In vitro propagation of Philodendron hederaceum “Lemon Lime”

Thi Thao Ninh, Hong Thuong Nguyen, Truong Son Dinh, Thi Dung Pham, Thi Hue Nong
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Abstract

This study aims to examine the in vitro propagation of Philodendron hederaceum “Lemon Lime”. The results showed that MS medium supplemented with 1.0 mg/l BA was suitable for the rapid multiplication stage, with the rate of shoot proliferation being 2.73 times, the shoots being 5.38 cm in height and the number of leaves/shoot was 4.33 after 6 weeks of culture. In the next stage, in vitro shoots were transferred to MS medium containing 0.25 mg/l IBA for rooting. Within five weeks of culture, 100% of shoots induced roots with a mean of 3.9 roots/plant and the root length was 11.04 cm. In the acclimatisation phase, plantlets achieved 100% ex vitro survival, increased by 2.19 cm in height and 1.96 leaves after four-week acclimatisation in a substrate composed of peatmoss:soil:coconut husk (1:1:1). These research results could be used to cultivate this plant with high proliferation rate and good commercial quality providing seedlings for the floriculture market in Vietnam.
试管繁殖 Philodendron hederaceum "Lemon Lime" (柠檬柠条
本研究旨在考察 "柠檬莱姆"(Philodendron hederaceum "Lemon Lime")的离体繁殖。结果表明,添加 1.0 毫克/升 BA 的 MS 培养基适用于快速繁殖阶段,培养 6 周后,芽的增殖率为 2.73 倍,芽的高度为 5.38 厘米,叶片数/芽为 4.33。下一阶段,将离体芽转移到含 0.25 毫克/升 IBA 的 MS 培养基中进行生根。在 5 周的培养过程中,100% 的芽都生出了根,平均每株生根 3.9 根,根长 11.04 厘米。在适应阶段,在泥炭藓:土壤:椰子壳(1:1:1)组成的基质中适应四周后,小植株的离体存活率达到 100%,高度增加了 2.19 厘米,叶片增加了 1.96 片。这些研究成果可用于培育这种具有高增殖率和良好商业品质的植物,为越南花卉市场提供种苗。
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