Acta Medico-Biotechnica最新文献_第8页

The effects of gabapentin and ethanol on the regulated cell death of astrocytes in primary culture 加巴喷丁和乙醇对原代培养星形胶质细胞调控死亡的影响

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.189

Paulína Hutyrová, Jan Štangelj, M. Lipnik-Štangelj

{"title":"The effects of gabapentin and ethanol on the regulated cell death of astrocytes in primary culture","authors":"Paulína Hutyrová, Jan Štangelj, M. Lipnik-Štangelj","doi":"10.18690/actabiomed.189","DOIUrl":"https://doi.org/10.18690/actabiomed.189","url":null,"abstract":"Purpose: In this study, the effect of gabapentin on the regulated cell death of astrocytes in primary culture was examined. Because astrocytes are relatively resistant to decay by apoptotic pathways, the effect of different concentrations of gabapentin on apoptosis in necroptosis was tested as another form of regulatedcell death. In addition, the impact of gabapentin on the death of astrocytes that were exposed to ethanol was also examined. \u0000Methods: Primary cultures of astrocytes that were obtained from the brain cortex of newborn rats were used as the experimental model. Cells were exposed to different concentrations of gabapentin only, ethanol only or to a combination of ethanol and gabapentin. Using flow cytometry, the proportions of viable, early apoptotic, necroptotic, and secondary dead cells were determined. \u0000Results: The effect of gabapentin on early astrocytic apoptosis and necroptosis was dependent on concentration. In concentrations of up to 10 μg/mL, gabapentin did not affect astrocyte deaths; whereas at higher concentrations, the proportion of necroptotic cells increased. The concomitant exposure of the cells to gabapentin (10 μg/mL) and ethanol (100 mM) for 24 hours did not significantly affect cell death caused by ethanol. For cells that are exposed to 50 mM ethanol for 7 days, gabapentin slightly reduced the proportion of necrotic cells. \u0000Conclusion: Gabapentin did not affect the viability of astrocytes in concentrations up to 10 μg/mL. The concomitant exposure of astrocytes to ethanol and gabapentin for 24 hours did not reduce the toxicity of ethanol. In astrocytes that are chronically exposed to ethanol, gabapentin slightly reduced the effect of ethanol on necroptosis.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127556386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Historical overview of breastfeeding in Slovenia 斯洛文尼亚母乳喂养的历史概况

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.150

Dušanka Mičetić Turk, E. Turk, M. Šikić Pogačar

引用次数: 1

How and why to analyze microbiota – a quick guide for clinicians 如何以及为什么分析微生物群-临床医生的快速指南

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.191

Sabina Horvat, M. Rupnik

引用次数: 1

Varus knee deformity in adolescent soccer players 青少年足球运动员膝内翻畸形

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.173

Z. Krajnc, M. Vogrin

{"title":"Varus knee deformity in adolescent soccer players","authors":"Z. Krajnc, M. Vogrin","doi":"10.18690/actabiomed.173","DOIUrl":"https://doi.org/10.18690/actabiomed.173","url":null,"abstract":"The lower limb axis develops dynamically throughout the first and second decades of life. In recent years, with respect to youth athletes, we have been facing a progressive increase in the intensity of exercise during training and competition (involving increased time, frequency, and power), and, as a result, there hasbeen an increase in the number of acute and chronic overuse injuries. There are some sports with a higher incidence of injuries, one of which is soccer. A review of literature for the past 15 years with a focus on varus knee deformities in adolescent soccer players showed that varus axial deformities of the lower limbsoccur more frequently in soccer players than in the general population and in athletes from other sport, probably due to the specific loading pattern in soccer training. Varus deformities alter knee biomechanics, which results in medial joint space overloading, and consequently an early onset of degenerative joint disease. In addition, bowlegs predisposes athletes to patellofemoral pain syndrome and meniscal lesions. The etiology of genu varum in soccer is thought to be multifactorial, ranging from the natural selection of players to mechanical overload of the proximal medial tibial growth plate. A growing number of clinical reports and animal studies have indicated that intensive sport training may precipitate pathologic changes of the knee joint growth plate, and even produce a growth disturbance.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122111234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laparoscopic cornual resection for interstitial pregnancy - a case report 腹腔镜间质性妊娠角切除术1例

