Ministry of Science and Technology, Vietnam最新文献_第6页

In vitro propagation of Philodendron hederaceum “Lemon Lime” 试管繁殖 Philodendron hederaceum "Lemon Lime" （柠檬柠条

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).64-69

Thi Thao Ninh, Hong Thuong Nguyen, Truong Son Dinh, Thi Dung Pham, Thi Hue Nong

引用次数: 0

Results of laparoscopic Heller-Toupet operation for achalasia 腹腔镜海勒-图佩特手术治疗贲门失弛缓症的效果

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).15-18

Dang Hung Nguyen, Duc Huan Pham, Hoang Nguyen

{"title":"Results of laparoscopic Heller-Toupet operation for achalasia","authors":"Dang Hung Nguyen, Duc Huan Pham, Hoang Nguyen","doi":"10.31276/vjst.65(12).15-18","DOIUrl":"https://doi.org/10.31276/vjst.65(12).15-18","url":null,"abstract":"Objectives: This study was carried out to evaluate surgical outcomes of laparoscopic Heller-Toupet surgery for achalasia. Methodology: This is a retrospective case series implemented at the Gastrointestinal Department, Hanoi Medical University Hospital between January 2015 and August 2022, including 71 patients with achalasia who underwent laparoscopic Heller-Toupet. Results: 52.1% of patients in the study are male (more than female 47.9%). The average age of the cohort was 44.9±13.9 years. The incidence of dysphagia was 100%. Most patients report esophageal dilatation grade II (76.1%) and disease severity stage I (57.7%). The mean muscle opening length was 7.4±1.3 cm, the operative time was 135±25 minutes. There was no conversion, no intra-and postoperative adverse events. Long-term outcomes: good (no or very mild dysphagia) was 90.1%, moderate (moderate dysphagia) was 9.9% and clinical incidence of reflux syndrome was 17%. There was no case requiring intervention or re-operation. Conclusions: Laparoscopic Heller-Toupet is a safe, low rate of intra- and postoperative complication, and brings good long-term outcomes in the treatment of achalasia.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and paraclinical characteristics of acute bronchitis patients at Hanoi Hospital of Traditional Medical 河内传统医学医院急性支气管炎患者的临床和辅助临床特征

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).19-22

Thi Ngoan Vu, Quynh Hoa Ngo

引用次数: 0

Clinical features, paraclinical characteristics, and etiology of mediastinal bleeding diffusion in children at the Vietnam National Children’s Hospital from 2018 to 2023 2018年至2023年越南国家儿童医院儿童纵隔出血弥漫的临床特征、副临床特征和病因

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).10-14

Thi Le Nguyen, Thi Phuong Thanh Hoang, Thi Thu Hang Hoang, Tuan Hung Phan

{"title":"Clinical features, paraclinical characteristics, and etiology of mediastinal bleeding diffusion in children at the Vietnam National Children’s Hospital from 2018 to 2023","authors":"Thi Le Nguyen, Thi Phuong Thanh Hoang, Thi Thu Hang Hoang, Tuan Hung Phan","doi":"10.31276/vjst.65(12).10-14","DOIUrl":"https://doi.org/10.31276/vjst.65(12).10-14","url":null,"abstract":"Background: In modern life, diffuse alveolar haemorrhage (DAH) is a serious and rare condition in children. Due to the subtle clinical manifestations and diverse causes of the disease, it is easy to be missed or diagnosed late. This study describes clinical and paraclinical characteristics, with the aim to find signs suggesting early diagnosis as well as determine the cause of DAH in children. Objects and methods: Case series. Results: From January 2018 to March 2023, there were 14 children diagnosed with DAH. The age of onset is 4.08 (1.58-5.41) years old, but most patients are diagnosed late with age at diagnosis being 5.25 (1.67-6.16) years old, male/female ratio is 1:1.8. The most common symptoms reported were anaemia (100%), hemoptysis (57.1%), shortness of breath (71.4%), and respiratory failure (85.7%). The common X-ray finding was diffuse infiltrates in both lungs (92.9%). On chest computed tomography scans, 92.9% of patients exhibited images of blurred opacity spreading, 57.1% showed interlobar pleural thickening, and 28.6% presented with images of lung consolidation. 3 patients (22%) had milk allergy, 2 patients (14%) had ANCA (Anti-neutrophil cytoplasmic antibody) vasculitis, and 9 patients (64%) had idiopathic pulmonary haemosiderosis (IPH).","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication and evaluation of some characteristics of the skin's dermis gel direction to application in regenerative medicine 制作和评估皮肤真皮层凝胶的一些特征，以便应用于再生医学

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).34-37

Le Bao Ha Tran, Nguyen Vu Doan, Thi Ngoc My Nguyen, Minh Quan To, Thi Vy Tuyet Le, Thuan Nho Nguyen, Thanh Vy Truong, Thi Hong Tham Nguyen, Thi Thanh Tam Vu, H. Phan, Quang Tri Le

