Ministry of Science and Technology, Vietnam最新文献_第7页

Research of newborn screening of Glucose-6-phosphate dehydrogenase (G6PD) deficiency at Medlatec General Hospital Medlatec 综合医院对新生儿葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症筛查的研究

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).06-09

Thi Lan Anh Ta, Thi Que Trinh, T. Vu, Thi Thu Hien Dao, Thi Ngoc Lan Hoang, Thi Kim Phuong Doan

{"title":"Research of newborn screening of Glucose-6-phosphate dehydrogenase (G6PD) deficiency at Medlatec General Hospital","authors":"Thi Lan Anh Ta, Thi Que Trinh, T. Vu, Thi Thu Hien Dao, Thi Ngoc Lan Hoang, Thi Kim Phuong Doan","doi":"10.31276/vjst.65(12).06-09","DOIUrl":"https://doi.org/10.31276/vjst.65(12).06-09","url":null,"abstract":"Objective: Survey some factors affecting Glucose-6-phosphate dehydrogenase (G6PD) enzyme activity from newborn heel blood samples and analyse screening results of some cases of G6PD enzyme deficiency. Research method: Retrospective and prospective cross-sectional description combined with case description. Results: Research on 3223 children who performed the newborn heel blood screening test from September 2022 to September 2023 showed that the high-risk rate of G6PD enzyme deficiency in boys is significantly higher than in girls. The difference is statistically significant when comparing the proportion of neonates with high-risk screening results in the Muong ethnic group and other ethnic minorities compared to the Kinh ethnic group (p<0.01). Between different sampling age groups and different weight groups, the proportion of children with enzyme activity at the high-risk threshold for G6PD enzyme deficiency did not have a statistically significant difference (p>0.05). The mutation encountered in the study was G6PD Union (c.1360C>T) (p.Arg454Cys), the enzyme activity decreased by <10% corresponding to the level of class II enzyme deficiency, showing mild hemolysis and no severe consequences in infancy. Conclusion: Children's gender and ethnicity are related to enzyme activity at risk thresholds for G6PD enzyme deficiency. Studying the correlation between genotype and phenotype is of great significance in genetic counselling, predicting prognosis and managing G6PD enzyme deficiency.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"22 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on rapid multiplication of two hybrid Arabica coffee varieties F1 Centroamericano (H1) and Mundo Maya (H16) through somatic embryogenesis and Bioreactor technology 通过体细胞胚胎发生和生物反应器技术快速繁殖两个阿拉比卡杂交咖啡品种 F1 Centroamericano (H1) 和 Mundo Maya (H16) 的研究

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).56-63

N. G. Khong, Thi Nhu Le

{"title":"Study on rapid multiplication of two hybrid Arabica coffee varieties F1 Centroamericano (H1) and Mundo Maya (H16) through somatic embryogenesis and Bioreactor technology","authors":"N. G. Khong, Thi Nhu Le","doi":"10.31276/vjst.65(12).56-63","DOIUrl":"https://doi.org/10.31276/vjst.65(12).56-63","url":null,"abstract":"In this paper, the research focuses on determining some components and culture conditions in order to optimise the 4 main steps of somatic embryogenesis and embryo regeneration by Bioreactor of two Arabica F1 coffee varieties H1 and H16, including: (1) formation of embryogenic callus, (2) multiplication of embryogenic cells, (3) embryonic differentiation, and (4) regeneration of somatic embryo using the temporary immersion Bioreactor RITA 1L. The results showed that on the medium supplemented with Kinetin 2 mg/ll and Phytagel 4 g/l, the callus formation rate reached 94.2 and 92.1% for 2 varieties H1 and H16, respectively. Besides, the addition of Glycine 20 mg/l to the culture medium increased the embryogenic callus rate by 58.1% in both varieties. Adding Trichostatin A 0.03 mg/l in the embryo differentiation medium in the first 7 days of culture showed that the number of torpedo embryos was scored from 1g of initial callus up to 1287 in H1 and 1635 in H16. Finally, using Bioreactor 1L-RITA, soaking for 1 min every 6 hours, the regeneration efficiency reached 75.02% in H1 and 82.03% in H16.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"13 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of the composition of hookworm species (Ancylostoma duodenale, Ancylostoma ceylanicum)and Necator americanus in humans at community in Tay Ninh province by biomolecular technique 利用生物分子技术确定西宁省社区中人体内钩虫种类（十二指肠肛门疽、塞兰肛门疽）和美洲钩虫的组成成分