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.179

T. Bizjak, S. Rakić

{"title":"Laparoscopic cornual resection for interstitial pregnancy - a case report","authors":"T. Bizjak, S. Rakić","doi":"10.18690/actabiomed.179","DOIUrl":"https://doi.org/10.18690/actabiomed.179","url":null,"abstract":"Purpose: Ectopic pregnancy is defined as the implantation of a fertilized egg outside of the uterine endometrium. Previous research has reported an increase in the incidence of ectopic pregnancy and estimated that this condition occurs in 1.5 to 2.5% of pregnancies (1). In cases of ectopic pregnancy, the fertilized egg most often implants in the fallopian tubes. In some cases, it can also implant at the site where the fallopian tube and the uterus join; this is a condition referred to as interstitial pregnancy (2). Rupture of interstitial pregnancy can have catastrophic consequences with a mortality rate of up to 2% (2, 3). \u0000Case report: Here, we report the case of a 33-years-old patient with an interstitial pregnancy in the right uterine horn. We successfully performed laparoscopic surgery with a harmonic scalpel and preliminary coagulation of the uterine artery without any complications. Following surgery, the pregnancy was normal and was carried to term. \u0000Conclusions: Previously, the treatment of interstitial pregnancy was mostly performed surgically by open surgery including resection of the affected horn of the uterus (4). However, several successful clinical cases of laparoscopic surgery have now been reported (5-8). In our opinion, laparoscopic resection, with a harmonic scalpel and preliminary coagulation of the uterine artery, represents a safe and efficient techniqueand should be used more regularly in the treatment of interstitial pregnancy.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122537745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlations between infant mortality and recommended immunization schedules against vaccine–preventable diseases in Slovenia 斯洛文尼亚婴儿死亡率与针对疫苗可预防疾病的推荐免疫时间表之间的相关性

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.145

M. Zemljič, B. Pejković, Ivan Kranjc, A. Kraigher

{"title":"Correlations between infant mortality and recommended immunization schedules against vaccine–preventable diseases in Slovenia","authors":"M. Zemljič, B. Pejković, Ivan Kranjc, A. Kraigher","doi":"10.18690/actabiomed.145","DOIUrl":"https://doi.org/10.18690/actabiomed.145","url":null,"abstract":"Purpose: The infant mortality rate (IMR) is among the most important indicators of a nation's health and well–being. In Slovenia, over the last 55 years, infant mortality (IM) has decreased rapidly and vaccinations are undoubtedly the major contributing factor. In Slovenia, infant immunization is initiated at the age of 3 months. The vaccination schedule includes several doses of combined vaccines that should be administered according to the yearly program on immunization. \u0000Methods: In this retrospective study, 279 infants were divided into subgroups in accordance with the vaccination program. The structure of the study design used the data of the National Institute of Public Health of Slovenia regarding the causes of IM for the years 2007–2011 \u0000Results: Direct and indirect leading causes of death were infant respiratory distress syndrome (n = 22), extreme immaturity (n = 19), necrotizing enterocolitis (n = 18), extremely low birth weight (n = 14), and hypoplastic left heart syndrome (n = 14). The maximum IMR (85.30%) occurred before the recommended vaccination period. During the prescribed program of vaccination, the IMR was 14.70%. \u0000Conclusion: Genetic alterations, congenital abnormalities, and preterm birth complications are the major and leading causes of IM. Most instances of IM occur within the first two months of life, before the recommended vaccinations. During the recommended vaccination period, IM is low and causes of death are largely congenital and genetic diseases. The results of the comparative study during the recommended vaccination schedule by the end of the first year of life from 2007 to 2011 substantiates that the vaccines are not a major cause of IM.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125297605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations of the CYP21A2 gene: Association of p.V281L mutation with polycystic ovarian syndrome (PCOS) CYP21A2基因突变:p.V281L突变与多囊卵巢综合征的关系

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.169

Š. Stangler Herodež, L. Fijavž, B. Zagradišnik, M. Došen, I. Takač, N. Kokalj-Vokač

{"title":"Mutations of the CYP21A2 gene: Association of p.V281L mutation with polycystic ovarian syndrome (PCOS)","authors":"Š. Stangler Herodež, L. Fijavž, B. Zagradišnik, M. Došen, I. Takač, N. Kokalj-Vokač","doi":"10.18690/actabiomed.169","DOIUrl":"https://doi.org/10.18690/actabiomed.169","url":null,"abstract":"Purpose: The CYP21A2 gene encodes the enzyme 21–hydroxilase, which is responsible for the production of steroids. These hormones are key mediators of sexual development and conception. Patients with 21–hydroxilase deficiency tend to be affected in different stages of life. The purpose of this study was to compare the genetic profiles of women with unexplained infertility problems with the genetic profiles of healthy controls. Furthermore, were analyzed associations between mutations of the CYP21A2 gene and various clinical and laboratory parameters. \u0000Methods: We enrolled 300 women, diagnosed with unexplained infertility problems, into this retrospective study. For each subject, we recorded important clinical and laboratory parameters during different phases of the menstrual cycle. In the control group, we enrolled 100 samples. Each subject provided a blood sample, which was used to isolate DNA for subsequent polymerase chain reaction (PCR). \u0000Results: In total, 11.6% of the test subjects exhibited significant hormonal deviations (HD) (estrogens and/ or progestins and/or total testosterone) and 5.3% were diagnosed with polycystic ovarian syndrome (PCOS). We identified a significant association between the p.V281L mutation and the frequency of test subjects with significant HD (x2=6.99, p=0.01). A similar association was also observed between p.V281L mutation and the frequency of test subjects with PCOS (x2=16.78, p=0.00). However, we did not establish any associations between the frequency of mutations in test subjects when compared with controls. In addition, we did not find any significance in the frequency of CYP21A2 gene mutations and any of the laboratory parameters tested. \u0000Conclusion: Our results identify a significant association of the p.V281L mutation in the CYP21A2 gene with the frequencies of both PCOS and significant HD.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121078844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the editorial to the Book of Portraits on its Way / Iz uvodnika Knjigi portretov na pot 从《路上的肖像书》的社论/ Iz uvodnika Knjigi portretov na pot

