Medycyna doswiadczalna i mikrobiologia最新文献

[Staphylococcus infection in a multi-profile hospital in Nowy Targ, Poland, in the years 2001-2004 - analysis of antibiotic resistance]. [2001-2004年波兰Nowy Targ一家综合性医院的葡萄球菌感染——抗生素耐药性分析]。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2017-01-01

Wioletta Waksmahska, Andrzej Wiczkowski, Rafal Bobihski, Anna Glemp-Migief

{"title":"[Staphylococcus infection in a multi-profile hospital in Nowy Targ, Poland, in the years 2001-2004 - analysis of antibiotic resistance].","authors":"Wioletta Waksmahska, Andrzej Wiczkowski, Rafal Bobihski, Anna Glemp-Migief","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Global concern is the treatment of infections caused by methicillin-resistant strains ofgenus Staphylococcus. The aim ofthis study was the analysis ofthe staphylococcal infections' incidence in a multi-profile hospital in Nowy Targ, Poland, in the years 2001- 2004 with a focus on the occurrence of antimicrobial resistance among isolated strains of S. aureus and methicillin-resistant staphylococci.Material and method: The study was based on the results of bacteriological tests performed in the hospital bacteriological laboratory. The study included patients treated in years 2001-2004 in cardiology, nephrology, surgery, orthopedics, pediatric, intensive care, gynecology and neonatal ward.Results: Regardless of the year in which the analysis was performed, S. aureus strains resistant to methicillin were not cultured on the neonatal ward and gynecology ward. On the other side, methicillin-sensitive strains were cultured on all of the hospital's wards. A very high sensitivity (virtually 100%) of staphylococcus to vancomycin and teicoplanin and a high sensitivity (87-93%) to chloramphenicol was found. This study showed that the methicillin-resistant Staphylococcus strains were the least sensitive to tetracycline.Conclusions: 1. The highest sensitivity of Staphylococcus was reported to glycopeptides and the lowest to tetracycline. 2. Most of the Staphylococcus strains were cultured in the cardiology department and the least of the strains in the department of gynecology. 3. It is advisable to check whether the frequency of Staphylococcus culture's occurrence has decreased after implementation of the WHO recommendations.","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"69 1","pages":"5-13"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36654636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Serological and molecular methods in diagnostics of primary acute hepatitis caused with human herpesvirus type 6 - a case report]. [用血清学和分子方法诊断6型人疱疹病毒引起的原发性急性肝炎1例报告]。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2017-01-01

Marta Rybka, Maciej Przybylski, Paulina Machura, Tomasz Dzieciątkowski, Rafal Krenke', Marta Wrólewska

{"title":"[Serological and molecular methods in diagnostics of primary acute hepatitis caused with human herpesvirus type 6 - a case report].","authors":"Marta Rybka, Maciej Przybylski, Paulina Machura, Tomasz Dzieciątkowski, Rafal Krenke', Marta Wrólewska","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: HHV-6 has been identified as the etiologic agent of exanthema subitum in infants and acute febrile illness in young children, although primary HHV-6 infection in immunocompetent adolescents and adults is very rare. We report the case of acute hepatitis in an 18-year-old man without any immunological dysfunctions.Material and methods: Full virological examination of sera samples was performed. Specimens were tested for the presence of specific anti-EBV and anti-HHV-6 antibodies and also for the presence of DNA of other herpesviruses, using qPCR assays.Results: Obtained results demonstrated the presence of HHV-6 DNA in all examined sera samples and IgM-class antibodies against HHV-6 in the second specimen. No other etiologic agents were found that are known to induce hepatitis. Hence, patient was diagnosed as having acute hepatitis triggered by HHV-6.Conclusions: We found that qPCR is a very useful tool for the detection of different herpesvirus infections, especially with low-copy viraemia in clinical specimens.","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"69 1","pages":"43-48"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36609943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The prevalence of human papillomaviruses in patients with colon polyps]. 【人乳头瘤病毒在结肠息肉患者中的流行】。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2017-01-01

Beata Mlynarczyk-Bonikowska, Jacek Muszyński, Ksenia Szymanek-Mjchrzak, Bartomiej Ziółowski, Graiyna Mlynarczyk, Magdalena Malejczyk, Lawomir Majewski

