Medical mycology最新文献_第7页

Mortality patterns in candidemia: Insights from a multispecies analysis using a global research network. 念珠菌的死亡率模式：使用全球研究网络的多物种分析的见解。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-27 DOI: 10.1093/mmy/myae122

George R Thompson Iii, Daniel B Chastain, Carolina Ferraz, Soubhi Alhayek, Jorge L Salinas, Stefan Sillau, Edward A Stenehjem, Andrés F Henao-Martínez

{"title":"Mortality patterns in candidemia: Insights from a multispecies analysis using a global research network.","authors":"George R Thompson Iii, Daniel B Chastain, Carolina Ferraz, Soubhi Alhayek, Jorge L Salinas, Stefan Sillau, Edward A Stenehjem, Andrés F Henao-Martínez","doi":"10.1093/mmy/myae122","DOIUrl":"10.1093/mmy/myae122","url":null,"abstract":"Understanding the impact of different Candida species on patient outcomes is crucial for effective management and treatment strategies. This study aims to comprehensively analyze the association between Candida species and mortality in documented candidemia. We queried TriNetX, a global research network database, to identify patients diagnosed with candidemia through polymerase chain reaction from 2020 to 2023. The primary outcome was mortality in candidemia patients, categorized by Candida species at 90 days and 1 year. The time to death was assessed using Kaplan-Meier plots. Cox proportional hazard (PH) models were also used for comparative analysis, unadjusted and adjusted for demographic and comorbidity covariates. We captured 1234 candidemia episodes during the study period. The 90-day and 1-year mortality rates for the various Candida species were as follows: C. tropicalis (33.9% and 35.6%), C. glabrata (28.3% and 34%), multispecies (27.7% and 36.4%), C. parapsilosis (25.8% and 31.8%), C. krusei (21.4% and 28.6%), C. albicans (21.1% and 23.9%), and C. auris (13.3% and 15.9%). The unadjusted Kaplan-Meier Survival analysis showed that multispecies candidemia, followed by C. tropicalis, had the lowest survival. The adjusted multivariable Cox PH model found that C.albicans, C. glabrata, C. parapsilosis, C. tropicalis, and multispecies candidemia had significantly higher mortality rates than C. auris. Age and a higher Charlson comorbidity index value emerged as independent predictors of increased mortality. Among patients with candidemia, we found an overall 1-year mortality of 28%. Multispecies candidemia, C. tropicalis, older age, and a higher comorbidity burden were associated with the highest mortality rates.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical characteristics and outcomes of colonization and infection by yeast species in solid organ transplant recipients: Molecular identification and antifungal susceptibility patterns of isolates. 实体器官移植受者中酵母菌定植和感染的临床特征和结果：分离株的分子鉴定和抗真菌敏感性模式。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-27 DOI: 10.1093/mmy/myae118

Somayeh Yazdanpanah, Mojtaba Shafiekhani, Mohammad Ahmadi, Zahra Zare, Hamed Nikoupour, Sara Arabsheybani, Bita Geramizadeh, Mohammad-Hossein Anbardar, Parisa Chamanpara, Hamid Badali, Mohsen Moghadami, Keyvan Pakshir, Kamiar Zomorodian

{"title":"Clinical characteristics and outcomes of colonization and infection by yeast species in solid organ transplant recipients: Molecular identification and antifungal susceptibility patterns of isolates.","authors":"Somayeh Yazdanpanah, Mojtaba Shafiekhani, Mohammad Ahmadi, Zahra Zare, Hamed Nikoupour, Sara Arabsheybani, Bita Geramizadeh, Mohammad-Hossein Anbardar, Parisa Chamanpara, Hamid Badali, Mohsen Moghadami, Keyvan Pakshir, Kamiar Zomorodian","doi":"10.1093/mmy/myae118","DOIUrl":"10.1093/mmy/myae118","url":null,"abstract":"Fungal infections are serious complications after solid organ transplantation, with high mortality and morbidity. Given the importance of the local epidemiological data and also extensive prophylactic regimens in solid organ transplant (SOT) recipients, this study aimed to investigate the clinical characteristics and resistance patterns of yeast isolates in SOT recipients at a main referral transplant center in Iran. Of the 275 recipients enrolled, 22 (8%) had at least one positive yeast culture at a median of 5 days after transplantation. Bacterial infection and reoperation were significantly associated with colonization or infection caused by yeast species (P = .001). Moreover, mortality and length of ICU/hospital stay were significantly higher in patients with positive yeast cultures (P < .05). The most frequent species isolated was Candida albicans (50%), followed by C. glabrata (22.7%). Of species with definite breakpoints, the fluconazole-resistant rate was 23%. Caspofungin and amphotericin B showed potent activity against all isolates. Regarding the high risk of fungal infections, awareness of local epidemiological trends and resistance patterns can help improve outcomes in SOT recipients.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new quantitative reverse transcription PCR assay to improve the routine diagnosis of paracoccidioidomycosis. 一种新的定量反转录PCR方法提高副球孢子菌病的常规诊断。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-27 DOI: 10.1093/mmy/myae125

