Acta Medico-Biotechnica最新文献

{"title":"Experience involving the implementation and compliance with the ERAS® at a small gynecologic oncology centre","authors":"M. Pakiz, Nives Lonzarič, B. Krebs, Sarah Dobnik, M. Sobočan, Kristina Zadravec","doi":"10.18690/actabiomed.193","DOIUrl":"https://doi.org/10.18690/actabiomed.193","url":null,"abstract":"Purpose: We report on the first patient with refractory hypoxemia treated with venovenous extracorporeal membrane oxygenation (VV ECMO) at the Medical Intensive Care Unit of the University Medical Centre Maribor. \u0000Case report: A 52-year-old male was admitted after successful cardiopulmonary resuscitation from out-of-hospital cardiac arrest (first rhythm was ventricular fibrillation, with 40 min to return of spontaneous circulation). The patient underwent primary percutaneous revascularization of the left anterior descendingcoronary artery with insertion of one coronary stent. Hypoxemia was present during patient transportation and the procedure and continued to worsen after admission. Due to refractory hypoxemia caused by aspiration pneumonia, treatment with the VV ECMO was initiated. The ECMO procedure wasperformed without complications and led to improvement in gas exchange and lung mechanics. The patient was then successfully weaned from ECMO, decannulated, and extubated. After extubation, the patient’s course of treatment was complicated by hospital pneumonia and catheter-related blood stream infection.As his state deteriorated, reintubation was necessary. Despite intensive treatment, the patient died on day 30. \u0000Conclusions: Response to the VV ECMO treatment of refractory hypoxemia due to aspiration pneumonia was positive and without complications. In spite of initial improvement, the patient died after several weeks due to recurring infection and septic shock. ","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127585076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subendocardial viability ratio, ambulatory blood pressure monitoring and serum biomarkers for cardiovascular disease in chronic kidney patients","authors":"Nejc Piko, Tajda Šrot, Nina Hojs, S. Bevc, R. Hojs, R. Ekart","doi":"10.18690/actabiomed.188","DOIUrl":"https://doi.org/10.18690/actabiomed.188","url":null,"abstract":"Purpose: Chronic kidney disease (CKD) is associated with a higher prevalence of cardiovascular diseases. The pulse wave analysis measures the aortic pressure waveform based on noninvasive peripheral pressure recording. The subendocardial viability ratio, as part of the pulse wave analysis, is a noninvasivemeasure of microvascular coronary perfusion. \u0000Methods: In the present study, we investigated the relationship between the subendocardial viability ratio, 24-h ambulatory blood pressure measurements, and serum biomarkers to determine the risk for cardiovascular disease in patients with CKD. A pulse wave analysis using applanation tonometry was performed in 86 patients with CKD. Serum biomarkers were measured in patients, and the patients were grouped according to the median value of the subendocardial viability ratio. \u0000Results: The mean age of the patients was 60 ± 13 years; 65% of the patients were men, and the mean subendocardial viability ratio was 151% ± 34%. Patients with CKD in the lower subendocardial viability ratio group (<151%) had significantly lower hemoglobin (p=0.002) and higher NT-proBNP (p=0.034), serum phosphorus (p=0.04), augmentation index (p=0.003), 24-h pulse (p=0.004), and 24-h pulse pressure(p=0.003). \u0000Conclusion: Our findings suggest that patients with CKD with a subendocardial viability ratio below 151% have a higher cardiovascular risk.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"299 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123659144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Folliculotropic mycosis fungoides: Clinicopathological features","authors":"S. Kovačič","doi":"10.18690/actabiomed.157","DOIUrl":"https://doi.org/10.18690/actabiomed.157","url":null,"abstract":"Folliculotropic variant of mycosis fungoides (MF) is a rare type of cutaneous T-cell lymphoma, detected in less than 10% of patients with MF. It occurs mostly in elderly adults, and usually involves the head, neck region, and upper trunk. The diagnosis of this disease is based on a combination of clinical presentation, histology, and T-cell monoclonality. Identification of this variant of disease is important because it may imply worse prognosis and require more intensive treatment.