{"title":"[The prevalence of human papillomaviruses in patients with colon polyps].","authors":"Beata Mlynarczyk-Bonikowska, Jacek Muszyński, Ksenia Szymanek-Mjchrzak, Bartomiej Ziółowski, Graiyna Mlynarczyk, Magdalena Malejczyk, Lawomir Majewski","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Potential role of HPV infection in pathogenesis of colon polyps and cancer remains undetermined. The aim of the study was to investigate the prevalence of DNA of HPV- 6, -11, -16 and -18 in the biopsies from colon polyps.Material and methods: We investigated the biopsies from 24 patients (23 from colon polyps and I from colon cancer) of Department of Gastroenterology Medical University of Warsaw using Real time PCR HPV-6/11. Real-TM (Sacace Biotechnologies) was performed on termocycler Smart Cycler Dx.Results: We didn't detect oncogenic HPV16 and HPV18 in any of the investigated specimens, HPV-11 was present in 11 patients including all patients with adenoma tubule-villosum. We detect HPV6 in 5 samples from polyps and 1 from colon cancer.Conclusions: HPV 6 and HPV 11 could play a role in pathogenesis some colon polyps but the final conclusions demand further investigations. Oncogenic HPV 16 and 18 probably don't play any role in colon polyps pathogenesis.","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"69 1","pages":"49-54"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36609946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular characterization of Staphylococcus aureus strains isolated from surgical site infections of patients in southern Poland]. [波兰南部手术部位感染患者分离的金黄色葡萄球菌菌株的分子特征]。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2017-01-01

Monika Pomorska-Wesolowska, Katarzyna Malyzek, Dorota Romaniszyn, Monika Pobiega, Anna Szczypta, Miroslawa Dzikowska, Agnieszka Chmielarczyk, Anna Różańska, Joanna Maciąg, Jadwiga Wójkowska-Mach

{"title":"[Molecular characterization of Staphylococcus aureus strains isolated from surgical site infections of patients in southern Poland].","authors":"Monika Pomorska-Wesolowska, Katarzyna Malyzek, Dorota Romaniszyn, Monika Pobiega, Anna Szczypta, Miroslawa Dzikowska, Agnieszka Chmielarczyk, Anna Różańska, Joanna Maciąg, Jadwiga Wójkowska-Mach","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: The aim of the study was a molecular characterization of Staphylococcus aureus strains isolated from surgical site infections (SSIs) from patients in southern Poland, undergoing different surgical procedures, together with evaluation of the prevalence of antimicrobial resistance and the presence of virulence factors.Materials and methods: In this laboratory-based, multicenter study, non-repetitive 162 samples from SSI were collected from hospitalized patients (12 hospitals, n=139) or outpatients (n=23) in southern Poland between January 1 and December 31, 2013. In all S. aureus isolates, we investigated antimicrobial susceptibility, the presence of selected virulence genes (lukE, pvl, tsst-l and eta), and also conducted spa typing.Results: Patients with SSI had a median age of 61 years; 54.9% were male. Prevalence of MRSA (29 strains, 17.9%) SSI per surgery type was 8.7% in orthopaedic, 17.7% in general and 42.9% in vascular surgery. Over 20% of strains were resistant for erythromycin (27.2%), clindamycin (23.5%). No resistance was found for linezolid, glycopeptides or tigecycline. Gene of Leukocidin (lukE) was the most frequently found gene. Spa typing identified 10 spa types; the two dominant types were t003 (41.4%) and t138(17.2%).Conclusions: The results show that after vascular surgery, there was an unexpectedly high prevalence of MRSA in SSIs in southern Poland. Conversely, the prevalence of MRSA was unexpectedly low following orthopaedics procedures. The surprisingly observation was the low virulence of the S. aureus strains among older patients.","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"69 1","pages":"15-25"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36609941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Diagnostic difficulties in amebic liver abscess]. 阿米巴肝脓肿的诊断困难。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2017-01-01

Jacek Czepiel, Martyna Gomulska, Graźyna Biesiada, Iwona Sobczyk-Krupiarz, Roksana Kisiel, Aleksander Garlicki

引用次数: 0

[The occurrence of infections caused by Francisella tularensis in humans in Poland and laboratory diagnosis of tularemia]. [波兰由土拉菌引起的人类感染的发生和土拉菌病的实验室诊断]。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2017-01-01