Priscila Marques de Macedo, Aude Sturny-Leclère, Samia Hamane, Thierry Pautet, Anderson Messias Rodrigues, Dayvison Francis Saraiva Freitas, Antonio Carlos Francesconi do Valle, Rosely Maria Zancopé-Oliveira, Rodrigo Almeida-Paes, Alexandre Alanio

{"title":"A new quantitative reverse transcription PCR assay to improve the routine diagnosis of paracoccidioidomycosis.","authors":"Priscila Marques de Macedo, Aude Sturny-Leclère, Samia Hamane, Thierry Pautet, Anderson Messias Rodrigues, Dayvison Francis Saraiva Freitas, Antonio Carlos Francesconi do Valle, Rosely Maria Zancopé-Oliveira, Rodrigo Almeida-Paes, Alexandre Alanio","doi":"10.1093/mmy/myae125","DOIUrl":"10.1093/mmy/myae125","url":null,"abstract":"Paracoccidioides are dimorphic fungal pathogens and the etiological agents of paracoccidioidomycosis (PCM). This severe systemic mycosis is restricted to Latin America, where it has been historically endemic. Currently, PCM presents the fewest diagnostic tools available when compared to other endemic mycoses. The main PCM diagnostic methods also have limitations. Molecular methods using different protocols have been proposed, but are restricted to a few regions. An analytical transversal study was conducted to evaluate a new molecular tool using specimens from patients diagnosed with PCM at a reference center for endemic mycoses in Rio de Janeiro, Brazil. After whole nucleic acid (WNA) extraction, RT-qPCR was performed in two independent simplex reactions, targeting the mitochondrial small subunit ribosomal RNA genes of Paracoccidioides brasiliensis and Paracoccidioides lutzii. Additionally, WNAs from all PCM-related Paracoccidioides species and from 114 other fungal strains, as well as from samples obtained from patients diagnosed with other endemic mycoses and tuberculosis, were also tested for specificity. The RT-qPCR targeting P. brasiliensis successfully amplified genetic material from all tested Paracoccidioides species but not P. lutzii, which is why a specific RT-qPCR was designed. The RT-qPCR efficiency was 1.95 (95%) with 100% analytical specificity for both targets. All included PCM clinical samples were positive (100% sensitivity) for P. brasiliensis, and all yielded negative for P. lutzii. Additionally, all samples collected from patients with other diseases were negative, reinforcing the assay's specificity. In conclusion, this study proposes a new accurate tool to cover gaps, contributing to the molecular diagnosis of this neglected disease.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malassezia yeast population dynamics on the skin of patients living with HIV. 艾滋病毒感染者皮肤上马拉色菌酵母菌群的动态变化。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-27 DOI: 10.1093/mmy/myae120

