Molecular Biology最新文献_第8页

Prime-Editing Methods and pegRNA Design Programs 基质编辑方法和 pegRNA 设计程序

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010084

E. V. Mikhaylova, B. R. Kuluev, G. A. Gerashchenkov, D. A. Chemeris, R. R. Garafutdinov, A. R. Kuluev, An. K. Baymiev, Al. K. Baymiev, A. V. Chemeris

{"title":"Prime-Editing Methods and pegRNA Design Programs","authors":"E. V. Mikhaylova, B. R. Kuluev, G. A. Gerashchenkov, D. A. Chemeris, R. R. Garafutdinov, A. R. Kuluev, An. K. Baymiev, Al. K. Baymiev, A. V. Chemeris","doi":"10.1134/s0026893324010084","DOIUrl":"https://doi.org/10.1134/s0026893324010084","url":null,"abstract":"Abstract—It has been 10 years since CRISPR/Cas technology was applied to edit the genomes of various organisms. Its ability to produce a double-strand break in a DNA region specified by the researcher started a revolution in bioengineering. Later, the Base Editing (BE) method was developed. BE is performed via the formation of single-strand breaks by the mutant form of Cas nuclease (nickase), fused with deaminases and other enzymes. It can be used to promote A ( leftrightarrow ) G and C ( leftrightarrow ) T transitions, and a C → G transversion. Just over 3 years ago, a new Prime Editing (PE) variant of CRISPR/Cas was invented. Unlike BE, in PE the nickase is fused with reverse transcriptase, capable of building a new DNA chain using the pegRNA template. The pegRNA consists of an elongated guide RNA with an extra sequence at the 3'-end. Prime editing makes it possible to insert the desired mutations into this extra sequence and to carry out any substitutions and indels of bases without the use of special donor DNA. To date, a number of PE variants have been proposed; they are briefly considered in this review with an emphasis on prime editing of plant genomes. Some attention is also paid to pegRNA design programs, as well as evaluation of the efficiency of the editing. Such a variety of PE techniques is due to the opportunities of high-precision introduction of desired changes with a rather low frequency of off-target mutations in the genomes of various organisms. The relatively low efficiency of prime editing inspires researchers to offer new approaches. There is hope that further development of the technology will improve PE enough to take its rightful place among the genome targeting methods that are suitable for any organisms, and will have a positive impact on the agricultural sector, industrial biotechnologies, and medicine.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"278 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Retrotransposons in Drosophila melanogaster Somatic Tissues 黑腹果蝇体细胞组织中逆转录转座子的调控

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010096

P. A. Milyaeva, I. V. Kukushkina, A. R. Lavrenov, I. V. Kuzmin, A. I. Kim, L. N. Nefedova

引用次数: 0

Structure and Evolution of DNA Transposons of the L31 Superfamily in Bivalves 双壳类动物中 L31 超家族 DNA 转座子的结构与进化

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010114

M. V. Puzakov, L. V. Puzakova

{"title":"Structure and Evolution of DNA Transposons of the L31 Superfamily in Bivalves","authors":"M. V. Puzakov, L. V. Puzakova","doi":"10.1134/s0026893324010114","DOIUrl":"https://doi.org/10.1134/s0026893324010114","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The mobile genetic elements IS630/Tc1/mariner (ITm) are widespread DNA transposons that make a significant contribution to the evolution of eukaryotic genomes. With the start of large-scale application of next-generation sequencing (NGS) technologies and the emergence of many new whole genome sequences of organisms in nucleotide sequence collections, the ITm elements have been identified in most taxa of the eukaryotic tree of life. Although ITm diversity has been studied in detail, new elements are still found, thus expanding the respective DNA transposon group and calling for review of its classification. Bivalve L31 elements were for the first time analyzed in detail to describe their structures, diversity, distribution, and phylogenetic position among the ITm elements. The L31 transposons were found to form an independent superfamily of an ancient origin within the ITm group. Rather high diversity was observed within the L31 clade; i.e., five phylogenetic clusters were identified. In mollusks, the L31 transposons have been detected only in the subclass Autobranchia and predominate in diversity and number in the infraclass Pteriomorphia. A protein encoded by open reading frame 2 (ORF2) was shown to be an integral structural component of almost all full-length L31 elements. The results provide for a better understanding of the evolution of particular ITm transposons. Further study of the L31 transposons in other taxa (cnidarians) and functional investigation of the ORF2 protein product will help to better understand the evolution of DNA transposons, the mechanisms of their horizontal transfer, and their contribution to eukaryotic biodiversity.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"106 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Group of New Hypermethylated Long Non-Coding RNA Genes Associated with the Development and Progression of Breast Cancer 一组与乳腺癌的发生和发展有关的高甲基化长非编码 RNA 新基因

