Molecular Biology最新文献_第10页

Metabolic Stress of Red Blood Cells Induces Hemoglobin Glutathionylation 红细胞的代谢压力诱导血红蛋白的谷胱甘肽化

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060225

P. I. Zaripov, Yu. D. Kuleshova, Yu. M. Poluektov, S. V. Sidorenko, O. K. Kvan, G. V. Maksimov, V. A. Mitkevich, A. A. Makarov, I. Yu. Petrushanko

{"title":"Metabolic Stress of Red Blood Cells Induces Hemoglobin Glutathionylation","authors":"P. I. Zaripov, Yu. D. Kuleshova, Yu. M. Poluektov, S. V. Sidorenko, O. K. Kvan, G. V. Maksimov, V. A. Mitkevich, A. A. Makarov, I. Yu. Petrushanko","doi":"10.1134/s0026893323060225","DOIUrl":"https://doi.org/10.1134/s0026893323060225","url":null,"abstract":"Abstract—Metabolic stress caused by a lack of glucose significantly affects the state of red blood cells, where glycolysis is the main pathway for the production of ATP. Hypoglycemia can be both physiological (occurring during fasting and heavy physical exertion) and pathological (accompanying a number of diseases, such as diabetes mellitus). In this study, we have characterized the state of isolated erythrocytes under metabolic stress caused by the absence of glucose. It was established that 24 h of incubation of the erythrocytes in a glucose-free medium to simulate blood plasma led to a two-fold decrease in the ATP level into them. The cell size, as well as intracellular sodium concentration increased. These findings could be the result of a disruption in ion transporter functioning because of a decrease in the ATP level. The calcium level remained unchanged. With a lack of glucose in the medium of isolated erythrocytes, there was no increase in ROS and a significant change in the level of nitric oxide, while the level of the main low-molecular weight thiol of cells, glutathione (GSH) decreased by almost 2 times. It was found that the metabolic stress of isolated red blood cells induced hemoglobin glutathionylation despite the absence of ROS growth. The cause was the lack of ATP, which led to a decrease in the level of GSH because of the inhibition of its synthesis and, probably, due to a decrease in the NADPH level required for glutathione (GSSG) reduction and protein deglutathionylation. Thus, erythrocyte metabolic stress induced hemoglobin glutathionylation, which is not associated with an increase in ROS. This may have an important physiological significance, since glutathionylation of hemoglobin changes its affinity for oxygen.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"3 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138555983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Metabolism and the Role of Redox Factors in the Energy Control of Quiescence and Proliferation of Hematopoietic Cells 代谢调节和氧化还原因子在造血细胞衰老和增殖的能量控制中的作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060080

M. V. Kalashnikova, N. S. Polyakova, A. V. Belyavsky

{"title":"Regulation of Metabolism and the Role of Redox Factors in the Energy Control of Quiescence and Proliferation of Hematopoietic Cells","authors":"M. V. Kalashnikova, N. S. Polyakova, A. V. Belyavsky","doi":"10.1134/s0026893323060080","DOIUrl":"https://doi.org/10.1134/s0026893323060080","url":null,"abstract":"Abstract—One of the key regulators of hematopoietic stem cell (HSC) maintenance is cellular metabolism. Resting HSCs use anaerobic glycolysis as the main source of energy. During expansion and differentiation under conditions of steady state hematopoiesis, the energy needs of activated HSCs increase by many fold. To meet the increased demands, cells switch to mitochondrial oxidative phosphorylation, which is accompanied by an increase in reactive oxygen species (ROS) production. Here, the molecular mechanisms maintaining glycolysis in HSCs, as well as the factors determining the increase in metabolic activity and the transition to mitochondrial biogenesis during HSC activation are discussed. We focus on the role of HIF (hypoxia-inducible factor) proteins as key mediators of the cellular response to hypoxia, and also consider the phenomenon of extraphysiological oxygen shock (EPHOSS), leading to the forced differentiation of HSCs as well as methods of overcoming it. Finally, the role of fatty acid oxidation (FAO) in hematopoiesis is discussed. Understanding the metabolic needs of normal HSCs and precursors is crucial for the development of new treatments for diseases related to the hematopoietic and immune systems.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"30 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric Oxide(II) in the Biology of Chlorophyta 叶绿体生物学中的一氧化氮（II）

