Molecular Biology最新文献_第6页

Promoter Mutation of the bZIP Transcription Factor BdABF Accelerates Flowering in Brachypodium distachyon bZIP 转录因子 BdABF 的启动子突变可加速大戟科植物开花

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700055

B. J. Han, Z. J. Liu, W. M. Bai, F. T. Sun, Y. F. Niu, T. Y. Song, W. J. You, P. Y. Wang, N. Li, P. S. Su

{"title":"Promoter Mutation of the bZIP Transcription Factor BdABF Accelerates Flowering in Brachypodium distachyon","authors":"B. J. Han, Z. J. Liu, W. M. Bai, F. T. Sun, Y. F. Niu, T. Y. Song, W. J. You, P. Y. Wang, N. Li, P. S. Su","doi":"10.1134/s0026893324700055","DOIUrl":"https://doi.org/10.1134/s0026893324700055","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Flowering time plays an important role in crop regional adaptation, yield and reproductive success. ABRE BINDING FACTORs (ABFs) are bZIP transcription factors that participate in various plant biological processes. However, only a few ABFs have been reported to function in controlling flowering time. Here, we identified and characterized the function of the Brachypodium bZIP transcription factor BdABF in controlling flowering time. The results showed that Bdabf mutation in the promoter region promoted early flowering time in Bdabf T-DNA mutants compared with wild-type (WT) plants. Transcriptomic analysis showed that, compared with WT plants, 447 differentially expressed genes (DEGs) (266 upregulated and 181 downregulated) were identified in circadian rhythm, ABA signaling, IAA signaling, and flavonoid biosynthesis pathways in Bdabf mutants. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) results showed that the expression profiles of these genes in various pathways were consistent with the RNA-seq-based transcriptome dataset. The TF binding element analysis of these genes involved in different pathways in the promoter region showed that bHLH, bZIP, NF-Y, TIFY, and WRKY transcription factors were enriched. Taken together, our results showed that BdABF was involved in controlling flowering time by activating various metabolic pathways and provided new insights into ABFs controlling flowering time in Brachypodium.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"15 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140156955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro 雷帕霉素调节脂多糖诱导的体外小胶质细胞吞噬作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700109

{"title":"Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro","authors":"","doi":"10.1134/s0026893324700109","DOIUrl":"https://doi.org/10.1134/s0026893324700109","url":null,"abstract":" <h3>Abstract</h3> Microglia phagocytosis plays an important role in the pathogenesis of neurodegeneration. Defects or dysfunction of microglia phagocytosis were observed in neurodegenerative diseases, with different targets and associated receptors influencing the microglia response. Moreover, non-canonical LC3-associated (microtubule-associated protein 1 light chain 3) phagocytosis was extensively studied recently as a novel form of phagocytosis on macrophages, but little on microglia. Here, we investigated changes in phagocytic function of microglia activated by lipopolysaccharide (LPS) as well as rapamycin-regulated phagocytosis. Phagocytosis in mouse BV2 cells and primary microglia was analyzed by flow cytometry and immunofluorescence. Phagocytosis-related mechanisms in BV2 cells were analyzed using Western blotting and real-time polymerase chain reaction. Rapamycin was shown to reduce LPS-induced phagocytosis of microglia and at the same time stimulate LС3-dependent phagocytosis by regulating Atg3, Atg4 and Atg7 expression. In addition, in BV2 cells, the PI3K/AKT/mTOR pathway may be involved in phagocytosis. These results suggest that phagocytosis of microglia is a complex process, and the increase in phagocytosis should not be considered as a maturation of phagocytic function. The data will provide new insights into the mechanisms of phagocytosis and neuroimmunity. ","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"36 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide Engineering Approach to Introduce an Improved Calcitonin Mutant 引入改良降钙素突变体的肽工程方法

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-17 DOI: 10.1134/s0026893324700110