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010035

E. A. Filippova, V. I. Loginov, S. S. Lukina, A. M. Burdennyy, I. V. Pronina, T. P. Kazubskaya, E. A. Braga

{"title":"A Group of New Hypermethylated Long Non-Coding RNA Genes Associated with the Development and Progression of Breast Cancer","authors":"E. A. Filippova, V. I. Loginov, S. S. Lukina, A. M. Burdennyy, I. V. Pronina, T. P. Kazubskaya, E. A. Braga","doi":"10.1134/s0026893324010035","DOIUrl":"https://doi.org/10.1134/s0026893324010035","url":null,"abstract":"Abstract—Breast cancer is the most common type of cancer among women. The study of the mechanisms of metastasis, the main cause of death from breast cancer, as well as the search for new markers for early diagnosis and prognosis of breast cancer, is an extremely topical issue. New perspectives in the diagnosis and treatment of breast cancer are opened by the mechanisms of gene regulation involving non-coding RNAs, in particular, long non-coding RNAs (lncRNAs). In this work, we analyzed the methylation levels of seven lncRNA genes (MEG3, SEMA3B-AS1, HAND2-AS1, KCNK15-AS1, ZNF667-AS1, MAGI2-AS3, and PLUT) by quantitative methyl-specific PCR on a set of 79 paired (tumor/normal) samples of breast cancer. Hypermethylation of all seven lncRNA genes was revealed, and hypermethylation of HAND2-AS1, KCNK15-AS1, MAGI2-AS3, and PLUT was detected in breast cancer for the first time. It was found that the level of methylation of the studied lncRNA genes correlated statistically significantly with the stage of the tumor process, the size of the tumor, and the presence of metastases in the lymph nodes. Thus, methylation of the seven studied lncRNA genes is associated with the development and progression of breast cancer, and these genes can be useful as potential markers in the diagnosis and prognosis of breast cancer.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"133 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationships between Cxcl12, Tweak, Notch1, and Yap mRNA Expression Levels in Molecular Mechanisms of Liver Fibrogenesis 肝纤维化分子机制中 Cxcl12、Tweak、Notch1 和 Yap mRNA 表达水平之间的关系

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010060

E. I. Lebedeva, A. T. Shchastniy, A. S. Babenka, D. A. Zinovkin

{"title":"Relationships between Cxcl12, Tweak, Notch1, and Yap mRNA Expression Levels in Molecular Mechanisms of Liver Fibrogenesis","authors":"E. I. Lebedeva, A. T. Shchastniy, A. S. Babenka, D. A. Zinovkin","doi":"10.1134/s0026893324010060","DOIUrl":"https://doi.org/10.1134/s0026893324010060","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Current data on the molecular mechanisms of liver fibrosis and cirrhosis fail to fully explain all stages of their development. Interactions between individual genes and signaling pathways are known to play an important role in their functions. However, data on their relationships are insufficient and often contradictory. For the first time, mRNA expression of Notch1, Notch2, Yap1, Tweak (Tnfsf12), Fn14 (Tnfrsf12a), Ang, Vegfa, Cxcl12 (Sdf), Nos2, and Mmp-9 was studied in detail at several stages of thioacetamide-induced liver fibrosis in Wistar rats. A factor analysis isolated three factors, which combined highly correlated target genes. The first factor included four genes: Cxcl12 (r = 0.829, p < 0.05), Tweak (r = 0.841, p < 0.05), Notch1 (r = 0.848, p < 0.05), and Yap1 (r = 0.921, p < 0.05). The second factor described the correlation between Mmp-9 (r = 0.791, p < 0.05) and Notch2 (r = 0.836, p < 0.05). The third factor included Ang (r = 0.748, p < 0.05) and Vegfa (r = 0.679, p < 0.05). The Nos2 and Fn14 genes were not included in any of the factors. The gene grouping by mRNA expression levels made it possible to assume a pathogenetic relationship between their products in the development of fibrotic changes due to liver toxicity.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"16 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences 各种波长的紫外线辐射造成细胞 DNA 损伤的光化学过程：生物学后果

