Molecular Biology最新文献_第7页

Genome Stability of Bacillus velezensis after Two-Year Exposure in Open Space 露天暴露两年后 Velezensis 杆菌基因组的稳定性

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010023

S. V. Fialkina, E. A. Deshevaya, A. L. Rakitin, O. I. Orlov

{"title":"Genome Stability of Bacillus velezensis after Two-Year Exposure in Open Space","authors":"S. V. Fialkina, E. A. Deshevaya, A. L. Rakitin, O. I. Orlov","doi":"10.1134/s0026893324010023","DOIUrl":"https://doi.org/10.1134/s0026893324010023","url":null,"abstract":"Abstract—Spore-forming bacteria have a unique resistance to negative environmental conditions, including aggressive space factors, and are an excellent model for studying adaptation mechanisms and survival strategies at the molecular level. The study analyzed the genome of Bacillus velezensis, which remained viable after a 2-year exposure in outer space on the outer surface of the ISS as part of the Test space experiment. A comparative analysis of the draft genomes of the exhibit strain and the ground control did not reveal significant changes; the average nucleotide identity was 99.98%, which indicates the ability of microorganisms to maintain genome stability in space conditions, due to both increased stress resistance of bacterial spores and efficient operation of the system of repair of accumulated changes. The study of a single nucleotide polymorphism in the genome of B. velezensis revealed nine point substitutions, three of which are in intergenic regions, six in protein-coding genes, three of them are missense mutations, two nucleotide deletions leading to a shift in the reading frame, and one synonymous substitution. The profiles of the housekeeping genes were determined during MLST typing and it was found that the allelic profiles obtained for B. velezensis T15.2 and 924 strains do not correspond to any of the previously described sequence types.The presented results indicate the ability of B. velezensis bacteria to maintain the viability of spores and the integrity of the genome for a long time under extreme conditions of outer space, which is important for the problem of planetary protection, as well as the potential possibility of performing biotechnological processes based on B. velezensis during space exploration.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"278 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin Enhances the Effect of ABT-737 in Acute Monocytic Leukemia THP-1 Cells 褪黑激素能增强 ABT-737 对急性单核细胞白血病 THP-1 细胞的作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-02-01 DOI: 10.1134/s002689332401014x

{"title":"Melatonin Enhances the Effect of ABT-737 in Acute Monocytic Leukemia THP-1 Cells","authors":"","doi":"10.1134/s002689332401014x","DOIUrl":"https://doi.org/10.1134/s002689332401014x","url":null,"abstract":" <h3>Abstract</h3> Melatonin (N-acetyl-5-methoxytryptamine, MEL) is a hormone synthesized by the pineal gland. Due to its oncostatic effect, it can be considered as an antitumor agent and used for combination therapy. ABT-737, a Bcl-2 inhibitor, promotes cell death after treatment with agents that induce pro-apoptotic signals. In the present study, the combined effect of MEL and ABT-737 on changes in proliferative and mitotic activity, mitochondrial membrane potential, intracellular production of reactive oxygen species (ROS), and cytosolic Ca2+ was studied. Moreover, changes in the expression of anti- and pro-apoptotic proteins (Bcl-2 and Bax), autophagy markers (LC3A/B (I, II)), endoplasmic reticulum stress markers (chaperones BIP and PDI, CHOP) were studied under these conditions. The effect of MEL together with ABT-737 led to an increase in the level of cytosolic Ca2+, intracellular production of ROS and a decrease in the membrane potential of mitochondria. The content of Bcl-2 increased, while the level of Bax decreased. Activation of CHOP stimulated autophagy and led to a decrease in the synthesis of chaperones BIP and PDI. It is assumed that melatonin can enhance the effect of other chemotherapeutic agents and can be used in the treatment of tumors. ","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"79 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stochastic Packaging of Cas Proteins into Exosomes 将 Cas 蛋白随机包装到外泌体中

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-02-01 DOI: 10.1134/s0026893324010102