M. Zarei, B. Abedini, A. Dehshahri, M. Negahdaripour

{"title":"Peptide Engineering Approach to Introduce an Improved Calcitonin Mutant","authors":"M. Zarei, B. Abedini, A. Dehshahri, M. Negahdaripour","doi":"10.1134/s0026893324700110","DOIUrl":"https://doi.org/10.1134/s0026893324700110","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Calcitonin is a 32-amino acid peptide, which causes a decrease in blood calcium levels and bone resorption in the human body. It is produced in different species. Salmon calcitonin is used as a medicine for diseases such as osteoporosis, Paget’s disease, and hypercalcemia. However, the salmon calcitonin peptide used as a medicine, could induce immune responses in humans. Decreasing the antigenicity of salmon calcitonin could improve this molecule for pharmaceutical usage. In this study, improving physicochemical properties and reducing allergenicity and especially the antigenicity of salmon calcitonin were followed. The calcitonin sequences of different species were evaluated, and those with better properties were considered as a guide for peptide engineering. In silico methods were utilized to characterize the properties of the reviewed calcitonin sequences, and the best sequences (the calcitonin of sheep, dog, rat, and human) were used as a template to decrease the antigenicity of salmon calcitonin. The epitopic parts, i.e., amino acids 16 to 29, were identified by different servers. Hot spot residues including Y22, N26, T27, and S29 were characterized based on the main criteria of being a B-cell epitope including convexity index, hydrophilicity, and surface accessibility. These residues were replaced with lower antigenic counterparts. Results showed the final selected mutant (T27V/S29V) had a lower antigenicity and higher solubility and stability than salmon calcitonin. Thus, our suggested mutant could be a potential alternative candidate to salmon calcitonin. However, future in vitro and in vivo evaluations are needed to confirm its suitability for clinical usage.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"98 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19 枯草芽孢杆菌细胞外膜囊泡的蛋白质组 3-19

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010059

W. Kurdy, P. V. Zelenikhin, G. Yu. Yakovleva, M. N. Sinyagina, A. I. Kolpakov, O. N. Ilinskaya

{"title":"The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19","authors":"W. Kurdy, P. V. Zelenikhin, G. Yu. Yakovleva, M. N. Sinyagina, A. I. Kolpakov, O. N. Ilinskaya","doi":"10.1134/s0026893324010059","DOIUrl":"https://doi.org/10.1134/s0026893324010059","url":null,"abstract":"Abstract—Production of extracellular membrane vesicles plays an important role in communication in bacterial populations and in bacteria–host interactions. Vesicles as carriers of various regulatory and signaling molecules may be potentially used as disease biomarkers and promising therapeutic agents, including vaccine preparations. The composition of membrane vesicles has been deciphered for a limited number of Gram-negative and Gram-positive bacteria. In this work, for the first time, extracellular membrane vesicles of a streptomycin-resistant strain Bacillus pumilus 3-19, a producer of extracellular guanyl-preferring ribonuclease binase, are isolated, visualized, and characterized by their genome and proteome composition. It has been established that there is no genetic material in the vesicles and the spectrum of the proteins differs depending on the phosphate content in the culture medium of the strain. Vesicles from a phosphate-deficient medium carry 49 unique proteins in comparison with 101 from a medium with the high phosphate content. The two types of vesicles had 140 mutual proteins. Flagellar proteins, RNase J, which is the main enzyme of RNA degradosomes, phosphatases, peptidases, iron transporters, signal peptides, were identified in vesicles. Antibiotic resistance proteins and amyloid-like proteins whose genes are present in B. pumilus 3-19 cells are absent. Phosphate deficiency-induced binase was found only in vesicles from a phosphate-deficient medium.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"34 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prime-Editing Methods and pegRNA Design Programs 基质编辑方法和 pegRNA 设计程序

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010084

E. V. Mikhaylova, B. R. Kuluev, G. A. Gerashchenkov, D. A. Chemeris, R. R. Garafutdinov, A. R. Kuluev, An. K. Baymiev, Al. K. Baymiev, A. V. Chemeris

{"title":"Prime-Editing Methods and pegRNA Design Programs","authors":"E. V. Mikhaylova, B. R. Kuluev, G. A. Gerashchenkov, D. A. Chemeris, R. R. Garafutdinov, A. R. Kuluev, An. K. Baymiev, Al. K. Baymiev, A. V. Chemeris","doi":"10.1134/s0026893324010084","DOIUrl":"https://doi.org/10.1134/s0026893324010084","url":null,"abstract":"Abstract—It has been 10 years since CRISPR/Cas technology was applied to edit the genomes of various organisms. Its ability to produce a double-strand break in a DNA region specified by the researcher started a revolution in bioengineering. Later, the Base Editing (BE) method was developed. BE is performed via the formation of single-strand breaks by the mutant form of Cas nuclease (nickase), fused with deaminases and other enzymes. It can be used to promote A ( leftrightarrow ) G and C ( leftrightarrow ) T transitions, and a C → G transversion. Just over 3 years ago, a new Prime Editing (PE) variant of CRISPR/Cas was invented. Unlike BE, in PE the nickase is fused with reverse transcriptase, capable of building a new DNA chain using the pegRNA template. The pegRNA consists of an elongated guide RNA with an extra sequence at the 3'-end. Prime editing makes it possible to insert the desired mutations into this extra sequence and to carry out any substitutions and indels of bases without the use of special donor DNA. To date, a number of PE variants have been proposed; they are briefly considered in this review with an emphasis on prime editing of plant genomes. Some attention is also paid to pegRNA design programs, as well as evaluation of the efficiency of the editing. Such a variety of PE techniques is due to the opportunities of high-precision introduction of desired changes with a rather low frequency of off-target mutations in the genomes of various organisms. The relatively low efficiency of prime editing inspires researchers to offer new approaches. There is hope that further development of the technology will improve PE enough to take its rightful place among the genome targeting methods that are suitable for any organisms, and will have a positive impact on the agricultural sector, industrial biotechnologies, and medicine.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"278 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Retrotransposons in Drosophila melanogaster Somatic Tissues 黑腹果蝇体细胞组织中逆转录转座子的调控