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114942690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contemporary approach to diagnostics in women with suspected pelvic endometriosis","authors":"J. Knez, A. Dovnik, M. Pakiz, I. But, M. Reljic, V. Gavrič Lovrec, Maja Banović, I. Takač","doi":"10.18690/actabiomed.182","DOIUrl":"https://doi.org/10.18690/actabiomed.182","url":null,"abstract":"Endometriosis is a common chronic female reproductive system disease, characterised by the presence of endometrial tissue outside the endometrium and myometrium. It can severely compromise quality of life and have an impact on fertility. Although advances in conservative medical treatment options have been made, surgery is often a necessary part of treatment for this insidious disease.Historically, it was believed that only ovarian endometriosis could be diagnosed non-invasively by an ultrasound examination, while endometriosis at other locations could not be observed. With advances in imaging techniques over the last decades, this has changed dramatically and today deep pelvic endometriosis can be diagnosed non-invasively with high sensitivity and specificity. The aim of this review is to evaluate the contribution of clinical examination, laparoscopy, and non-invasive imaging techniques, mainly transvaginal ultrasound and magnetic resonance imaging, to diagnose deep pelvic endometriosis.Evidence shows that transvaginal ultrasound with high-quality equipment in experienced hands offers high diagnostic accuracy for deep pelvic endometriosis diagnosis. Comprehensive ultrasound examination should be used as a first-line method of choice in diagnostics of women with suspected endometriosisand chronic pelvic pain.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134408698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyarteritis nodosa in a 6-year-old girl – a case report and overview of current management techniques of the disease in paediatric patients","authors":"N. Marčun Varda, M. Močnik","doi":"10.18690/actabiomed.180","DOIUrl":"https://doi.org/10.18690/actabiomed.180","url":null,"abstract":"Purpose: In this article a rare case report of a 6-year-old girl with polyarteritis nodosa (PAN) is presented and current clinical and treatment recommendations and new research data are reviewed. The latter is needed to clearly describe disease presentation, treatment responses, and prognosis in children. PAN is a rare form of vasculitis in children, affecting medium- and smallsized arteries. Its clinical presentation can be quite unspecific and insidious at the beginning, presenting a diagnostic problem. \u0000Methods: Presentation of the patient clinical characteristics, results of the investigation and treatment carried out, as well as the approach to management, treatment, and monitoring of patients with the same condition. \u0000Results: Patient diagnosis was confirmed by laboratory and morphological diagnostics. Treatment with corticosteroids and mycophenolate mofetil was introduced, which led to clinical improvement. The patient requires further lifelong follow-up. Conclusions: Early diagnosis and timely initiation of treatment are of the utmost importance for good prognosis and prevention of devastating irreversible impairment caused byPAN. New multicentre research is needed to improve the treatment of rare paediatric patients with this condition.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"131 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132179248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early diagnosis of giant cell arteritis - the basis of good outcome","authors":"I. Holc","doi":"10.18690/actabiomed.160","DOIUrl":"https://doi.org/10.18690/actabiomed.160","url":null,"abstract":"Purpose: If untreated, giant cell arteritis can lead to blindness and stroke. The study objectives were to assess diagnostic procedures and treatment in early interventional clinic in University Clinical Centre Maribor in patients with temporal arteritis. \u0000Methods: Retrospective study (from 2012 to 2017) of patients diagnosed with temporal arteritis. We assessed epidemiological data, delay of diagnosis, and diagnostic procedures. Results were assessed with statistical methods (SPSS 22.0). \u0000Main goal: The main goal was to determinate the delay in days between symptom onset and admission to the interventional rheumatology clinic and to assess the causes of delay. \u0000Results: Fifty-three GCA (66 % female) patients with mean age 76.25 (from 63–89 years) years were included. Mean time duration of symptoms before admission to our early interventional clinic was 33.74 (0–180) days. The diagnostic procedure was completed in mean time of 2.04 days from the presentation at our interventional rheumatology clinic. The median time to the temporal artery biopsy (TAB) performed in 52 /53 patients was 2 days, with the median 2 days to the preliminary histological results from admission. TAB was positive in 43 (81.1%) of cases. The median time from admittance to colour Doppler sonography (CDS) of aortic arch branches was 2 days and it was positive in all 19 (35.8%) performed cases. 