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060055

E. V. Ermilova

{"title":"Nitric Oxide(II) in the Biology of Chlorophyta","authors":"E. V. Ermilova","doi":"10.1134/s0026893323060055","DOIUrl":"https://doi.org/10.1134/s0026893323060055","url":null,"abstract":"Abstract—NO is a gaseous signaling redox-active molecule that functions in various eukaryotes. However, its synthesis, turnover, and effects in cells are specific in plants in several aspects. Compared with higher plants, the role of NO in Chlorophyta has not been investigated enough. However, some of the mechanisms for controlling the levels of this signaling molecule have been characterized in model green algae. In Chlamydomonas reinhardtii, NO synthesis is carried out by a dual system of nitrate reductase and NO-forming nitrite reductase. Other mechanisms that might produce NO from nitrite are associated with components of the mitochondrial electron-transport chain. In addition, NO formation in some green algae proceeds by an oxidative mechanism similar to that in mammals. The recent discovery of L-arginine-dependent NO synthesis in the colorless alga Polytomella parva suggests the existence of a protein complex with enzyme activities that are similar to animal nitric oxide synthase. This latter finding paves the way for further research into potential members of the NO synthases family in Chlorophyta. Beyond synthesis, the regulatory processes to maintain intracellular NO levels are also an integral part for its function in cells. Members of the truncated hemoglobins family with dioxygenase activity can convert NO to nitrate, as was shown for C. reinhardtii. In addition, the implication of NO reductases in NO scavenging has also been described. Even more intriguing, unlike in animals, the typical NO/cGMP signaling module appears not to be used by green algae. S-nitrosylated glutathione, which is considered the main reservoir for NO, provides NO signals to proteins. In Chlorophyta, protein S-nitrosation is one of the key mechanisms of action of the redox molecule. In this review, we discuss the current state-of-the-art and possible future directions related to the biology of NO in green algae.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"55 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Redox-Catalytic Properties of Cobalamins 钴胺的氧化还原催化特性

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060158

Yu. V. Shatalin, V. S. Shubina, M. E. Solovieva, V. S. Akatov

引用次数: 0

The Antioxidant and Geroprotective Properties of an Extract of Mountain Ash (Sorbus aucuparia L.) Fruits 山白蜡果实提取物的抗氧化和老年保护特性

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060134

E. Yu. Platonova, D. A. Golubev, N. V. Zemskaya, O. G. Shevchenko, S. A. Patov, M. V. Shaposhnikov, A. A. Moskalev

{"title":"The Antioxidant and Geroprotective Properties of an Extract of Mountain Ash (Sorbus aucuparia L.) Fruits","authors":"E. Yu. Platonova, D. A. Golubev, N. V. Zemskaya, O. G. Shevchenko, S. A. Patov, M. V. Shaposhnikov, A. A. Moskalev","doi":"10.1134/s0026893323060134","DOIUrl":"https://doi.org/10.1134/s0026893323060134","url":null,"abstract":"Abstract—Plant polyphenols are characterized by a wide range of biological activities, including antioxidant properties, and have a high geroprotective potential. The purpose of this work was to investigate the effect of the extract of rowan berries (Sorbus aucuparia L.) on the lifespan and stress resistance of Drosophila melanogaster with the identification of possible mechanisms of its biological activity. It has been established that an ethanol extract of S. aucuparia berries, the main components of which are rutin and cyanidin-3-rutinoside, has a pronounced antioxidant activity in vitro. At the same time, treatment with rowan berry extract increased the resistance of D. melanogaster males to starvation, but reduced resistance to hyperthermia. In females, the extract reduced resistance to oxidative stress but increased resistance to hyperthermia. The effects of rowan berry extract on longevity depended both on its concentration and on the sex of fruit flies. In response to treatment with rowan berry extract, D. melanogaster males and females showed slight differences in the background level of expression of cellular stress response genes, including heat shock genes (hsp27, hsp68, and hsp83), oxidative stress resistance genes (hif1, nrf2, and sod1), circadian rhythm genes (clk and per), and the longevity gene sirt1, which may explain the differences in the observed effects.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"49 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Melittin-Like Proteins with a Molecular Weight of 67 kDa that Interact with Na+/K+-ATPase 鉴定与 Na+/K+-ATP 酶相互作用的分子量为 67 kDa 的 Melittin 类蛋白

