MicrobiologyOpen最新文献_第9页

Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae 副猪绿脓杆菌、缩耳支原体和滑膜支原体多重定量PCR检测方法的建立

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-05-15 DOI: 10.1002/mbo3.1353

Simone Scherrer, Sarah Schmitt, Fenja Rademacher, Peter Kuhnert, Giovanni Ghielmetti, Sophie Peterhans, Roger Stephan

{"title":"Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae","authors":"Simone Scherrer, Sarah Schmitt, Fenja Rademacher, Peter Kuhnert, Giovanni Ghielmetti, Sophie Peterhans, Roger Stephan","doi":"10.1002/mbo3.1353","DOIUrl":"10.1002/mbo3.1353","url":null,"abstract":"Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae are important porcine pathogens responsible for polyserositis, polyarthritis, meningitis, pneumonia, and septicemia causing significant economic losses in the swine industry. A new multiplex quantitative polymerase chain reaction (qPCR) was designed on one hand for the detection of G. parasuis and the virulence marker vtaA to distinguish between highly virulent and non-virulent strains. On the other hand, fluorescent probes were established for the detection and identification of both M. hyorhinis and M. hyosynoviae targeting 16S ribosomal RNA genes. The development of the qPCR was based on reference strains of 15 known serovars of G. parasuis, as well as on the type strains M. hyorhinis ATCC 17981T and M. hyosynoviae NCTC 10167T. The new qPCR was further evaluated using 21 G. parasuis, 26 M. hyorhinis, and 3 M. hyosynoviae field isolates. Moreover, a pilot study including different clinical specimens of 42 diseased pigs was performed. The specificity of the assay was 100% without cross-reactivity or detection of other bacterial swine pathogens. The sensitivity of the new qPCR was demonstrated to be between 11–180 genome equivalents (GE) of DNA for M. hyosynoviae and M. hyorhinis, and 140–1200 GE for G. parasuis and vtaA. The cut-off threshold cycle was found to be at 35. The developed sensitive and specific qPCR assay has the potential to become a useful molecular tool, which could be implemented in veterinary diagnostic laboratories for the detection and identification of G. parasuis, its virulence marker vtaA, M. hyorhinis, and M. hyosynoviae.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 3","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1353","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9726449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes 鱼腥鱼PCC7120对林丹的响应:林丹潜在基因的生理效应和差异表达

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-04-27 DOI: 10.1002/mbo3.1355

Jorge Guío, Maria F. Fillat, Maria L. Peleato, Emma Sevilla

{"title":"Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes","authors":"Jorge Guío, Maria F. Fillat, Maria L. Peleato, Emma Sevilla","doi":"10.1002/mbo3.1355","DOIUrl":"10.1002/mbo3.1355","url":null,"abstract":"Lindane (γ-HCH) is an organochlorine pesticide that causes huge environmental concerns worldwide due to its recalcitrance and toxicity. The use of the cyanobacterium Anabaena sp. PCC 7120 in aquatic lindane bioremediation has been suggested but information relative to this process is scarce. In the present work, data relative to the growth, pigment composition, photosynthetic/respiration rate, and oxidative stress response of Anabaena sp. PCC 7120 in the presence of lindane at its solubility limit in water are shown. In addition, lindane degradation experiments revealed almost a total disappearance of lindane in the supernatants of Anabaena sp. PCC 7120 culture after 6 days of incubation. The diminishing in lindane concentration was in concordance with an increase in the levels of trichlorobenzene inside the cells. Furthermore, to identify potential orthologs of the linA, linB, linC, linD, linE, and linR genes from Sphingomonas paucimobilis B90A in Anabaena sp. PCC 7120, a whole genome screening was performed allowing the identification of five putative lin orthologs (all1353 and all0193 putative orthologs of linB, all3836 putative orthologs of linC, and all0352 and alr0353 putative orthologs of linE and linR, respectively) which could be involved in the lindane degradation pathway. Differential expression analysis of these genes in the presence of lindane revealed strong upregulation of one of the potential lin genes of Anabaena sp. PCC 7120.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 3","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9726451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of sponge-to-sponge contact on the microbiomes of three spatially competing Caribbean coral reef species 海绵对海绵接触对三种空间竞争的加勒比海珊瑚礁物种微生物组的影响

