MicrobiologyOpen最新文献_第9页

Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater 处理过的废水中高微生物多样性对模型溪流生态系统的时间干扰

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-14 DOI: 10.1002/mbo3.1347

Tom L. Stach, Guido Sieber, Manan Shah, Sophie A. Simon, André Soares, Till L. V. Bornemann, Julia Plewka, Julian Künkel, Christian Becker, Folker Meyer, Jens Boenigk, Alexander J. Probst

{"title":"Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater","authors":"Tom L. Stach, Guido Sieber, Manan Shah, Sophie A. Simon, André Soares, Till L. V. Bornemann, Julia Plewka, Julian Künkel, Christian Becker, Folker Meyer, Jens Boenigk, Alexander J. Probst","doi":"10.1002/mbo3.1347","DOIUrl":"10.1002/mbo3.1347","url":null,"abstract":"Microbial communities in freshwater streams play an essential role in ecosystem functioning via biogeochemical cycling. Yet, the impacts of treated wastewater influx into stream ecosystems on microbial strain diversity remain mostly unexplored. Here, we coupled full-length 16S ribosomal RNA gene Nanopore sequencing and strain-resolved metagenomics to investigate the impact of treated wastewater on a mesocosm system (AquaFlow) run with restored river water. Over 10 days, community Bray–Curtis dissimilarities between treated and control mesocosm decreased (0.57 ± 0.058 to 0.26 ± 0.046) based on ribosomal protein S3 gene clustering, finally converging to nearly identical communities. Similarly, strain-resolved metagenomics revealed a high diversity of bacteria and viruses after the introduction of treated wastewater; these microbes also decreased over time resulting in the same strain clusters in control and treatment at the end of the experiment. Specifically, 39.2% of viral strains detected in all samples were present after the introduction of treated wastewater only. Although bacteria present at low abundance in the treated wastewater introduced additional antibiotic resistance genes, signals of naturally occurring ARG-encoding organisms resembled the resistome at the endpoint. Our results suggest that the previously stressed freshwater stream and its microbial community are resilient to a substantial introduction of treated wastewater.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1347","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9846259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A parasitic nematode induces dysbiosis in susceptible but not resistant gastropod hosts 寄生线虫在易感但不耐药的腹足类宿主中诱导生态失调

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-10 DOI: 10.1002/mbo3.1346

Laura Sheehy, Kerry MacDonald-Howard, Chris D. Williams, Gareth D. Weedall, Hayley Jones, Robbie Rae

{"title":"A parasitic nematode induces dysbiosis in susceptible but not resistant gastropod hosts","authors":"Laura Sheehy, Kerry MacDonald-Howard, Chris D. Williams, Gareth D. Weedall, Hayley Jones, Robbie Rae","doi":"10.1002/mbo3.1346","DOIUrl":"10.1002/mbo3.1346","url":null,"abstract":"Animals’ gut microbiomes affect a wide array of biological processes including immunity and protection from pathogens. However, how the microbiome changes due to infection by parasites is still largely unknown, as is how the microbiome changes in hosts that differ in their susceptibility to parasites. To investigate this, we exposed two slug species of differing susceptibility to the parasitic nematode Phasmarhabditis hermaphrodita (Deroceras reticulatum is highly susceptible and Ambigolimax valentianus resistant to the nematode) and profiled the gut microbiota after 7 and 14 days. Before infection, both slug species’ microbiota was dominated by similar bacterial genera: Pseudomonas (by far the most abundant), Sphingobacterium, Pedobacter, Chryseobacterium, and Flavobacterium. In the resistant host A. valentianus, there was no significant change in the bacterial genera after infection, but in D. reticulatum, the bacterial profile changed, with a decrease in the abundance of Pseudomonadaceae and an increase in the abundance of Flavobacteriaceae and Sphingobacteriaceae after 7 days postinfection. This suggests nematode infection causes dysbiosis in hosts that are susceptible to infection, but the microbiome of resistant species remains unaltered. In summary, the regulation of the immune system is tightly linked with host survival, and nematode infection can alter the microbiome structure.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9846264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Highly efficient export of a disulfide-bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli 在大肠杆菌中使用CyDisCo通过Tat途径高效地将二硫结合蛋白输出到外质和培养基中