Abdourahim Abdillah, Isabelle Ravaux, Saadia Mokhtari, Stéphane Ranque

{"title":"Malassezia yeast population dynamics on the skin of patients living with HIV.","authors":"Abdourahim Abdillah, Isabelle Ravaux, Saadia Mokhtari, Stéphane Ranque","doi":"10.1093/mmy/myae120","DOIUrl":"10.1093/mmy/myae120","url":null,"abstract":"Malassezia species are lipid-dependent yeasts of the normal skin mycobiota in humans and some animals. Yet, both the dynamic of Malassezia skin colonization and the associated fungal and bacterial skin microbiome remain unknown in HIV+ patients. This study aimed to compare Malassezia yeast community structure and associated microbiome on the healthy skin of HIV+ patients and healthy controls. A total of 23 HIV+ patients and 10 healthy controls were included and followed-up for a maximum of 5 visits over 10-17 months. At each visit, chest, face, nasolabial fold, and scalp skin samples were subjected to both culture and MALDI-TOF MS identification, and ITS/16S metabarcoding. The participants were categorized according to their Malassezia colony forming unit (CFU) abundance. Malassezia were cultured from each participant at each visit. HIV+ patients were highly colonized on all visits with CFU > 100. Malassezia sympodialis and M. globosa were the most dominant species. Malassezia furfur and M. dermatis were more prevalent in HIV+ than in healthy participants. Malassezia sympodialis prevalence was stable at each sampling sites over time. Malassezia furfur prevalence was stable and more abundant over time on HIV+ patients' chest. The metabarcoding analysis suggested a higher fungal and bacterial diversity and an increased abundance of Cladosporium halotolerans and Streptococcus in HIV+ patients than in controls. Overall, HIV+ patients display a high skin colonization by Malassezia yeasts and a dysbiosis of both fungal and bacterial communities.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptococcus neoformans/gattii and Histoplasma capsulatum var. capsulatum infections on tissue sections: Diagnostic pitfalls and relevance of an integrated histomolecular diagnosis. 组织切片上的新型隐球菌/ gatii和荚膜组织胞浆变异感染：诊断缺陷和综合组织分子诊断的相关性。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-27 DOI: 10.1093/mmy/myae126

Alexis Trecourt, Meja Rabodonirina, Marie Donzel, Emmanuelle Chapey-Picq, Abderrazzak Bentaher, Damien Dupont, Charline Miossec, Florence Persat, Martine Wallon, Jean-Philippe Lemoine, Pauline Tirard-Collet, Aline Baltrès, Alexandre Alanio, Mojgan Devouassoux-Shisheboran, Jean Menotti

{"title":"Cryptococcus neoformans/gattii and Histoplasma capsulatum var. capsulatum infections on tissue sections: Diagnostic pitfalls and relevance of an integrated histomolecular diagnosis.","authors":"Alexis Trecourt, Meja Rabodonirina, Marie Donzel, Emmanuelle Chapey-Picq, Abderrazzak Bentaher, Damien Dupont, Charline Miossec, Florence Persat, Martine Wallon, Jean-Philippe Lemoine, Pauline Tirard-Collet, Aline Baltrès, Alexandre Alanio, Mojgan Devouassoux-Shisheboran, Jean Menotti","doi":"10.1093/mmy/myae126","DOIUrl":"10.1093/mmy/myae126","url":null,"abstract":"Cryptococcus neoformans/gattii and Histoplasma capsulatum var. capsulatum may present atypical histopathological features inducing diagnostic errors. We aimed to estimate the frequency of these atypical features in formalin-fixed tissue (FT) samples and to assess the relevance of an integrated histomolecular diagnosis using specific H. capsulatum PCR and panfungal PCR followed by Sanger sequencing and/or targeted massive parallel sequencing (MPS). A total of 27 FT from 23 patients with a histopathological diagnosis of cryptococcosis (n = 16 FT from 13 patients) or histoplasmosis (n = 11 FT from 10 patients) were retrospectively included. All FT were consultation cases. Mycological identifications on equivalent fresh tissue were available for 11/23 (47.8%) patients. The expert pathologist review modified the diagnosis suggested by the initial pathologist in 7/27 (25.9%) FT. Fungal morphology and tissue inflammation were compared between both mycoses. The most discriminant atypical criterion was the presence of dented-looking yeasts, observed in 68.75% (11/16) of C. neoformans/gattii and none (0/11) of H. capsulatum var. capsulatum (P = .002). For the 12/23 (52.2%) patients without mycological identification on fresh tissue, an integrated histomolecular diagnosis on FT using specific PCR or panfungal PCR followed by Sanger sequencing and/or MPS led to fungal identification in 9/12 (75%) cases; for cryptococcosis, the targeted MPS sensitivity was higher than that of Sanger sequencing (P = .041). Thus, because atypical histopathological features may be tricky, integrated histomolecular diagnosis is essential for optimal patient care.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical utility of pharmacogenomic testing for patients with coccidioidal meningitis. 球虫脑膜炎患者药物基因组学检测的临床实用性。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-05 DOI: 10.1093/mmy/myae113