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-04-27 DOI: 10.1002/mbo3.1354

Shelby E. Gantt, Patrick M. Erwin

{"title":"Effects of sponge-to-sponge contact on the microbiomes of three spatially competing Caribbean coral reef species","authors":"Shelby E. Gantt, Patrick M. Erwin","doi":"10.1002/mbo3.1354","DOIUrl":"10.1002/mbo3.1354","url":null,"abstract":"Sponges perform important ecosystem functions, host diverse microbial symbiont communities (microbiomes), and have been increasing in density on Caribbean coral reefs over the last decade. Sponges compete for space in coral reef communities through both morphological and allelopathic strategies, but no studies of microbiome impacts during these interactions have been conducted. Microbiome alterations mediate spatial competition in other coral reef invertebrates and may similarly impact competitive outcomes for sponges. In this study, we characterized the microbiomes of three common Caribbean sponges (Agelas tubulata, Iotrochota birotulata, and Xestospongia muta) observed to naturally interact spatially in Key Largo, Florida (USA). For each species, replicate samples were collected from sponges in contact with neighbors at the site of contact (contact) and distant from the site of contact (no contact), and from sponges spatially isolated from neighbors (control). Next-generation amplicon sequencing (V4 region of 16S rRNA) revealed significant differences in microbial community structure and diversity among sponge species, but no significant effects were observed within sponge species across all contact states and competitor pairings, indicating no large community shifts in response to direct contact. At a finer scale, particular symbiont taxa (operational taxonomic units at 97% sequence identity, OTUs) were shown to decrease significantly in some interaction pairings, suggesting localized effects for specific sponge competitors. Overall, these results revealed that direct contact during spatial competition does not significantly alter microbial community composition or structure of interacting sponges, suggesting that allelopathic interactions and competitive outcomes are not mediated by microbiome damage or destabilization.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 3","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9731860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomics of the “tumorigenes” clade of the family Rhizobiaceae and description of Rhizobium rhododendri sp. nov. 根瘤菌科“致瘤性”分支的基因组学研究及杜鹃花根瘤菌的描述。

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-31 DOI: 10.1002/mbo3.1352

Nemanja Kuzmanović, George C. diCenzo, Boyke Bunk, Cathrin Spröer, Anja Frühling, Meina Neumann-Schaal, Jörg Overmann, Kornelia Smalla

{"title":"Genomics of the “tumorigenes” clade of the family Rhizobiaceae and description of Rhizobium rhododendri sp. nov.","authors":"Nemanja Kuzmanović, George C. diCenzo, Boyke Bunk, Cathrin Spröer, Anja Frühling, Meina Neumann-Schaal, Jörg Overmann, Kornelia Smalla","doi":"10.1002/mbo3.1352","DOIUrl":"10.1002/mbo3.1352","url":null,"abstract":"Tumorigenic members of the family Rhizobiaceae, known as agrobacteria, are responsible for crown and cane gall diseases of various crops worldwide. Tumorigenic agrobacteria are commonly found in the genera Agrobacterium, Allorhizobium, and Rhizobium. In this study, we analyzed a distinct “tumorigenes” clade of the genus Rhizobium, which includes the tumorigenic species Rhizobium tumorigenes, as well as strains causing crown gall disease on rhododendron. Here, high-quality, closed genomes of representatives of the “tumorigenes” clade were generated, followed by comparative genomic and phylogenomic analyses. Additionally, the phenotypic characteristics of representatives of the “tumorigenes” clade were analyzed. Our results showed that the tumorigenic strains isolated from rhododendron represent a novel species of the genus Rhizobium for which the name Rhizobium rhododendri sp. nov. is proposed. This species also includes additional strains originating from blueberry and Himalayan blackberry in the United States, whose genome sequences were retrieved from GenBank. Both R. tumorigenes and R. rhododendri contain multipartite genomes, including a chromosome, putative chromids, and megaplasmids. Synteny and phylogenetic analyses indicated that a large putative chromid of R. rhododendri resulted from the cointegration of an ancestral megaplasmid and two putative chromids, following its divergence from R. tumorigenes. Moreover, gene clusters specific for both species of the “tumorigenes” clade were identified, and their biological functions and roles in the ecological diversification of R. rhododendri and R. tumorigenes were predicted and discussed.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1352","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9475355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