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-08 DOI: 10.1002/mbo3.1350

Klaudia Arauzo-Aguilera, Mirva J. Saaranen, Colin Robinson, Lloyd W. Ruddock

{"title":"Highly efficient export of a disulfide-bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli","authors":"Klaudia Arauzo-Aguilera, Mirva J. Saaranen, Colin Robinson, Lloyd W. Ruddock","doi":"10.1002/mbo3.1350","DOIUrl":"10.1002/mbo3.1350","url":null,"abstract":"High-value heterologous proteins produced in Escherichia coli that contain disulfide bonds are almost invariably targeted to the periplasm via the Sec pathway as it, among other advantages, enables disulfide bond formation and simplifies downstream processing. However, the Sec system cannot transport complex or rapidly folding proteins, as it only transports proteins in an unfolded state. The Tat system also transports proteins to the periplasm, and it has significant potential as an alternative means of recombinant protein production because it transports fully folded proteins. Most of the studies related to Tat secretion have used the well-studied TorA signal peptide that is Tat-specific, but this signal peptide also tends to induce degradation of the protein of interest, resulting in lower yields. This makes it difficult to use Tat in the industry. In this study, we show that a model disulfide bond-containing protein, YebF, can be exported to the periplasm and media at a very high level by the Tat pathway in a manner almost completely dependent on cytoplasmic disulfide formation, by other two putative Tat SPs: those of MdoD and AmiC. In contrast, the TorA SP exports YebF at a low level.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9472333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The abundance of the potential pathogen Staphylococcus hominis in the air microbiome in a dental clinic and its susceptibility to far-UVC light 牙科诊所空气微生物群中潜在病原体人型葡萄球菌的丰度及其对远紫外线光的敏感性

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-05 DOI: 10.1002/mbo3.1348

Marilena Aquino de Muro, Igor Shuryak, Anne-Catrin Uhlemann, Alice Tillman, Dwayne Seeram, Joseph Zakaria, David Welch, Steven M. Erde, David J. Brenner

{"title":"The abundance of the potential pathogen Staphylococcus hominis in the air microbiome in a dental clinic and its susceptibility to far-UVC light","authors":"Marilena Aquino de Muro, Igor Shuryak, Anne-Catrin Uhlemann, Alice Tillman, Dwayne Seeram, Joseph Zakaria, David Welch, Steven M. Erde, David J. Brenner","doi":"10.1002/mbo3.1348","DOIUrl":"10.1002/mbo3.1348","url":null,"abstract":"The dental clinic air microbiome incorporates microbes from the oral cavity and upper respiratory tract (URT). This study aimed to establish a reliable methodology for air sampling in a dental clinic setting and quantify the abundance of culturable mesophilic aerobic bacteria present in these samples using regression modeling. Staphylococcus hominis, a potentially pathogenic bacterium typically found in the human oropharynx and URT, was consistently isolated. S. hominis was the most abundant species of aerobic bacteria (22%–24%) and comprised 60%–80% of all Staphylococcus spp. The study also assessed the susceptibility of S. hominis to 222 nm-far-UVC light in laboratory experiments, which showed an exponential surface inactivation constant of k = 0.475 cm2/mJ. This constant is a critical parameter for future on-site use of far-UVC light as a technique for reducing pathogenic bacterial load in dental clinics.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9846263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatic survey of CRISPR loci across 15 Serratia species 15种沙雷氏菌CRISPR基因座的生物信息学调查

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-03-02 DOI: 10.1002/mbo3.1339

Maria Scrascia, Roberta Roberto, Pietro D'Addabbo, Yosra Ahmed, Francesco Porcelli, Marta Oliva, Carla Calia, Angelo Marzella, Carlo Pazzani

