Molecular Biology最新文献_第7页

The Structural Features of Skeletal Muscle Titin Aggregates 骨骼肌 Titin 聚集体的结构特征

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020043

L. G. Bobyleva, T. A. Uryupina, N. V. Penkov, M. A. Timchenko, A. D. Ulanova, A. G. Gabdulkhakov, I. M. Vikhlyantsev, A. G. Bobylev

{"title":"The Structural Features of Skeletal Muscle Titin Aggregates","authors":"L. G. Bobyleva, T. A. Uryupina, N. V. Penkov, M. A. Timchenko, A. D. Ulanova, A. G. Gabdulkhakov, I. M. Vikhlyantsev, A. G. Bobylev","doi":"10.1134/s0026893324020043","DOIUrl":"https://doi.org/10.1134/s0026893324020043","url":null,"abstract":"<p><b>Abstract</b>—Titin is a multidomain protein of striated and smooth muscles of vertebrates. The protein consists of repeating immunoglobulin-like (Ig) and fibronectin-like (FnIII) domains, which are β-sandwiches with a predominant β-structure, and also contains disordered regions. In this work, the methods of atomic force microscopy (AFM), X-ray diffraction, and Fourier transform infrared spectroscopy were used to study the morphology and structure of aggregates of rabbit skeletal muscle titin obtained in two different solutions: 0.15 M glycine-KOH, pH 7.0 and 200 mM KCl, 10 mM imidazole, pH 7.0. According to AFM data, skeletal muscle titin formed amorphous aggregates of different morphologies in the above two solutions. Amorphous aggregates of titin formed in a solution containing glycine consisted of much larger particles than aggregates of this protein formed in a solution containing KCl. The “KCl-aggregates” according to AFM data had the form of a “sponge”-like structure, while amorphous “glycine-aggregates” of titin formed “branching” structures. Spectrofluorometry revealed the ability of “glycine-aggregates” of titin to bind to the dye thioflavin T (TT), and X-ray diffraction revealed the presence of one of the elements of the amyloid cross β-structure, a reflection of ~4.6 Å, in these aggregates. These data indicate that “glycine-aggregates” of titin are amyloid or amyloid-like. No similar structural features were found in “KCl-aggregates” of titin; they also did not show the ability to bind to thioflavin T, indicating the non-amyloid nature of these titin aggregates. Fourier transform infrared spectroscopy revealed differences in the secondary structure of the two types of titin aggregates. The data we obtained demonstrate the features of structural changes during the formation of intermolecular bonds between molecules of the giant titin protein during its aggregation. The data expand the understanding of the process of amyloid protein aggregation.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"44 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uveal Melanoma: Molecular and Genetic Mechanisms of Development and Therapeutic Approaches 葡萄膜黑色素瘤：发展的分子和遗传机制及治疗方法

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020183

M. V. Zhilnikova, O. S. Troitskaya, D. D. Novak, V. V. Atamanov, O. A. Koval

{"title":"Uveal Melanoma: Molecular and Genetic Mechanisms of Development and Therapeutic Approaches","authors":"M. V. Zhilnikova, O. S. Troitskaya, D. D. Novak, V. V. Atamanov, O. A. Koval","doi":"10.1134/s0026893324020183","DOIUrl":"https://doi.org/10.1134/s0026893324020183","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Uveal melanoma (UM) is a neuroectodermal tumor that results from malignant transformation of melanocytes in the eye uvea, including the iris, the ciliary body, and the choroid. UM accounts for 5% of all melanoma cases and is extremely aggressive with half of the UM patients developing metastases within the first 1‒2 years after tumor development. Molecular mechanisms of UM carcinogenesis are poorly understood, but are known to differ from those of skin melanoma. Activating mutations of the <i>GNAQ</i> and <i>GNA11</i> genes, which code for the large G protein subunits Gq and G11, respectively, are found in 90% of UM patients. The Gaq/PKC/MAPK signaling pathway is a main signaling cascade that leads to the transformation of melanocytes of the uveal tract, and major regulators of the cascade provide targets for the development of drugs. Metastatic UM (MUM) is most often associated with mutations of <i>BAP1</i>, <i>EIF1AX</i>, <i>GNA11</i>, <i>GNAQ</i>, and <i>SF3B1</i>. A combination of a commercial expression test panel of 15 genes and a mutation panel of 7 genes, supplemented with data on the size of the primary tumor, is highly efficient in predicting the risk of metastasis. The risk of metastasis determines the choice of therapy and the patient follow-up regimen. However, no systemic therapy for MUM has been developed to date. New drugs undergoing clinical trials are mostly targeted drugs designed to inhibit the protein products of mutant genes or immunotherapeutic agents designed to stimulate the immune response against specific antigens. In addition to these approaches, potential therapeutic targets of epigenetic regulation of UM development are considered in the review.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"45 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What Actin and Myosin Do in the Nucleus: New Functions of the Well-Known Proteins 肌动蛋白和肌球蛋白在细胞核中的作用：著名蛋白质的新功能

