Molecular Biology最新文献_第9页

Characteristics of δ-Aminolevulinic Acid Dehydratase of the Cold-Water Sponge Halisarca dujardinii 冷水海绵 Halisarca dujardinii 的 δ-Aminolevulinic Acid Dehydratase 的特征

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060109

O. I. Kravchuk, A. D. Finoshin, K. V. Mikhailov, R. H. Ziganshin, K. I. Adameyko, N. G. Gornostaev, A. I. Zhurakovskaya, V. S. Mikhailov, E. I. Shagimardanova, Yu. V. Lyupina

引用次数: 0

The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin 胸腺激素胸腺素-1α可降低内毒素诱导的生264.7细胞的促炎反应

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-07 DOI: 10.1134/s0026893323060110

E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk

{"title":"The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin","authors":"E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk","doi":"10.1134/s0026893323060110","DOIUrl":"https://doi.org/10.1134/s0026893323060110","url":null,"abstract":"Abstract—The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the Tlr4 gene. Third, Tα1 significantly reduced p53 and the activity of the P53 gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in Ar-1 gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"68 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Mitochondria in Intestinal Epithelial Barrier Dysfunction in Inflammatory Bowel Disease 线粒体在炎症性肠病肠道上皮屏障功能障碍中的作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S0026893323060043

D. A. Chernyavskij, I. I. Galkin, A. N. Pavlyuchenkova, A. Fedorov, M. Chelombitko

引用次数: 0

Neuronal Calcium Sensor-1: A Zinc/Redox-Dependent Protein of Nervous System Signaling Pathways 神经元钙传感器-1：神经系统信号通路的锌/氧化还原依赖蛋白

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S002689332306002X

V. E. Baksheeva, Jr A. A. Zamyatnin, E. Y. Zernii

引用次数: 0

Low-Molecular Thiols as a Factor Improving the Sensitivity of Escherichia coli Mutants with Impaired ADP–Heptose Synthesis to Antibiotics 低分子硫醇是提高 ADP-缩酮合成受损的大肠杆菌突变体对抗生素敏感性的一个因素

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S0026893323060146

T. A. Seregina, I. Petrushanko, P. I. Zaripov, Yu. D. Kuleshova, K. V. Lobanov, R. Shakulov, V. Mitkevich, A. Makarov, A. Mironov

引用次数: 0

Transcription Factor NRF2 in Endothelial Functions 内皮功能中的转录因子 NRF2

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S0026893323060092

N. D. Kondratenko, L. A. Zinovkina, R. Zinovkin

引用次数: 0

Dinityrosyl Iron Complexes with Thiol-Containing Ligands as a Functionally Active “Working Form” of Nitric Oxide System in Living Organisms: A Review 含硫醇配体的二蒽络合铁作为生物体内一氧化氮系统的一种功能活跃的 "工作形式"：综述

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S0026893323060183

A. F. Vanin

引用次数: 0

Upregulation of MHC I Antigen Processing Machinery Gene Expression in Breast Cancer Cells by Trichostatin A Trichostatin A 上调乳腺癌细胞中 MHC I 抗原加工机制基因的表达

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-12-01 DOI: 10.1134/S0026893324010151

A. H. Murtadha, N. A. Sharudin, I. I. Azahar, A. T. C. Has, N. F. Mokhtar

引用次数: 0

The Development of SpCas9 Variants with High Specificity and Efficiency Based on the HH Theory 基于HH理论的SpCas9高特异性高效变异的开发

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-11-22 DOI: 10.1134/s0026893324010187

G. H. Wang, C. M. Wang, X. J. Wu, T. Chu, D. W. Huang, J. Li

引用次数: 0

Latent Macrophage and Immature B Cell Lines Generated with Hygromycin-Resistant Murine Gammaherpesvirus 68 Genome Expresses Modest Levels of Viral miRNAs 耐潮霉素小鼠γ疱疹病毒68基因组产生的潜伏巨噬细胞和未成熟B细胞系表达适度水平的病毒mirna

IF 1.2 4区生物学

Molecular Biology Pub Date : 2023-11-22 DOI: 10.1134/s0026893324010138

M. Kara

{"title":"Latent Macrophage and Immature B Cell Lines Generated with Hygromycin-Resistant Murine Gammaherpesvirus 68 Genome Expresses Modest Levels of Viral miRNAs","authors":"M. Kara","doi":"10.1134/s0026893324010138","DOIUrl":"https://doi.org/10.1134/s0026893324010138","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Murine gammaherpesvirus 68 (MHV68) establishes latency mainly in B cells and causes lymphomas reminiscent of human gammaherpesvirus diseases in laboratory mice. To study the molecular mechanism of virus infection and how the viral determinants control cell and eventually cause tumorigenesis, readily available latently infected cell lines are essential. For in vitro MHV68 latency studies, only two cell culture systems have been available. Gammaherpesviruses are known to infect developing B cells and macrophages, therefore we aimed to expand the MHV68 latently infected cell line repertoire. Here, several latently infected immature B cell and macrophage-like cell line clones were generated. Hygromycin-resistant recombinant MHV68 was isolated from a laboratory-made latent cell line, HE2.1, and propagated to develop stable cell lines that carry the viral genome under hygromycin selection. Subclones of these cells lines were analyzed for viral miRNA expression by TaqMan qPCR and assessed for expression of a lytic viral transcript M3. The cell lines maintain the viral genome as an episome shown by the digestion-circularization PCR assay. Latently infected cell lines generated here do not express viral miRNAs higher than the parental cell line. However, these cell lines may provide an alternative tool to study latency mechanisms and miRNA target identification studies.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"57 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0