Anna Chróst, Kornelia Gielarowiec, Stanislaw Kalużewski, Waldemar Rastawicki

{"title":"[The occurrence of infections caused by Francisella tularensis in humans in Poland and laboratory diagnosis of tularemia].","authors":"Anna Chróst, Kornelia Gielarowiec, Stanislaw Kalużewski, Waldemar Rastawicki","doi":"","DOIUrl":"","url":null,"abstract":"Tularemia is a serious infectious zoonotic disease, caused by Gram-negative bacterium Francisella tularensis. Natural reservoir of infection are small mammals such a mice, voles, squirrels and rabbits. Transmission to humans occurs through contact with infected animals or contaminated environments, or through arthropod vectors. Because of its extreme infectivity it is a dangerous biological agent to human health. Tularemia has a broad geographical distribution, however is mainly in the northern hemisphere, in areas with cooler climates particularly in North America, Europe, Russia and Japan. Most of the cases among European countries have been reported in the Scandinavian region. The prevalence rate of tularemia in Poland is small, although in recent years stable increase has been observed. According to official epidemiological data during the years 2010-2016 only 61 cases of tularemia were reported in Poland. A laboratory diagnosis of tularemia is based on serological investigation, classical microbiology and molecular biology. The aim of this study was to evaluate the prevalence of infections caused by Francisella tularensis in humans in Poland and present characteristics of laboratory diagnosis of tularemia.","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"69 1","pages":"55-63"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36609948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Health risks associated with the environmental presence of toxigenic moulds]. [与环境中存在的产毒霉菌有关的健康风险]。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2016-01-01

Anna Chróst

引用次数: 0

[Helicobacter pylori - characteristics of pathogen, pathogenesis and microbiological diagnosis of infections]. 幽门螺杆菌——病原菌特征、发病机制及感染的微生物学诊断。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2016-01-01

Kornelia Gielarowiec

引用次数: 0

[Comparison of three multiplex real-time PCR assays for the detection of herpes simplex viruses type 1 and type 2 DNA]. 三种多重实时PCR检测单纯疱疹病毒1型和2型DNA的比较

Medycyna doswiadczalna i mikrobiologia Pub Date : 2016-01-01

Anna Majewska, Maciej Przybylski, Paulina Machura, Graźyna Mlynarczyk, Tomasz Dzieciqtkowski

{"title":"[Comparison of three multiplex real-time PCR assays for the detection of herpes simplex viruses type 1 and type 2 DNA].","authors":"Anna Majewska, Maciej Przybylski, Paulina Machura, Graźyna Mlynarczyk, Tomasz Dzieciqtkowski","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Herpes simplex viruses type 1 and type 2 (HSV-1 and HSV-2) cause widespread infection worldwide with different course and intensity. Although the disease caused by this viruses mainly concern healthy children and adults, the HSV infections are much more dangerous for people with immunodeficiencies. The aim of this work was to compare the diagnostic value of two qPCR methods for detection HSV-1/2 DNA, based on TaqMan* and HybProbes chemistry with commercial HSV-1/2 Qual Kit.Methods: DNA from 51 clinical samples was tested for presence of HSV-1/2 sequences on LightCycler 2.0 thermocycler, using two ,,in-house\" developed multiplex real-time PCR assays and commercial test using SCORPIONSTM primers.Results: The results showed high specificity and sensitivity of all molecular biology tests used. Statistically, there was no significant difference in the sensitivity of real-time PCR assays when using TaqMan* and HybProbes' chemistry and when using the commercial SCORPIONSTM based method (P>0.05).Conclusions: Obtained results show that all tested methods are highly specific and can possibly be used to simultaneously detect and differentiate HSV-1/2 DNA in clinical samples. The high detection rate and short duration of qPCR assayas has great importance for immunocompromised patients where quick application of effective and safe treatment is necessary. It is also important in the event of amorphous form of the infection and the occurrence of nonspecific and generalized symptoms.","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"68 2","pages":"119-125"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36609399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Prevalence of CI-HHV-6 in patients with hematological diseases and healthy donors in Poland - preliminary study]. [CI-HHV-6在波兰血液病患者和健康献血者中的流行-初步研究]。

Medycyna doswiadczalna i mikrobiologia Pub Date : 2016-01-01

Magdalena Tormanowska, Tomasz Dzieciątkowski, Sylwia Rynans, Maciej Przybylski, Anna Gronkowska, Grzegorz W Basak, Graźyna Młynarczyk, Agnieszka Tomaszewska

引用次数: 0