High prevalence of blaCTX-M-15 type extended-spectrum beta-lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction 冈比亚秃鹫中blaCTX-M-15型超广谱β-内酰胺酶的高流行率：一种具有大量人类相互作用的濒危物种

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-23 DOI: 10.1002/mbo3.1349

Hanna Woksepp, Fagimba Camara, Jonas Bonnedahl

{"title":"High prevalence of blaCTX-M-15 type extended-spectrum beta-lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction","authors":"Hanna Woksepp, Fagimba Camara, Jonas Bonnedahl","doi":"10.1002/mbo3.1349","DOIUrl":"https://doi.org/10.1002/mbo3.1349","url":null,"abstract":"One hundred fecal samples from hooded vultures in the Gambia (Banjul area) were investigated for the presence of bacteria with extended-spectrum cephalosporin- (ESBL/AmpC), carbapenemases, and colistin resistance. No Enterobacteriales carrying carbapenemases or resistance against colistin were detected. Fifty-four ESBL-producing Escherichia coli and five ESBL-producing Klebsiella pneumoniae isolates were identified in 52 of the samples, of which 52 E. coli and 4 K. pneumoniae yielded passed sequencing results. Fifty of the E. coli had ESBL phenotype and genotype harboring blaCTX-M genes, of which 88.5% (n = 46) were the blaCTX-M-15 gene, commonly found on the African continent. Furthermore, the genetic context around blaCTX-M-15 was similar between isolates, being colocalized with ISKpn19. In contrast, cgMLST analysis of the E. coli harboring ESBL genes revealed a genetic distribution over a large fraction of the currently known existing E. coli populations in the Gambia. Hooded vultures in the Gambia thus have a high ESBL E. coli-prevalence (>50%) with low diversity regarding key resistance genes. Furthermore, given the urban presence and frequent interactions between hooded vultures and humans, data from this study implies hooded vultures as potential vectors contributing to the further dissemination of antibiotic-resistance genes.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1349","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50142216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Biological and synthetic surfactant exposure increases antimicrobial gene occurrence in a freshwater mixed microbial biofilm environment 生物和合成表面活性剂暴露增加了淡水混合微生物生物膜环境中抗菌基因的发生

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-17 DOI: 10.1002/mbo3.1351

Stephanie P. Gill, William J. Snelling, James S. G. Dooley, Nigel G. Ternan, Ibrahim M. Banat, Joerg Arnscheidt, William R. Hunter

{"title":"Biological and synthetic surfactant exposure increases antimicrobial gene occurrence in a freshwater mixed microbial biofilm environment","authors":"Stephanie P. Gill, William J. Snelling, James S. G. Dooley, Nigel G. Ternan, Ibrahim M. Banat, Joerg Arnscheidt, William R. Hunter","doi":"10.1002/mbo3.1351","DOIUrl":"10.1002/mbo3.1351","url":null,"abstract":"Aquatic habitats are particularly susceptible to chemical pollution, such as antimicrobials, from domestic, agricultural, and industrial sources. This has led to the rapid increase of antimicrobial resistance (AMR) gene prevalence. Alternate approaches to counteract pathogenic bacteria are in development including synthetic and biological surfactants such as sodium dodecyl sulfate (SDS) and rhamnolipids. In the aquatic environment, these surfactants may be present as pollutants with the potential to affect biofilm formation and AMR gene occurrence. We tested the effects of rhamnolipid and SDS on aquatic biofilms in a freshwater stream in Northern Ireland. We grew biofilms on contaminant exposure substrates deployed within the stream over 4 weeks. We then extracted DNA and carried out shotgun sequencing using a MinION portable sequencer to determine microbial community composition, with 16S rRNA analyses (64,678 classifiable reads identified), and AMR gene occurrence (81 instances of AMR genes over 9 AMR gene classes) through a metagenomic analysis. There were no significant changes in community composition within all systems; however, biofilm exposed to rhamnolipid had a greater number of unique taxa as compared to SDS treatments and controls. AMR gene prevalence was higher in surfactant-treated biofilms, although not significant, with biofilm exposed to rhamnolipids having the highest presence of AMR genes and classes compared to the control or SDS treatments. Our results suggest that the presence of rhamnolipid encourages an increase in the prevalence of AMR genes in biofilms produced in mixed-use water bodies.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9475357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater 处理过的废水中高微生物多样性对模型溪流生态系统的时间干扰