16 (30.2%) patients had polymyalgia rheumatica, 35 (66%) patients had visual disturbances, permanent one eye blindness occurred in 12 (22.64%) patients, and 2 (2.8%) patients experienced permanent blindness on both eyes. Seventeen patients (32.1%) were initially treated with intravenous methylprednisolone pulse. The mean initial dose of oral methylprednisolone was 45.55 (± 15.54) mg. All patients received low dose Aspirin. \u0000Conclusion: Early diagnosis and treatment of giant cell arteritis are very important as miss- or non-diagnosed GCA can lead to permanent blindness of the patient. With better education and public awareness, better access and better professional education of primary care physicians, and early admission to secondary interventional clinics we might spare these patients from the devastating consequences of the GCA.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114367851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten years after the release of the first issue of Acta medico-biotechnica / 10 let po izidu prve številke revije Acta medico-biotechnica","authors":"D. Pahor","doi":"10.18690/actabiomed.164","DOIUrl":"https://doi.org/10.18690/actabiomed.164","url":null,"abstract":"Dear readers, We almost overlooked this date – the 10th anniversary of the publication of the first issue. We are so used to its half-yearly publication that we simply forgot to count. But the fact is that on December 03, 2008 the first issue of Acta medico-biotechnica was published. Slowly we have taken it as our own, it has become a part of us. The majority of you are naming it just Acta. We have stopped thinking about whether the next issue number will be released or not. In between there were years that we survived with more difficulty, there were even doubts about it continuing, but we have endured and succeeded so that we can now light 10 candles on the cake. Of course, we will, upon this jubilee, also blow them out and at that express wishes to the journal for many further successful years.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121687980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevention of Campylobacter jejuni K49/4 adhesion to porcine small intestine cell line PSI cl1 using different plant extracts","authors":"M. Šikić Pogačar, A. Klančnik, S. Smole Možina, Dušanka Mičetić Turk","doi":"10.18690/actabiomed.154","DOIUrl":"https://doi.org/10.18690/actabiomed.154","url":null,"abstract":"Purpose: The aim of this study was to investigate the in vitro anti–adhesive properties of chemically characterized ethanolic extracts from waste Pinot noir grape (GSS) skins and seeds, olive tree leaves (OE), thyme (Thymus vulgaris) prior to (TE) and its residue after (TE–R) hydrodistillation of the essential oil, as well as Alpinia katsumadai ethanolic seed extracts (SEE) and its hydrodistillation residue (hd- SEE–R) against the pig small intestine epithelial cell line, PSI cl1. \u0000Methods: Using PSI cl1, the anti– adhesion activities of these extracts, which normally represent \"waste material\" and by–products from the agro– food industry, were determined. Initially, the anti–Campylobacter jejuni and cytotoxic activities of GSS, TE, TE–R, SEE and hdSEE–R were determined to avoid any interference in the anti– adhesion assay being used. \u0000Results: The A. katsumadai extracts showed the strongest anti–adhesive activities against C. jejuni K49/4. When using TE and TE–R, C. jejuni adhesion to PSI cl1 cells was inhibited by almost 30% over a large concentration range of extracts. GSS extracts had the lowest impact on the adhesion rate of C. jejuni to PSI cl1 cells. \u0000Conclusion: Our findings suggest that agro–food waste material and many by–products from the agro–food industry represent sources of bioactive phytochemicals that are effective at low concentrations and could be used as therapeutic agents to prevent bacterial adhesion. This represents a step towards the application of new innovative strategies to control Campylobacter contamination and infection in the food chain. We suggest that not only plant extracts, but also waste material and agro–food industry by–products can be used as promising novel therapeutic agents with possible medical and industrial applications.