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010047

G. Ya. Fraikin, N. S. Belenikina, A. B. Rubin

{"title":"Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences","authors":"G. Ya. Fraikin, N. S. Belenikina, A. B. Rubin","doi":"10.1134/s0026893324010047","DOIUrl":"https://doi.org/10.1134/s0026893324010047","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Photochemical reactions in cell DNA are induced in various organisms by solar UV radiation and may lead to a series of biological responses to DNA damage, including apoptosis, mutagenesis, and carcinogenesis. The chemical nature and the amount of DNA lesions depend on the wavelength of UV radiation. UV type B (UVB, 290–320 nm) causes two main lesions, cyclobutane pyrimidine dimers (CPDs) and, with a lower yield, pyrimidine (6-4) pyrimidone photoproducts (6-4PPs). Their formation is a result of direct UVB photon absorption by DNA bases. UV type A (UVA, 320–400 nm) induces only cyclobutane dimers, which most likely arise via triplet–triplet energy transfer (TTET) from cell chromophores to DNA thymine bases. UVA is much more effective than UVB in inducing sensitized oxidative DNA lesions, such as single-strand breaks and oxidized bases. Of the latter, 8-oxo-dihydroguanine (8-oxodG) is the most frequent, being produced in several oxidation processes. Many recent studies reported novel, more detailed information about the molecular mechanisms of the photochemical reactions that underlie the formation of various DNA lesions. The information is mostly summarized and analyzed in the review. Special attention is paid to the oxidation reactions that are initiated by reactive oxygen species (ROS) and radicals generated by potential endogenous photosensitizers, such as pterins, riboflavin, protoporphyrin IX, NADH, and melanin. The review discusses the role that specific DNA photoproducts play in genotoxic processes induced in living systems by UV radiation of various wavelengths, including human skin carcinogenesis.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"5 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Stability of Bacillus velezensis after Two-Year Exposure in Open Space 露天暴露两年后 Velezensis 杆菌基因组的稳定性

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-03-07 DOI: 10.1134/s0026893324010023

S. V. Fialkina, E. A. Deshevaya, A. L. Rakitin, O. I. Orlov

{"title":"Genome Stability of Bacillus velezensis after Two-Year Exposure in Open Space","authors":"S. V. Fialkina, E. A. Deshevaya, A. L. Rakitin, O. I. Orlov","doi":"10.1134/s0026893324010023","DOIUrl":"https://doi.org/10.1134/s0026893324010023","url":null,"abstract":"Abstract—Spore-forming bacteria have a unique resistance to negative environmental conditions, including aggressive space factors, and are an excellent model for studying adaptation mechanisms and survival strategies at the molecular level. The study analyzed the genome of Bacillus velezensis, which remained viable after a 2-year exposure in outer space on the outer surface of the ISS as part of the Test space experiment. A comparative analysis of the draft genomes of the exhibit strain and the ground control did not reveal significant changes; the average nucleotide identity was 99.98%, which indicates the ability of microorganisms to maintain genome stability in space conditions, due to both increased stress resistance of bacterial spores and efficient operation of the system of repair of accumulated changes. The study of a single nucleotide polymorphism in the genome of B. velezensis revealed nine point substitutions, three of which are in intergenic regions, six in protein-coding genes, three of them are missense mutations, two nucleotide deletions leading to a shift in the reading frame, and one synonymous substitution. The profiles of the housekeeping genes were determined during MLST typing and it was found that the allelic profiles obtained for B. velezensis T15.2 and 924 strains do not correspond to any of the previously described sequence types.The presented results indicate the ability of B. velezensis bacteria to maintain the viability of spores and the integrity of the genome for a long time under extreme conditions of outer space, which is important for the problem of planetary protection, as well as the potential possibility of performing biotechnological processes based on B. velezensis during space exploration.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"278 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin Enhances the Effect of ABT-737 in Acute Monocytic Leukemia THP-1 Cells 褪黑激素能增强 ABT-737 对急性单核细胞白血病 THP-1 细胞的作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-02-01 DOI: 10.1134/s002689332401014x