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060195

L. A. Varfolomeeva, E. A. Klimanova, S. V. Sidorenko, D. A. Fedorov, O. D. Lopina

{"title":"Identification of Melittin-Like Proteins with a Molecular Weight of 67 kDa that Interact with Na+/K+-ATPase","authors":"L. A. Varfolomeeva, E. A. Klimanova, S. V. Sidorenko, D. A. Fedorov, O. D. Lopina","doi":"10.1134/s0026893323060195","DOIUrl":"https://doi.org/10.1134/s0026893323060195","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Melittin, a peptide from bee venom, was found to be able to interact with many proteins, including calmodulin target proteins and ion-transporting P-type ATPases. It is assumed that melittin mimics a protein module involved in protein-protein interactions within cells. Previously, a Na+/K+-ATPase containing the α1 isoform of the catalytic subunit was found to co-precipitate with a protein with a molecular weight of about 70 kDa that interacts with antibodies against melittin by cross immunoprecipitation. In the presence of a specific Na+/K+-ATPase inhibitor (ouabain), the amount of protein with a molecular weight of 70 kDa interacting with Na+/K+-ATPase increases. In order to identify melittin-like protein from murine kidney homogenate, a fraction of melittin-like proteins with a molecular weight of approximately 70 kDa was obtained using affinity chromatography with immobilized antibodies specific to melittin. By mass spectrometry analysis, the obtained protein fraction was found to contain three molecular chaperones of Hsp70 superfamily: mitochondrial mtHsp70 (mortalin), Hsp73, Grp78 (BiP) of endoplasmic reticulum. These data suggest that chaperones from the HSP-70 superfamily contain a melittin-like module.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"6 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of δ-Aminolevulinic Acid Dehydratase of the Cold-Water Sponge Halisarca dujardinii 冷水海绵 Halisarca dujardinii 的 δ-Aminolevulinic Acid Dehydratase 的特征

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060109

O. I. Kravchuk, A. D. Finoshin, K. V. Mikhailov, R. H. Ziganshin, K. I. Adameyko, N. G. Gornostaev, A. I. Zhurakovskaya, V. S. Mikhailov, E. I. Shagimardanova, Yu. V. Lyupina

引用次数: 0

The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin 胸腺激素胸腺素-1α可降低内毒素诱导的生264.7细胞的促炎反应

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060110

E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk

{"title":"The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin","authors":"E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk","doi":"10.1134/s0026893323060110","DOIUrl":"https://doi.org/10.1134/s0026893323060110","url":null,"abstract":"Abstract—The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the Tlr4 gene. Third, Tα1 significantly reduced p53 and the activity of the P53 gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in Ar-1 gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"68 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Mitochondria in Intestinal Epithelial Barrier Dysfunction in Inflammatory Bowel Disease 线粒体在炎症性肠病肠道上皮屏障功能障碍中的作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S0026893323060043

D. A. Chernyavskij, I. I. Galkin, A. N. Pavlyuchenkova, A. Fedorov, M. Chelombitko

引用次数: 0

Neuronal Calcium Sensor-1: A Zinc/Redox-Dependent Protein of Nervous System Signaling Pathways 神经元钙传感器-1：神经系统信号通路的锌/氧化还原依赖蛋白

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S002689332306002X

V. E. Baksheeva, Jr A. A. Zamyatnin, E. Y. Zernii

引用次数: 0