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010096

P. A. Milyaeva, I. V. Kukushkina, A. R. Lavrenov, I. V. Kuzmin, A. I. Kim, L. N. Nefedova

引用次数: 0

Structure and Evolution of DNA Transposons of the L31 Superfamily in Bivalves 双壳类动物中 L31 超家族 DNA 转座子的结构与进化

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010114

M. V. Puzakov, L. V. Puzakova

{"title":"Structure and Evolution of DNA Transposons of the L31 Superfamily in Bivalves","authors":"M. V. Puzakov, L. V. Puzakova","doi":"10.1134/s0026893324010114","DOIUrl":"https://doi.org/10.1134/s0026893324010114","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The mobile genetic elements IS630/Tc1/mariner (ITm) are widespread DNA transposons that make a significant contribution to the evolution of eukaryotic genomes. With the start of large-scale application of next-generation sequencing (NGS) technologies and the emergence of many new whole genome sequences of organisms in nucleotide sequence collections, the ITm elements have been identified in most taxa of the eukaryotic tree of life. Although ITm diversity has been studied in detail, new elements are still found, thus expanding the respective DNA transposon group and calling for review of its classification. Bivalve L31 elements were for the first time analyzed in detail to describe their structures, diversity, distribution, and phylogenetic position among the ITm elements. The L31 transposons were found to form an independent superfamily of an ancient origin within the ITm group. Rather high diversity was observed within the L31 clade; i.e., five phylogenetic clusters were identified. In mollusks, the L31 transposons have been detected only in the subclass Autobranchia and predominate in diversity and number in the infraclass Pteriomorphia. A protein encoded by open reading frame 2 (ORF2) was shown to be an integral structural component of almost all full-length L31 elements. The results provide for a better understanding of the evolution of particular ITm transposons. Further study of the L31 transposons in other taxa (cnidarians) and functional investigation of the ORF2 protein product will help to better understand the evolution of DNA transposons, the mechanisms of their horizontal transfer, and their contribution to eukaryotic biodiversity.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"106 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Group of New Hypermethylated Long Non-Coding RNA Genes Associated with the Development and Progression of Breast Cancer 一组与乳腺癌的发生和发展有关的高甲基化长非编码 RNA 新基因

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010035

E. A. Filippova, V. I. Loginov, S. S. Lukina, A. M. Burdennyy, I. V. Pronina, T. P. Kazubskaya, E. A. Braga

{"title":"A Group of New Hypermethylated Long Non-Coding RNA Genes Associated with the Development and Progression of Breast Cancer","authors":"E. A. Filippova, V. I. Loginov, S. S. Lukina, A. M. Burdennyy, I. V. Pronina, T. P. Kazubskaya, E. A. Braga","doi":"10.1134/s0026893324010035","DOIUrl":"https://doi.org/10.1134/s0026893324010035","url":null,"abstract":"Abstract—Breast cancer is the most common type of cancer among women. The study of the mechanisms of metastasis, the main cause of death from breast cancer, as well as the search for new markers for early diagnosis and prognosis of breast cancer, is an extremely topical issue. New perspectives in the diagnosis and treatment of breast cancer are opened by the mechanisms of gene regulation involving non-coding RNAs, in particular, long non-coding RNAs (lncRNAs). In this work, we analyzed the methylation levels of seven lncRNA genes (MEG3, SEMA3B-AS1, HAND2-AS1, KCNK15-AS1, ZNF667-AS1, MAGI2-AS3, and PLUT) by quantitative methyl-specific PCR on a set of 79 paired (tumor/normal) samples of breast cancer. Hypermethylation of all seven lncRNA genes was revealed, and hypermethylation of HAND2-AS1, KCNK15-AS1, MAGI2-AS3, and PLUT was detected in breast cancer for the first time. It was found that the level of methylation of the studied lncRNA genes correlated statistically significantly with the stage of the tumor process, the size of the tumor, and the presence of metastases in the lymph nodes. Thus, methylation of the seven studied lncRNA genes is associated with the development and progression of breast cancer, and these genes can be useful as potential markers in the diagnosis and prognosis of breast cancer.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"133 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationships between Cxcl12, Tweak, Notch1, and Yap mRNA Expression Levels in Molecular Mechanisms of Liver Fibrogenesis 肝纤维化分子机制中 Cxcl12、Tweak、Notch1 和 Yap mRNA 表达水平之间的关系