{"title":"Melatonin Enhances the Effect of ABT-737 in Acute Monocytic Leukemia THP-1 Cells","authors":"","doi":"10.1134/s002689332401014x","DOIUrl":"https://doi.org/10.1134/s002689332401014x","url":null,"abstract":" <h3>Abstract</h3> Melatonin (N-acetyl-5-methoxytryptamine, MEL) is a hormone synthesized by the pineal gland. Due to its oncostatic effect, it can be considered as an antitumor agent and used for combination therapy. ABT-737, a Bcl-2 inhibitor, promotes cell death after treatment with agents that induce pro-apoptotic signals. In the present study, the combined effect of MEL and ABT-737 on changes in proliferative and mitotic activity, mitochondrial membrane potential, intracellular production of reactive oxygen species (ROS), and cytosolic Ca2+ was studied. Moreover, changes in the expression of anti- and pro-apoptotic proteins (Bcl-2 and Bax), autophagy markers (LC3A/B (I, II)), endoplasmic reticulum stress markers (chaperones BIP and PDI, CHOP) were studied under these conditions. The effect of MEL together with ABT-737 led to an increase in the level of cytosolic Ca2+, intracellular production of ROS and a decrease in the membrane potential of mitochondria. The content of Bcl-2 increased, while the level of Bax decreased. Activation of CHOP stimulated autophagy and led to a decrease in the synthesis of chaperones BIP and PDI. It is assumed that melatonin can enhance the effect of other chemotherapeutic agents and can be used in the treatment of tumors. ","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"79 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restraint Stress-Induced Expression of Fos and Several Related Genes in the Hypothalamus of Hypertensive ISIAH Rats 约束应激诱导高血压 ISIAH 大鼠下丘脑中 Fos 和几个相关基因的表达

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-02-01 DOI: 10.1134/s0026893324010072

{"title":"Restraint Stress-Induced Expression of Fos and Several Related Genes in the Hypothalamus of Hypertensive ISIAH Rats","authors":"","doi":"10.1134/s0026893324010072","DOIUrl":"https://doi.org/10.1134/s0026893324010072","url":null,"abstract":" <h3>Abstract</h3> Stress can play a significant role in arterial hypertension and many other complications of cardiovascular diseases. Considerable attention is paid to the study of the molecular mechanisms involved in the body response to stressful influences, but there are still many blank spots in understanding the details. ISIAH rats model the stress-sensitive form of arterial hypertension. ISIAH rats are characterized by genetically determined enhanced activities of the hypothalamic–pituitary–adrenocortical and sympathetic–adrenomedullary systems, suggesting a functional state of increased stress reactivity. For the first time, the temporal expression patterns of Fos and several related genes were studied in the hypothalamus of adult male hypertensive ISIAH rats after a single exposure to restraint stress for 30, 60, or 120 min. Fos transcription was activated and peaked 1 h after the start of restraint stress. The time course of Fos activation coincided with that of blood pressure increase after stress. Activation of hypothalamic neurons also alters the transcription levels of several transcription factor genes (Jun, Nr4a3, Jdp2, and Ppargc1a), which are associated with the development of cardiovascular diseases. Because Fos induction is a marker of brain neuron activation, activation of hypothalamic neurons and an increase in blood pressure were concluded to accompany increased stress reactivity of the hypothalamic–pituitary–adrenocortical and sympathoadrenal systems in hypertensive ISIAH rats during short-term restraint. ","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"56 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stochastic Packaging of Cas Proteins into Exosomes 将 Cas 蛋白随机包装到外泌体中

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-02-01 DOI: 10.1134/s0026893324010102

{"title":"Stochastic Packaging of Cas Proteins into Exosomes","authors":"","doi":"10.1134/s0026893324010102","DOIUrl":"https://doi.org/10.1134/s0026893324010102","url":null,"abstract":"Abstract—CRISPR/Cas systems are perspective molecular tools for targeted manipulation with genetic materials, such as gene editing, regulation of gene transcription, modification of epigenome etc. While CRISPR/Cas systems proved to be highly effective for correcting genetic disorders and treating infectious diseases and cancers in experimental settings, clinical translation of these results is hampered by the lack of efficient CRISPR/Cas delivery vehicles. Modern synthetic nanovehicles based on organic and inorganic polymers have many disadvantages, including toxicity issues, the lack of targeted delivery, and complex and expensive production pipelines. In turn, exosomes are secreted biological nanoparticles that exhibit high biocompatibility, physico-chemical stability, and the ability to cross biological barriers. Early clinical trials found no toxicity associated with exosome injections. In the recent years, exosomes have been considered as perspective delivery vehicles for CRISPR/Cas systems in vivo. The aim of this study was to analyze the efficacy of CRISPR/Cas stochastic packaging into exosomes for several human cell lines. Here, we show that Cas9 protein is effectively localized into the compartment of intracellular exosome biogenesis, but stochastic packaging of Cas9 into exosomes turns to be very low (~1%). As such, stochastic packaging of Cas9 protein is very ineffective and cannot be used for gene editing purposes. Developing novel tools and technologies for loading CRISPR/Cas systems into exosomes is needed.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"278 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140072635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0