Rawan Elkurdi, Marie F Grill, Adrijana Kekic, Janis E Blair

引用次数: 0

Pulmonary aspergillosis in green sea turtles (Chelonia mydas): A case series. 绿海龟（Chelonia mydas）肺曲霉菌病：绿海龟曲霉菌病病例系列。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-05 DOI: 10.1093/mmy/myae114

Vanice Rodrigues Poester, Jessica E Dávila Hidalgo, Lívia Silveira Munhoz, Mariana Rodrigues Trápaga, Emília Ferreira Andrade, Paula Lima Canabarro, Pamela Parmigiani, Eduarda Corrêa Freitas, Mariana Martins Flores, Éryca Ceolin Lamego, Andréa Corrado Adornes, Alessandro Comarú Pasqualotto, David A Stevens, Melissa Orzechowski Xavier

引用次数: 0

Association between preoperative antifungal therapy and postoperative complications in patients with pulmonary aspergilloma: A national database study in Japan. 肺曲菌瘤患者术前抗真菌治疗与术后并发症的关系：日本国家数据库研究

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-05 DOI: 10.1093/mmy/myae117

Keita Takeda, Akira Yokoyama, Takeshi Fukami, Yuya Kimura, Maho Suzukawa, Taisuke Jo, Junko Suzuki, Yuka Sasaki, Akihisa Mitani, Goh Tanaka, Asahi Fujita, Hiroki Matsui, Kiyohide Fushimi, Takahide Nagase, Hideo Yasunaga

{"title":"Association between preoperative antifungal therapy and postoperative complications in patients with pulmonary aspergilloma: A national database study in Japan.","authors":"Keita Takeda, Akira Yokoyama, Takeshi Fukami, Yuya Kimura, Maho Suzukawa, Taisuke Jo, Junko Suzuki, Yuka Sasaki, Akihisa Mitani, Goh Tanaka, Asahi Fujita, Hiroki Matsui, Kiyohide Fushimi, Takahide Nagase, Hideo Yasunaga","doi":"10.1093/mmy/myae117","DOIUrl":"10.1093/mmy/myae117","url":null,"abstract":"Postoperative complications of pulmonary resection in patients with pulmonary aspergilloma are common. Preoperative antifungal therapy is a perioperative management strategy to reduce postoperative complications; however, the benefit of the treatment remains controversial. A retrospective cohort study was conducted using data from the Diagnosis Procedure Combination database (July 2010-March 2021), a national inpatient database in Japan. Patients with pulmonary aspergilloma with or without chronic necrotizing pulmonary aspergillosis who underwent pulmonary resection were identified and divided into two groups based on whether they received preoperative antifungal therapy. Propensity-score overlap weighting was performed to compare the occurrence of composite postoperative complications and postoperative length of hospital stay between the two groups. We identified 98 patients with pulmonary aspergilloma who received preoperative antifungal therapy and 399 patients who did not. After propensity-score overlap weighting, the preoperative antifungal therapy group had a significantly lower proportion of composite postoperative complications than the non-preoperative antifungal therapy group (6.8% vs. 14.2%, P = .038). There was no significant difference in postoperative length of stay between the groups with and without preoperative antifungal therapy (median 14.6 vs. 15.8 days, P = .41). Preoperative antifungal therapy was associated with a reduction in postoperative complications in patients with pulmonary aspergilloma. Preoperative treatment with antifungals may be beneficial in reducing the risk of postoperative complications in patients with pulmonary aspergilloma undergoing pulmonary resection.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antemortem diagnostic tests for the detection of Aspergillus infection in birds: A systematic review. 用于检测鸟类曲霉菌感染的尸检诊断测试：系统综述。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae112