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s002689332470002x

A. A. Saidova, I. A. Vorobjev

引用次数: 0

Revision of Functionally Relevant and Widely Expressed Long Non-Coding RNAs 修订功能相关且广泛表达的长非编码 RNA

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700183

D. Konina, M. Skoblov

{"title":"Revision of Functionally Relevant and Widely Expressed Long Non-Coding RNAs","authors":"D. Konina, M. Skoblov","doi":"10.1134/s0026893324700183","DOIUrl":"https://doi.org/10.1134/s0026893324700183","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Long non-coding RNAs (lncRNAs) are involved in many cellular processes while displaying high tissue specificity. In contrast, protein-coding genes, including the category of housekeeping ones, exhibit broad expression patterns. The aim of this study was to highlight the functional importance of widely expressed lncRNAs. We analyzed experimental data from cell-growth screen of lncRNA loci in human cells, which allowed us to identify 18 lncRNA hits. Notably, these lncRNAs were not only widely expressed in most human tissues, but also played functional roles within them. Detail investigation revealed them encompass a variety of molecular functions, from cardiomyocyte damage controlling to macrophage class switching. Interestingly, experimental data highlighted the fact that a significant part of these lncRNAs encoded small but functional polypeptides. A set of lncRNAs, NEAT1, SNHG1, SNHG7, SNHG12, SNHG15, SNHG16, MIR17HG, LINC00680, LINC00263 and LINC00339, that were highly likely to be translated into small polypeptides was identified. Additionally, for EPB41L4A-AS1, CRNDE, SNHG6, LINC00493, and LINC01420, a dual function associated with both the RNA sequences and small proteins they encoded was established.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"38 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease Mouse Model 钙粘连蛋白 cRNA 探针在阿尔茨海默病小鼠模型大脑中的应用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700134

H. Zhou, S. J. Du, F. Gendi, H. Y. Li, J. Fu, C. Chang

{"title":"Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease Mouse Model","authors":"H. Zhou, S. J. Du, F. Gendi, H. Y. Li, J. Fu, C. Chang","doi":"10.1134/s0026893324700134","DOIUrl":"https://doi.org/10.1134/s0026893324700134","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The cadherin superfamily molecules, functioning as cell adhesion molecules, are recognized to play roles in both physiological and pathological processes. The cadherin-based adherent junction (CAJ) is believed to interact with presenilin-1 (PS-1), suggesting that disruptions in CAJ structures might contribute to neurodegeneration, potentially leading to Alzheimer’s Disease (AD). Yet, the specific expression patterns of cadherin superfamily mRNA remain somewhat ambiguous. This research utilized in situ hybridization (ISH) to examine the expression and localization of cadherin mRNA in AD mouse model brains. Long cRNA probes targeting cadherin revealed endogenous mRNA expression in brain sections. Interestingly, senile plaques in the AD mouse brain were also bound to these probes. This binding, however, did not exclusively denote cadherin mRNA, as ISH detected both antisense and sense cRNA probes. Our data suggest that although antisense cRNA probes effectively detected cadherin mRNA expression in AD brain cells, their association with senile plaques may not specifically indicate cadherin mRNA expression.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"19 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140156814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Verification of CRISPR/Cas9 Activity In Vitro via SSA-Based Dual-Luciferase Reporter System 通过基于 SSA 的双荧光素酶报告系统验证 CRISPR/Cas9 的体外活性