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).23-27

Duc Vinh Le, Trinh Vuong Tran, Kim Thach Nguyen

{"title":"Determination of the composition of hookworm species (Ancylostoma duodenale, Ancylostoma ceylanicum)and Necator americanus in humans at community in Tay Ninh province by biomolecular technique","authors":"Duc Vinh Le, Trinh Vuong Tran, Kim Thach Nguyen","doi":"10.31276/vjst.65(12).23-27","DOIUrl":"https://doi.org/10.31276/vjst.65(12).23-27","url":null,"abstract":"Hookworm infections are common in Vietnam and are caused by two known pathogens: Necator americanus and Ancylostoma duodenale. Recent studies have identified a new hookworm species - Ancylostoma ceylanicum, which typically parasitises in dogs. To determine the prevalence of these three hookworm species: A. duodenale, A. ceylanicum, and N. americanus, nested PCR and gene sequencing were employed in fecal samples from suspected individuals residing in Thanh Binh commune, Tan Bien district, Tay Ninh province. Larvae were collected and identified by morphology before 65 DNA samples were extracted and analysed using molecular biology techniques. Results showed that 67.7% (44/65) of the samples were infected with N. americanus, while 16.9% (11/65) and 1.6% (1/65) were infected with A. ceylanicumand A. duodenale, respectively. Co-infection was present in 13.8% of cases, with A. ceylanicum and N. americanus being the most common combination. The origin of the N. americanus species identified was found to be closely related to that reported in Malaysia, while A. ceylanicum was more closely related to the species reported in Thailand. In conclusion, the study found that the new hookworm species A. ceylanicum was more prevalent than the traditional species A. duodenale in Thanh Binh commune (95.2 vs 4.8% of hookworm infections).","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of α-thalassemia mutations by Multiplex ligation-dependent probe amplification 通过多重连接依赖性探针扩增检测α地中海贫血突变

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).01-05

T. Le, Vu Viet Ha Vuong, Van Khanh Tran

引用次数: 0

Isolation and in silico analysis of a transcript variant for a putative terpene synthase (HbTPS6L-X1) from the bark tissues of rubber Hevea brasiliensis RRIV 209 从橡胶 Hevea brasiliensis RRIV 209 树皮组织中分离出推测的萜烯合成酶（HbTPS6L-X1）的转录本变体并对其进行硅学分析

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).70-76

Thi Diem Huong Tran, Ngoc Tuyet Nguyen, Thi Hong Thuong Nguyen

{"title":"Isolation and in silico analysis of a transcript variant for a putative terpene synthase (HbTPS6L-X1) from the bark tissues of rubber Hevea brasiliensis RRIV 209","authors":"Thi Diem Huong Tran, Ngoc Tuyet Nguyen, Thi Hong Thuong Nguyen","doi":"10.31276/vjst.65(12).70-76","DOIUrl":"https://doi.org/10.31276/vjst.65(12).70-76","url":null,"abstract":"Hevea brasiliensis is the only tree species grown to produce natural rubber. The method of harvesting latex by slicing a groove into the bark of the tree mimics a mechanical wounding. In addition, rubber trees could be frequently exposed to pest and pathogen attacks through the scraped bark region. Terpenoids are a group of specialised compounds that play an important role in plant-environment interactions, especially in plant response to stress factors. The diversity of terpenoids is mainly determined by enzymes of the terpene synthase (TPS) family. Screening the RNA-seq data of H. brasiliensis revealed the presence of many putative TPS genes expressed in the bark tissues of rubber trees; however, their function has not been studied. This paper presents the identification of a TPS gene transcript variant (designated as HbTPS6L-X1) from the bark tissues of H. brasiliensis RRIV 209. Through the analysis of the phylogenetic tree, the presence of conserved motifs, and the signal peptide sequence region of the protein, as well as structural homology to known sesquiterpene synthases from other plants, HbTPS6L-X1 was predicted to belong to the TPS-a subfamily, be localised in the cytoplasm and catalyse the conversion of farnesyl diphosphate (FPP) to sesquiterpenes. These in silico functional characteristics are the basis for designing experiments to confirm the in planta biological function of HbTPS6L-X1 protein.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of demecolcine and oocyte maturation time on the efficiency in the production of Vietnamese pot-bellied pig embryos 地美孕素和卵母细胞成熟时间对越南大肚猪胚胎生产效率的影响