{"title":"Bioinformatic survey of CRISPR loci across 15 Serratia species","authors":"Maria Scrascia, Roberta Roberto, Pietro D'Addabbo, Yosra Ahmed, Francesco Porcelli, Marta Oliva, Carla Calia, Angelo Marzella, Carlo Pazzani","doi":"10.1002/mbo3.1339","DOIUrl":"10.1002/mbo3.1339","url":null,"abstract":"The Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins (CRISPR–Cas) system of prokaryotes is an adaptative immune defense mechanism to protect themselves from invading genetic elements (e.g., phages and plasmids). Studies that describe the genetic organization of these prokaryotic systems have mainly reported on the Enterobacteriaceae family (now reorganized within the order of Enterobacterales). For some genera, data on CRISPR–Cas systems remain poor, as in the case of Serratia (now part of the Yersiniaceae family) where data are limited to a few genomes of the species marcescens. This study describes the detection, in silico, of CRISPR loci in 146 Serratia complete genomes and 336 high-quality assemblies available for the species ficaria, fonticola, grimesii, inhibens, liquefaciens, marcescens, nematodiphila, odorifera, oryzae, plymuthica, proteomaculans, quinivorans, rubidaea, symbiotica, and ureilytica. Apart from subtypes I-E and I-F1 which had previously been identified in marcescens, we report that of I-C and the I-E unique locus 1, I-E*, and I-F1 unique locus 1. Analysis of the genomic contexts for CRISPR loci revealed mdtN-phnP as the region mostly shared (grimesii, inhibens, marcescens, nematodiphila, plymuthica, rubidaea, and Serratia sp.). Three new contexts detected in genomes of rubidaea and fonticola (puu genes-mnmA) and rubidaea (osmE-soxG and ampC-yebZ) were also found. The plasmid and/or phage origin of spacers was also established.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1339","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9846260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mystery of the ice cold rose—Microbiome of an Arctic winter frost flower 冰冷玫瑰的奥秘——北极冬季霜花的微生物群

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-02-04 DOI: 10.1002/mbo3.1345

Stefan Thiele, Anna Vader, Lise Øvreås

引用次数: 0

A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis 单体ParA/Soj在枯草芽孢杆菌染色体动力学中的新作用

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-01-16 DOI: 10.1002/mbo3.1344

David M. Roberts

引用次数: 1

A new spray-based method for the in-vitro development of dry-surface biofilms 基于喷雾的体外培养干表面生物膜的新方法

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-01-11 DOI: 10.1002/mbo3.1330

Esther Christine, Claude Olive, Myriam Louisin, Moustapha Dramé, Karine Marion-Sanchez

{"title":"A new spray-based method for the in-vitro development of dry-surface biofilms","authors":"Esther Christine, Claude Olive, Myriam Louisin, Moustapha Dramé, Karine Marion-Sanchez","doi":"10.1002/mbo3.1330","DOIUrl":"10.1002/mbo3.1330","url":null,"abstract":"The inanimate environment immediately surrounding the patient in healthcare facilities is a reservoir of microorganisms embedded in dry-surface biofilms (DSB). These biofilms, first highlighted in 2012, are increasingly studied, but currently available in-vitro models only allow for the growth of semi-hydrated biofilms. We developed a new in-vitro method under actual dehydration conditions based on the hypothesis that surface contamination is mainly due to splashes of respiratory secretions. The main objective of this study was to show that the operating conditions we have defined allowed the growth of DSB with a methicillin resistant Staphylococcus aureus strain. The second objective was to show that extended-spectrum beta-lactamase-producing Enterobacteriaceae, that is, Klebsiella pneumoniae and Enterobacter cloacae were also able to grow such biofilms under these conditions. Monobacterial suspensions in sterile artificial saliva (SAS) were sprayed onto polyethylene surfaces. Nutrients and hydration were provided daily by spraying SAS enriched with 20% of Brain Heart Infusion broth. The primary outcome was mean surface coverage measured by image analysis after crystal violet staining. The method applied to S. aureus for 12 days resulted in reproducible and repeatable DSB consisting of isolated and confluent microcolonies embedded in extracellular polymeric substances as shown in scanning electron microscopy images. Similar DSB were obtained with both Enterobacteriaceae applying the same method. No interspecies variation was shown between the three strains in terms of surface coverage. These first trials are the starting point for a 3-year study currently in process.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1330","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9367407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Assessing pH-dependent activities of virulence factors secreted by Candida albicans 评估白色念珠菌分泌的毒力因子的ph依赖性活性