{"title":"Stochastic Packaging of Cas Proteins into Exosomes","authors":"","doi":"10.1134/s0026893324010102","DOIUrl":"https://doi.org/10.1134/s0026893324010102","url":null,"abstract":"Abstract—CRISPR/Cas systems are perspective molecular tools for targeted manipulation with genetic materials, such as gene editing, regulation of gene transcription, modification of epigenome etc. While CRISPR/Cas systems proved to be highly effective for correcting genetic disorders and treating infectious diseases and cancers in experimental settings, clinical translation of these results is hampered by the lack of efficient CRISPR/Cas delivery vehicles. Modern synthetic nanovehicles based on organic and inorganic polymers have many disadvantages, including toxicity issues, the lack of targeted delivery, and complex and expensive production pipelines. In turn, exosomes are secreted biological nanoparticles that exhibit high biocompatibility, physico-chemical stability, and the ability to cross biological barriers. Early clinical trials found no toxicity associated with exosome injections. In the recent years, exosomes have been considered as perspective delivery vehicles for CRISPR/Cas systems in vivo. The aim of this study was to analyze the efficacy of CRISPR/Cas stochastic packaging into exosomes for several human cell lines. Here, we show that Cas9 protein is effectively localized into the compartment of intracellular exosome biogenesis, but stochastic packaging of Cas9 into exosomes turns to be very low (~1%). As such, stochastic packaging of Cas9 protein is very ineffective and cannot be used for gene editing purposes. Developing novel tools and technologies for loading CRISPR/Cas systems into exosomes is needed.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"278 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restraint Stress-Induced Expression of Fos and Several Related Genes in the Hypothalamus of Hypertensive ISIAH Rats 约束应激诱导高血压 ISIAH 大鼠下丘脑中 Fos 和几个相关基因的表达

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-02-01 DOI: 10.1134/s0026893324010072

{"title":"Restraint Stress-Induced Expression of Fos and Several Related Genes in the Hypothalamus of Hypertensive ISIAH Rats","authors":"","doi":"10.1134/s0026893324010072","DOIUrl":"https://doi.org/10.1134/s0026893324010072","url":null,"abstract":" <h3>Abstract</h3> Stress can play a significant role in arterial hypertension and many other complications of cardiovascular diseases. Considerable attention is paid to the study of the molecular mechanisms involved in the body response to stressful influences, but there are still many blank spots in understanding the details. ISIAH rats model the stress-sensitive form of arterial hypertension. ISIAH rats are characterized by genetically determined enhanced activities of the hypothalamic–pituitary–adrenocortical and sympathetic–adrenomedullary systems, suggesting a functional state of increased stress reactivity. For the first time, the temporal expression patterns of Fos and several related genes were studied in the hypothalamus of adult male hypertensive ISIAH rats after a single exposure to restraint stress for 30, 60, or 120 min. Fos transcription was activated and peaked 1 h after the start of restraint stress. The time course of Fos activation coincided with that of blood pressure increase after stress. Activation of hypothalamic neurons also alters the transcription levels of several transcription factor genes (Jun, Nr4a3, Jdp2, and Ppargc1a), which are associated with the development of cardiovascular diseases. Because Fos induction is a marker of brain neuron activation, activation of hypothalamic neurons and an increase in blood pressure were concluded to accompany increased stress reactivity of the hypothalamic–pituitary–adrenocortical and sympathoadrenal systems in hypertensive ISIAH rats during short-term restraint. ","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"56 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling Protein–Protein Interaction of the KNOTTED-LIKE HOMEOBOX 3 Protein Involved in Symbiotic Nodule Development in Medicago truncatula 参与美智子共生结节发育的 KNOTTED-LIKE HOMEOBOX 3 蛋白的蛋白质相互作用建模

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-01-21 DOI: 10.1134/s0026893324020031

M. Azarakhsh, M. Lebedeva, V. K. Vishvakarma

{"title":"Modeling Protein–Protein Interaction of the KNOTTED-LIKE HOMEOBOX 3 Protein Involved in Symbiotic Nodule Development in Medicago truncatula","authors":"M. Azarakhsh, M. Lebedeva, V. K. Vishvakarma","doi":"10.1134/s0026893324020031","DOIUrl":"https://doi.org/10.1134/s0026893324020031","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>KNOTTED-LIKE HOMEOBOX (KNOX) proteins are homeodomain containing transcription factors, and regulate many aspects of plant development through homo or heterodimerization with another group of TALE transcription factors known as BELL. In Medicago truncatula the MtKNOX3 gene is involved in nodule development. In this work, we hypothesized that MtKNOX3 involvement in the activation of cytokinin signaling during nodule development could be through heterodimerization with BELL proteins. Thereby, the expression of different BELL genes in Medicago was analyzed, and it was shown that the expression of Medtr8g078480 and Medtr8g098815 genes increases during nodule development. Besides, the Medtr8g078480 shows a co-expression pattern with MtKNOX3 at different developmental stages of nodule development. Afterward, the interaction of MtKNOX3 with the MtBELL1-2 (Medtr8g078480) protein was shown using docking, and their stability was analyzed by molecular dynamic simulation and mmpbsa methods. Moreover, the stability of MtKNOX3- Medtr8g078480 heterodimers was compared with the MtKNOX3 homodimer.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"267 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139561577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ischemia–Reperfusion Injury: Molecular Mechanisms of Pathogenesis and Methods of Their Correction 缺血再灌注损伤：发病的分子机制及其纠正方法