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).38-42

T. A. Hoang, Thi Kim Yen Pham, Thi Thu Huong Vu, Van Dat Le, Thi Lan Anh Nguyen, Thi Thanh Nhan Giang, Doan Lan Pham, Q. M. Luu, Khanh Van Nguyen

{"title":"Effects of demecolcine and oocyte maturation time on the efficiency in the production of Vietnamese pot-bellied pig embryos","authors":"T. A. Hoang, Thi Kim Yen Pham, Thi Thu Huong Vu, Van Dat Le, Thi Lan Anh Nguyen, Thi Thanh Nhan Giang, Doan Lan Pham, Q. M. Luu, Khanh Van Nguyen","doi":"10.31276/vjst.65(12).38-42","DOIUrl":"https://doi.org/10.31276/vjst.65(12).38-42","url":null,"abstract":"The results of this study showed that the concentration of Demecolcine affected efficiency in the production of Vietnamese pot-bellied pig embryos. The 4 μM Demecolcine group resulted in a greater oocyte extrusion cones rate in comparison to 0, 6, and 8 μM groups (72.03 vs 1.98%, 59.42, and 51.01%, p<0.05, respectively). The cleavage rate and blastocyst rate in the 4 μM Demecolcine group were significantly higher than in the 6 and 8 μM groups (p<0.05). Demecolcine treatment time affected efficiency in the production of Vietnamese pot-bellied cloned pig embryos. The cleavage and blastocyst rates of the 1 h group were significantly higher than that of the 0.5, 2 and 3 h (84.68 vs 70.92%, 73.86, and 60.36%; 24.91 vs 13.62%, 17.26 vs 10.63%, p<0.05, respectively). The oocyte maturation time of porcine oocytes affected the production efficiency of Vietnamese pot-bellied cloned pig embryos. The oocyte extrusion cone rate of the 20 h group was significantly higher than that of the 18 and 22 h groups (85.34 vs 60.04, and 72.03%, p<0.05, respectively). The cleavage rate and blastocyst rate in the 18 h group were significantly lower than that in the 20 and 22 h groups (66.12 vs 85.42, and 84.68%; 13.71 vs 25.48, and 24.91%, p<0.05, respectively). In conclusion, Demecolcine and oocyte maturation time had effects on the efficiency in the production of Vietnamese pot-bellied pig embryos.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"24 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of estradiol and progesterone contents of Bach Thao and Boer goats during the oestrous cycle 评估勃氏山羊和波尔山羊发情周期中的雌二醇和孕酮含量

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).52-55

Thi Thu Hien Nguyen

引用次数: 0

A model of entrepreneurial intention of graduates in the Mekong river delta 湄公河三角洲毕业生创业意向模型

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-20 DOI: 10.31276/vmostjossh.65(3).66-75