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.156

Ivan Kranjc

{"title":"From the editorial to the Book of Portraits on its Way / Iz uvodnika Knjigi portretov na pot","authors":"Ivan Kranjc","doi":"10.18690/actabiomed.156","DOIUrl":"https://doi.org/10.18690/actabiomed.156","url":null,"abstract":"(The book will come out in the publishing house Faculty of Medicine University of Maribor) \u0000In 2014, on the tenth anniversary of operations of the University of Maribor Faculty of Medicine, we published the monograph \"Ten Years of the Faculty of Medicine of the University of Maribor\". In the monograph, we provided a description of the creation and operation of the second Slovenian medical faculty. Much has happened since the first decade and we have already advanced into the second. An attentive observer would not have missed the important milestones of our development from the establishment of the Bologna Reform to our greatest achievement—building an urgently needed faculty on the right riverbank of the Drava River. Following the ideas of the Architecture Professor Boris Podrecca, the faculty is attached to the city and the university. All along, especially since September 2013, we have monitored the development of our medical studies, introduced new features to the program, and developed or renewed basic research studies. Novel appropriate premises and stateof- the-art medical equipment have been the basis for the formation of numerous innovations and changes.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131858491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic relevance of free light chain indices and their relation to the clinical presentation of multiple sclerosis 游离轻链指数的诊断意义及其与多发性硬化临床表现的关系

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.192

Sanja Karakatič, J. Magdič, S. Karakatič, Tomaž Omerzu, E. Modrič, Tanja Hojs Fabjan

{"title":"Diagnostic relevance of free light chain indices and their relation to the clinical presentation of multiple sclerosis","authors":"Sanja Karakatič, J. Magdič, S. Karakatič, Tomaž Omerzu, E. Modrič, Tanja Hojs Fabjan","doi":"10.18690/actabiomed.192","DOIUrl":"https://doi.org/10.18690/actabiomed.192","url":null,"abstract":"Purpose: Cerebrospinal fluid (CSF) free light chains (FLC) are of promising diagnostic importance in patients with multiple sclerosis (MS). However, research is required to confirm their utility as part of the diagnostic criteria for MS. In addition, very few publications can be found addressing the relationship of FLCs to the clinical presentation at the onset of MS. We aimed to evaluate the diagnostic performance of the FLC kappa and lambda indices, and explored their relationship to the number and type of functional systems (FS) involved. \u0000Methods: FLC indices were determined in paired CSF and serum samples from 71 patients with noninflammatory neurological diseases (non-MS, n = 29) and new-onset MS (MS, n = 42). The latter were further divided into two groups with mono- and polysymptomatic presentation. FS involvement was evaluated in accordance with the Expanded Disability Status Scale (EDSS). \u0000Results: Using both FLC indices along with the age and sex of patients, the diagnostic accuracy of MS was higher (AUC, 0.991; sensitivity, 95.5%; specificity, 95.8%) than that observed when using either the lambda index (AUC, 0.677; sensitivity, 68.0%; specificity, 60.0%) or kappa index (AUC, 0.957; sensitivity, 88.5%; specificity, 88.0%) alone. No differences were observed in the lambda (U = 78.0; p = 0.749) or kappa indices (U = 82.0; p = 0.885) between mono- and polysymptomatic patients. Nor was a correlation found between FLC indices and EDSS scores. \u0000Conslusion: FLC indices could be useful diagnostic markers for MS. However, such indices do not differentiate between clinical presentations at disease onset and are not correlated with baseline EDSS scores.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131344684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Determining high risk for squamous intraepithelial lesion progression in patients with recurrent or persistent HPV 16/18 infection: is there a role for biomarkers? 确定复发性或持续性HPV 16/18感染患者鳞状上皮内病变进展的高风险:生物标志物是否有作用?

Acta Medico-Biotechnica Pub Date : 2021-11-29 DOI: 10.18690/actabiomed.165

D. Arko, M. Sobočan, I. Takač, Suzana Bračič

引用次数: 0