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700092

{"title":"Verification of CRISPR/Cas9 Activity In Vitro via SSA-Based Dual-Luciferase Reporter System","authors":"","doi":"10.1134/s0026893324700092","DOIUrl":"https://doi.org/10.1134/s0026893324700092","url":null,"abstract":"<span> <h3>Abstract</h3> <p>The CRISPR/Cas9 technique has emerged as a powerful and promising tool for precise genomic integration, which applied to various cell types and organisms, but its efficiency largely depends on single-guide RNA (sgRNA). There are multiple strategies available to evaluate the cleavage activity of sgRNAs, and one such approach is T7 endonuclease I (T7EI) assay, which is laborious and time consuming, especially when one must address multiple samples in parallel. In this study, a simple and rapid method to detect the cleavage activity of sgRNA was developed. Based on the single-strand annealing (SSA) repair mechanism, a surrogate reporter system using firefly luciferase was constructed to evaluate the targeting efficiency of sgRNAs. Using this system, the luciferase activities of eight sgRNAs were observed, and one of them had highest cutting efficiency (<em>p</em> < 0.01). Thereby, T7EI assay was compared with the method established in this study to determine the accuracy and sensitivity, and the results of these two methods were consistent suggesting that the SSA reporter system was compatible with T7EI assay. Compared with T7EI assay requiring multiple steps, such as PCR amplification, the SSA reporter system with one-step transfection can be completed on a large scale of sgRNAs within approximate two days. These findings suggested that SSA-based reporter system can accurately and rapidly evaluate the cleavage activities of multiple sgRNAs, thereby providing a robust and reliable process for CRISPR/Cas9 to select sgRNAs efficiently in genome editing.</p> </span>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140154249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promoter Mutation of the bZIP Transcription Factor BdABF Accelerates Flowering in Brachypodium distachyon bZIP 转录因子 BdABF 的启动子突变可加速大戟科植物开花

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700055

B. J. Han, Z. J. Liu, W. M. Bai, F. T. Sun, Y. F. Niu, T. Y. Song, W. J. You, P. Y. Wang, N. Li, P. S. Su

{"title":"Promoter Mutation of the bZIP Transcription Factor BdABF Accelerates Flowering in Brachypodium distachyon","authors":"B. J. Han, Z. J. Liu, W. M. Bai, F. T. Sun, Y. F. Niu, T. Y. Song, W. J. You, P. Y. Wang, N. Li, P. S. Su","doi":"10.1134/s0026893324700055","DOIUrl":"https://doi.org/10.1134/s0026893324700055","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Flowering time plays an important role in crop regional adaptation, yield and reproductive success. ABRE BINDING FACTORs (ABFs) are bZIP transcription factors that participate in various plant biological processes. However, only a few ABFs have been reported to function in controlling flowering time. Here, we identified and characterized the function of the <i>Brachypodium</i> bZIP transcription factor <i>BdABF</i> in controlling flowering time. The results showed that <i>Bdabf</i> mutation in the promoter region promoted early flowering time in <i>Bdabf</i> T-DNA mutants compared with wild-type (WT) plants. Transcriptomic analysis showed that, compared with WT plants, 447 differentially expressed genes (DEGs) (266 upregulated and 181 downregulated) were identified in circadian rhythm, ABA signaling, IAA signaling, and flavonoid biosynthesis pathways in <i>Bdabf</i> mutants. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) results showed that the expression profiles of these genes in various pathways were consistent with the RNA-seq-based transcriptome dataset. The TF binding element analysis of these genes involved in different pathways in the promoter region showed that bHLH, bZIP, NF-Y, TIFY, and WRKY transcription factors were enriched. Taken together, our results showed that <i>BdABF</i> was involved in controlling flowering time by activating various metabolic pathways and provided new insights into ABFs controlling flowering time in <i>Brachypodium</i>.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"15 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140156955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro 雷帕霉素调节脂多糖诱导的体外小胶质细胞吞噬作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700109

{"title":"Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro","authors":"","doi":"10.1134/s0026893324700109","DOIUrl":"https://doi.org/10.1134/s0026893324700109","url":null,"abstract":"<span> <h3>Abstract</h3> <p>Microglia phagocytosis plays an important role in the pathogenesis of neurodegeneration. Defects or dysfunction of microglia phagocytosis were observed in neurodegenerative diseases, with different targets and associated receptors influencing the microglia response. Moreover, non-canonical LC3-associated (microtubule-associated protein 1 light chain 3) phagocytosis was extensively studied recently as a novel form of phagocytosis on macrophages, but little on microglia. Here, we investigated changes in phagocytic function of microglia activated by lipopolysaccharide (LPS) as well as rapamycin-regulated phagocytosis. Phagocytosis in mouse BV2 cells and primary microglia was analyzed by flow cytometry and immunofluorescence. Phagocytosis-related mechanisms in BV2 cells were analyzed using Western blotting and real-time polymerase chain reaction. Rapamycin was shown to reduce LPS-induced phagocytosis of microglia and at the same time stimulate LС3-dependent phagocytosis by regulating <em>Atg3</em>, <em>Atg4</em> and <em>Atg7</em> expression. In addition, in BV2 cells, the PI3K/AKT/mTOR pathway may be involved in phagocytosis. These results suggest that phagocytosis of microglia is a complex process, and the increase in phagocytosis should not be considered as a maturation of phagocytic function. The data will provide new insights into the mechanisms of phagocytosis and neuroimmunity.</p> </span>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"36 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide Engineering Approach to Introduce an Improved Calcitonin Mutant 引入改良降钙素突变体的肽工程方法