Tran Thi My Phuong, Phan Anh Tu

{"title":"A model of entrepreneurial intention of graduates in the Mekong river delta","authors":"Tran Thi My Phuong, Phan Anh Tu","doi":"10.31276/vmostjossh.65(3).66-75","DOIUrl":"https://doi.org/10.31276/vmostjossh.65(3).66-75","url":null,"abstract":"This article aims to investigate the determinants of the inclination of Mekong river delta (MRD) graduates towards entrepreneurship, with the purpose of providing viable solutions to authorities to enhance the efficacy of entrepreneurial endeavours. The analysis relied on primary data derived from a survey conducted in 2021, which involved 341 graduates from six provinces within the MRD. Various statistical techniques were employed to analyse the data, including the Cronbach’s Alpha test, exploratory factor analysis (EFA), and linear regression analysis. The findings reveal that the entrepreneurial intention of these graduates is positively influenced by a multitude of factors, such as behaviour control, subjective norms, attitude towards entrepreneurship, and perceived desirability, while being negatively impacted by the propensity to act. The study’s primary contribution is the provision of an updated and cohesive framework that enhances understanding of the entrepreneurial intention among graduates in the MRD. The author offers actionable recommendations for various stakeholders: policymakers, universities, entrepreneurs, and researchers.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"15 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green economic model adapting to climate change and disaster prevention in Bach Long Vi island district, Hai Phong city 海防市 Bach Long Vi 岛区适应气候变化和预防灾害的绿色经济模式

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-20 DOI: 10.31276/vmostjossh.65(3).31-41

Xuan Sinh Le, Van Bach Nguyen, Thi Minh Hien Bui, Manh Hao Do, Van Thao Nguyen, Thi Thu Ha Nguyen, Van Tung Pham, Truong Huynh Nguyen

{"title":"Green economic model adapting to climate change and disaster prevention in Bach Long Vi island district, Hai Phong city","authors":"Xuan Sinh Le, Van Bach Nguyen, Thi Minh Hien Bui, Manh Hao Do, Van Thao Nguyen, Thi Thu Ha Nguyen, Van Tung Pham, Truong Huynh Nguyen","doi":"10.31276/vmostjossh.65(3).31-41","DOIUrl":"https://doi.org/10.31276/vmostjossh.65(3).31-41","url":null,"abstract":"In recent years, the economic model in Bach Long Vi island district has been maintained on a small scale, spontaneously, and heavily influenced by natural factors. Additionally, the impacts of climate change and the degradation of natural resources and the environment are increasing burdens on local socio-economic development. Therefore, this study aims to examine and build a compatible green economic model to help adapt to climate change and prevent natural disasters in the island district. The authors have researched and built a green economic model with three main activities (fishery logistics, tourism, and aquaculture), helping economic growth, improving people’s quality of life, and minimising the negative impact on the ecological system. The effectiveness of the green economic model is evaluated through a set of criteria with many contents, such as economic efficiency, the ability to create jobs and improve people’s living standards, and the level of renewable energy use, ecological and environmental conservation. In the end, groups of general and specific solutions are proposed to help strengthen the development and implementation of green economic models in the island district, ensuring adaptation to climate change, disaster prevention, and assurance of efficiency and sustainability for the environment, economy, and society.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"42 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A computational approach to transparency in corporate governance across borders 跨国公司治理透明度的计算方法

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-20 DOI: 10.31276/vmostjossh.65(3).51-65

Wil Martens, Diu Thi Huong Pham, Justin Matthew Pang

{"title":"A computational approach to transparency in corporate governance across borders","authors":"Wil Martens, Diu Thi Huong Pham, Justin Matthew Pang","doi":"10.31276/vmostjossh.65(3).51-65","DOIUrl":"https://doi.org/10.31276/vmostjossh.65(3).51-65","url":null,"abstract":"This article delves into the intricate relationship between information asymmetry and financial reporting comparability, with a particular emphasis on earnings management (EM) in cross-border corporate governance settings. Utilising data from 2,475 non-financial firms across 19 frontier markets from 2003 to 2019, the study employs pooled OLS, fixed effects, and between-effects models to scrutinise the impact of factors like financial comparability, reputable auditing, analyst coverage, and legal systems on earnings management. The findings reveal that enhanced financial comparability, facilitated by strong governance mechanisms such as reputable auditors and analyst coverage, leads to a reduction in earnings management and information asymmetry. Interestingly, leverage does not serve as a constraining factor as commonly believed. The results contribute to the growing body of literature on the application of data science, and challenge the pecking order theory (POT), while lending support to the knowledge-based view (KBV)and convergence theories, thereby offering valuable insights into the role of corporate governance in mitigating information asymmetry.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"142 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139170398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0