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-14 DOI: 10.1002/mbo3.1347

Tom L. Stach, Guido Sieber, Manan Shah, Sophie A. Simon, André Soares, Till L. V. Bornemann, Julia Plewka, Julian Künkel, Christian Becker, Folker Meyer, Jens Boenigk, Alexander J. Probst

{"title":"Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater","authors":"Tom L. Stach, Guido Sieber, Manan Shah, Sophie A. Simon, André Soares, Till L. V. Bornemann, Julia Plewka, Julian Künkel, Christian Becker, Folker Meyer, Jens Boenigk, Alexander J. Probst","doi":"10.1002/mbo3.1347","DOIUrl":"10.1002/mbo3.1347","url":null,"abstract":"Microbial communities in freshwater streams play an essential role in ecosystem functioning via biogeochemical cycling. Yet, the impacts of treated wastewater influx into stream ecosystems on microbial strain diversity remain mostly unexplored. Here, we coupled full-length 16S ribosomal RNA gene Nanopore sequencing and strain-resolved metagenomics to investigate the impact of treated wastewater on a mesocosm system (AquaFlow) run with restored river water. Over 10 days, community Bray–Curtis dissimilarities between treated and control mesocosm decreased (0.57 ± 0.058 to 0.26 ± 0.046) based on ribosomal protein S3 gene clustering, finally converging to nearly identical communities. Similarly, strain-resolved metagenomics revealed a high diversity of bacteria and viruses after the introduction of treated wastewater; these microbes also decreased over time resulting in the same strain clusters in control and treatment at the end of the experiment. Specifically, 39.2% of viral strains detected in all samples were present after the introduction of treated wastewater only. Although bacteria present at low abundance in the treated wastewater introduced additional antibiotic resistance genes, signals of naturally occurring ARG-encoding organisms resembled the resistome at the endpoint. Our results suggest that the previously stressed freshwater stream and its microbial community are resilient to a substantial introduction of treated wastewater.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1347","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9846259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A parasitic nematode induces dysbiosis in susceptible but not resistant gastropod hosts 寄生线虫在易感但不耐药的腹足类宿主中诱导生态失调

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-10 DOI: 10.1002/mbo3.1346

Laura Sheehy, Kerry MacDonald-Howard, Chris D. Williams, Gareth D. Weedall, Hayley Jones, Robbie Rae

{"title":"A parasitic nematode induces dysbiosis in susceptible but not resistant gastropod hosts","authors":"Laura Sheehy, Kerry MacDonald-Howard, Chris D. Williams, Gareth D. Weedall, Hayley Jones, Robbie Rae","doi":"10.1002/mbo3.1346","DOIUrl":"10.1002/mbo3.1346","url":null,"abstract":"Animals’ gut microbiomes affect a wide array of biological processes including immunity and protection from pathogens. However, how the microbiome changes due to infection by parasites is still largely unknown, as is how the microbiome changes in hosts that differ in their susceptibility to parasites. To investigate this, we exposed two slug species of differing susceptibility to the parasitic nematode Phasmarhabditis hermaphrodita (Deroceras reticulatum is highly susceptible and Ambigolimax valentianus resistant to the nematode) and profiled the gut microbiota after 7 and 14 days. Before infection, both slug species’ microbiota was dominated by similar bacterial genera: Pseudomonas (by far the most abundant), Sphingobacterium, Pedobacter, Chryseobacterium, and Flavobacterium. In the resistant host A. valentianus, there was no significant change in the bacterial genera after infection, but in D. reticulatum, the bacterial profile changed, with a decrease in the abundance of Pseudomonadaceae and an increase in the abundance of Flavobacteriaceae and Sphingobacteriaceae after 7 days postinfection. This suggests nematode infection causes dysbiosis in hosts that are susceptible to infection, but the microbiome of resistant species remains unaltered. In summary, the regulation of the immune system is tightly linked with host survival, and nematode infection can alter the microbiome structure.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9846264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Highly efficient export of a disulfide-bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli 在大肠杆菌中使用CyDisCo通过Tat途径高效地将二硫结合蛋白输出到外质和培养基中