Sabrina Vieu, Jacques Guillot, François Beaudeau

{"title":"Antemortem diagnostic tests for the detection of Aspergillus infection in birds: A systematic review.","authors":"Sabrina Vieu, Jacques Guillot, François Beaudeau","doi":"10.1093/mmy/myae112","DOIUrl":"10.1093/mmy/myae112","url":null,"abstract":"Aspergillosis remains a common and life-threatening disease in captive and wild birds all over the world. The diagnosis is currently based on clinical signs or lesions, diagnostic imaging, and a variety of biological tests. This systematic review aimed to compare the accuracy of antemortem diagnostic tests for Aspergillus infection in birds. According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a search was conducted on PubMed, Scopus, Web of Science, and CAB until January 2024. The methodological quality was assessed with QUADAS 2 risk of bias tool. The 13 studies, selected for the review, included results from a wide variety of birds (mainly Spheniciformes but also Falconiformes, Psittaciformes, and Galliformes) from wildlife rehabilitation centers, zoological parks, or veterinary practices. Aspergillus infection was mainly confirmed by fungal culture and/or histopathology. Serum markers included Aspergillus components (galactomannan, β-d-glucan, mannoproteins, and gliotoxin), anti-Aspergillus antibodies, 3-hydroxybutyrate, as well as protein electrophoresis and acute phase molecules. Sensitivity and specificity displayed a large amount of variation despite threshold arrangement. Disparities in the number of individuals per study did not allow for reliable comparison. Platelia Ag Assay (Bio-Rad), the most commonly used test in the studies, demonstrated moderate specificity and low sensitivity. Overall, non-specific tests demonstrated more consistent performance, whereas specific tests showed greater variability. Based on current knowledge, none of these tests provide sufficient accuracy to reliably detect Aspergillus infection in birds in clinical practice.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geraniol inhibits both planktonic cells and biofilms of the Candida parapsilosis species complex: Highlight for the improved efficacy of amphotericin B, caspofungin and fluconazole plus Geraniol. 香叶醇可抑制副丝状念珠菌菌种群的浮游细胞和生物膜：两性霉素 B、卡泊芬净和氟康唑加香叶醇可提高疗效的亮点。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae105

José Júlio Costa Sidrim, Daniel Vieira Martins, Maria Gleiciane da Rocha, Géssica Dos Santos Araújo, Rossana de Aguiar Cordeiro, Glaucia Morgana de Melo Guedes, Waldemiro de Aquino Pereira-Neto, Débora de Souza Collares Maia Castelo-Branco, Marcos Fábio Gadelha Rocha

{"title":"Geraniol inhibits both planktonic cells and biofilms of the Candida parapsilosis species complex: Highlight for the improved efficacy of amphotericin B, caspofungin and fluconazole plus Geraniol.","authors":"José Júlio Costa Sidrim, Daniel Vieira Martins, Maria Gleiciane da Rocha, Géssica Dos Santos Araújo, Rossana de Aguiar Cordeiro, Glaucia Morgana de Melo Guedes, Waldemiro de Aquino Pereira-Neto, Débora de Souza Collares Maia Castelo-Branco, Marcos Fábio Gadelha Rocha","doi":"10.1093/mmy/myae105","DOIUrl":"10.1093/mmy/myae105","url":null,"abstract":"The Candida parapsilosis species complex poses a recognized threat to the nosocomial environment. In the scenario of the global rise of resistant strains to antifungals, geraniol, a terpene isolated from different essential oils, has shown promising antimicrobial activity. We evaluated: (1) the effects of geraniol against the C. parapsilosis species complex, in planktonic and biofilm forms; (2) the strains' susceptibility to clinical antifungals and (3) the geraniol interaction with antifungals. Eighteen isolates were subjected to in vitro susceptibility testing by the broth microdilution protocol, using geraniol, amphotericin B, caspofungin, itraconazole and fluconazole to determine the minimum inhibitory concentration (MIC) and subsequently, we measured the fungicidal activity. Geraniol was tested against biofilms by the measurement of the metabolic activity and biomass. Pharmacological interactions were performed by the checkerboard method. Geraniol's MIC range was between 256 and 512 µg/ml. MIC range for clinical antifungals was ≤ 0.031-4 µg/ml. Geraniol also showed antibiofilm activity with average reductions of metabolic activity (38.33%) and biomass (30.69%), at MIC concentration. Furthermore, geraniol showed synergistic/additive effects with antifungals. Briefly, geraniol inhibits both planktonic cells and biofilms of the C. parapsilosis species complex and besides it improves the efficacy of amphotericin B, caspofungin and fluconazole.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0