{"title":"Fabrication and evaluation of some characteristics of the skin's dermis gel direction to application in regenerative medicine","authors":"Le Bao Ha Tran, Nguyen Vu Doan, Thi Ngoc My Nguyen, Minh Quan To, Thi Vy Tuyet Le, Thuan Nho Nguyen, Thanh Vy Truong, Thi Hong Tham Nguyen, Thi Thanh Tam Vu, H. Phan, Quang Tri Le","doi":"10.31276/vjst.65(12).34-37","DOIUrl":"https://doi.org/10.31276/vjst.65(12).34-37","url":null,"abstract":"Objective:The tight connection between the cell-extracellular matrix (ECM - Extracellular matrix) is the foundation of tissue development and repair. The extracellular framework simulates the biological properties of organism-based ECM. The ECM of the skin’s dermis is a natural material, containing ingredients ideal for creating products that help tissue heal. This study focuses on the production of gel products from human skin ECM. The skin ECM is gelled by dissolving in a pepsin enzyme solution.Method: 3D gel formation process using neutral enzyme solution and incubation under physiological conditions. Gel samples were evaluated for speed and formability. Additionally, the dermal gel preparation was evaluated for its effects on the survival and proliferation of human fibroblasts in vitro by toxicity testing and proliferation testing, for 10 days. Results: Direct and extract toxicity assessments showed that the ECM gel regimen was safe for fibroblast survival. At the same time, fibroblasts had proliferation properties after 10 days in culture conditions with gel extract.Conclusion: These results showed that the ECM gel product regimen has the ability to positively impact the biological properties of cells and has the potential for application in the field of regenerative medicine.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in basic water environmental parameters of the water intake of brackish water shrimp farming in Northern Vietnam period 2017-2021 2017-2021 年越南北部咸水虾养殖取水口基本水环境参数的变化

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).43-51

H. N. Nguyen, Trong Binh Phan, Thi Minh Nguyet Nguyen, Duc-Binh Nguyen, T. Pham, Thi Thanh Pham

{"title":"Changes in basic water environmental parameters of the water intake of brackish water shrimp farming in Northern Vietnam period 2017-2021","authors":"H. N. Nguyen, Trong Binh Phan, Thi Minh Nguyet Nguyen, Duc-Binh Nguyen, T. Pham, Thi Thanh Pham","doi":"10.31276/vjst.65(12).43-51","DOIUrl":"https://doi.org/10.31276/vjst.65(12).43-51","url":null,"abstract":"This study synthesised and analysed water environmental parameters data of water supply of brackish water shrimp farming in Northern Vietnam over 5 years from 2017 to 2021 to find out the changing patterns of the parameters, thereby making appropriate recommendations for stakeholders. There were 7 analysed and evaluated water environment parameters, including water temperature, dissolved oxygen (DO), salinity, pH, alkalinity, chemical oxygen demand (COD), nitrite (N-NO2), with monitoring frequency of 2 times/month, 10 months/year at 13 monitoring locations for brackish water shrimp farming in 6 provinces: Nam Dinh, Nghe An, Ha Tinh, Quang Binh, Quang Tri, and Thua Thien Hue. Water temperature was low at the beginning of the year, gradually increased to the highest level in June, then gradually decreased to the end of the year. The temperature fluctuated strongly at the beginning and end of the year and tended to increase gradually from the North to the South. DO tended to decrease from the beginning of the year to the end of the year. Salinity was lower in the rainy season, especially in Nghe An and Quang Tri, compared to the others. The pH and alkalinity levels were suitable for brackish shrimp farming. COD in Ha Tinh and Nam Dinh, NO2 in Nam Dinh were often high, indicating that the supply water was contaminated with organic matter and needed a long-term solution.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research of newborn screening of Glucose-6-phosphate dehydrogenase (G6PD) deficiency at Medlatec General Hospital Medlatec 综合医院对新生儿葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症筛查的研究

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).06-09

Thi Lan Anh Ta, Thi Que Trinh, T. Vu, Thi Thu Hien Dao, Thi Ngoc Lan Hoang, Thi Kim Phuong Doan