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127266732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preoperative renal dysfunction is a predictor of postoperative atrial fibrillation in coronary artery bypass patients","authors":"Miha Antonič","doi":"10.18690/actabiomed.184","DOIUrl":"https://doi.org/10.18690/actabiomed.184","url":null,"abstract":"Purpose: Atrial fibrillation is the most common arrhythmia following cardiac surgery. An association between preoperative kidney dysfunction and postoperative atrial fibrillation has been previously reported; however, no study has so far investigated the impact of kidney function during cardiopulmonary bypass on the incidence of atrial fibrillation. The aim of this study was to investigate the value of preoperative renal function and urine output during cardiopulmonary bypass as predictors of postoperative atrial fibrillation in patients undergoing elective coronary artery bypass. \u0000Methods: This observational retrospective study included 409 patients who underwent elective coronary artery bypass surgery. According to whether or not atrial fibrillation developed postoperatively, the patients were divided into a sinus rhythm group (n = 340) and a new-onset atrial fibrillation group (n = 69). The impact of preoperative (serum creatinine and estimated glomerular filtration rate) and intraoperative (urine output during cardiopulmonary bypass) kidney function on the incidence of new-onset postoperative atrial fibrillation was assessed using univariable and multivariable analyses. \u0000Results:Patients with atrial fibrillation were older, and had higher EuroSCORE II and higher preoperative B-type natriuretic peptide levels than patients with sinus rhythm. Preoperative renal function was worse (higher serum creatinine level and lower estimated glomerular filtration rate) in the atrial fibrillation group than in the sinus rhythm group. However, there were no differences between the groups in urine output during cardiopulmonary bypass. Multivariable analysis identified older age, left ventricular ejection fraction ≤30%, and lower preoperative estimated glomerular filtration rate as independent risk factors for new-onset postoperative atrial fibrillation. \u0000Conclusion: Preoperative renal function—but not urine output during cardiopulmonary bypass—is a predictor of new-onset postoperative atrial fibrillation in patients undergoing elective coronary artery bypass surgery.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129942108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Technical Errors and Construction Optimization for Custom Tissue Arrays at Transplanting and Casting","authors":"Miha Munda","doi":"10.18690/actabiomed.161","DOIUrl":"https://doi.org/10.18690/actabiomed.161","url":null,"abstract":"Purpose: The purpose of this research is to identify causes for the most common typical defects in the production of tissue arrays at the most crucial stages, and to optimize protocols for custom-made tissue arrays at variable input parameters and different final requirements. In this way, we can avoid difficult repairs of tissue arrays, at which, because of re-allocation, major losses can occur, including destruction of the sample due to mechanical and thermal effects. \u0000Methods: Seven hundred and twenty tissue array blocks were included, some for clients and others for experimentation and protocol refinement. Human and animal tissue were used. We tested and evaluated configurations, structural stability, consistency, stability, and practical usability of such designed tissue arrays under different laboratory physical conditions. \u0000Results: We recognized and analyzed 12 mistakes in the production of tissue arrays in the histology laboratory during the stages of transplantation and casting. At each examined error we found the causes, gave suggestions for prevention, and provided instructions on how to eliminate or prevent those causes. \u0000Conclusion: Problems have solutions that are technically feasible but they require individual adjustments of laboratory procedures, time, and learning. This is more difficult or even impossible for automated laboratories, as in the described handmade tissue arrays. However, all laboratories need to produce with maximum quality and precision, which is possible only with addressing problems regularly. The results of this study will contribute to the optimization of custom made tissue array processes.","PeriodicalId":186880,"journal":{"name":"Acta Medico-Biotechnica","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126385472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}