Promoter Mutation of the bZIP Transcription Factor BdABF Accelerates Flowering in Brachypodium distachyon

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Pub Date : 2024-03-17 DOI:10.1134/s0026893324700055

B. J. Han, Z. J. Liu, W. M. Bai, F. T. Sun, Y. F. Niu, T. Y. Song, W. J. You, P. Y. Wang, N. Li, P. S. Su

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Abstract

Flowering time plays an important role in crop regional adaptation, yield and reproductive success. ABRE BINDING FACTORs (ABFs) are bZIP transcription factors that participate in various plant biological processes. However, only a few ABFs have been reported to function in controlling flowering time. Here, we identified and characterized the function of the Brachypodium bZIP transcription factor BdABF in controlling flowering time. The results showed that Bdabf mutation in the promoter region promoted early flowering time in Bdabf T-DNA mutants compared with wild-type (WT) plants. Transcriptomic analysis showed that, compared with WT plants, 447 differentially expressed genes (DEGs) (266 upregulated and 181 downregulated) were identified in circadian rhythm, ABA signaling, IAA signaling, and flavonoid biosynthesis pathways in Bdabf mutants. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) results showed that the expression profiles of these genes in various pathways were consistent with the RNA-seq-based transcriptome dataset. The TF binding element analysis of these genes involved in different pathways in the promoter region showed that bHLH, bZIP, NF-Y, TIFY, and WRKY transcription factors were enriched. Taken together, our results showed that BdABF was involved in controlling flowering time by activating various metabolic pathways and provided new insights into ABFs controlling flowering time in Brachypodium.

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bZIP 转录因子 BdABF 的启动子突变可加速大戟科植物开花

摘要开花时间对作物的区域适应性、产量和繁殖成功率起着重要作用。ABRE BINDING FACTORs（ABFs）是一种 bZIP 转录因子，参与多种植物生物过程。然而，只有少数 ABFs 被报道具有控制花期的功能。在此，我们鉴定并描述了蕨类植物 bZIP 转录因子 BdABF 在控制花期方面的功能。结果表明，与野生型（WT）植株相比，Bdabf T-DNA 突变体启动子区的 Bdabf 突变会促进 Bdabf T-DNA 突变体提前开花。转录组分析表明，与WT植株相比，Bdabf突变体在昼夜节律、ABA信号转导、IAA信号转导和类黄酮生物合成途径中发现了447个差异表达基因（DEGs）（266个上调，181个下调）。定量反转录聚合酶链反应（qRT-PCR）结果表明，这些基因在不同通路中的表达谱与基于RNA-seq的转录组数据集一致。对这些参与不同途径的基因启动子区域的转录因子结合元件分析表明，bHLH、bZIP、NF-Y、TIFY 和 WRKY 转录因子被富集。综上所述，我们的研究结果表明，BdABF 通过激活各种代谢途径参与了花期的控制，并为研究控制臂柄植物花期的 ABFs 提供了新的视角。

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来源期刊

Molecular Biology 生物-生化与分子生物学

CiteScore

1.30

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.