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060067

R. G. Goncharov, M. G. Sharapov

引用次数: 0

Long Noncoding RNAs MEG3, TUG1, and hsa-miR-21-3p Are Potential Diagnostic Biomarkers for Coronary Artery Disease 长非编码 RNA MEG3、TUG1 和 hsa-miR-21-3p 是冠状动脉疾病的潜在诊断生物标记物

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893324010126

M. Abdelgawad, H. Y. Abdallah, A. Fareed, A. E. Ahmed

{"title":"Long Noncoding RNAs MEG3, TUG1, and hsa-miR-21-3p Are Potential Diagnostic Biomarkers for Coronary Artery Disease","authors":"M. Abdelgawad, H. Y. Abdallah, A. Fareed, A. E. Ahmed","doi":"10.1134/s0026893324010126","DOIUrl":"https://doi.org/10.1134/s0026893324010126","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Peripheral blood biomarkers are of particular importance to diagnose certain diseases including coronary artery disease (CAD) due to their non-invasiveness. Investigating the expression of noncoding RNAs (ncRNAs) paves the way to early disease diagnosis, prognosis, and treatment. Consequently, in this research, we aimed to investigate a panel of ncRNAs as potential biomarkers in patients with coronary artery disease. Two different groups have been designed (control and CAD). All participants were subjected to interviews and clinical examinations. Peripheral blood samples were collected, and plasma was extracted. At the same time, target ncRNAs have been selected based on literature review and bioinformatic analysis, and later they underwent investigation using quantitative real-time PCR. The selected panel encompassed the long non-coding RNAs (lncRNAs) MEG3, TUG1, and SRA1, and one related microRNA (miRNA): hsa-miR-21-3p. We observed statistically significant upregulation in MEG3, TUG1, and hsa-miR21-3p in CAD patients compared to control participants (p-value < 0.01). Nevertheless, SRA1 exhibited downregulation with no statistical significance (p-value > 0.05). All ncRNAs under study displayed a significantly strong correlation with disease incidence, age, and smoking. Network construction revealed a strong relationship between MEG3 and TUG1. ROC analysis indicated high potentiality for hsa-miR-21-3p to be a promising biomarker for CAD. Moreover, MEG3 and TUG1 displayed distinguished diagnostic discrimination but less than hsa-miR-21-3p, all of them exhibited strong statistical significance differences between CAD and control groups. Conclusively, this research pinpointed that MEG3, TUG1, and hsa-miR-21-3p are potential biomarkers of CAD incidence and diagnosis.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"5 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Repeats Show Clade-Specific Volatility in Aves 蛋白重复序列在鸟类中显示出特定支系的波动性

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893324010163

S. Sharma, L. Teekas, N. Vijay

{"title":"Protein Repeats Show Clade-Specific Volatility in Aves","authors":"S. Sharma, L. Teekas, N. Vijay","doi":"10.1134/s0026893324010163","DOIUrl":"https://doi.org/10.1134/s0026893324010163","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Protein repeats are a source of rapid evolutionary and functional novelty. Repeats are crucial in development, neurogenesis, immunity, and disease. Repeat length variability and purity can alter the outcome of a pathway by altering the protein structure and affecting the protein−protein interaction affinity. Such rampant alterations can facilitate species to rapidly adapt to new environments or acquire various morphological/physiological features. With more than 11000 species, the avian clade is one of the most speciose vertebrate clades, with near-ubiquitous distribution globally. Explosive adaptive radiation and functional diversification facilitated the birds to occupy various habitats. High diversity in morphology, physiology, flight pattern, behavior, coloration, and life histories make birds ideal for studying protein repeats’ role in evolutionary novelty. Our results demonstrate a similar repeat diversity and proportion of repeats across all the avian orders considered, implying an essential role of repeats in necessary pathways. We detected positively selected sites (PSS) in the polyQ repeat of RUNX2 in the avian clade; and considerable repeat length contraction in the Psittacopasserae. The repeats show a species-wide bias towards a contraction in Galloanseriformes. Interestingly, we detected the length contrast of polyS repeat in PCDH20 between Galliformes and Anseriformes. We speculate the length variability of serine repeat and its interaction with β-catenin in the Wnt/β-catenin signaling pathway could have facilitated fowls to adapt to their respective environmental conditions. We believe our study emphasizes the role of protein repeats in functional/morphological diversification in birds. We also provide an extensive list of genes with considerable repeat length contrast to further explore the role of length volatility in evolutionary novelty and rapid functional diversification.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"165 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of SENP6 with PINK1 Promotes Temozolomide Resistance in Neuroglioma Cells via Inducing the Mitophagy SENP6 与 PINK1 的相互作用通过诱导有丝分裂促进神经胶质瘤细胞对替莫唑胺的抗性