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700110

M. Zarei, B. Abedini, A. Dehshahri, M. Negahdaripour

{"title":"Peptide Engineering Approach to Introduce an Improved Calcitonin Mutant","authors":"M. Zarei, B. Abedini, A. Dehshahri, M. Negahdaripour","doi":"10.1134/s0026893324700110","DOIUrl":"https://doi.org/10.1134/s0026893324700110","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Calcitonin is a 32-amino acid peptide, which causes a decrease in blood calcium levels and bone resorption in the human body. It is produced in different species. Salmon calcitonin is used as a medicine for diseases such as osteoporosis, Paget’s disease, and hypercalcemia. However, the salmon calcitonin peptide used as a medicine, could induce immune responses in humans. Decreasing the antigenicity of salmon calcitonin could improve this molecule for pharmaceutical usage. In this study, improving physicochemical properties and reducing allergenicity and especially the antigenicity of salmon calcitonin were followed. The calcitonin sequences of different species were evaluated, and those with better properties were considered as a guide for peptide engineering. In silico methods were utilized to characterize the properties of the reviewed calcitonin sequences, and the best sequences (the calcitonin of sheep, dog, rat, and human) were used as a template to decrease the antigenicity of salmon calcitonin. The epitopic parts, <i>i.e.,</i> amino acids 16 to 29, were identified by different servers. Hot spot residues including Y22, N26, T27, and S29 were characterized based on the main criteria of being a B-cell epitope including convexity index, hydrophilicity, and surface accessibility. These residues were replaced with lower antigenic counterparts. Results showed the final selected mutant (T27V/S29V) had a lower antigenicity and higher solubility and stability than salmon calcitonin. Thus, our suggested mutant could be a potential alternative candidate to salmon calcitonin. However, future in vitro and in vivo evaluations are needed to confirm its suitability for clinical usage.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"98 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19 枯草芽孢杆菌细胞外膜囊泡的蛋白质组 3-19

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010059

W. Kurdy, P. V. Zelenikhin, G. Yu. Yakovleva, M. N. Sinyagina, A. I. Kolpakov, O. N. Ilinskaya

{"title":"The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19","authors":"W. Kurdy, P. V. Zelenikhin, G. Yu. Yakovleva, M. N. Sinyagina, A. I. Kolpakov, O. N. Ilinskaya","doi":"10.1134/s0026893324010059","DOIUrl":"https://doi.org/10.1134/s0026893324010059","url":null,"abstract":"<p><b>Abstract</b>—Production of extracellular membrane vesicles plays an important role in communication in bacterial populations and in bacteria–host interactions. Vesicles as carriers of various regulatory and signaling molecules may be potentially used as disease biomarkers and promising therapeutic agents, including vaccine preparations. The composition of membrane vesicles has been deciphered for a limited number of Gram-negative and Gram-positive bacteria. In this work, for the first time, extracellular membrane vesicles of a streptomycin-resistant strain <i>Bacillus pumilus</i> 3-19, a producer of extracellular guanyl-preferring ribonuclease binase, are isolated, visualized, and characterized by their genome and proteome composition. It has been established that there is no genetic material in the vesicles and the spectrum of the proteins differs depending on the phosphate content in the culture medium of the strain. Vesicles from a phosphate-deficient medium carry 49 unique proteins in comparison with 101 from a medium with the high phosphate content. The two types of vesicles had 140 mutual proteins. Flagellar proteins, RNase J, which is the main enzyme of RNA degradosomes, phosphatases, peptidases, iron transporters, signal peptides, were identified in vesicles. Antibiotic resistance proteins and amyloid-like proteins whose genes are present in <i>B. pumilus</i> 3-19 cells are absent. Phosphate deficiency-induced binase was found only in vesicles from a phosphate-deficient medium.</p>","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"34 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0