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-08 DOI: 10.1002/mbo3.1350

Klaudia Arauzo-Aguilera, Mirva J. Saaranen, Colin Robinson, Lloyd W. Ruddock

{"title":"Highly efficient export of a disulfide-bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli","authors":"Klaudia Arauzo-Aguilera, Mirva J. Saaranen, Colin Robinson, Lloyd W. Ruddock","doi":"10.1002/mbo3.1350","DOIUrl":"10.1002/mbo3.1350","url":null,"abstract":"High-value heterologous proteins produced in Escherichia coli that contain disulfide bonds are almost invariably targeted to the periplasm via the Sec pathway as it, among other advantages, enables disulfide bond formation and simplifies downstream processing. However, the Sec system cannot transport complex or rapidly folding proteins, as it only transports proteins in an unfolded state. The Tat system also transports proteins to the periplasm, and it has significant potential as an alternative means of recombinant protein production because it transports fully folded proteins. Most of the studies related to Tat secretion have used the well-studied TorA signal peptide that is Tat-specific, but this signal peptide also tends to induce degradation of the protein of interest, resulting in lower yields. This makes it difficult to use Tat in the industry. In this study, we show that a model disulfide bond-containing protein, YebF, can be exported to the periplasm and media at a very high level by the Tat pathway in a manner almost completely dependent on cytoplasmic disulfide formation, by other two putative Tat SPs: those of MdoD and AmiC. In contrast, the TorA SP exports YebF at a low level.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9472333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The abundance of the potential pathogen Staphylococcus hominis in the air microbiome in a dental clinic and its susceptibility to far-UVC light 牙科诊所空气微生物群中潜在病原体人型葡萄球菌的丰度及其对远紫外线光的敏感性

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-05 DOI: 10.1002/mbo3.1348

Marilena Aquino de Muro, Igor Shuryak, Anne-Catrin Uhlemann, Alice Tillman, Dwayne Seeram, Joseph Zakaria, David Welch, Steven M. Erde, David J. Brenner

{"title":"The abundance of the potential pathogen Staphylococcus hominis in the air microbiome in a dental clinic and its susceptibility to far-UVC light","authors":"Marilena Aquino de Muro, Igor Shuryak, Anne-Catrin Uhlemann, Alice Tillman, Dwayne Seeram, Joseph Zakaria, David Welch, Steven M. Erde, David J. Brenner","doi":"10.1002/mbo3.1348","DOIUrl":"10.1002/mbo3.1348","url":null,"abstract":"The dental clinic air microbiome incorporates microbes from the oral cavity and upper respiratory tract (URT). This study aimed to establish a reliable methodology for air sampling in a dental clinic setting and quantify the abundance of culturable mesophilic aerobic bacteria present in these samples using regression modeling. Staphylococcus hominis, a potentially pathogenic bacterium typically found in the human oropharynx and URT, was consistently isolated. S. hominis was the most abundant species of aerobic bacteria (22%–24%) and comprised 60%–80% of all Staphylococcus spp. The study also assessed the susceptibility of S. hominis to 222 nm-far-UVC light in laboratory experiments, which showed an exponential surface inactivation constant of k = 0.475 cm2/mJ. This constant is a critical parameter for future on-site use of far-UVC light as a technique for reducing pathogenic bacterial load in dental clinics.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9846263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0