{"title":"Research of newborn screening of Glucose-6-phosphate dehydrogenase (G6PD) deficiency at Medlatec General Hospital","authors":"Thi Lan Anh Ta, Thi Que Trinh, T. Vu, Thi Thu Hien Dao, Thi Ngoc Lan Hoang, Thi Kim Phuong Doan","doi":"10.31276/vjst.65(12).06-09","DOIUrl":"https://doi.org/10.31276/vjst.65(12).06-09","url":null,"abstract":"Objective: Survey some factors affecting Glucose-6-phosphate dehydrogenase (G6PD) enzyme activity from newborn heel blood samples and analyse screening results of some cases of G6PD enzyme deficiency. Research method: Retrospective and prospective cross-sectional description combined with case description. Results: Research on 3223 children who performed the newborn heel blood screening test from September 2022 to September 2023 showed that the high-risk rate of G6PD enzyme deficiency in boys is significantly higher than in girls. The difference is statistically significant when comparing the proportion of neonates with high-risk screening results in the Muong ethnic group and other ethnic minorities compared to the Kinh ethnic group (p<0.01). Between different sampling age groups and different weight groups, the proportion of children with enzyme activity at the high-risk threshold for G6PD enzyme deficiency did not have a statistically significant difference (p>0.05). The mutation encountered in the study was G6PD Union (c.1360C>T) (p.Arg454Cys), the enzyme activity decreased by <10% corresponding to the level of class II enzyme deficiency, showing mild hemolysis and no severe consequences in infancy. Conclusion: Children's gender and ethnicity are related to enzyme activity at risk thresholds for G6PD enzyme deficiency. Studying the correlation between genotype and phenotype is of great significance in genetic counselling, predicting prognosis and managing G6PD enzyme deficiency.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on rapid multiplication of two hybrid Arabica coffee varieties F1 Centroamericano (H1) and Mundo Maya (H16) through somatic embryogenesis and Bioreactor technology 通过体细胞胚胎发生和生物反应器技术快速繁殖两个阿拉比卡杂交咖啡品种 F1 Centroamericano (H1) 和 Mundo Maya (H16) 的研究

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).56-63

N. G. Khong, Thi Nhu Le

{"title":"Study on rapid multiplication of two hybrid Arabica coffee varieties F1 Centroamericano (H1) and Mundo Maya (H16) through somatic embryogenesis and Bioreactor technology","authors":"N. G. Khong, Thi Nhu Le","doi":"10.31276/vjst.65(12).56-63","DOIUrl":"https://doi.org/10.31276/vjst.65(12).56-63","url":null,"abstract":"In this paper, the research focuses on determining some components and culture conditions in order to optimise the 4 main steps of somatic embryogenesis and embryo regeneration by Bioreactor of two Arabica F1 coffee varieties H1 and H16, including: (1) formation of embryogenic callus, (2) multiplication of embryogenic cells, (3) embryonic differentiation, and (4) regeneration of somatic embryo using the temporary immersion Bioreactor RITA 1L. The results showed that on the medium supplemented with Kinetin 2 mg/ll and Phytagel 4 g/l, the callus formation rate reached 94.2 and 92.1% for 2 varieties H1 and H16, respectively. Besides, the addition of Glycine 20 mg/l to the culture medium increased the embryogenic callus rate by 58.1% in both varieties. Adding Trichostatin A 0.03 mg/l in the embryo differentiation medium in the first 7 days of culture showed that the number of torpedo embryos was scored from 1g of initial callus up to 1287 in H1 and 1635 in H16. Finally, using Bioreactor 1L-RITA, soaking for 1 min every 6 hours, the regeneration efficiency reached 75.02% in H1 and 82.03% in H16.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of the composition of hookworm species (Ancylostoma duodenale, Ancylostoma ceylanicum)and Necator americanus in humans at community in Tay Ninh province by biomolecular technique 利用生物分子技术确定西宁省社区中人体内钩虫种类（十二指肠肛门疽、塞兰肛门疽）和美洲钩虫的组成成分

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).23-27

Duc Vinh Le, Trinh Vuong Tran, Kim Thach Nguyen

{"title":"Determination of the composition of hookworm species (Ancylostoma duodenale, Ancylostoma ceylanicum)and Necator americanus in humans at community in Tay Ninh province by biomolecular technique","authors":"Duc Vinh Le, Trinh Vuong Tran, Kim Thach Nguyen","doi":"10.31276/vjst.65(12).23-27","DOIUrl":"https://doi.org/10.31276/vjst.65(12).23-27","url":null,"abstract":"Hookworm infections are common in Vietnam and are caused by two known pathogens: Necator americanus and Ancylostoma duodenale. Recent studies have identified a new hookworm species - Ancylostoma ceylanicum, which typically parasitises in dogs. To determine the prevalence of these three hookworm species: A. duodenale, A. ceylanicum, and N. americanus, nested PCR and gene sequencing were employed in fecal samples from suspected individuals residing in Thanh Binh commune, Tan Bien district, Tay Ninh province. Larvae were collected and identified by morphology before 65 DNA samples were extracted and analysed using molecular biology techniques. Results showed that 67.7% (44/65) of the samples were infected with N. americanus, while 16.9% (11/65) and 1.6% (1/65) were infected with A. ceylanicumand A. duodenale, respectively. Co-infection was present in 13.8% of cases, with A. ceylanicum and N. americanus being the most common combination. The origin of the N. americanus species identified was found to be closely related to that reported in Malaysia, while A. ceylanicum was more closely related to the species reported in Thailand. In conclusion, the study found that the new hookworm species A. ceylanicum was more prevalent than the traditional species A. duodenale in Thanh Binh commune (95.2 vs 4.8% of hookworm infections).","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of α-thalassemia mutations by Multiplex ligation-dependent probe amplification 通过多重连接依赖性探针扩增检测α地中海贫血突变

Ministry of Science and Technology, Vietnam Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).01-05

T. Le, Vu Viet Ha Vuong, Van Khanh Tran

引用次数: 0