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-01-03 DOI: 10.1002/mbo3.1342

Asier Ramos-Pardo, Rocío Castro-Álvarez, Guillermo Quindós, Elena Eraso, Elena Sevillano, Vladimir R. Kaberdin

{"title":"Assessing pH-dependent activities of virulence factors secreted by Candida albicans","authors":"Asier Ramos-Pardo, Rocío Castro-Álvarez, Guillermo Quindós, Elena Eraso, Elena Sevillano, Vladimir R. Kaberdin","doi":"10.1002/mbo3.1342","DOIUrl":"10.1002/mbo3.1342","url":null,"abstract":"Candida albicans is an opportunistic pathogen that can thrive under adverse conditions including suboptimal pH, nutrient scarcity, and low levels of oxygen. Its pathogenicity is associated with the production of virulence factors such as extracellular hydrolytic enzymes and toxins. This study was aimed at determining the effect of external pH, substrate nature, and strain origin on protease, lipase, and hemolysin production. To achieve this objective, agar plate assays were performed at pH 5.0, 6.5, and 7.5 with substrates suitable for the detection of each family of enzymes. Moreover, the study was conducted with 20 clinical C. albicans isolates from blood, oral cavity, skin, urine, and vagina. The hydrolytic zones formed around the colonies were further measured to calculate the Ez (enzymatic zone) indexes. We found that detection of proteases in skim milk agar plates was possible for most isolates only at pH 5 (80%) and pH 6.5 (75%), whereas BSA plates could confer protease detection exclusively at pH 5 (80%). Similarly, the percentage of isolates possessing lipolytic activities was higher at pH 5 (90%) than at pH 6.5 (70%) and pH 7.5 (35%). In contrast, hemolytic activities were detected in all isolates at pH 6.5 and 7.5 but not at pH 5. Further analysis revealed that some differences in the detected activities could potentially be attributed to the anatomical origin of these isolates. Collectively, these findings suggest that the pH of the site of infection might be critical for mimicking the microenvironment employed to experimentally discover the key virulence factors.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1342","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9072648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Adaptive laboratory evolution for increased temperature tolerance of the diatom Nitzschia inconspicua 提高硅藻耐温性的适应性实验室进化

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2022-12-25 DOI: 10.1002/mbo3.1343

Alaina J. LaPanse, Tyson A. Burch, Jacob M. Tamburro, Jesse C. Traller, Agnieszka Pinowska, Matthew C. Posewitz

{"title":"Adaptive laboratory evolution for increased temperature tolerance of the diatom Nitzschia inconspicua","authors":"Alaina J. LaPanse, Tyson A. Burch, Jacob M. Tamburro, Jesse C. Traller, Agnieszka Pinowska, Matthew C. Posewitz","doi":"10.1002/mbo3.1343","DOIUrl":"10.1002/mbo3.1343","url":null,"abstract":"Outdoor microalgal cultivation for the production of valuable biofuels and bioproducts typically requires high insolation and strains with high thermal (>37°C) tolerance. While some strains are naturally thermotolerant, other strains of interest require improved performance at elevated temperatures to enhance industrial viability. In this study, adaptive laboratory evolution (ALE) was performed for over 300 days using consecutive 0.5°C temperature increases in a constant temperature incubator to attain greater thermal tolerance in the industrially relevant diatom Nitzschia inconspicua str. Hildebrandi. The adapted strain was able to grow at a constant temperature of 37.5°C; whereas this constant temperature was lethal to the parental control, which had an upper-temperature boundary of 35.5°C before adaptive evolution. Several high-temperature clonal isolates were obtained from the evolved population following ALE, and increased temperature tolerance was observed in the clonal, parent, and non-clonal adapted cultures. This ALE method demonstrates the development of enhanced industrial algal strains without the production of genetically modified organisms (GMOs).","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1343","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9367408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0