Su-Ting Ding, Jing-Yu Wu","doi":"10.1080/00275514.2024.2348980","DOIUrl":"10.1080/00275514.2024.2348980","url":null,"abstract":"<p><p>Fossil epifoliar fungi are valuable indicators of paleoenvironment and paleoecology. The Meliolaceae, members of which typically inhabit the surface of living plants as biotrophs or pathogens, is one of the largest groups of epifoliar fungi. In this study, we report a novel fossil species of <i>Meliolinites</i> Selkirk (fossil Meliolaceae), <i>Meliolinites tengchongensis</i>, on the lower epidermis of compressed fossil <i>Rhodoleia</i> (Hamamelidaceae) leaves from the Upper Pliocene Mangbang Formation of Tengchong, Yunnan, southwestern China. <i>Meliolinites tengchongensis</i> is characterized by web-like, superficial, brown to dark brown, septate, and branching mycelia bearing 2-celled appressoria and unicellular phialides. The fungal colonies also include ellipsoidal, 5-celled, 4-septate ascospores and dark brown perithecia with suborbicular outline and verrucose surface. The well-preserved vegetative and reproductive organs help us to explore the potential disease process of the new fossil species. Besides, the presence of fungal remains indicates that the fungal taxon might have maintained its host preference since at least the Late Pliocene. Furthermore, the occurrence of both fossil fungi and their host plants in Tengchong indicate a subtropical-tropical, warm, and humid climate during the Late Pliocene, whereas the distribution pattern of the fungi on the host leaves suggests that <i>Rhodoleia</i> may have been a part of the middle-upper canopies in the Tengchong Late Pliocene multilayered forest.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"498-508"},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetics of the rust fungi (Pucciniales) of South Africa, with notes on their life histories and possible origins.","authors":"Alan R Wood, M Catherine Aime","doi":"10.1080/00275514.2024.2334189","DOIUrl":"10.1080/00275514.2024.2334189","url":null,"abstract":"<p><p>South Africa has an indigenous rust (Pucciniales) funga of approximately 460 species. This funga was sampled with species from as many genera as possible. The nuclear ribosomal large subunit (28S) region was amplified from samples representing 110 indigenous species, as well as the small subunit (18S) region and the cytochrome <i>c</i> oxidase subunit 3 (CO3) in some cases, and these were used in phylogenetic analyses. One new species is described, 12 new combinations made, six names reinstated, and two life history connections made. The life histories of this funga were summarized; it is dominated by species with contracted life histories. The majority of species are autoecious, with a small proportion being heteroecious. Of the autoecious species, many will likely be homothallic with no spermagonia. A shortened life history with homothallism allows for a single basidiospore infection to initiate a local population buildup under the prevailing unpredictable climatic conditions. Suggestions are made as to the possible origin of this funga based on the development of the modern South African flora. It is postulated that the rusts of South Africa are of relatively recent origin, consisting of three groups. Firstly, there is an African tropical element with members of the Mikronegerineae (<i>Hemileia</i>), the Sphaerophragmiaceae (<i>Puccorchidium, Sphaerophragmium</i>), and certain Uredinineae (<i>Stomatisora</i>). Their immediate ancestors likely occurred in the tropical forests of Africa during the Paleogene. Secondly, there is a pantropical element including the Raveneliaceae (e.g., <i>Diorchidium, Maravalia, Ravenelia</i> sensu lato, <i>Uropyxis</i>). This likely diversified during the Neogene, when the mimosoids became the dominant trees of the developing savannas. Thirdly, the Pucciniaceae invaded Africa as this continent pushed northward closing the Tethys Sea. They diversified with the development of the savannas as these become the dominant habitat in most of Africa, and are by far the largest component of the South African rust funga.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"509-535"},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}