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010060

E. I. Lebedeva, A. T. Shchastniy, A. S. Babenka, D. A. Zinovkin

{"title":"Relationships between Cxcl12, Tweak, Notch1, and Yap mRNA Expression Levels in Molecular Mechanisms of Liver Fibrogenesis","authors":"E. I. Lebedeva, A. T. Shchastniy, A. S. Babenka, D. A. Zinovkin","doi":"10.1134/s0026893324010060","DOIUrl":"https://doi.org/10.1134/s0026893324010060","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Current data on the molecular mechanisms of liver fibrosis and cirrhosis fail to fully explain all stages of their development. Interactions between individual genes and signaling pathways are known to play an important role in their functions. However, data on their relationships are insufficient and often contradictory. For the first time, mRNA expression of Notch1, Notch2, Yap1, Tweak (Tnfsf12), Fn14 (Tnfrsf12a), Ang, Vegfa, Cxcl12 (Sdf), Nos2, and Mmp-9 was studied in detail at several stages of thioacetamide-induced liver fibrosis in Wistar rats. A factor analysis isolated three factors, which combined highly correlated target genes. The first factor included four genes: Cxcl12 (r = 0.829, p < 0.05), Tweak (r = 0.841, p < 0.05), Notch1 (r = 0.848, p < 0.05), and Yap1 (r = 0.921, p < 0.05). The second factor described the correlation between Mmp-9 (r = 0.791, p < 0.05) and Notch2 (r = 0.836, p < 0.05). The third factor included Ang (r = 0.748, p < 0.05) and Vegfa (r = 0.679, p < 0.05). The Nos2 and Fn14 genes were not included in any of the factors. The gene grouping by mRNA expression levels made it possible to assume a pathogenetic relationship between their products in the development of fibrotic changes due to liver toxicity.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"16 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences 各种波长的紫外线辐射造成细胞 DNA 损伤的光化学过程：生物学后果

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010047

G. Ya. Fraikin, N. S. Belenikina, A. B. Rubin

{"title":"Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences","authors":"G. Ya. Fraikin, N. S. Belenikina, A. B. Rubin","doi":"10.1134/s0026893324010047","DOIUrl":"https://doi.org/10.1134/s0026893324010047","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Photochemical reactions in cell DNA are induced in various organisms by solar UV radiation and may lead to a series of biological responses to DNA damage, including apoptosis, mutagenesis, and carcinogenesis. The chemical nature and the amount of DNA lesions depend on the wavelength of UV radiation. UV type B (UVB, 290–320 nm) causes two main lesions, cyclobutane pyrimidine dimers (CPDs) and, with a lower yield, pyrimidine (6-4) pyrimidone photoproducts (6-4PPs). Their formation is a result of direct UVB photon absorption by DNA bases. UV type A (UVA, 320–400 nm) induces only cyclobutane dimers, which most likely arise via triplet–triplet energy transfer (TTET) from cell chromophores to DNA thymine bases. UVA is much more effective than UVB in inducing sensitized oxidative DNA lesions, such as single-strand breaks and oxidized bases. Of the latter, 8-oxo-dihydroguanine (8-oxodG) is the most frequent, being produced in several oxidation processes. Many recent studies reported novel, more detailed information about the molecular mechanisms of the photochemical reactions that underlie the formation of various DNA lesions. The information is mostly summarized and analyzed in the review. Special attention is paid to the oxidation reactions that are initiated by reactive oxygen species (ROS) and radicals generated by potential endogenous photosensitizers, such as pterins, riboflavin, protoporphyrin IX, NADH, and melanin. The review discusses the role that specific DNA photoproducts play in genotoxic processes induced in living systems by UV radiation of various wavelengths, including human skin carcinogenesis.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"5 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0