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893324010175

Y. W. Wang, K. G. Jia, H. J. Xing, Y. Pan, C. S. Zeng, L. Chen, Q. J. Su, W. T. Shen, J. Chen, C. Chen, Q. Cao, Y. Y. Wang

{"title":"Interaction of SENP6 with PINK1 Promotes Temozolomide Resistance in Neuroglioma Cells via Inducing the Mitophagy","authors":"Y. W. Wang, K. G. Jia, H. J. Xing, Y. Pan, C. S. Zeng, L. Chen, Q. J. Su, W. T. Shen, J. Chen, C. Chen, Q. Cao, Y. Y. Wang","doi":"10.1134/s0026893324010175","DOIUrl":"https://doi.org/10.1134/s0026893324010175","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Temozolomide resistance is a major cause of recurrence and poor prognosis in neuroglioma. Recently, growing evidence has suggested that mitophagy is involved in drug resistance in various tumor types. However, the role and molecular mechanisms of mitophagy in temozolomide resistance in glioma remain unclear. In this study, mitophagy levels in temozolomide-resistant and -sensitive cell lines were evaluated. The mechanisms underlying the regulation of mitophagy were explored through RNA sequencing, and the roles of differentially expressed genes in mitophagy and temozolomide resistance were investigated. We found that mitophagy promotes temozolomide resistance in glioma. Specifically, small ubiquitin-like modifier specific protease 6 (SENP6) promoted temozolomide resistance in glioma by inducing mitophagy. Protein-protein interactions between SENP6 and the mitophagy executive protein PTEN-induced kinase 1 (PINK1) resulted in a reduction in small ubiquitin-like modifier 2 (SUMO2)ylation of PINK1, thereby enhancing mitophagy. Our study demonstrates that by inducing mitophagy, the interaction of SENP6 with PINK1 promotes temozolomide resistance in glioblastoma. Therefore, targeting SENP6 or directly regulating mitophagy could be a potential and novel therapeutic target for reversing temozolomide resistance in glioma.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"23 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protective Action of Hsp70 and Hydrogen Sulfide Donors in THP-1 Macrophages in the Lipopolysaccharide-Induced Inflammatory Response by Modulating Endocytosis Hsp70 和硫化氢捐献者通过调节内吞作用保护 THP-1 巨噬细胞在脂多糖诱导的炎症反应中的作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060213

M. M. Yurinskaya, D. G. Garbuz, M. B. Evgen’ev, M. G. Vinokurov

{"title":"The Protective Action of Hsp70 and Hydrogen Sulfide Donors in THP-1 Macrophages in the Lipopolysaccharide-Induced Inflammatory Response by Modulating Endocytosis","authors":"M. M. Yurinskaya, D. G. Garbuz, M. B. Evgen’ev, M. G. Vinokurov","doi":"10.1134/s0026893323060213","DOIUrl":"https://doi.org/10.1134/s0026893323060213","url":null,"abstract":"Abstract—Hsp70 and hydrogen sulfide donors reduce inflammatory processes in human and animal cells. The biological action mediated by Hsp70 and H2S donors (GYY4137 and sodium thiosulfate) depends on their protection kinetics from cell activation by lipopolysaccharides. However, the molecular mechanisms of action of Hsp70 and H2S are not well understood. We studied the effect of human recombinant Hsp70 and H2S donors on the formation of reactive oxygen species and tumor necrosis factor-alpha induced in human cells (THP-1) by lipopolysaccharides. Transcriptomic changes occurring in these cells after LPS administration in combination with GYY4137 pretreatment were investigated. The results we obtained showed that Hsp70 and hydrogen sulfide donors reduce inflammatory processes in cells activated by the action of LPS. Hsp70 and H2S donors differed in the kinetics of the protective action, while hydrogen sulfide donors turned out to be more effective. The role of endocytosis in the mechanisms of protection of cells by H2S and Hsp70 donors from the action of LPS was studied. It has been found that GYY4137 pretreatment of LPS-exposed cells reduces the LPS-induced induction of various pro-inflammatory genes and affects the expression of genes of various intracellular signaling pathways.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138555977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0